sp|Q8VYE2|TIM50_ARATH ----> Mitochondrial import inner membrane translocase subunit TIM50

Intron 1 GTAATCATAT ... TCCTTTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	6.1	185.7	2	12
A2	2.93	100	0	3
A3	8.7	168.3	0	16
A4	4.17	115	0	5
A5	0	36	1	0
A6	0.79	250.7	0	2
A7	0.21	466.3	0	1
A8	2.86	374.3	0	11
A9	0.5	403.7	0	2
A10	0	351.7	0	0

>TRINITY_DN132447_c1_g2_i3 from sample A1 / Example spliced isoform

GTAGATTTTCTTGTATGTGATGGTCCATGGAGATTTCCTTAGGGCAAACTGCCATAAGGTGAACACTCCTGCTATCGCGTAGGAATATTTTTCTGACTTCACGGCCGTCAAAACGACGTTAATCCGCGCTGAATTCTTGACACCAAATGCACATTTCCGTGAAGAACCAGCCACGACCTCACAGCCACGGTCTGCCTGAG
ACGACTGCAAGCATATATCCCCGACTTCATTTATGCCTTGATTTCTCCAAACCCTCGGAGCTTCAGTAGTCTGCATCAACTCCTCCGCCATTTACAGACATAAATTTGAATAGGGGCTTCAGATATGTCGGGCATTATGCGTTTCAAACGCCTGCTTCGCATCCCCGGCCAAGGCGTCACACCGAGGAGGTTTTCGGCGA
CTTCGGAGGCATCCGTCGTCTCCTCGGAAGCTAATCCTCGCCAACAAGCAAGTAGCGAAGGAGCAGAGCAGCAGGGCGACGGTAATGGTGGCGGATTTCAGAATAAAGAGAGATACGCGACCAGGTTTTTGAAGTTTTTGCTTGGCGGCACCTTTACTGCGGCTTCCGCAACGGCAGCTTATGCTTCTTACGCATATACA
ACAGAAGAAATAGAACAGATGACAGCATCTATGCGAAATTCAACTACTTACAGTGTACCTGATGATGCCTCTGCAGTAGATAGAGTTCGTGGCTTCCTTTACTCCACAACCATGGCAGTTCCAGCAAAAGCTACTGAACTATACTTGGATGCAAGGAGGACAATTGAAGACTATGTAAAGGGCTTTGCAGCACCGTCATC
TGAAAAGCTTCTTCCAGACTTGGCACCTCAAGAACAGCATGTTTTCACCCTTGTTCTTGATCTTAATGAAACCCTAATCTATTCAGATTGGAAGAGAGATCGAGGATGGAAAACATTTAAAAGACCTGGAGTAGAGGCATTTTTGGAGCATCTTGCACACTACTATGAAATTGTGGTTTATTCTGATCAATTGAATATGT
ATGTGGACCCAATAATGGAGAGGCTTGATCAAAAAGGCTTTGTTAGGTACAGGTTGTCACGGGACGCAACTCAGTATGTGAATGGAAAACATTATCGGGATTTGTCGAAGTTGAATAGAGATCCTGCAAAAGTGATTTATTTGAGTGGACATGCTTTGGAAACATGCCTGCAACCTGAGAATGCGTTGCCCATTAAGCCA
TGGAAGCTTGAATCAGATGATACAGCACTTTTAGATCTCATACCTTTTCTTGAGTATGTGGCACGCCATCACCCACATGACCTAAGGGAAGTTCTAACATCTTATCAAGGTGAAGACGTTGTAACTGAATTTCGTAAGCGTACCAAGGAGCATCAGAGACGACTTGAAGAACAGAAACAACAGGGTCGTTTTTGGCGCAG
AGGGAGTTGATTTGACCAGAAAAGTTGAAGATTCTTTTATATCAATCAAATATTGGCCACATTGATGTCTATCTGTCATTGAAGTTCTTGCAGATGTTAACTCATACCGTGTATATCCCTGCATATTAGTAGAAGCATTAGCGGAGGGAAGAAATCGTAAGCATGATGCTAAAATTCCAAGCTAATTTAGTAATTTTTGG
GGATGCTTTAATGATTGATTTGGTCCTGATGCAAGAAGTTCAGGCTTATATACATCTTTCAATTTTTTCTCGGCTAATAACTACAATCGAGTGTTGGAGCATTTTGTCCAGATTTTGGAGGGAAATCTATAGGGGTATCATAGAGGTACTGATTGATATTGAGCAGTTTTCTGATTTATTTAAAGCAGCCTTTCATGTAA
TAGTCATGTTTGATAAAATGTGTCCTTGGCTTCCATGGTCACTCCCTATTTGGGAGGTTGGTCATGCCAAGTGTGATCAATTTTGATACACTGTTATTGACTGGACATGATAAGTAAATCATTGTTGTGCAGATGAAAACTAGAAATCTAAGATAGCTTCCACATGTTGAAATAGTCAATAACTAAAATTTTGAAG

>TRINITY_DN132447_c1_g2_i1 from sample A1 / Example unspliced isoform:

TTTATTCTGATCAATTGAATATGgtaatcatattttaaatttgtcagatgttgcttggttgctgtaattattactgatttactagcctaaatattctgttattgttattttgctatatatttatttcaaggatgatataaaatataaccttgaattctcaactgataatgtttttgaccatttttcctttctagTATGTG

GACCCAATAATGGAGAGGCTTGATCAAAAAGGCTTTGTTAGGTACAGGTTGTCACGGGACGCAACTCAGTATGTGAATGGAAAACATTATCGGGTATGCCCTTCTGACATTTAAAGTATATGTTCACATATTAAATGATGTAATATGAAGATTCTTATCCTCAGAAAAGATGAAGTGCAGTCCTGGATTTATTTTCATCT
TTTTAATATTCTTCATGTTTTCTATTTCTTGGTTAGTTTAGTTTGAAACATAGAAAATAGAATATGTCAGGG

Intron 2 GTGAGCAAGT ... TATTTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.49	233	8	46
A2	14.37	149	5	25
A3	18.48	172	2	39
A4	14.11	140	9	23
A5	7.14	78	5	6
A6	14.41	285	2	48
A7	15.13	460	13	82
A8	15.46	350	6	64
A9	16.99	474	8	97
A10	18.11	416	4	92

>TRINITY_DN100699_c0_g3_i5 from sample A4 / Example spliced isoform

GTGCATGTAGATTTTCTTGTATGTGATGGTCCATGGAGATTTCCTTAGGGCAAACTGCCATAAGGTGAACACTCCTGCTATCGCGTAGGAATATTTTTCTGACTTCACGGCCGTCAAAACGACGTTAATCCGCGCTGAATTCTTGACACCAAATGCACATTTCCGTGAAGAACCAGCCACGACCTCACAGCCACGGTCTG
CCTGAGACGACTGCAAGCATATATCCCCGACTTCATTTATGCCTTGATTTCTCCAAACCCTCGGAGCTTCAGTAGTCTGCATCAACTCCTCCGCCATTTAAAGACATAAATTTGAATAGGGGCTTCAGATATGTCGGGCATTATGCGTTTCAAACGCCTGCTTCGCATCCCCGGCCAAGGCGTCACACCGAGGAGGTTTT
CGGCGACTTCGGAGGCATCCGTCGTCTCCTCGGAAGCTAATCCTCGCCAACAAGCAAGTAGCGAAGGAGCAGAGCAGCAGGGCGACGGTAATGGTGGCGGATTTCAGAATAAAGAGAGATACGCGACCAGGTTTTTGAAGTTTTTGCTTGGCGGCACCTTTACTGCGGCTTCCGCAACGGCAGCTTATGCTTCTTACGCA
TATACAACAGAAGAAATAGAACAGATGACAGCATCTATGCGAAATTCAACTACTTACAGTGTACCTGATGATGCCTCTGCAGTAGATAGAGTTCGTGGCTTCCTTTACTCCACAACCATGGCAGTTCCAGCAAAAGCTACTGAACTATACTTGGATGCAAGGAGGACAATTGAAGACTATGTAAAGGGCTTTGCAGCACC
GTCATCTGAAAAGCTTCTTCCAGACTTGGCACCTCAAGAACAGCATGTTTTCACCCTTGTTCTTGATCTTAATGAAACCCTAATCTATTCAGATTGGAAGAGAGATCGAGGATGGAAAACATTTAAAAGACCTGGAGTAGAGGCATTTTTGGAGCATCTTGCACACTACTATGAAATTGTGGTTTATTCTGATCAATTGA
ATATGTATGTGGACCCAATAATGGAGAGGCTTGATCAAAAAGGCTTTGTTAGGTACAGGTTGTCACGGGACGCAACTCAGTATGTGAATGGAAAACATTATCGGGATTTGTCGAAGTTGAATAGAGATCCTGCAAAAGTGATTTATTTGAGTGGACATGCTTTGGAAACATGCCTGCAACCTGAGAATGCGTTGCCCATT
AAGCCATGGAAGCTTGAATCAGATGATACAGCACTTTTAGATCTCATACCTTTTCTTGAGTATGTGGCACGCCATCACCCACATGACCTAAGGGAAGTTCTAACATCTTATCAAGGTGAAGACGTTGTAACTGAATTTCGTAAGCGTACCAAGGAGCATCAGAGACGACTTGAAGAACAGAAACAACAGGGTCGTTTTTG
GCGCAGAGGGAGTTGATTTGACCAGAAAAGTTGAAGATTCTTTTATATCAATCAAATATTGGCCACATTGATGTCTATCTGTCATTGAAGTTCTTGCAGATGTTAACTCATACCGTGTATATCCCTGCATATTAGTAGAAGCATTAGCGGAGGGAAGAAATCATAAGCATGATGCTAAAATTCCAAGCTAATTTAGTAAT
TTTTGGGGATGCTTTAATGATTGATTTGGTCCTGATGCAAGAAGTTCAGGCTTATATACATCTTTCAATTTTTTCTCGGCTAATAACTACAATCGAGTGTTGGAGCATTTTGTCTAGATTTTGGAGGGAAATCTACAGGGGTATCATAGAGGTACTGATTGATATTGAGCAGTTTTCTGATTTATTTAAAGCAGCCTTTC
ATGTAATAGTCGTGTTTGATAAAATGTGTCCTTGGCTTCCATGGTCACTCCCTATTTGGGAGGTTGGTCATGCCAAGTGTGATCAATTTTGATACACTGTTATTGACTGGACATGATAAGTAAATCATTGTTGTGCAGATGAAAACTAGAAATCTAAGATAGCTTCCACATGTTGAAATAGTCAATAACTAAAATTTTGA
AGGAATATAAATTATACCAGGG

>TRINITY_DN100699_c0_g3_i7 from sample A4 / Example unspliced isoform:

GTTGCTTAAAATATGATAGATCTTCAGCTTTTCCCAAAATAGACTGCCCAATCTTAAAATAACATTAATTGCTTTTCTTGCATGTGCATGACTTTTCTACTCTTGGTGCATGTACGTTTTTGACATTTCTACTATTGTATCATTGAAAAGTCCTATCTCTATGTAAATGAATATGAAAATTTCTAACACAATTGATAGAA
ACCAAGAGAGAGGGTGATACTTTAGCTCTTGAGAAATTGCAATGAATTAGCATTTGATATTGTTGTGTATTGCAGCCAGGATGTCTTGATGCCTTTTTGTATATGTACTCTTTTGATAAGAAATAAACTTTTGCAGCAAAAATCATTGTCTATGGATATAAAGATCCATTCATATATTTTGTTGAAGAAGTAGCATTGTG
GTTAGTTCTTAATTTATGAAAGATGATCTATGTACCAAGGACAGTTTTTGACTTTTGTCTTGTTGGATATTGCATTGCAGCATATACAACAGAAGAAATAGAACAGATGACAGCATCTATGCGAAATTCAACTACTTACAGTGTACCTGATGATGCCTCTGCAGTAGATgtgagcaagtggcagtgtgcctttagatgag
atattttattccatcgccctgttatcggaaacataaaaattcaccactgaatttacttaccaaggatgatccattagtcgggataaactgtaaaccattatatcataatgaatcataatgaacatatatcttggcagtcgtgtatatatatatatatatatatatatatatatgggaaagtacaagtttgaacatcactt
gaaatgaatctaaaaatgttgatattcttaagaacattctgaggcaatgtttacattgcttttaatggtctttctctgtttcatttatttgatttatttgtcaggggaagttagtattttggtaatttcacacatatccattattataaatatagttataacataaatgtgttgttatctttgtatttatcagAGAGTTC
GTGGCTTCCTTTACTCCACAACCATGGCAGTTCCAGCAAAAGCTACTGAACTATACTTGGATGCAAGGAGGACAATTGAAGACTATGTAAAGGGCTTTGCAGCACCGTCATCTGAAAAGCTTCTTCCAGACTTGGCACCTCAAGAACAGCATGTTTTCACCCTTGTTCTTGATCTTAATGAAACCCTAATCTATTCAGAT
TGGAAGAGAGATCGAGGATGGAAAACATTTAAAAGACCTGGAGTAGAGGCATTTTTGGAGCATCTTGCACACTACTATGAAATTGTGGTTTATTCTGATCAATTGAATAT

Intron 3 GTGAGCAAGT ... TATTTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.39	233	59	46
A2	17.91	149	40	25
A3	17.11	172	32	39
A4	17.65	140	37	23
A5	15.22	78	22	6
A6	15.3	285	55	48
A7	16.97	460	106	82
A8	17.84	350	88	64
A9	17.64	474	106	97
A10	16.72	416	75	92

>TRINITY_DN105302_c1_g1_i5 from sample A5 / Example spliced isoform

TGTAGATTTTCTTGTATGTGATGGTCCATGGAGATTTCCTTAGGGCAAACTGCCATAAGGTGAACACTCCTGCTATCGCGTAGGAATATTTTTCTGACTTCACGGCCGTCAAAACGACGTTAATCCGCGCTGAATTCTTGACACCAAATGCACATTTCCGTGAAGAACCAGCCACGACCTCACAGCCACGGTCTGCCTGA
GACGACTGCAAGCAAATATCCCCGACTTCATCAATGCCTTGATTTCTCCAAACCCTCGGAGCTTCAGTAGTCTGCATCAACTCCTCCGCCATTTACAGACATAAATTTGAATAGGGGCTTCAGATATGTCGGGCATTATGCGTTTCAAACGCCTGCTTCGCATCCCCGGCCAAGGCGTCACACCGAGGAGGTTTTCGGCG
ACTTCGGAGGCATCCGTCGTCTCCTCGGAAGCTAATCCTCGCCAACAAGCAAGTAGCGAAGGAGCAGAGCAGCAGGGCGACGGTAATGGTGGCGGATTTCAGAATAAAGAGAGATACGCGACCAGGTTTTTGAAGTTTTTGCTTGGCGGCACCTTTACTGCGGCTTCCGCAACGGCAGCTTATGCTTCTTACGCATATAC
AACAGAAGAAATAGAACAGATGACAGCATCTATGCGAAATTCAACTACTTACAGTGTACCTGATGATGCCTCTGCAGTAGATAGAGTTCGTGGCTTCCTTTACTCCACAACCATGGCAGTTCCAGCAAAAGCTACTGAACTATACTTGGATGCAAGGAGGACAATTGAAGACTATGTAAAGGGCTTTGCAGCACCGTCAT
CTGAAAAGCTTCTTCCAGACTTGGCACCTCAAGAACAGCATGTTTTCACCCTTGTTCTTGATCTTAATGAAACCCTAATCTATTCAGATTGGAAGATTTTGACTGGGGAATCCATGGACAATCAAAGCTCAACAATCGCTTATGTTCTCAGTGTTGGAGAATGTGCAGTTTTATTGGTTATCAAGAAACAACATGCTGCT
TCACTTTCTTCTACCATGCCAGAGTGCAGGGCTGCAATAAAAGGGGCATGTGAGGCAGTTTGGCTTAGGAGAATACTTGTAGATTTACTGATTAAACGAAGTGTTTGTTTTTTCCACATATGTTGTGGGTCTGGAAATAGTAATATTCTGCTATGAAGAGGTCTACCTTCCATTGAATTAAATAGTCTATGTTTTGGAAA
ATACTCTCCTTTTAGAATTAGGGTATGTGATCCAAACATTTTCTATGAGCCATAAATTGTTGGGTATGCTGTAAAGTGGTTCATATGTTGATTTAATATCTATGGTTGAACATTGGAATAATTGCATATTTTAAATTTGCTGCAAATGCCTGGTGGTTGTCATTTGATTTTTGTCACTAAATGTTTCTTGTTGAATGTAG
TTGAAATTAATGACTAGTCACTAAA

>TRINITY_DN105302_c1_g1_i3 from sample A5 / Example unspliced isoform:

TGTAGATTTTCTTGTATGTGATGGTCCATGGAGATTTCCTTAGGGCAAACTGCCATAAGGTGAACACTCCTGCTATCGCGTAGGAATATTTTTCTGACTTCACGGCCGTCAAAACGACGTTAATCCGCGCTGAATTCTTGACACCAAATGCACATTTCCGTGAAGAACCAGCCACGACCTCACAGCCACGGTCTGCCTGA
GACGACTGCAAGCAAATATCCCCGACTTCATCAATGCCTTGATTTCTCCAAACCCTCGGAGCTTCAGTAGTCTGCATCAACTCCTCCGCCATTTACAGACATAAATTTGAATAGGGGCTTCAGATATGTCGGGCATTATGCGTTTCAAACGCCTGCTTCGCATCCCCGGCCAAGGCGTCACACCGAGGAGGTTTTCGGCG
ACTTCGGAGGCATCCGTCGTCTCCTCGGAAGCTAATCCTCGCCAACAAGCAAGTAGCGAAGGAGCAGAGCAGCAGGGCGACGGTAATGGTGGCGGATTTCAGAATAAAGAGAGATACGCGACCAGGTTTTTGAAGTTTTTGCTTGGCGGCACCTTTACTGCGGCTTCCGCAACGGCAGCTTATGCTTCTTACGCATATAC
AACAGAAGAAATAGAACAGATGACAGCATCTATGCGAAATTCAACTACTTACAGTGTACCTGATGATGCCTCTGCAGTAGATgtgagcaagtggcagtgtgcctttagatgagatattttattccatcgccctgttatcggaaacataaaaattcaccactgaatttacttaccaaggatgatccattagtcgggataaa
ctgtaaaccattatatcataatgaatcataatgaacatatatcttggcagtcgtgtatatatatatatgggaaagtacaagtttgaacatcacttgaaatgaatctaaaaatgttgatattcttaagaacattctgaggcaatgtttacattgcttttaatggtctttctctgtttcatttatttgatttatttgtcagg
ggaagttagtattttggtaatttcacacatatccattattataaatatagttatgacataaatgtgttgttatctttgtatttatcagAGAGTTCGTGGCTTCCTTTACTCCACAACCATGGCAGTTCCAGCAAAAGCTACTGAACTATACTTGGATGCAAGGAGGACAATTGAAGACTATGTAAAGGGCTTTGCAGCAC
CGTCATCTGAAAAGCTTCTTCCAGACTTGGCACCTCAAGAACAGCATGTTTTCACCCTTGTTCTTGATCTTAATGAAACCCTAATCTATTCAGATTGGAAGAGAGATCGAGGATGGAAAACATTTAAAAGACCTGGAGTAGAGGCATTTTTGGAGCATCTTGCACACTACTATGAAATTGTGGTTTATTCTGATCAATTG
AATATGTATGTGGACCCAATAATGGAGAGGCTTGATCAAAAAGGCTTTGTTAGGTACAGGTTGTCACGGGACGCAACTCAGTATGTGAATGGAAAACATTATCGGGATTTGTCGAAGTTGAATAGAGATCCTGCAAAAGTGATTTATTTGAGTGGACATGCTTTGGAAACATGCCTGCAACCTGAGAATGCGTTGCCCAT
TAAACCATGGAAGCTTGAACCAGATGATACAGCACTTTTAGATCTCATACCTTTTCTTGAGTATGTGGCACGCCATCACCCACATGACCTAAGGGAAGTTCTAACATCTTATCAAGGTGAAGACGTTGTAACTGAATTTCGTAAGCGTACCAAGGAGCATCAGAGACGACTTGAAGAACAGAAACAACAGGGTCGTTTTT
GGCGCAGAGGGAGTTGATTTGACCAGAAAAGTTGAAGATTCTTTTATATCAATCAAATATTGGCCACATTGATGTCTATCTGTCATTGAAGTTCTTGCAGATGTTAACTCATACCATGTATATCCCTGCATATTAGTAGAAGCATTAGCGGAGGGAAGAAATCATAAGCATGATGCTAAAATTCCAAGCTAATTTAGTAA
TTTTTGGGGATGCTTTAATGATTGATTTGGTCCTGATGCAAGAAGTTCAGGCTTATATACATCTTTCAATTTTTTCTCGGCTAATAACTACAATCGAGTGTTGGAGCATTTTGTCTAGATTTTGGAGGGAAATCTACAGGGGTATCATAGAGGTACTGATTGATATTGAGCAGTTTTCTGATTTATTTAAAGCAGCCTTT
CATGTAATAGTCATGTTTGATAAAATGTGTCCTTGGCTTCCATGGTCACTCCCTATTTGGGAGGTTGGTCATGCCAAGTGTGATCAATTTTGATACACTGTTATTGACTGGACATGATAAGTAAATCATTGTTGTGCAGATGAAAACTAGAAATCTAAGATAGCTTCCACATGTTGAAATAGTCAATAACTAAAATTTTG
AAGGAATATAAATTATATCAGGG