sp|Q9SAR5|AKR2A_ARATH ----> Ankyrin repeat domain-containing protein 2A

Intron 1 GTAAGTTTTA ... CTTCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.11	417	52	105
A2	21.15	410	60.4	110
A3	17.45	492	77.2	104
A4	18.55	303	40	69
A5	14.98	261	42.4	46
A6	20.83	3869.2	577	1018
A7	19.71	3570.2	579.4	876
A8	22.95	3948.8	538.2	1176
A9	19.21	3293.8	518.6	783
A10	17.01	2526.8	398	518

>TRINITY_DN41713_c0_g1_i2 from sample A7 / Example spliced isoform

CTCAAGGCAAGCCTAGGTGTGGTAGGGAAAGGGCACAAGAAAAAGGAAAGGATGTGGAGATAAGATGGTGTTTCAATGCACACGTATGGACCCATGTGGAATCTGTAACTGAGCTTGAGTTTTATGATTATATTTTATTATTATTTAAATATTAAACAAACGAAGTTAAATTTATAACCGAAGGATCGGTACCAGCCATG
CAGTTACCAGAGTGTGCAATCAAATTGAGGATCTGCAAGAGCTAGTTGTAAAGTCCCTAAAACCCTTTTCACGACCTTGAAAACATTAGGAAGACAGCAATAAACTCTCGGGGACTCTTATCTCTCTCACGTCCTTAAACGACAGAGGAAAAACAGGGGGAAGAGGGAATCTGTGATCTGCTGTTTCTTCCAAATCTGCG
GAGGATTTACTGTATTTGCAGCAGCCATGGCCTCCGCAGAGGAAAAGTCAAGTGCATCTGGCAGTAAGGGAGCTAAGACAGGGACATCATCATCTCAACCAAAAACTCCTACGAGCAGTGGACGCACAGTGCCTTCCTTTGTGCCAGAGACGGGGATATCACCAAATCCATTTGATTTCTCTGCAATGTCTGGCCTACTT
AATGATCCCAGCATAAAGGATTTGGCAGAGCAGATAGCGAAGGATCCATCATTCAATCAGATGGCAGAGCAACTTCAGCAGAGTGTGCAGCATGCAGGGCAAGATGGCATTCCACAGCTGGATACACAGCAGTATTTCTCTACAATGCAACAGGTTATGCAGAATCCTCAGTTCATGACCATGGCTGAACGGCTTGGTAA
TGCTCTCATGCAGGATCCATCCATGTCCAGTATGTTGGAGAATCTTTCCAATCCTGCTCACAAAGAACAGTTGGAGGAGCGCATGGCAAATATTAAGGAAGATCCATCTTTGAAACCTATCCTCGAGGAGATTGAATCTGGGGGACCAGCTGCAATGATGAAGTACTGGAATGATCCGGCAGTACTGACCAAGCTAGGTC
AGGCAATGGGTGTTGGAATTTCTGGTGATATGCCTGCCTCCAATGAGGGAACTGGTGCAGATGAAGGTGAAGAGGATAATGGGGATGAGGATGATGATGAGCTCACTGTTCACCACACTGCAAGTGCTGGTGATGTACAGGGATTGAAGAATGTCCTCGCAATGGGTGCAGACAAAGATGAGGCAGACTCCGAGGGCCGA
ACAGCTTTGCACTTTGCTTGTGGGTATGGTGAGGTGAAATGCGCTGAAGTACTTCTTGAAGCAGGTGCAGCTGTTGATGCACTTGATAAGAACAAGAACACGGCTCTTCATTATGCAGCTGGCTATGGACGGAAAGAATGTGTAGAACTTCTTTTGAAGCACGGAGCAGCTGTGACCCTTCAAAACTTGGATGGGAAAAC
ACCTATTGATGTCGCCAAACTGAACTCTCAGGAGGCAGTTTTGAAGCTGCTGGAGAAAGATGCATTTTTGTAGTCTCGAGTAATTAAAATTTGTTTAAAATTTCTGTAATACAATCACAAGTGTTTCTTAGCTGGATGGTTGGGCTGTCTTGGTTCACAAGGTTTGCTGCATTTTCAAAAATCGAAATGACATTTGCAAG
TGTTTCAGCATTTTAGTAGTTCAACTGGGCAGTTGCTTTGATCTGCACCCATTGTGGATTAGGCTGGGCTCATATGTTCCAACGTTCTTGTTATTACCTTATGTAAGGTTATAATGTATTTATTTATTATTATTGTATTTCTCTATGTTCTGCATTTCTGGGGAAGTTTAATTTTACTGCAAACAAAAGATTACATACAG
TTTGCAAATTAGAGTCCTTTGCTAAAATTGAATTATCTCTGTTTCTTCTGTGAATTTACTGTCTCTTTCTCTTTGCTTATGGCATGTGCTTGAGACTTCCCTTCTTATTCAATAGCATTTTATTGTATTTACTTATGGCATGTGCGTGAGACTTCCCTACTTATTCAATAGCATTTTATTGTATTTACTTATGGCATGTG
CGTGAGACTTCCCTACTTATTCAATAGCATTTTATTGT

>TRINITY_DN41713_c0_g1_i4 from sample A7 / Example unspliced isoform:

CTCAAGGCAAGCCTAGGTGTGGTAGGGAAAGGGCACAAGAAAAAGGAAAGGATGTGGAGATAAGATGGTGTTTCAATGCACACGTATGGACCCATGTGGAATCTGTAACTGAGCTTGAGTTTTATGATTATATTTTATTATTATTTAAATATTAAACAAACGAAGTTAAATTTATAACCGAAGGATCGGTACCAGCCATG
CAGTTACCAGAGTGTGCAATCAAATTGAGGATCTGCAAGAGCTAGTTGTAAAGTCCCTAAAACCCTTTTCACGACCTTGAAAACATTAGGAAGACAGCAATAAACTCTCGGGGACTCTTATCTCTCTCACGTCCTTAAACGACAGAGGAAAAACAGGGGGAAGAGGGAATCTGTGATCTGCTGTTTCTTCCAAATCTGCG
GAGGATTTACTGTATTTGCAGCAGCCATGGCCTCCGCAGAGGAAAAGTCAAGTGCATCTGGCAGTAAGGGAGCTAAGACAGGGACATCATCATCTCAACCAAAAACTCCTACGAGCAGTGGACGCACAGTGCCTTCCTTTGTGCCAGAGACGGGGATATCACCAAATCCATTTGATTTCTCTGCAATGTCTGGCCTACTT
AATGATCCCAGCATAAAGGATTTGGCAGAGCAGATAGCGAAGGATCCATCATTCAATCAGATGGCAGAGCAACTTCAGCAGAGTGTGCAGCATGCAGGGCAAGATGGCATTCCACAGCTGGATACACAGCAGTATTTCTCTACAATGCAACAGGTTATGCAGAATCCTCAGTTCATGACCATGGCTGAACGGCTTGGTAA
TGCTCTCATGCAGGATCCATCCATGTCCAGTATGTTGGAGAATCTTTCCAATCCTGCTCACAAAGAACAGTTGGAGGAGCGCATGGCAAATATTAAGGAAGATCCATCTTTGAAACCTATCCTCGAGGAGATTGAATCTGGGGGACCAGCTGCAATGATGAAGTACTGGAATGATCCGGCAGTACTGACCAAGCTAGGTC
AGGCAATGGGTGTTGGAATTTCTGGTGATATGCCTGCCTCCAATGAGGGAACTGGTGCAGATGAAGGTGAAGAGGATAATGGGGATGAGGATGATGATGAGCTCACTGTTCACCACACTGCAAGTGCTGGTGATGTACAGGGATTGAAGAATGTCCTCGCAATGGGTGCAGACAAAGATGAGGCAGACTCCGAGGGCCGA
ACAGCTTTGCACTTTGCTTGTGGGTATGGTGAGGTGAAATGCGCTGAAGTACTTCTTGAAGCAGGTGCAGCTGTTGATGCACTTGATAAGAACAAGAACACGGCTCTTCATTATGCAGCTGGCTATGGACGGAAAGAATGTGTAGAACTTCTTTTGAAGCACGGAGCAGCTGTgtaagttttaattccttggaatatata
tatggaatatgtatactggctctttcatgccagatatatgtgagtaaacaccagaacatgttttggagtcttgtacttaaatgttttgcttcttgcagGACCCTTCAAAACTTGGATGGGAAAACACCTATTGATGTCGCCAAACTGAACTCTCAGGAGGCAGTTTTGAAGCTGCTGGAGAAAGATGCATTTTTGTAGTC
TCGAGTAATTAAAATTTGTTTAAAATTTCTGTAATACAATCACAAGTGTTTCTTAGCTGGATGGTTGGGCTGTCTTGGTTCACAAGGTTTGCTGCATTTTCAAAAATCGAAATGACATTTGCAAGTGTTTCAGCATTTTAGTAGTTCAACTGGGCAGTTGCTTTGATCTGCACCCATTGTGGATTAGGCTGGGCTCATAT
GTTCCAACGTTCTTGTTATTACCTTATGTAAGGTTATAATGTATTTATTTATTATTATTGTATTTCTCTATGTTCTGCATTTCTGGGGAAGTTTAATTTTACTGCAAACAAAAGATTACATACAGTTTGCAAATTAGAGTCCTTTGCTAAAATTGAATTATCTCTGTTTCTTCTGTGAATTTACTGTCTCTTTCTCTTTG
CTTATGGCATGTGCTTGAGACTTCCCTTCTTATTCAATAGCATTTTATTGTATTTACTTATGGCATGTGCGTGAGACTTCCCTACTTATTCAATAGCATTTTATTGTATTTACTTATGGCATGTGCGTGAGACTTCCCTACTTATTCAATAGCATTTTATTGT

Intron 2 GTATGGACCC ... AGCCATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.69	11	3	15
A2	64.29	15	3	27
A3	58.82	7	3	10
A4	61.76	13	8	21
A5	66.67	3	4	6
A6	61.76	26	9	42
A7	58.4	52	14	73
A8	63.77	25	10	44
A9	59.84	49	14	73
A10	59.66	48	11	71

>TRINITY_DN98593_c0_g1_i2 from sample A4 / Example spliced isoform

GATTAGGGTAGCCCACCTCTCAAGGCAAGCCTAGGTGTGGTAGGGAAAGGGCACAAGAAAAAGGAAAGGATGTGGAGATAAGATGGTGTTTCAATGCACACTTACCAGAGTGTGCAATCAAATTGAGGATCTGCAAGAGCTAGTTGTAAAGTCCCTAAAACCCTTTTCACGACCTTGAAAACATTAGGAAGACAGCAATA
AACTCTCGGGGACTCTTATCTCTCTCACGTCCTTAAACGACAGAGGAAAAACAGGGGGAAGAGGGAATCTGTGATCTGCTGTTTCTTCCAAATCTGCGGAGGATTTACTGTATTTGCAGCAGCCATGGCCTCCGCAGAGGAAAAGTCAAGTGCATCTGGCAGTAAGGGAGCTAAGACAGGGACATCATCATCTCAACCAA
AAACTCCTACGAGCAGTGGACGCACAGTGCCTTCCTTTGTGCCAGAGACGGGGATATCACCAAATCCATTTGATTTCTCTGCAATGTCTGGCCTACTTAATGATCCCAGCATAAAGGATTTGGCAGAGCAGATAGCGAAGGATCCATCATTCAATCAGATGGCAGAGCAACTTCAGCAGAGTGTGCAGCATGCAGGGCAA
GATGGCATTCCACAGCTGGATACACAGCAGTATTTCTCTACAATGCAACAGGTTATGCAGAATCCTCAGTTCATGACCATGGCTGAACGGCTTGGTAATGCTCTCATGCAGGATCCATCCATGTCCAGTATGTTGGAGAATCTTTCCAATCCTGCTCACAAAGAACAGTTGGAGGAGCGCATGGCAAATATTAAGGAAGA
TCCATCTTTGAAACCTATCCTCGAGGAGATTGAATCTGGGGGACCAGCTGCAATGATGAAGTACTGGAATGATCCGGCAGTACTGACCAAGCTAGGTCAGGCAATGGGTGTTGGAATTTCTGGTGATATGCCTGCCTCCAATGAGGGAACTGGTGCAGATGAAGGTGAAGAGGATAATGGGGATGAGGATGATGATGAGC
TCACTGTTCACCACACTGCAAGTGCTGGTGATGTACAGGGATTGAAGAATGTCCTCGCAATGGGTGCAGACAAAGATGAGGCAGACTCCGAGGGCCGAACAGCTTTGCACTTTGCTTGTGGGTATGGTGAGGTGAAATGCGCTGAAGTACTTCTTGAAGCAGGTGCAGCTGTTGATGCACTTGATAAGAACAAGAACACA
GCTCTTCATTATGCAGCTGGCTATGGACGGAAAGAATGTGTAGAACTTCTTTTGAAGCACGGAGCAGCTGTGACCCTTCAAAACTTGGATGGGAAAACACCTATTGATGTCGCCAAACTGAACTCTCAGGAGGCAGTTTTGAAGCTGCTGGAGAAAGATGCATTTTTGTAGTCTCGAGTAATTAAAATTTGTTTAAAATT
TCTGTAATACAATCACAAGTGTTTCTTAGCTGGATGGTTGGGCTGTCTTGGTTCACAAGGTTTGCTGCATTTTCAAAAATCGAAATGACATTTGCAAGTGTTTCAGCATTTTAGTAGTTCAACTGGGCAGTTGCTTTGATCTGCACCCATTGTGGATTAGGCTGGGCTCATATGTTCCAACGTTCTTGTTATTACCTTAT
GTAAGGTTATAATGTATTTATTTATTATTATTGTATTTCTCTATGTTCTGCATTTCTGGGGAAGTTTAATTTTACTGCAAACAAAAGATTACATACAGTTTGCAAATTAGAGTCCTTTGCTAAAATTGAATTATCTCTGTTTCTTCTGTGAATTTACTGTCTCTTTCTCTTTGCTTATGGCATGTGCGTGAGACTTCCCT
TCTTATTCAATAGCATTTTATTGTATTTACTTATGGCATGTGCATGAGACCTCCCTTCTTATTCAATAGCATTTTATTGTATTTACTTATGGCATGTGCATGAGACCTCCCTTCTTATTCAATAGCATTT

