sp|P35685|RL7A1_ORYSJ ----> Large ribosomal subunit protein eL8z

Intron 1 GTGTGCGATT ... CTTCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20	96	0	24
A2	16	63	2	12
A3	18.69	87	0	20
A4	5.41	35	0	2
A5	23.68	29	0	9
A6	19.33	601	0	144
A7	19.88	548	0	136
A8	24	627	0	198
A9	23.32	490	0	149
A10	19.35	325	0	78

>TRINITY_DN111552_c0_g3_i1 from sample A2 / Example spliced isoform

AACAGCTCCGTCAAACAAACCTTATAGGATTTGGAAGGGCACTTGTTATATACCTTCAAACTTGATCGTGCCTGATCTTATTTGTAGTAGGTAGTGTAGCAGGTAGCAGTCTAGCAGGTAATCTGCTTATTTTTAGTACTTAGGTTCTGAGGTAGCAACCTAGAAGGTAAGCTAACAGAGCGAGGGAGCAGATCGATCGC
AGCAATGGCTCCGAAGAAAGGGGTTAAGGCTCCTGTTGCTGCTAAGAAGAAGGTGCCGAAGGTTGTTAATCCATTGTTTGAGAAGCGGCCCAAACAGTTTGGAATAGGGGGGTCTCTGCCGCCAAAGAGGGATTTGCACAGATTTGTGAAATGGCCGAAGGTTGTTCGCATCCAGAGGCAGCGGAGGGTATTGAAGCAGC
GTTTGAAGGTTCCTCCGGCTTTGAACCAATTCACCAAAACACTCGACAAGAATCTTGCGGCCAACCTTTTCAAGATGCTCCTAAAATACAGGCCAGAAGACAAAGCTGCAAAAAAGGAAAGGCTTCTGAAGAAAGCTCAGGCTGAGGCAGAGGGAAAAACTGTTGAAGTAAAGAAGCCTATTGTTGTGAAATATGGGATT
AATCATATAACTTACCTTATTGAGCAGAGTAAAGCTCAGTTGGTAATTATTGCACATGATGTTGATCCAATAGAGTTGGTGGTGTGGCTTCCTGCACTTTGTAGAAAAATGAACATCCCATATGCAATTGTCAAGGGGAAAGCACGTCTTGGAGCGATTGTGCACAAGAAAACAGCAACTGCTTTGTGTTTGACTTCTGT
GAAGAATGAGGACAAGATGGAATTCAGCAAGATTGTTGAGGCAGTAAAGGCCAACTTCAATGATAAGTTTGATGAGTATCGGCGCCAATGGGGAGGGGGCATCATGGGGACGAAATCTCAGGCAAAAACAAAGGCCAAGGAAAAAGTCATGGCAAAGGAAGCGGCACAAAGGCTCACATAGGAATAATTTTATGCAAGAT
TTTCCCCATCTTGAAGAGTTTGTTAGAGTATTTTTCGGAATATGTTGTGATTTTGATTTAGAGGTGTAGAGAAATATTGGATTTATGCTGAGAACGTTTTAAAGCTAACAATGGCTTTTTCCTCCAAGAAAGCAGCATGACTAATTGTCAATTCTGGGTAAAATGCTTAGCTAGCTTTCATTCATTGCAAA

>TRINITY_DN111552_c0_g3_i2 from sample A2 / Example unspliced isoform:

TTGTTGAGGCAGTAAAGgtgtgcgattaagatgttctgatttgtgctgttttgttcatcaagtaatgctatataaagtggaaaattaatgtcttaaataagtttaaactcttcttgcagGCCAACTTCAATGATAAGTTTGATGAGTATCGGCGCCAATGGGGAGGGGGCATCATGGGGACGAAATCTCAGGCAAAAACA
AAGGCCAAGGAAAAAGTCATGGCAAAGGAAGCGGCACAAAGGCTCACATAGGAATAATTTTATGCAAGATTTTCCCCATCTTGAAGAGTTTGTTAGAGTATTTTTCGGAATATGTTGTGATTTTGATTTAGAGGTGTAGAGAAATATTGGATTTATGCTGAGAACGTTTTAAAGCTAACAATGGCTTTTTCCTCCAAGAA
AGCAGCATGACTAATTGTCAATTCTGGGTAAAATGCTTAGCTAGCTTTCATTCATTGCAAA

Intron 2 GTTAGTAGAT ... TGCATGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.4	160	5	41
A2	14.91	194	0	34
A3	17.63	229	1	49
A4	21.19	119	2	32
A5	22.22	126	3	36
A6	17.99	465	0	102
A7	18.23	435	0	97
A8	16.52	293	0	58
A9	14.88	452	0	79
A10	12.18	339	0	47

>TRINITY_DN108268_c1_g1_i7 from sample A3 / Example spliced isoform

ATTTTTTTCAAGTGGGTTGCTTGACAACTTTGTCGTGTCGTAAGAGTTAACCCAGGGTCAGAGTAGTGACTCTGGAGTCAGAAGGGCAGTTAGCGTAGCGGGTAAAGGGTTTGTAGAATTAGGGTTTAGGAGAAGGGCTTTGTCTAAAGAATAAACAGGTTCAGTAGCTTGTTGTTGCCGTGCGCGTGGAAAGAGGTAGC
AGATCAGCTAGCAGAGCGAAGTAGAGCGAGGGAGCAGATCGATTCCAGCAATGGCTCCGAAGAGAGGGGTCAAGACACCGGTTGCTGCTAAGAAGAAGGTTCCGAAGGTTGTTAATCCATTGTTTGAGAAGCGACAGAAGCAGTTTGGGATTGGGGGAGCTCTGCCGCCCAAGAGGGATTTACACAGATTTGTGAAATGG
CCGAAGGTTGTTCGCATCCAGAGGCAGCGGAGGGTATTGAAGCAGCGTTTGAAGGTCCCTCCGGCCTTAAATCAGTTCACCAAGACCCTTGACAAGAATCTCGCTTCCAACCTTTTCAAGATGCTCCTTAAATACAGGCCAGAAGATAAAGCTGCAAAGAAGGAAAGACTTCTGAAGAAAGCTCAGTCTGAGGCAGAAGG
AAAGACTGTTGAAGTGAAGAAGCCTATTGTTGTGAAATATGGGATTAATCATATAACATACCTCATTGAGCAGAGTAAAGCTCAGTTGGTAGTTATTGCACATGATGTTGATCCAATAGAGTTGGTGGTGTGGCTTCCTGCACTTTGTAGAAAAATGAACATCCCATATGCTATTGTCAAGGGGAAAGCACGCCTTGGAG
CGATTGTGCACAAAAAGACAGCAACTGCCCTGTGCTTGACTTCTGTGAAAAATGAGGACAAGATGGAATTTAGCAAAATTGTTGAGGCAGTAAAGGCTAACTTCAATGACAAATTTGATGAATACCGGCGACAATGGGGAGGTGGCATTATGGGAACTAAATCCCAGGCCAAAACAAAGGCCAAGGAAAAACTCGTGGCC
AAGGAAGCAGCACAGAGGCTCAATTAAGATTAGTTCCTTGGGATATTTTTTCCATTTTGAAAATTTCATTGGATTGTTCTTTTTGCAATTTGTTGTAATTTGCTTAATAGGTATAGTAACATTAGAGTTTATACTAAGGAAGTTTTAAAGCTAACTTTAGCTTTGACCTCAGAAAACAGCAGGTCAATTTTGCAACTTGG
TATGTTGAGTAAATAACATTTTATACGCATTCATTGGCTTTATGCACTTTACCAACTTTTTGTATGCTGTGATGATGATATTTTCATTTTCTCCTTTTGTAGGTGTTGTTGATATTTATTGC

>TRINITY_DN108268_c1_g1_i10 from sample A3 / Example unspliced isoform:

CGGTTGCTGCTAAGAAGAAGgttagtagattcaatgcttctgtgtttctgggttatcgtgatcccttattattattgatagaagaatccatgaaatcatgtaccatgtattcattttagggtttgtatggatttggctgtataagctattgatttttagggtttgtattgatgcgggtgtgcatggcagGTTCCGAAGGT
TGTTAATCCATTGTTTGAGAAGCGACAGAAGCAGTTTGGGATTGGGGGAGCTCTGCCGCCCAAGAGGGATTTGCACAGATTTGTGAAATGGCCGAAGGTTGTTCGCATCCAGAGGCAGCGGAGGGTATTGAAGCAGCGTTTGAAGGTCCCTCCGGCCTTAAATCAGTTCACCAAGACCCTTGACAAGAATCTCGCTTCCA
ACCTTTTCAAGATGCTCCTTAAATACAGGCCAGAAGATAAAGCTGCAAAGAAGGAAAGACTTCTGAAGAAAGCTCAGTCTGAGGCAGAAGGAAAGACTGTTGAAGTGAAGAAGCCTATTGTTGTGAAATATGGGATTAATCATATAACATACCTCATTGAGCAGAGTAAAGCTCAGTTGGTAGTTATTGCACATGATGTT
GATCCAATAGAGTTGGTGGTGTGGCTTCCTGCACTTTGTAGAAAAATGAACATCCCATATGCTATTGTCAAGGGGAAAGCACGCCTTGGAGCGATTGTGCACAAAAAGACAGCAACTGCCCTGTGCTTGACTTCTGTGAAAAATGAGGACAAGATGGAATTTAGCAAAATTGTTGAGGCAGTAAAGGCTAACTTCAATGA
CAAATTTGATGAATACCGGCGACAATGGGGAGGTGGCATTATGGGAACTAAATCCCAGGCCAAAACAAAGGCCAAGGAAAAACTCGTGGCCAAGGAAGCAGCACAGAGGCTCAATTAAGATTAGTTCCTTGGGATATTTTTTCCATTTTGAAAATTTCATTGGATTGTTCTTTTTGCAATTTGTTGTAATTTGCTTAATA
GGTATAGTAACATTAGAGTTTATACTAAGGAAGTTTTAAAGCTAACTTTAGCTTTGACCTCAGAAAACAGCAGGTCAATTTTGCAACTTGGTATGTTGAGTAAATAACATTTTATACGCATTCATTGGCTTTATGCACTTTACCAACTTTTTGTATGCTGTGATGATGATATTTTCATTTTCTCCTTTTGTAGGTGTTGT
TGATATTTATTGC

Intron 3 GTATTGCAAC ... TTGCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.71	113	0	26
A2	20.97	98	0	26
A3	22.22	119	1	34
A4	18.43	62	0	14
A5	15.15	56	0	10
A6	18.9	562	0	131
A7	14.54	635	0	108
A8	18.43	425	0	96
A9	19.08	509	0	120
A10	16.26	345	0	67

