NA ----> NA

Intron 1 GCGAGAGTGA ... CATTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	85.22	15	28	145
A2	81.16	13	37	75
A3	72.55	21	32	79
A4	70.86	22	29	78
A5	94.12	2	17	47
A6	95.22	6	66	173
A7	92.54	17	121	301
A8	90.2	22	103	302
A9	82.47	27	56	198
A10	90.06	16	64	226

>TRINITY_DN111562_c0_g1_i6 from sample A4 / Example spliced isoform

TTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAACACTTTAAAAAAAGTCTTCCATCATTTGAGTGGGAGTTTAGGTTGTCACGTTGGCAATTGTTTGCAATTTCTTTGGACAGACTTCTGAATGACCCCAAGTATGATTTTCTCCTATGCCCCTTCAGAGCGGC
TTTTAGTTAGCTGCATTGCCATATATGTGTCCCGTTTTGGTATTTCTTCAAGTGAGCGTGCATGCGTCTCTTTTCTGTTGCGTCTCTTTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAGCACCTTACAAAAAGTCTTCTTCAACCTGCAGCAATAAATTGAAT
GAGTTGGCATTATTTTTTCATCTGGCTTCCTCTTGTGGCATTGTCTCTGGATATGGCATTCAATACTCCAGAGTGGCTTTTCTGATCATTCTGCAGGCGAGCGTGAGCAGATGATACCAGACGTGCATTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAACATGAATACTGCAGGACTGCTTAGTAGATATAT
CATAATGACTTCTTCACTCTGCTGCAATGAATTGAATGAGTCGGTGAGAGTGAGAAGATGATTCATGATACCAGAGCTGCATTACTATCGGATTGCGGAGCATTTTATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAATATCTTTCTCACCCTAGCTTCACCCTAGCTGCAATGAAGTGGTGCAGGTGGGGTTCTTA
CATTAGACCATAGACCATGGTAAATACTTTAAACTATGGATAAATTTGGGCTCGGGGACTAATACCAAAAGTGAATTCTGTGTATTTTGGTTTATCCTTTATTTTGCTAGTATTCGTCTTTTGGAGCTGAGGCAGATTACGGGTGACTCCCAGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGT
GCTCTCTCCTTGGCTTGACAAGATCAGAT

>TRINITY_DN111562_c0_g1_i13 from sample A4 / Example unspliced isoform:

CCAAATCTACAACAGCTGTAGCAGTATTAATTGAGGTGTCAAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTAGGTTGTCACGTTGGCAATTGTTTGCAATTTCTTTGGGCAGACTTCAGAATGACCCCAGGTATGATTTTCTCCTATGCCCCTTCAGAGCGGCTTTTAGTTAGCTGCATTGCCATGGC
CATATGTGTCCCGTTTTGGTATTTCTTCAAGTGAGCGTGCATGCGTCTCTTTTCTGTTGCGTCTCTTTTCTGGTCTTACTTCAGgcgagagtgagaagatggttggatgggtgcatttactatcagattggagagcactttacaaaaagccttcctcaacctgctgcaatgaattgaatcagttggcattatttttacat
ctggcttcctctgtggcattgtctttggatatggccttcgatactccagagtggcttttacgtgagaagatggttgatcattctgcagGCGAGCGTGAGAAGATGATACCAGACGTGCATTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAACATGAATACTGCAGGACTGCTTAGTAGATATATCATAATGA
CTTCTTCACTCTGCTGCAATGAATTGAATGAGTCGGTATTTTTTTAACCTCTGGCTTCTTGTGGCGTGTACTATGGTCTTCATTTTCTCTGGATATATTTTTCGAAACTCCAGAGTGGCTTTCTGGTCTTCCTTCAGGTGAGAGTGAAAAGATGATTC

Intron 2 GTATGATTTT ... CTTACTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.55	18	129	261
A2	91.38	15	69	249
A3	91.62	14	90	216
A4	85.96	25	110	196
A5	90	7	35	91
A6	91.3	24	133	371
A7	90.15	48	218	661
A8	96.56	15	219	624
A9	86	46	127	438
A10	90.11	35	163	475

>TRINITY_DN44034_c0_g1_i7 from sample A9 / Example spliced isoform

GACTGCAAACAGAAGAAACTATAAATTCCTGTATCGGTGTACTGTGGTAGTGTGGTTTACAATAGACTGCAAACAGAAGAAACTATATATGTATATGTTGGTTGTGTGAAGTAGAGGCCAAATCGACAACAGATGTTGCAGTATTAATTGAGGTGTCAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTA
TGTGGTCACGTTGGCAATTGGTTGTCACGTTGGCAATTATTTGCAATTTCTTTGGACAGACTTCAGAATGACCCCAAGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTG

>TRINITY_DN44034_c0_g1_i3 from sample A9 / Example unspliced isoform:

GCAGTATTAATTGAGGTGTCAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTATGTGGTCACGTTGGCAATTGGTTGTCACGTTGGCAATTATTTGCAATTTCTTTGGACAGACTTCAGAATGACCCCAAgtatgattttctcctattccccttcagagcggcttttagttaactgcatggccatatgtg
tcccgttttggtatttcttcaagtgagcgtgcgtctcttttctcttttctggtcttacttcagGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGTAGAGCACTTTACAAAAAGTCTTCCTCAACCTGCTGCAATGAATTGAATGAGTTGGCATTATTTTTACATCTGGCTTCCAATTGTGGCATT
GTCTTTGGATATGGCCTTCAATACTCCAGAGTGGCTTTTATGATCATTCTGCAGGCGAGCGTGAGAAGATGATACCAGACGTGCGTTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAATATGAATACTGCAGGACTGCTTAGTAGATATATCATAATGACTTCTT

Intron 3 GCGAGCGTGA ... CATTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	95.4	12.3	221.3	297
A2	95.39	9	202	176.7
A3	94.15	11.7	185.3	194
A4	92.06	14.3	155.7	183
A5	97.02	2.3	74	81
A6	98.62	5	310.3	414
A7	97.77	13.7	572.7	638.3
A8	97.56	15	556	662
A9	95.37	20.7	370.7	485
A10	97.51	11.3	394.3	505

>TRINITY_DN111562_c0_g1_i20 from sample A4 / Example spliced isoform

TTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAACACTTTAAAAAAAGTCTTCCATCATTTGAGTGGGAGTTTAGGTTGTCACGTTGGCAATTATTTGCAATTTCTTTGGACAGACTTCAGAATGACCCCAAGATATGTGTCCCGTTTTGGTATTTTTCTTCAAG
TGAGCTGCGTCTCTTTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAGCACCTTACAAAAAGTCTTCTTCAACCTGCAGCAATAAATTGAATGAGTTGGCATTATTTTTTCATCTGGCTTCCTCTTGTGGCATTGTCTCTGGATATGGCATTCAATACTCCAGAG
TGGCTTTTCTGATCATTCTGCAGGCGAGCGTGAGCAGATGATACCAGACGTGCATTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAACATGAATACTGCAGGACTGCTTAGTAGATATATCATAATGACTTCTTCACTCTGCTGCAATGAATTGAATGAGTCGGTGAGAGTGAGAAGATGATTCATGATACCA
GAGCTGCATTACTATCGGATTGCGGAGCATTTTATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAATATCTTTCTCACCCTAGCTTCACCCTAGCTGCAATGAAGTGGTGCAGGTGGGGTTCTTACATTAGACCATAGACCATGGTAAATACTTTAAACTATGGATAAATTTGGGCTCGGGGACTAATACCAAAAGTG
AATTCTGTGTATTTTGGTTTATCCTTTATTTTGCTAGTATTCGTCTTTTGGAGCTGAGGCAGATTACGGGTGACTCCCAGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGAT

>TRINITY_DN111562_c0_g1_i19 from sample A4 / Example unspliced isoform:

TTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAACACTTTAAAAAAAGTCTTCCATCATTTGAGTGGGAGTTTAGGTTGTCACGTTGGCAATTATATGTGTCCCGTTTTGGTATTTTTCTTCAAGTGAGCTGCGTCTCTTTTCTGGTCTTACTTCAGgcgagcgt
gagaagatggttggataggtgcatttactatcagattgcagagcactttacaaaaagtcttcctcaacctctgcaataaattcaaatgagttggcattattttttcatctggcttcctcttgtctctggatatggcattcaatactccagagtggcttttctgatcattctgcagGCGAGCGTGAGAAGATGATACCAGA
CGTACATTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAACATGAATACTGCAGGACTGCTTAGTAGATATATCATAATGACTTCTTCACCCTGCTGCAATGAATTGAATGAGTTGGGTCTTCCTTCAGGTGAGAGTGAGAAGATGATTCATGATACCAGAGCTGCATTACTATCGGATTGCGGAGCATTTTAT
CTAAAGCTTCCTATGTGTTTAGATTTATTAAAATATCTTTCTCACCCTAGCTGCAATGAAGTAGATGAGTTGGTATTTGTTTTGACTTAACTACGTCTTCACCGAATGGCACGCTTCCTCTTCCTCTTCGGTGCCTCTCGACATGTTCTTCAATACTTCAGCGTGGCCTTCACTGATTAGATTTGGTATTCATTCAAGTG
AGCGTGTTGGTCTTGTATTTGTTTTTTAGATGAATGGTATGGCCATTTAAGATGGACTTTTGTGCAATATGTGGTCCGCCTTGGTATTCTTTCAAACGAGCGTGTTCGTCTTCTGTATGGTCTTCCTACAGGCGAGCGCGTGAATATTATAAGGGTTGCTTAATACTATTAAAATTGCAAGGCACTTTGAGGAATAACAG
CATCAATTTGCAGAAATGCTTTTACCTCAACTAAGCAAAGCTTACCATGATGTCAGCAGATATATCAAATTCTCACTCATTCTATAGGTGTAATGAAATGTTTTGAGATCTGCCTCTTCCCATGATTTCAGATTTGCTATAGACTTGCAGGTTATTATGGTATTTTGATTAAGAACTCAGTTGTAGAAATGTATAATTAT
GGGCCATTTTATGTCAATATGACCCATCTTTGTGGACTTTTTCAGTCTAGCGCCCTTTTCTACAGGGCGGCGTGACTTTCTTCCCAGCTTTACTAAAATATCAGGTGGAACGGTATAGCAGATTTTCCAGCCCAAGTACTATAATGAATTGGGATCTTTTCTCCTTCCCAATGCGTATGTGATGAATTGGCATTTCTTGA
CCTTGCTATTGGGCT

Intron 4 GTATGCGTAT ... TTTTGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	92.26	13	155	40
A2	83.48	19	96	32
A3	88.46	12	92	30
A4	83.67	16	82	26
A5	83.64	9	46	21
A6	84.96	20	113	28
A7	80.36	55	225	87
A8	86.85	33	218	42
A9	81.99	38	173	40
A10	81.76	29	130	56

>TRINITY_DN44034_c0_g1_i13 from sample A9 / Example spliced isoform

GCCGTTATCAGATGGTACACCCAGCTCTCAAATTCCTGTAATCGGTGTACTGTGGTTTACAATAGACTTCAAACAGAAGAAACTATATATGTATATGTTGGTTCTGTGTGAAGTAGAGACTTTGGTCCTGAGCCGCCTTTAAAAGCTCTGCCCAGAGAGATCAATTATTTATTGGGCCAAGAATGATTTTCTTCTATGCC
TCTTCAGAGCTGCTTTAGTTAGCTGCATGGCCATATGTGTCCCGTTTTGGTATTTCTTCAAGTGAGCGTGCGTCTCTTTTCTCTTTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGTAGAGCACTTTACAAAAAGTCTTCCTCAACCTGCTGCAATGAATTGAATGAG
TTGGCATTATTTTTACATCTGGCTTCCAATTGTGGCATTGTCTTTGGATATGGCCTTCAATACTCCAGAGTGGCTTTTATGATCATTCTGCAGGCGAGCGTGAGAAGATGATACCAGACGTGCGTTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAATATGAATACTGCAGGACTGCTTAGTAGATATATCAT
AATGACTTCTT

>TRINITY_DN44034_c0_g1_i6 from sample A9 / Example unspliced isoform:

GCCGTTATCAGATGGTACACCCAGCTCTCAAATTCCTGTAATCGGTGTACTGTGGTTTACAATAGACTTCAAACAGAAGAAACTATATATGTATATGTTGGTTCTGTGTGAAGTAGAGgtatgcgtattgtactttttaatcacatttgtaggtatcatggcatttgtcagatttccaattccctatatagatcctttgc
ttctgaatcgtcttaattgtgtattcaggcatgcatattttgggttgtattttgtgaaagtccaaatttacaacagttgtagcagcattaattgaggtgtcaatgaaattgcataatattcagcatcatttgagtgggagtttatgtggtcacgttggcaattggttgtcacgttggcaattttttgcaatttttttgga
cagACTTTGGTCCTGAGCCGCCTTTAAAAGCTCTGCCCAGAGAGATCAATTATTTATTGGGCCAAGAATGATTTTCTTCTATGCCTCTTCAGAGCTGCTTTAGTTAGCTGCATGGCCATATGTGTCCCGTTTTGGTATTTCTTCAAGTGAGCGTGCGTCTCTTTTCTCTTTTCTGGTCTTACTTCAGGCGAGCGTGAGAA
GATGGTTGGATAGGTGCATTTACTATCAGATTGTAGAGCACTTTACAAAAAGTCTTCCTCAACCTGCTGCAATGAATTGAATGAGTTGGCATTATTTTTACATCTGGCTTCCAATTGTGGCATTGTCTTTGGATATGGCCTTCAATACTCCAGAGTGGCTTTTATGATCATTCTGCAGGCGAGCGTGAGAAGATGATACC
AGACGTGCGTTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAATATGAATACTGCAGGACTGCTTAGTAGATATATCATAATGACTTCTT

