NA ----> NA

Intron 1 GTGGGGAGGC ... TGTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.25	29	214	399.3
A2	87.6	28	122.7	276
A3	90.39	22.3	156	266
A4	85.23	25	86.7	202.3
A5	85.57	21.7	81	176
A6	90.81	1.3	5	21.3
A7	100	0	7.3	21
A8	100	0	4.3	13.7
A9	100	0	5	19
A10	100	0	4	10

>TRINITY_DN111844_c3_g1_i15 from sample A4 / Example spliced isoform

TGCTTCGATCGTGAGATGAATGCTTGGCTATGGTGATTGAAACTACTTTTCCATTGCACTGAAGGGTTTTGGTAGCTATTTTGCATCTTTGTTCCACTATATCGATTTGTAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGTGGGGAGGCTCACCCTGCTAGATTCTGTCAGATCTTGGTTCAAACAGTGAGTGA
TTTTGTCTTTGTACCAATTATTCTCTATCATTTACTTCTTATTTGCATCTACCCTCATTGTTGTTTGCTAATTGAGCTTTGTCTCCTTTGTTTTGCAGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACTGGC
ATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACCGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGG
TACGTATTTTATTCTTTTAACTTATCCATTTGTCATCTTGATCTCAAACCCTAGATTTGATCTTTCACAAAGAAGACGATTTGGAGGAGTGCGCATTGGTGGGGAGGCTCACCCTGCTAGCTTTTGTCAGATCTTGGTTCAAACAGGGAG

>TRINITY_DN111844_c3_g1_i15 from sample A4 / Example unspliced isoform:

TGCTTCGATCGTGAGATGAATGCTTGGCTATGGTGATTGAAACTACTTTTCCATTGCACTGAAGGGTTTTGGTAGCTATTTTGCATCTTTGTTCCACTATATCGATTTGTAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGgtggggaggctcaccctgctagattctgtcagatcttggttcaaacagtgagtga
ttttgtctttgtaccaattattctctatcatttacttcttatttgcatctaccctcattgttgtttgctaattgagctttgtctcctttgttttgcagGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACTGGC
ATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACCGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGG
TACGTATTTTATTCTTTTAACTTATCCATTTGTCATCTTGATCTCAAACCCTAGATTTGATCTTTCACAAAGAAGACGATTTGGAGGAGTGCGCATTGGTGGGGAGGCTCACCCTGCTAGCTTTTGTCAGATCTTGGTTCAAACAGGGAG

Intron 2 GTGGGGAGGC ... GATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	97.32	8	109	291
A2	97.35	6	65	220
A3	95.52	10	71	213
A4	90.59	16	35	154
A5	92.26	12	47	143
A6	100	0	1	15
A7	100	0	1	8
A8	100	0	0	11
A9	100	0	5	17
A10	100	0	2	9

>TRINITY_DN112190_c8_g3_i7 from sample A5 / Example spliced isoform

TTGGTAGCTATTTTGCATTTTTGTTCCACTATATGGATTTGCAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGTGGGGAGGCTCACCCTGCTAGCTTTTGTCAGATCTTGGTTCAAACAGGGAGTGATTTTGTCTTTGTACCAATTATTCTCTATCATTTACTTCTTATTTGCATCTACCCTCATTGTTGTTTGC
TAATTGAGCTTTGTCTCCTTGGTTTTGCAGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACCGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGAA
GAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTAAGCAAGAGAAACCACCATATCTTTCATTTGATCTTTGCGGGAGTAATCCTTGTCATATCGGAATTCATTCTCA

>TRINITY_DN112190_c8_g3_i1 from sample A5 / Example unspliced isoform:

GCTAATTGAGCTTTGTCTCCTTGATTTTGCAGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACTGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGg
tggggaggctcaccctgctagcttttgtcagatcttggttcaaacagggagtgattttgtttttgtaccaattattctctatcatttacttctcatttgcatctacactcattgttgtttgctaattgagctttgtctccttgattttgcagGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAG
AAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACCGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGG
AGTAATCCTCGTCATACCGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGTGGGGAGGCT

Intron 3 GTGGGAAGGC ... GGTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	90.91	17	24	170
A2	86.67	20	17	130
A3	82.71	23	21	110
A4	78.45	25	17	91
A5	80	19	28	76
A6	100	0	1	14
A7	100	0	1	6
A8	100	0	0	4
A9	-1	0	0	0
A10	100	0	2	9

>TRINITY_DN112190_c8_g3_i1 from sample A5 / Example spliced isoform

GCTAATTGAGCTTTGTCTCCTTGATTTTGCAGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACTGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGG
TGGGGAGGCTCACCCTGCTAGCTTTTGTCAGATCTTGGTTCAAACAGGGAGTGATTTTGTTTTTGTACCAATTATTCTCTATCATTTACTTCTCATTTGCATCTACACTCATTGTTGTTTGCTAATTGAGCTTTGTCTCCTTGATTTTGCAGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAG
AAACTACCATATCTTTCATTTGATCTTTGTGGGAGTAATCCTCGTCATACCGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGAAGAGAGGGGCGCACTAGTGGACTTGGGAAAATCGTGTGAGCATGAGAAACTACCATATCTTTCATTTGATCTTTGTGGG
AGTAATCCTCGTCATACCGGCATTCATTCTCATTATCCAGGTACGCTTGAAAGGTCAAAGAAGACGATTTGGAGGAGTGCGCATTGGTGGGGAGGCT

>TRINITY_DN112190_c8_g3_i4 from sample A5 / Example unspliced isoform:

CACTATATCGATTTGCAAGGCAAGAAGATGATTTGGGAGGAGTGCGCATTGgtgggaaggctcaccctgcttgcttctatcggattttggttcaaacaggaagtgattttgtctttgtaccaaccattctctgtcatttacttcttatttgcatctaccctcattgttgtttgctaattcagctttgtctccttggtttt
gcagGAAGAGAGGGGCGCACTAGTGGACTTGGGCAAATCGTGTGAGCAAGAGAAACTGCGAAACTTGGGAAAATCGTGTATCTTTCATTTGATCTTTGCGGGAGTAATCCTCGTCATACCAACATTCGTTCTCATTATCCAGGTACTCTTGAAAGGTACATATTTTATTCTTTTAACTTATCC