NA ----> NA

Intron 1 GTTGGCATTT ... CATTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.8	70	118	93.3
A2	59.62	51	79	70.8
A3	64.32	32.5	66	48.3
A4	61.27	49	91	60.3
A5	61.26	27.8	48	38.3
A6	65.57	67.8	137	113.8
A7	63.6	103.8	201	154.8
A8	58.04	150.3	229.5	176
A9	65.96	75.5	157.8	126.3
A10	62.73	64.3	121	90.3

>TRINITY_DN106217_c0_g4_i1 from sample A4 / Example spliced isoform

ACGCAAATATAATCTCTCTTTTCAATATCTTCAGTATGTGAAAAAGCGTATGCTGTGGAGAAGTAGTATCAATTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAATATAGGTTATATGATCTGCCATATATCATTTAATGGTCCATAAGGGTTTCATGGATCTGTCAGTACTTGGGAGCCT
TTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAAGTAGAGAT
GTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTT
CTTCAGGCGAGCGTGAGAAAAGACTTCTTTTAACCAAGAACTTATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAA

>TRINITY_DN106217_c0_g4_i2 from sample A4 / Example unspliced isoform:

ACGCAAATATAATCTCTCTTTTCAATATCTTCAGTATGTGAAAAAGCGTATGCTGTGGAGAAGTAGTATCAATTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAATATAGGTTATATGATCTGCCATATATCATTTAATGGTCCATAAGGGTTTCATGGATCTGTCAGTACTTGGGAGCCT
TTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAAGTAGAGGT
ATGCGTATTATACTTTTTGGTCACATTTGTAACTATCATAGCATTTGTTAGATTTCTAATTTCCCTATATAGATCCTTTGCTTCTAGCTCACCTTAATTGTCTAGGTAGGCTTGTTTATTTTGGGTTGTATTTGTGAAAGTTCAAATCTTCAACAGATGTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATT
CAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCGTGAGAAAAGACTTCTTTTAACCAAGAACT
TATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGgttggcatttattgttacctttggcttcatcttgtggcatggacttcaaaactttatagtggcttttctgttcattcttcagGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGAT
GAAAAA

Intron 2 GTGAGAAAAG ... CATTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.65	62	95	142
A2	69.1	36	58	103
A3	60	35	29	76
A4	57.26	53	48	94
A5	71.9	17	33	54
A6	59.3	81	75	161
A7	61.83	104	117	220
A8	60.19	127	118	266
A9	63.51	81	95	187
A10	64.02	59	74	136

>TRINITY_DN41959_c0_g2_i3 from sample A6 / Example spliced isoform

GTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGA
TATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAAACTTCAGAGTGGTTTTTCTGGTCTTCCTTCAGGTGAGTGCGAAGATGATACCCTATCAGATTGCAGCCATTTTGGTCAGT

>TRINITY_DN41959_c0_g2_i1 from sample A6 / Example unspliced isoform:

GTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCgtgagaaaagacttcttttaaccaagaacttattatatatatatatcaaagtcttcctgaatctggtgtaatgaattgattgaggttggcatttattgttacctttggcttcatcttgtggcatggacttcaaaactt
tatagtggcttttctgttcattcttcagGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATG
AATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAAACTTCAGAGTGGTTTTTCTGGTCTTCCTTCAGGTGAGTGCGAAGATGATACCCTATCAGATTGCAGCCATTTTGGTCAGT

Intron 3 GTATGCGTAT ... TCTTCAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.37	277.3	346	193
A2	45.48	218.7	238	126
A3	49.35	181	244	108
A4	50.44	175	208	148
A5	55.77	88	142	79
A6	45.89	198.3	205	131
A7	34.39	385	273	130
A8	32.31	385	226	141
A9	35.06	321.7	219	128
A10	34.46	300.3	219	96

>TRINITY_DN106217_c0_g4_i4 from sample A4 / Example spliced isoform

ACGCAAATATAATCTCTCTTTTCAATATCTTCAGTATGTGAAAAAGCGTATGCTGTGGAGAAGTAGTATCAATTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAATATAGGTTATATGATCTGCCATATATCATTTAATGGTCCATAAGGGTTTCATGGATCTGTCAGTACTTGGGAGCCT
TTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAAGTAGAGAT
GTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTT
CTTCAGGCGAGCGTGAGAAAAGACTTCTTTTAACCAAGAACTTATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGGTTGGCATTTATTGTTACCTTTGGCTTCATCTTGTGGCATGGACTTCAAAACTTTATAGTGGCTTTTCTGTTCATTCTTCAGGCGAGCGTGAGAAGATGATGCT
AGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAA

>TRINITY_DN106217_c0_g4_i2 from sample A4 / Example unspliced isoform:

ACGCAAATATAATCTCTCTTTTCAATATCTTCAGTATGTGAAAAAGCGTATGCTGTGGAGAAGTAGTATCAATTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAATATAGGTTATATGATCTGCCATATATCATTTAATGGTCCATAAGGGTTTCATGGATCTGTCAGTACTTGGGAGCCT
TTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAAGTAGAGgt
atgcgtattatactttttggtcacatttgtaactatcatagcatttgttagatttctaatttccctatatagatcctttgcttctagctcaccttaattgtctaggtaggcttgtttattttgggttgtatttgtgaaagttcaaatcttcaacagATGTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATT
CAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCGTGAGAAAAGACTTCTTTTAACCAAGAACT
TATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGGTTGGCATTTATTGTTACCTTTGGCTTCATCTTGTGGCATGGACTTCAAAACTTTATAGTGGCTTTTCTGTTCATTCTTCAGGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGAT
GAAAAA

Intron 4 GTGAGAAAAG ... ATTGATTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.34	62	75	106
A2	60	36	42	66
A3	53.02	35	30	49
A4	51.82	53	41	73
A5	66	17	27	39
A6	50.15	81	60	103
A7	52.83	104	85	148
A8	55.52	127	94	223
A9	53.04	81	67	116
A10	56.13	59	56	95

>TRINITY_DN41959_c0_g2_i3 from sample A6 / Example spliced isoform

GTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGA
TATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAAACTTCAGAGTGGTTTTTCTGGTCTTCCTTCAGGTGAGTGCGAAGATGATACCCTATCAGATTGCAGCCATTTTGGTCAGT

>TRINITY_DN41959_c0_g2_i2 from sample A6 / Example unspliced isoform:

GTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCgtgagaaaagacttcttttaaccaagaacttattatatatatatatcaaagtcttcctgaatctggtgtaatgaattgattgagGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCA
TTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTT
CGAAACTTCAGAGTGGTTTTTCTGGTCTTCCTTCAGGTGAGTGCGAAGATGATACCCTATCAGATTGCAGCCATTTTGGTCAGT