sp|O22928|PUSH_ARATH ----> Putative tRNA pseudouridine synthase

Intron 1 GTGAGTAACA ... TTCTAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	3	6
A2	70.37	1.8	2	6
A3	100	0	1	0
A4	67.68	1.8	6	1
A5	82.22	1.8	9	7
A6	71.84	15.9	40	40
A7	72.94	12.3	24	42
A8	72.28	15.8	37	44
A9	60.13	15.9	18	29
A10	83.4	8.1	44	36

>TRINITY_DN41258_c0_g1_i6 from sample A6 / Example spliced isoform

ACCAGATGGAGGAGGATGGTCATTGTCATCAAATGTCCCTACTGAGAGCATGGTCTTTACCTTCAATAGTAGTCATATAGGGATGAGTATTGTAGTGTCGATGATGGTTTTTCTATTGATGCCTTCTGGATTCATTGAAGTTCCGAGATTGGAAACTTCTTGACTCAGTTTATGTCTTTGCAATTGGCATGGCACCATAA
GAATTGATACATGTAGGCTCTTTGTGCTATATGGCTAGAAGTTGGGTTTTGTATAAATTGGTAGTCCCAAACTCCCAAGCACACAAGGGACTGATTTCTTGTGTAGACAAGAAGCTAAACATTATAAGTTGAATGAGAGGCATTGCAATGCATTCGATGTTAAAATTTATAATGTGAAACAATTTTTTGAAGTTATTAAA
CGCAATTTCTGGAGAACATAATTCTTTGATGGATCATATGACTAGTGAAAGGAGATTTTGTCTTCCTTGAACCAATTTTTTTTCATGTGTATTGGTTGTGAAATGGAACAAAAGGCATTTACTAACCAGGGTA

>TRINITY_DN41258_c0_g1_i4 from sample A6 / Example unspliced isoform:

CTATTATATAACTTGAACCCACCTTTTTGAGGATACATTACATGAACTGCAGCGTTAATTTTCAAAATGAGAAAGTTTATTATGAAATTCTAAACGAAGGATGAAGCTGATCAGAGAAACTTCAAACACTGATGTCTGATAGATTCATAGAGCTAAATGCTATTGATAATAGGAAGAGAGCTCTTGACAACGATACTCAA
AGCATGAATTCCAAAAAAGTGTTGAGAGACGAAAAACCAGAGCAAGACGAAGGGGACAACGAAGACATGAGTCAACGGCCAGGAGTTCAGAGATATTTGGTCGCAGTGGAATACATTGGCACAAATTTCCTTGGCATGCAAAAACAGCCCAATTTCAGAACAGTTCAGGGCGTGCTCGAGGATGCATTTAACAAATTTGT
TGGGCAGCCTGTTTCTAGTGCAACCTCAAGTAGAACTGATGCAGGAGTTCATGCATTGGCAAATGTATGCCATGTAGATGTCGAGCGCATCAGTAAGCGGAAGCCTGGTGAAGTGTTACCTCCACATGAGCCTGAAGTTGTGAAAAGAGCCGTGAACCACTTTTTACAGAAAGATGCAGGTGATATTTCGATCATTGATG
TCCGATTTGTGCCGATGACTTTTCATGCTCGCTTTAGAGCTCAGGAGCGCACATATTTCTACAGGATTCTTTCTGGTAAAGAGCCTTTATCTGCTTTTGAAAAAGATCGAGCGTGGCACATCCCTGAAGACTTAGGTCTCTCACGTATGCAGGAAGCATGTAAAGTCCTTGTAGGACACCATGATTTTAGCTCCTTCCGA
GCCGCAGGATGTCAGGCAAAATCCCCTATCAAAACCTTGGATGAACTCTATGTTTGTGAAATGCCAGCGTGGCCTGCCTTTCCATCAAGCATAGAGAGGAATAGTCTAGTAAACATTGCTGCAGCAAGTTGTAAACATGTGAACTCTGAAGGAAAGGCGAGTTTTGATAATCCTGTTGAAATGGAAGGGCGGGTATCTCT
TTCTGAGGAAAGACATATGAATCATGATCAGCCTGATATGCTTTGTCAAGAAGAGAATGGAAATTTTAGTCAGGGGGATGTTTTTGAATTTGGTCGCAGAAGAGGGCATCGTGGCTATGTAGTTGTAGCAAGGGCACAGTCATTTTTGTATCACCAGGTTCGGTTACTTGTTGGTACCTTGAAGTGTGTTGGAACTGGAA
AACTGTCTGTTGATGATGTTGAAAGAATTCTTAAAGCACGGACAATAACAGTAGCACCACCTATGGCCCCTGCCTGTGGCCTGTATCTTGCAGCAGTTAAGTATGACTTCAGTTGTAATGATGTGAACCAGATGGAGGAGGATGgtgagtaacagagacaaatctttgttcttaagttcagtgccacttccagattcgat
ttatgcaaaatattccatgatttagatgccttcatggaccaattcattttctaagcagGTCATTGTCATCAAATGTCCCTACTGAGAGCATGGTCTTTACCTTCAATAGTAGTCATATAGGGATGAGTATTGTAGTGTCGATGATGGTTTTTCTATTGATGCCTTCTGGATTCATTGAAGTTCCGAGATTGGAAACTTCT
TGACTCAGTTTATGTCTTTGCAATTGGCATGGCACCATAAGAATTGATACATGTAGGCTCTTTGTGCTATATGGCTAGAAGTTGGGTTTTGTATAAATTGGTAGTCCCAAACTCCCAAGCACACAAGGGACTGATTTCTTGTGTAGACAAGAAGCTAAACATTATAAGTTGAATGAGAGGCATTGCAATGCATTCGATGT
TAAAATTTATAATGTGAAACAATTTTTTGAAGTTATTAAACGCAATTTCTGGAGAACATAATTCTTTGATGGATCATATGACTAGTGAAAGGAGATTTTGTCTTCCTTGAACCAATTTTTTTTCATGTGTATTGGTTGTGAAATGGAACAAAAGGCATTTACTAACCAGGGTA