>TRINITY_DN98593_c0_g1_i3 from sample A4 / Example unspliced isoform:

GATTAGGGTAGCCCACCTCTCAAGGCAAGCCTAGGTGTGGTAGGGAAAGGGCACAAGAAAAAGGAAAGGATGTGGAGATAAGATGGTGTTTCAATGCACACgtatggacccatgtggaatctgtaactgagcttgagttttggtgattatattttattattatttaaatattaaacaaacgaagttaaatttataaccga
aggatcggtaccagccatgcagTTACCAGAGTGTGCAATCAAATTGAGGATCTGCAAGAGCTAGTTGTAAAGTCCCTAAAACCCTTTTCACGACCTTGAAAACATTAGGAAGACAGCAATAAACTCTCGGGGACTCTTATCTCTCTCACGTCCTTAAACGACAGAGGAAAAACAGGGGGAAGAGGGAATCTGTGATCTGC
TGTTTCTTCCAAATCTGCGGAGGATTTACTGTATTTGCAGCAGCCATGGCCTCCGCAGAGGAAAAGTCAAGTGCATCTGGCAGTAAGGGAGCTAAGACAGGGACATCATCATCTCAACCAAAAACTCCTACGAGCAGTGGACGCACAGTGCCTTCCTTTGTGCCAGAGACGGGGATATCACCAAATCCATTTGATTTCTC
TGCAATGTCTGGCCTACTTAATGATCCCAGCATAAAGGATTTGGCAGAGCAGATAGCGAAGGATCCATCATTCAATCAGATGGCAGAGCAACTTCAGCAGAGTGTGCAGCATGCAGGGCAAGATGGCATTCCACAGCTGGATACACAGCAGTATTTCTCTACAATGCAACAGGTTATGCAGAATCCTCAGTTCATGACCA
TGGCTGAACGGCTTGGTAATGCTCTCATGCAGGATCCATCCATGTCCAGTATGTTGGAGAATCTTTCCAATCCTGCTCACAAAGAACAGTTGGAGGAGCGCATGGCAAATATTAAGGAAGATCCATCTTTGAAACCTATCCTCGAGGAGATTGAATCTGGGGGACCAGCTGCAATGATGAAGTACTGGAATGATCCGGCA
GTACTGACCAAGCTAGGTCAGGCAATGGGTGTTGGAATTTCTGGTGATATGCCTGCCTCCAATGAGGGAACTGGTGCAGATGAAGGTGAAGAGGATAATGGGGATGAGGATGATGATGAGCTCACTGTTCACCACACTGCAAGTGCTGGTGATGTACAGGGATTGAAGAATGTCCTCGCAATGGGTGCAGACAAAGATGA
GGCAGACTCCGAGGGCCGAACAGCTTTGCACTTTGCTTGTGGGTATGGTGAGGTGAAATGCGCTGAAGTACTTCTTGAAGCAGGTGCAGCTGTTGATGCACTTGATAAGAACAAGAACACAGCTCTTCATTATGCAGCTGGCTATGGACGGAAAGAATGTGTAGAACTTCTTTTGAAGCACGGAGCAGCTGTGACCCTTC
AAAACTTGGATGGGAAAACACCTATTGATGTCGCCAAACTGAACTCTCAGGAGGCAGTTTTGAAGCTGCTGGAGAAAGATGCATTTTTGTAGTCTCGAGTAATTAAAATTTGTTTAAAATTTCTGTAATACAATCACAAGTGTTTCTTAGCTGGATGGTTGGGCTGTCTTGGTTCACAAGGTTTGCTGCATTTTCAAAAA
TCGAAATGACATTTGCAAGTGTTTCAGCATTTTAGTAGTTCAACTGGGCAGTTGCTTTGATCTGCACCCATTGTGGATTAGGCTGGGCTCATATGTTCCAACGTTCTTGTTATTACCTTATGTAAGGTTATAATGTATTTATTTATTATTATTGTATTTCTCTATGTTCTGCATTTCTGGGGAAGTTTAATTTTACTGCA
AACAAAAGATTACATACAGTTTGCAAATTAGAGTCCTTTGCTAAAATTGAATTATCTCTGTTTCTTCTGTGAATTTACTGTCTCTTTCTCTTTGCTTATGGCATGTGCGTGAGACTTCCCTTCTTATTCAATAGCATTTTATTGTATTTACTTATGGCATGTGCATGAGACCTCCCTTCTTATTCAATAGCATTTTATTG
TATTTACTTATGGCATGTGCATGAGACCTCCCTTCTTATTCAATAGCATTT