NA ----> NA

Intron 1 ATTTATCAAC ... TTTTGACAAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.33	60	91	77
A2	49.77	54	68	39
A3	60.75	52	91	70
A4	57.67	40	64	45
A5	69.49	9	21	20
A6	71.98	29	89	60
A7	60.58	95	158	134
A8	59.85	54	93	68
A9	64.42	58	129	81
A10	64.58	82	156	143

>TRINITY_DN122604_c0_g1_i8 from sample A2 / Example spliced isoform

CAATTTACTCTATACATGCACACATTGGATCAATGTGTAGAAGGATAGCAACATTGGACATAACAAGAAGGTTTTCACAACCCACGTTATAATGATTGACAAGATTAAAGCTTTTAAACGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTGTGTGAAAAAATTTCTGCTCTAAAGTAGACAACATTTATCA
AGCAACACTTTTTGACAACATTTATCAACTACTGTGTGAAAGTTCATTTCGGCATGAATAAGTTTCTGCTCCAAAGCAGCAACACTTTTGGACAACATTTAAATGTTTGCA

>TRINITY_DN122604_c0_g1_i1 from sample A2 / Example unspliced isoform:

TGAAAATGCCAAATTCAACACCGTGTTTTGAATATGTTTGGATTGCTGGTGACTGTGAAAATGCTAAATTCAACACTTTGTTTTGGATAAGCTTGGATTGCTGAATCTGCTGTACTTGTTGTATTCACAGCAATTTACCCAAAAATGCACACGTTGGATTGCTGGCAAGTTGGATTGTTGGTATTGCTGGCAAGTTGGAA
TGTTGGTAAATGGTGAAAATGTCAGATTCAGGATTCTTGGGAAATGGTGAAAATGCCAGATTCAACACCATGTCATGAATAAGTATAGATTGCTGAATTACCCAAAAATGCACACGTTGGATTTTGTTGGCAAGCTGGAATGTTGGTAAATGGTGAAAATGTCAGATTCAACACCATGTCATGAAGCTGGCAAGTTGGAA
TGTTGGTAAATGGTGAAATAATTACAGATTGTTGAATCTGTTGTATCTGTCATATTCACAACAATTTACTCTATACATGCACACATTGGATCAATGTGTAGAAGGATAGCAACATTGGACATAACAAGAAGGTTTTCACAACCCACGTTATAATGATTGACAAGATTAAAGCTTTTAAACGACAAGATTAAAGCTTTTAA
ACGAGGGTTTGTCAACTACTGTGTGAAAGTTCATTTGGGCATGAAAAAATTTCTGCTCTAAAGCAGCAACACTTTTTGACAACATTTATCAAGCAACACTTTTTGACAACatttatcaactactgtgtgaaagttcatttgggcatgaataagttttctgctccaaagcagcaacaccttttgacaacATTTATCAACTA
CTGTGTGAAAGTTCATTTCGGCATGAATAAGTTTCTGCTCCAAAGCAGCAACGGGCATGAATAAGTTCCTGCTCCAAAGCAACAATACTTTTGGACAACATTTAAATGTTTGCACTACCATACAGTGGGTGCTTTAAACTCTTTGAAAAATGCAAAGAGTAGGGCGTC

Intron 2 GTAAGTTCCT ... TCCAAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.32	181	72	71
A2	28.27	85	36	31
A3	26.37	134	46	50
A4	29.23	115	49	46
A5	20.43	37	8	11
A6	27.97	94	38	35
A7	23.66	271	88	80
A8	29.17	170	71	69
A9	29.41	150	66	59
A10	25.57	211	75	70

>TRINITY_DN48035_c0_g1_i3 from sample A7 / Example spliced isoform

AAAGTTCATTTGGGCATGAAAAGTTTTCTGCTCCAAAGCAACAACACTTTTTGACGACATTTATCAACTACTGTGTGAAAGTTCATTTGGGCATGAATAAGTTTTCTGCTCTAAAGCAGCAACACTTTTTGACAACATTTATCAACTACTGTGTGAAAGTTCATTTGGGCATGAATAAGTTCCTGCTCCAAAGCAACAAT
ACTTTTGGACAACATTTAAATGTTTGCAATACCATACAACAGGTACTTTGTAAAACGCAAAGAGTAGGGCGACATGGTATTCAAATTTTTTAGAGGAATGAAATCTCAACATCTTAAATGCTTTATAAAGCCGACAGAGTTGGCTTACATGGTAGTATCCAAAA

>TRINITY_DN48035_c0_g1_i4 from sample A7 / Example unspliced isoform:

TTGTATCTGTCATATTCACAACAATTTACTCTATACATGCACACATTGGATAATGTGTAGAAGGATAGCAACATTGGACATAACAAGAAGGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTTCAACACCATGTCATGAATAAGTACAGATTGTTGAATCTAATG
TGTAGAAGGATAGCAACATTGGACATAACAAGAAGGTTTTCACAACCCACGTTATAATGATTGACAAGATTAAAGCTTTTAAACGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTGTGTGAAAGTTCATTTGGGCATGAAAAGTTTTCTGCTCCAAAGCAACAACACTTTTTGACGACATTTATCAACTAC
TGTGTGAAAGTTCATTTGGGCATGAATAAGTTTTCTGCTCTAAAGCAGCAACACTTTTTGACAACATTTATCAAGCAACACTTTTTGACAACATTTATCAACTACTGTGTGAAAGTTCATTTGGGCATGAATAAGTTCCTGCTCCAAAGCAAgtaagttcctgctccaaagcagCAATACTTTTGGACAACATTTAAATG
TTTGCAATACCATACAACAGGTACTTTGTAAAACGCAAAGAGTAGGGCGACATGGTATTCAAATTTTTTAGAGGAATGAAATCTCAACATCTTAAATGCTTTATAAAGCCGACAGAGTTGGCTTACATGGTAGTATCCAAAAACTTTACAGGAATGAAATCTCAACATCTTCAATGTTTGCTTCATTTGGACCATCTTAA
ATGCTTCTGTACAAATAATATATTTGGGTTCCAAGTATCTAAAAGCTGAATGAAAGTGCTTTTGTACAAATAATATATTTGGGTTCCAAGTATCTAAA

Intron 3 GTCATGAATA ... TAACAAGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	88.45	29	213	231
A2	89.11	19	175	136
A3	89.68	21	192	173
A4	90.83	20	198	198
A5	92	6	69	69
A6	78.81	25	94	92
A7	88.46	34	277	244
A8	89.24	19	149	166
A9	89.45	22	193	180
A10	84.03	46	249	235

>TRINITY_DN109695_c1_g2_i6 from sample A4 / Example spliced isoform

AATGTGTAGAAGGATAGCAACATTGGACATAACAAGAAGGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTTCAACACCATGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTGTGTGAAAGTTCATTTG
GGCATGAAAAGTTTTCTGCTCCAAAGCAACAACACTTTTTGACGACATTTATCAACTACTGTGTGAAAGTTCATTTGGGCATGAATAAGTTTTCTGCTCCAAAGCAGCAACACTTTTTGACAACATTTATCAAGCAACACTTTTTGACAACATTTATCAACTACTGTGTGAAAGTTCATTTGGGCATGAGTAAGTTCCTG
CTCCAAAGCAGCAATACTTTTGGACAACATTTAAATGTTTGCAATACCATACAACGGGTGCTTTGTAAAACGCAAAGAGTAGGGCGACATGGTATTCAAATTTTTTAGAGGAATGAAATCTCAACATCTTGAATGCTTTATAAAGCCGACAGAGTTGGCTTACATGGTAGTATCCAAAAGCTTTACAGGAATGAAATCTC
AATATCTTCAATGTTTGCTTCATTTGGACCATCTTAAATGCTTTTGTACAAATAATATATTTGGGTTCCAAGTATCTAAAAGCTGAATGAAAGTGCTTTTGTACAAATAATATATTTGGGTTCCAAGTATCTAAAAGCT

>TRINITY_DN109695_c1_g2_i8 from sample A4 / Example unspliced isoform:

GATTCAACACCATGTCATGAATAATTACAGATTGCTGAATCTGTTGTATCTGTCATATTCACAACAATTTACTCTATACATGCACACATTGGATAATGTGTAGAAGGATAGCAACATTGGACATAACAAGAAGGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACT
TCAACACCATgtcatgaataagtacagattgttgaatctgttgtacctgtcatattcacagcaatttactctatacatgcacacattggataatgtgtagaaggatagcaacattggacataacaagaagGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTGTG
TG

Intron 4 GTCATGAATA ... TAACAAGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.67	29	175	202
A2	87.66	19	140	130
A3	87.5	21	138	156
A4	89.61	20	167	178
A5	91.37	6	55	72
A6	72.53	25	55	77
A7	85.56	34	199	204
A8	87.29	19	110	151
A9	85.08	22	109	142
A10	79.19	46	160	190

>TRINITY_DN109695_c1_g2_i6 from sample A4 / Example spliced isoform

AATGTGTAGAAGGATAGCAACATTGGACATAACAAGAAGGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTTCAACACCATGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTGTGTGAAAGTTCATTTG
GGCATGAAAAGTTTTCTGCTCCAAAGCAACAACACTTTTTGACGACATTTATCAACTACTGTGTGAAAGTTCATTTGGGCATGAATAAGTTTTCTGCTCCAAAGCAGCAACACTTTTTGACAACATTTATCAAGCAACACTTTTTGACAACATTTATCAACTACTGTGTGAAAGTTCATTTGGGCATGAGTAAGTTCCTG
CTCCAAAGCAGCAATACTTTTGGACAACATTTAAATGTTTGCAATACCATACAACGGGTGCTTTGTAAAACGCAAAGAGTAGGGCGACATGGTATTCAAATTTTTTAGAGGAATGAAATCTCAACATCTTGAATGCTTTATAAAGCCGACAGAGTTGGCTTACATGGTAGTATCCAAAAGCTTTACAGGAATGAAATCTC
AATATCTTCAATGTTTGCTTCATTTGGACCATCTTAAATGCTTTTGTACAAATAATATATTTGGGTTCCAAGTATCTAAAAGCTGAATGAAAGTGCTTTTGTACAAATAATATATTTGGGTTCCAAGTATCTAAAAGCT

>TRINITY_DN109695_c1_g2_i1 from sample A4 / Example unspliced isoform:

GATTCAACACCATGTCATGAATAATTACAGATTGCTGAATCTGTTGTATCTGTCATATTCACAACAATTTACTCTATACATGCACACATTGGATAATGTGTAGAAGGATAGCAACATTGGACATAACAAGAAGGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACT
TCAACACCATgtcatgaataagtacagattgttgaatctattcacagcaatttactctatacatgcacacattggataatgtgtagaaggatagcaacattggacataacaagaagGTCTTCACAACCCACGTTATAATGTTTGACAAGATTAAAGCTTTTAAACGAGGGTTTGTCAACTACTGTGTG