>TRINITY_DN42377_c1_g1_i7 from sample A8 / Example spliced isoform

AAAAAAGGGACATTTTTTAAAGCCTGGAATTTTCAGTTTTGTAATATGGGAGTGTATTAAGCCTAGTAAATTGCTAGAACCTGGAAGAAACAAGTTTAAAATTTATATGAAATAATGTGGAATGTAAGCGTGGTCAAATGGGGATTTAAAGCAGTGCAAACTGCCTAACCACAAAAGGTTGTTTTCAAGCATTGGCTAGG
TTTGTTGAGTGGTCTCATCTTGATGGTGTCTCTTTTGTGTCTACGTTTCATTCATTATTAATGTGCATGTACTTCGGGTGCTTCAGTTTTATAATCTGTTTTAGGGGAGTAAAATATCACATTGGACAGTCGGAGGGGTTGGTTAATCAATTATTTCTTGGCTTCATTTAAAAGATGGTTGTAATCAATAACTTAATTTT
AACACATGCACATCAGCTTAATGCATGTTTGCATTATAGCATTTGGAAGTACAAAATCATCACTCTGCTTAGGTACAAAGTTTTGTGGGCACTGGTAATTGACAAAGAAATTGGACCAAGAGGAAACAATGAGGGAGAAAGGAAGGCTCAACTAGAGTTTGATAGTATAAATAAGGCCATTGTTTTGATCAATTCATTGT
AGTAGAGTGGGACATTGGTTGCTTGAAAGGTGAGGACAATGCTCATACAATCTAGGCAAGATTGAAGTCCATGTATGAGACTTCAAATTTCAGTGTTCTGCACTTTAAAAACCAACTGAATGGATTTAAGATGTCAGAAGGGAGTTTTATTTATGAATTAATCGTCTCAGTAGCAATGTTAGTTGTCACCTATCCAGAAC
TTGTTAGAGCAATCATATCATTCTAGGGTTTCAGATTGGTTTGTTGAGATCATGGGTTATAACATGTCTTTGATAAGGCTTTTGGCTCATACTCCATTTCTCTCTTTTAATGTAGCGGCCAACCTTTTCAAGATGCTCCTAAAATACAGGCCAGAAGACAAAGCTGCAAAAAAGGAAAGGCTTCTGAAGAAAGCTCAGGC
TGAGGCAGAGGGAAAAACTGTTGAAGTAAAGAAGCCTATTGTTGTGAAATATGGGATTAATCATATAACTTACCTTATTGAGCAGAGTAAAGCTCAGTTGGTAATTATTGCACATGATGTTGATCCAATAGAGTTGGTGGTGTGGCTTCCTGCACTTTGTAGAAAAATGAACATCCCATATGCAATTGTCAAGGGGAAAG
CACGTCTTGGAGCGATTGTGCACAAGAAAACAGCAACTGCTTTGTGTTTGACTTCTGTGAAGAATGAGGACAAGATGGAATTCAGCAAGATTGTTGAGGCAGTAAAGGCCAACTTCAATGATAAGTTTGATGAGTATCGGCGCCAATGGGGAGGGGGCATCATGGGGACGAAATCTCAGGCAAAAACAAAGGCCAAGGAA
AAAGTCATGGCAAAGGAAGCGGCACAAAGGCTCACATAGGAATAATTTTATGCAAGATTTTCCCCATCTTGAAGAGTTTGTTAGAGTATTTTTCGGAATATGTTGTGATTTTGATTTAGAGGTGTAGAGAAATATTGGATTTATGCTGAGAACGTTTTAAAGCTAACAATGGCTTTTTCCTCCAAGAAAGCAGCATGACT
AATTGTCAATTCTGGGTAAAATGCTTAGCTAGCTTTCATTCATTGCAATTTGTTTTACTTTCGTCTGATGTGATGTTTTTTGCATGAAAAAAATGTATTTTTCATTCCAG

>TRINITY_DN42377_c1_g1_i6 from sample A8 / Example unspliced isoform:

ATATAACTTACCTTATTGAGCAGgtattgcaacactaattgttcacatatctctaatttgcaatcttttttagatgtattgaaatattgagtctttactcttttatcttctatctgcaaatttgaagaatttatgtgttcttctcagttatgataatgaagatttggttgctttcagAGTAAAGCTCAGTTGGTAATTAT
TGCACATGATGTTGATCCAATAGAGTTGGTGGTGTGGCTTCCTGCACTTTGTAGAAAAATGAACATCCCATATGCAATTGTCAAGGGGAAAGCACGTCTTGGAGCGATTGTGCACAAGAAAACAGCAACTGCTTTGTGTTTGACTTCTGTGAAGAATGAGGACAAGATGGAATTCAGCAAGATTGTTGAGGCAGTAAAGG
CCAACTTCAATGATAAGTTTGATGAGTATCGGCGCCAATGGGGAGGGGGCATCATGGGGACGAAATCTCAGGCAAAAACAAAGGCCAAGGAAAAAGTCATGGCAAAGGAAGCGGCACAAAGGCTCACATAGGAATAATTTTATGCAAGATTTTCCCCATCTTGAAGAGTTTGTTAGAGTATTTTTCGGAATATGTTGTGA
TTTTGATTTAGAGGTGTAGAGAAATATTGGATTTATGCTGAGAACGTTTTAAAGCTAACAATGGCTTTTTCCTCCAAGAAAGCAGCATGACTAATTGTCAATTCTGGGTAAAATGCTTAGCTAGCTTTCATTCATTGCAATTTGTTTTACTTTCGTCTGATGTGATGTTTTTTGCATGAAAAAAATGTATTTTTCATTCC
AG