Intron 5 GTGGGGTTCT ... ATTTTGCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.47	24	69	22
A2	58.62	24	43	25
A3	63.64	24	57	27
A4	66.22	25	66	32
A5	66.67	6	14	10
A6	69.15	29	79	51
A7	69.33	46	112	96
A8	71.68	48	134	109
A9	70.31	34	90	71
A10	59.83	47	95	45

>TRINITY_DN111562_c0_g1_i14 from sample A4 / Example spliced isoform

TTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAACACTTTAAAAAAAGTCTTCCATCATTTGAGTGGGAGTTTAGGTTGTCACGTTGGCAATTGTTTGCAATTTCTTTGGACAGACTTCTGAATGACCCCAAGTATGATTTTCTCCTATGCCCCTTCAGAGCGGC
TTTTAGTTAGCTGCATTGCCATATATGTGTCCCGTTTTGGTATTTCTTCAAGTGAGCGTGCATGCGTCTCTTTTCTGTTGCGTCTCTTTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAGCACCTTACAAAAAGTCTTCTTCAACCTGCAGCAATAAATTGAAT
GAGTTGGCATTATTTTTTCATCTGGCTTCCTCTTGTGGCATTGTCTCTGGATATGGCATTCAATACTCCAGAGTGGCTTTTCTGATCATTCTGCAGGCGAGCGTGAGCAGATGATACCAGACGTGCATTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAACATGAATACTGCAGGACTGCTTAGTAGATATAT
CATAATGACTTCTTCACTCTGCTGCAATGAATTGAATGAGTCGGTGAGAGTGAGAAGATGATTCATGATACCAGAGCTGCATTACTATCGGATTGCGGAGCATTTTATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAATATCTTTCTCACCCTAGCTTCACCCTAGCTGCAATGAAGTGGTGCAGTATTCGTCTTTT
GGAGCTGAGGCAGATTACGGGTGACTCCCAGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGAT

>TRINITY_DN111562_c0_g1_i20 from sample A4 / Example unspliced isoform:

TTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAACACTTTAAAAAAAGTCTTCCATCATTTGAGTGGGAGTTTAGGTTGTCACGTTGGCAATTATTTGCAATTTCTTTGGACAGACTTCAGAATGACCCCAAGATATGTGTCCCGTTTTGGTATTTTTCTTCAAG
TGAGCTGCGTCTCTTTTCTGGTCTTACTTCAGGCGAGCGTGAGAAGATGGTTGGATAGGTGCATTTACTATCAGATTGCAGAGCACCTTACAAAAAGTCTTCTTCAACCTGCAGCAATAAATTGAATGAGTTGGCATTATTTTTTCATCTGGCTTCCTCTTGTGGCATTGTCTCTGGATATGGCATTCAATACTCCAGAG
TGGCTTTTCTGATCATTCTGCAGGCGAGCGTGAGCAGATGATACCAGACGTGCATTACTATCAGATAGTAGAGCATTACAATGAGGAAAAATAACATGAATACTGCAGGACTGCTTAGTAGATATATCATAATGACTTCTTCACTCTGCTGCAATGAATTGAATGAGTCGGTGAGAGTGAGAAGATGATTCATGATACCA
GAGCTGCATTACTATCGGATTGCGGAGCATTTTATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAATATCTTTCTCACCCTAGCTTCACCCTAGCTGCAATGAAGTGGTGCAGgtggggttcttacattagaccatagaccatggtaaatactttaaactatggataaatttgggctcggggactaataccaaaagtg
aattctgtgtattttggtttatcctttattttgctagTATTCGTCTTTTGGAGCTGAGGCAGATTACGGGTGACTCCCAGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGAT