sp|Q6ZIX2|SMT1_ORYSJ ----> Cycloartenol-C-24-methyltransferase 1

Intron 1 GTACTCCTTG ... AAGGGGGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.73	530	554	836
A2	58.69	434	462	771
A3	58.71	411	396	773
A4	59.98	328	414	569
A5	60.55	172	210	318
A6	59.84	1523	1275	3263
A7	58.78	2121	1784	4264
A8	59.43	2300	1689	5049
A9	59.69	2109	1840	4407
A10	57.16	1828	1455	3423

>TRINITY_DN132535_c0_g1_i5 from sample A1 / Example spliced isoform

GATTTTTGCTGATCAATCCAGGTTTCCAGCTTTAGAAAACAACATGCTCATGTTATTCCCGTGACGTGTCACCCTTTCACAGTGTCACCCTTTCACCGGAAGGTCGCGTGATCAATTCCCTGGCGCTGCCATTGAAATAGACGAATCTCCATTATCTCTATATAGCTGGCTACACTAAGAGTTTGTTTTGTCCCTTAATC
TATTGCCACCAGCTATTGCTTCCTTGTCAGAAGTCGAAGTAGTTTACGTTCTGCGCGGGGATTCCTGCCGAATCATCACAGTCAAGCTATTAACGATTTTGCGGTTCAAGAAAGCTTCACTGAATCGCTCGATCAAGCTGTGTTTCAGGAATGGCAACGGTAGGAGGAGCGATAGATCTGGCTTCGGGAGTGGGAGGCAA
CATTCAGAAAAGCCAAATGCGGACTGCTGTCGAAGAGTATGAAAAATATCACATGTACTATGGTGGTGATGAAGGCGCGAGAAAATCTAACTACACGGATATGGTAAATAAATACTATGATCTGGCTACTAGTTTCTATGAGTATGGATGGGGAGAATCTTTTCATTTTGCTCACAGATGGAAAGGGGAGTCCCTTCGAG
AAAGTATAAAGCGCCATGAACATTTTCTTGCTCTCCAGCTTTGTTTAAAGCCTGCAATGAAGGTATTGGATGTAGGATGTGGGATTGGAGGTCCACTGAGAGAAATTGCTAGGTTCAGTCGGAGTTCGATCACAGGATTAAATAATAATGCTTACCAAATGTCAAGAGGAAAAGAACTTAACAGGAAATGTGGGCTAGAC
AAAAGCTGTGATTTTGTTAAGGGAGACTTCATGAAGATGCCTTTCTCCGATGACACGTTTGATGCAGTTTATGCAATTGAGGCTACTTGTCATGCACCAGATGCAGTTGGTTGCTACGAAGAGATAAAAAGAGTACTAAAACCTGGTCAACTGTTTGCGGCATATGAATGGTGCATGACTGACTCTTTCGACCCAAAGAA
TGAAGAACACCAGAAGATTAAGGCAGAAATTGAACTTGGAAATGGGCTTCCTGATGTTCGTACTACAAGCCAGTGTCTTGAAGCCCTTAAATCTGCTGGATTTGAGGAAGGAGATTTTTACACCTATGTACTTTTTTGTGGCCCGAAAACCTTTGAATGATACCCAGTCATGAAGTTCAACATATTTTTCACTGGAACAG
AGCCTATGCCACTTTTGTTTACGAACAGCTAAACAGTTAGTTTCTTGATGTTAAGGAATTGTTTTTTTCTTGTTTCATTGCTTGGCCTTTCAACAGTGAAAGGAAATAAAGACAAACGATTGTAAAAATCATATTCCAGTCAGCTCAGTTGTAGAAATCAATATTAGGCAAGAGAAAGAAGCTTGGGTTGTTCTTGGTTT
GTTAGTTGAAGACAGTGACAGGACAGGAATTGCCCATGGATTCATTTGTACATGTAATATCTTTTGTTACTGGCTGGGTTTTCATCAATCTAAATTGTTGCTTTCCAGTTCCTAAAATCACTATGTTTAACCAAAGTAGTAAGCATTTGATAGAAACATTGTGAGCATTCCATAAATTTCCAAAATCATAGATGTTCACA
AGTTTGTTCTAAAAGTATATAGTCC

>TRINITY_DN132535_c0_g1_i2 from sample A1 / Example unspliced isoform:

TGATAGTGTGTGCGTTCATTGTGAGATTCTTTGCTCTATCCAAAAATGCCGTTGAAAATGCTGTCCAGGAGCTTGTGTAAAGCTTTGAAGATTCTGCATTGCTTCTTTTATTTTATTTTATTTTTGGTTAGATCTGCTTGCTAACCTGTGAAGTTTGTTCGGAAAATAATCTTCAGAACCTCTACATAAAAGATTTGATG
CTAACATTTACAATGGGAAAATGCTTTAAAGATTTGATTTAAGTTTGTTTGGAAAAATCTCTTTATGATATATGCACAGGCTTTGTTAATATTTACGAGATGCATGATAGTGATTTTAGATGCTTGATTATATTTCAGGAATGGCAACGGTAGGAGGAGCGATAGATCTGGCTTCGGGAGTGGGAGGCAACATTCAGAAA
AGCCAAATGCGGACTGCTGTCGAAGAGTATGAAAAATATCACATGTACTATGGTGGTGATGAAGGCGCGAGAAAATCTAACTACACGGATATGGTAAATAAATACTATGATCTGGCTACTAGTTTCTATGAGTATGGATGGGGAGAATCTTTTCATTTTGCTCACAGATGGAAAGGGGAGTCCCTTCGAGAAAGTATAAA
GCGCCATGAACATTTTCTTGCTCTCCAGCTTTGTTTAAAGCCTGCAATGAAGGTATTGGATGTAGGATGTGGGATTGGAGGTCCACTGAGAGAAATTGCTAGGTTCAGTCGGAGTTCGATCACAGGATTAAATAATAATGCTTACCAAATGTCAAGAGGAAAAGAACTTAACAGGAAATGTGGGCTAGACAAAAGCTGTG
ATTTTGTTAAGGGAGACTTCATGAAGATGCCTTTCTCCGATGACACGTTTGATGCAGTTTATGCAATTGAGGCTACTTGTCATGCACCAGATGCAGTTGGTTGCTACGAAGAGATAAAAAGAGTACTAAAACCTGGTCAACTGTTTGCGGCATATGAATGGTGCATGACTGACTCTTTCGACCCAAAGAATGAAGAACAC
CAGAAGATTAAGGCAGAAATTGAACTTGGAAATGGGCTTCCTGATGTTCGTACTACAAGCCAGTGTCTTGAAGCCCTTAAATCTGCTGGATTTGAGgtactccttgaagagaaccttacagtctcatcagcagtggcgtggtattcaccactggataaaagtcaattctctctgagtagttttcgtcttacagcgcttgg
tcgctttttgacaagaaatatggttagaatattggaattcctgagggtagcaccggaaggcagtgaaagggtatcttcatttttggagaaagctgcagaaggccttgttgaaggggggagGAAGGAGATTTTTACACCTATGTACTTTTTTGTGGCCCGAAAACCTTTGAATGATACCCAGTCATGAAGTTCAACATATT
TTTCACTGGAACAGAGCCTATGCCACTTTTGTTTACGAACAGCTAAACAGTTAGTTTCTTGATGTTAAGGAATTGTTTTTTTCTTGTTTCATTGCTTGGCCTTTCAACAGTGAAAGGAAATAAAGACAAACGATTGTAAAAATCATATTCCAGTCAGCTCAGTTGTAGAAATCAATATTAGGCAAGAGAAAGAAGCTTGG
GTTGTTCTTGGTTTGTTAGTTGAAGACAGTGACAGGACAGGAATTGCCCATGGATTCATTTGTACATGTAATATCTTTTGTTACTGGCTGGGTTTTCATCAATCTAAATTGTTGCTTTCCAGTTCCTAAAATCACTATGTTTAACCAAAGTAGTAAGCATTTGATAGAAACATTGTGAGCATTCCATAAATTTCCAAAAT
CATAGATGTTCACAAGTTTGTTCTAAAAGTATATAGTCC

Intron 2 GTATGTATTT ... TTTGAGATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.18	1458	3	261
A2	14.67	1355	2	233
A3	15.17	1459	0	261
A4	15.85	1104	2	208
A5	13.91	557	1	90
A6	13.54	3064	1	480
A7	13.61	5146	0	811
A8	14.51	3871	0	657
A9	15.13	5055	0	901
A10	14.12	4651	0	765

>TRINITY_DN132535_c0_g1_i3 from sample A1 / Example spliced isoform

GCTCGATCAAGCTGTGTTTCAGGTGCGTTCATTGAATGGCAACGGTAGGAGGAGCGATAGATCTGGCTTCGGGAGTGGGAGGCAACATTCAGAAAAGCCAAATGCGGACTGCTGTCGAAGAGTATGAAAAATATCACATGTACTATGGTGGTGATGAAGGCGCGAGAAAATCTAACTACACGGATATGGTAAATAAATAC
TATGATCTGGCTACTAGTTTCTATGAGTATGGATGGGGAGAATCTTTTCATTTTGCTCACAGATGGAAAGGGGAGTCCCTTCGAGAAAGTATAAAGCGCCATGAACATTTTCTTGCTCTCCAGCTTTGTTTAAAGCCTGCAATGAAGGTATTGGATGTAGGATGTGGGATTGGAGGTCCACTGAGAGAAATTGCTAGGTT
CAGTCGGAGTTCGATCACAGGATTAAATAATAATGCTTACCAAATGTCAAGAGGAAAAGAACTTAACAGGAAATGTGGGCTAGACAAAAGCTGTGATTTTGTTAAGGGAGACTTCATGAAGATGCCTTTCTCCGATGACACGTTTGATGCAGTTTATGCAATTGAGGCTACTTGTCATGCACCAGATGCAGTTGGTTGCT
ACGAAGAGATAAAAAGAGTACTAAAACCTGGTCAACTGTTTGCGGCATATGAATGGTGCATGACTGACTCTTTCGACCCAAAGAATGAAGAACACCAGAAGATTAAGGCAGAAATTGAACTTGGAAATGGGCTTCCTGATGTTCGTACTACAAGCCAGTGTCTTGAAGCCCTTAAATCTGCTGGATTTGAGGTACTCCTT
GAAGAGAACCTTACAGTCTCATCAGCAGTGGCGTGGTATTCACCACTGGATAAAAGTCAATTCTCTCTGAGTAGTTTTCGTCTTACAGCGCTTGGTCGCTTTTTGACAAGAAATATGGTTAGAATATTGGAATTCCTGAGGGTAGCACCGGAAGGCAGTGAAAGGGTATCTTCATTTTTGGAGAAAGCTGCAGAAGGCCT
TGTTGAAGGGGGGAGGAAGGAGATTTTTACACCTATGTACTTTTTTGTGGCCCGAAAACCTTTGAATGATACCCAGTCATGAAGTTCAACATATTTTTCACTGGAACAGAGCCTATGCCACTTTTGTTTACGAACAGCTAAACAGTTAGTTTCTTGATGTTAAGGAATTGTTTTTTTCTTGTTTCATTGCTTGGCCTTTC
AACAGTGAAAGGAAATAAAGACAAACGATTGTAAAAATCATATTCCAGTCAGCTCAGTTGTAGAAATCAATATTAGGCAAGAGAAAGAAGCTTGGGTTGTTCTTGGTTTGTTAGTTGAAGACAGTGACAGGACAGGAATTGCCCATGGATTCATTTGTACATGTAATATCTTTTGTTACTGGCTGGGTTTTCATCAATCT
AAATTGTTGCTTTCCAGTTCCTAAAATCACTATGTTTAACCAAAGTAGTAAGCATTTGATAGAAACATTGTGAGCATTCCATAAATTTCCAAAATCATAGATGTTCACAAGTTTGTTCTAAAAGTATATAGTCC

>TRINITY_DN132535_c0_g1_i8 from sample A1 / Example unspliced isoform:

ACTTGTCATGCACCAGATGCAgtatgtatttgcaagtttcccccttaaacaacactattgccattgtacttttgtgatgtatgtgtgcccatcatattatggttgtaacaccctgaaaatcagactgacactttcagaatcagttattatggagaagtctatgaagcaaacatattccatgtttacacaagtgtttctga
ttcttaccagcgaactgtttataactgtttgagatagGTTGGTTGCTACGAAGAGATAAAAAGAGTACTAAAACCTGGTCAACTGTTTGCGGCATATGAATGGTGCATGACTGACTCTTTCGACCCAAAGAATGAAGAACACCAGAAGATTAAGGCAGAAATTGAACTTGGAAATGGGCTTCCTGATGTTCGTACTACAA
GCCAGTGTCTTGAAGCCCTTAAATCTGCTGGATTTGAGG