NA ----> NA

Intron 1 GTCAATATTA ... TTCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.58	75	5	16
A2	18.28	76	2	17
A3	17.2	77	2	16
A4	27.85	57	7	22
A5	25	36	0	12
A6	17.09	228	8	47
A7	21.49	296	5	81
A8	21.75	223	4	62
A9	21.81	233	4	65
A10	17	205	5	42

>TRINITY_DN99154_c0_g1_i1 from sample A4 / Example spliced isoform

TGTAAAATCACTTCTCTCCGATTCTGATTTATTGTGTGTTTCCAATTCTGATGTAAATCACTTCTCTCCGACTCTGATCTATTGAGTGTTTCAAGGAAAAGTTGGGGGGTTTAAACTTCCGTTTCGGAGAATTACAGATCCCAAGAATGGGTTTGAGGCCATTCAATTTTAAACTTACCGTTAGGCATTAGCACAACCCA
CTTACAGAAGGAGAAGGAAAACCCATTTTTCGTAACTTGCGTGCCACTCTATTCCATTGCCTCTCATCTCCGACTTCTCTGCTGCTCTGCTGGCGCCGCGTTGCCTTTTGAGATCAGGATCGGTGCTGCGAGCGGCAGCGGGCAGTATTGTGAGAGTGGGAGACGGAATAGCAGGACAGGTGCAACAGCAGCAGCAGCAG
CAGGCGGCATTCTCGGTCTGTAAAACTTCACATAAATTTTAATGGCGAAAGCAGCGGTTCCGTACGCGTGCGCCTGCCAACGGGAAAGGGGAAGAAGGGTGGTTTTGCCGGTATCAACCGCCCCCCAGACAACGTTAAACTGCAGCTGGGAGTTGTTGAATGAGTGGGAATTCTCAGGGGAAGAAAACAACGATGATAAC
TTCAGGCACCACCAATTTCGGGACCGTGTGGTGTTTGGGGCGGTGCCGAGCAAAGAGGAAGTGGAGGAGGCTGTTTCTGATTTACAGGACACCCTTACACATTCCCAGGGTTATGAAACTTCATCATTGTCAGCTTCAGTGCAGAAGAATGGTGGCTCAATTGAAGTTTCAGAGTGCTCAGACAGTGAGAGTAAATCCTC
TAAACCTGTGGTTGATTGGATAGAACCAGAATTGTATCCTTACAATCAGGAAATGGTGCAACAGTTGGGACAGAACGATATTCTCGATGCTTTCCATTTGCTACAAAGGAATCATACTGTCCGGGAGATGGTCATTTCACTGGCATCAGATAAGGCCGTCTGGGATGCTTTGTTGAAAAATGAGAAGGTTTACAAATTCA
GAAAGTTACTTCAGGAAGGTGAGACAAAGCTGCAGGAGTTAAATGACGGGTCAGACGAGAATCCTGCTGATAAGCCAAGTTGGTTTAGTCAAGCTGTTCAAAGTGCCAAGCTGAAGTTGGTTGAAATTATCCACCATATTCTCGGATTTGCAGATAAGAAGATCTTTTCAGAAAATAATGGAGAAATTCTGGACAGGACT
GTAAAGTCCTCTTTGATGCTTGCTGTTGTTGTTCTCTTGGTTGTGTTAGTCAAACGGGTTTAACATTGTAGATACTATTATGGCTGATAATTTGCTGACATGAAGATATCTCTATAGTCTTCCAGTGTTCTCTACATTTTCTATGAACATTGAGAGTACTCCAGAAATCGTTATAGAAGATATCATGTGGGTGCATCAGT
GGAGATTGTGTTCGAGTGGGCTACTAAAATTGTCTTTCCAGTAAGTTCTAATGACGATCCTTTCTATCTGAAAATGAGATCATGGCTTATTAGATTCATCCCGAATGAATAGATTCATGGCTTATTATCTTCCACATAGTTCGAGTTTTCGAGTTTTTCAGACAATGTCCTTTGAGTTTGATTCGATATTGCTTTGATAT
GTCGGTGGACAAACATGACTTGTTTTTATCTCCTTGCATTACAACATTCTATAGTTCTACAGCCCCCCGTATTTTTTGTGCA

>TRINITY_DN99154_c0_g1_i2 from sample A4 / Example unspliced isoform:

CTTGTTCATACAGCCAGGGTGTTTTTCCCTCCGTCCTTTCTATTTATAAAAGAAAACCAAAACACTCTATTAAACAATTTTGTATATCCGCCAATATGTTGCTTTAAGCACTGAACTTTTGAGTTGAGTTTTTATCAAATTTTCACCGTATTAAAGTTCCTTACAAAATAATGGACCAGAGGCTTTTAGATAGTCATTTG
GCCTTGCATGCCTCCCCTTGTTTCAATTTTAGATGTCTCATGTGTTCTTATAGTGCTCTTTTTATACAGGAGATGGTCATTTCACTGGCATCAGATAAGGCCGTCTGGGATGCTTTGTTGAAAAATGAGAAGGTTTACAAATTCAGAAAGTTACTTCAGGAAGgtcaatattattttgtttaacttactgtccagctgtt
tgactgcattccttgtttctgcatcgtttcaagcaactcccttgacctgtagacgttgatacgataaaagcttctttgcagGTGAGACAAAGCTGCAGGAGTTAAATGACGGGTCAGACGAGAATCCTGCTGATAAGCCAAGTTGGTTTAGTCAAGCTGTTCAAAGTGCCAAGCTGAAGTTGGTTGAAATTATCCACCAT
ATTCTCGGATTTGCAGATAAGAAGATCTTTTCAGAAAATAATGGAGAAATTCTGGACAGGACTGTAAAGTCCTCTTTGATGCTTGCTGTTGTTGTTCTCTTGGTTGTGTTAGTCAAACGGGTTTAACATTGTAGATACTATTATGGCTGATAATTTGCTGACATGAAGATATCTCTATAGTCTTCCAGTGTTCTCTACAT
TTTCTATGAACATTGAGAGTACTCCAGAAATCGTTATAGAAGATATCATGTGGGTGCATCAGTGGAGATTGTGTTCGAGTGGGCTACTAAAATTGTCTTTCCAGTAAGTTCTAATGACGATCCTTTCTATCTGAAAATGAGATCATGGCTTATTAGATTCATCCCGAATGAATAGATTCATGGCTTATTATCTTCCACAT
AGTTCGAGTTTTCGAGTTTTTCAGACAATGTCCTTTGAGTTTGATTCGATATTGCTTTGATATGTCGGTGGACAAACATGACTTGTTTTTATCTCCTTGCATTACAACATTCTATAGTTCTACAGCCCCCCGTATTTTTTGTGCA

Intron 2 GTTTACAAAT ... CTTCAGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.64	11	81	75
A2	83.72	14	68	76
A3	82.02	16	69	77
A4	78.42	15	52	57
A5	78.82	9	31	36
A6	84.73	40	216	228
A7	78.89	76	272	296
A8	78.72	58	206	223
A9	80.9	55	233	233
A10	85	36	203	205

>TRINITY_DN114624_c0_g1_i2 from sample A1 / Example spliced isoform

GCTTTGTTGAAAAATGAGAAGGTGAGACAAAGCTGCAGGAGTTAAATGACGGGTCAGACGAGAATCCTGCTGATAAGCCAAGTTGGTTTAGTCAAGCTGTTCAAAGTGCCAAGCTGAAGTTGGTTGAAATTATCCACCATATTCTCGGATTTGCAGATAAGAAGATCTTTTCAGAAAATAATGGAGAAATTCTGGACAGG
ACTGTAAAGTCCTCTTTGATGCTTGCTGTTGTTGTTCTCTTGGTTGTGTTAGTCAAACGGGTTTAACATTGTAGATACTATTATGGCTGATAATTTGCTGACATGAAGATATCTCTATAGTCTTCCAGTGTTCTCTACATTTTCTATGAACATTGAGAGTACTCCAGAAATCGTTATAGAAGATATCATGTGGGTGCATC
AGTGGAGATTGTGTTCGAGTGGGCTACTAAAATTGTCTTTCCAGTAAGTTCTAATGACGATCCTTTCTATCTGAAAATGAGATCATGGCTTATTAGATTCATCCCGAATGAATAGATTCATGGCTTATTATCTTCCACATAGTTCGAGTTTTCGAGTTTTTCAGACAATGTCCC

>TRINITY_DN114624_c0_g1_i1 from sample A1 / Example unspliced isoform:

TATCGTCATTAGCTTCCTTCAACCTGTTCAAACACACAATGTAAAATCACTTCTCTCCGATTCTGATTTATTGTGTGTTTCCAATTCTGATGTAAATCACTTCTCTCCGACTCTGATCTATTGAGTGTTTCAAGGAAAAGTTGGGGGGTTTAAACTTCCGTTTCGGAGAATTACAGATCCCAAGAATGGGTTTGAGGCCA
TTCAATTTTAAACTTACCGTTAGGCATTAGCACAACCCACTTACAGAAGGAGAAGGAAAACCCATTTTTCGTAACTTGCGTGCCACTCTATTCCATTGCCTCTCATCTCCGACTTCTCTGCTGCTCTGCTGGCGCCGCGTTGCCTTTTGAGATCAGGATCGGTGCTGCGAGCGGCAGCGGGCAGTATTGTGAGAGTGGGA
GACGGAATAGCAGGACAGGTGCAACAGCAGCAGCAGCAGCAGGCGGCATTCTCGGTCTGTAAAACTTCACATAAATTTTAATGGCGAAAGCAGCGGTTCCGTACGCGTGCGCCTGCCAACGGGAAAGGGGAAGAAGGGTGGTTTTGCCGGTATCAACCGCCCCCCAGACAACGTTAAACTGCAGCTGGGAGTTGTTGAAT
GAGTGGGAATTCTCAGGGGAAGAAAACAACGATGATAACTTCAGGCACCACCAATTTCGGGACCGTGTGGTGTTTGGGGCGGTGCCGAGCAAAGAGGAAGTGGAGGAGGCTGTTTCTGATTTACAGGACACCCTTACACATTCCCAGGGTTATGAAACTTCATCATTGTCAGCTTCAGTGCAGAAGAATGGTGGCTCAAT
TGAAGTTTCAGAGTGCTCAGACAGTGAGAGTAAATCCTCTAAACCTGTGGTTGATTGGATAGAACCAGAATTGTATCCTTACAATCAGGAAATGGTGCAACAGTTGGGACAGAACGATATTCTCGATGCTTTCCATTTGCTACAAAGGAATCATACTGTCCGGGAGATGGTCATTTCACTGGCATCAGATAAGGCCGTCT
GGGATGCTTTGTTGAAAAATGAGAAGgtttacaaattcagaaagttacttcaggaagGTGAGACAAAGCTGCAGGAGTTAAATGACGGGTCAGACGAGAATCCTGCTGATAAGCCAAGTTGGTTTAGTCAAGCTGTTCAAAGTGCCAAGCTGAAGTTGGTTGAAATTATCCACCATATTCTCGGATTTGCAGATAAGAAG
ATCTTTTCAGAAAATAATGGAGAAATTCTGGACAGGACTGTAAAGTCCTCTTTGATGCTTGCTGTTGTTGTTCTCTTGGTTGTGTTAGTCAAACGGGTTTAACATTGTAGATACTATTATGGCTGATAATTTGCTGACATGAAGATATCTCTATAGTCTTCCAGTGTTCTCTACATTTTCTATGAACATTGAGAGTACTC
CAGAAATCGTTATAGAAGATATCATGTGGGTGCATCAGTGGAGATTGTGTTCGAGTGGGCTACTAAAATTGTCTTTCCAGTAAGTTCTAATGACGATCCTTTCTATCTGAAAATGAGATCATGGCTTATTAGATTCATCCCGAATGAATAGATTCATGGCTTATTATCTTCCACATAGTTCGAGTTTTCGAGTTTTTCAG
ACAATGTCCC