sp|P16866|H2A4_VOLCA ----> Histone H2A-IV

Intron 1 GTAATAATCT ... TTTTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.48	10	2	9
A2	23.08	5	0	3
A3	6.67	7	1	0
A4	41.18	5	3	4
A5	33.33	4	2	2
A6	18.18	36	11	5
A7	24.06	101	33	31
A8	21.43	77	28	14
A9	20.93	204	75	33
A10	22.22	98	36	20

>TRINITY_DN41456_c0_g1_i3 from sample A7 / Example spliced isoform

CGTTGTAGCTCAAGAAGATTTGGAGAGTAACTTAGGTTATTTCTGGTGTATTTCGTTTTCTATCCGTTAATGCTTCCTGTCTGAGCCATAAAACTGTTCACAATAAGATAACCCTGAAGTGATCATGGCGATCAAAACGATCCATGTTCCTAGCAATCCTTCCACTTCGCCAATAAATATTGCACGGCTTGCGGCGGGAA
AATATAAAATTCCTTATCAGAAACGAGTCTCAAAATCGACAAAGGCAGGACTGCTATTCCCCGTGAGCAGGATTTCAAGATACCTCAAGATGGGAAATTATGCGGCCAGAGTTAGTGAATCTGCTGCCATCACCCTTACTTCTGTCATTCAGTATCTCATTGAGGAGTTACTGATGTTGGCCGGAGAAGCTGCTATGGGC
ACCAAGAAGAGACGAATTACTCCAAGACACGTTCTCTTGGCAATCAAAGGGGATGATGAATTCCGTAAGCTTCTGGAAAATACTACTATTCCTAGTGCTGGCGTTGTTGCTCAAATTCATGAGGAGCTTCTGCCCTCTAAGAAGAGAAAACAAACCTCCTCTCAGAAAAGAGAAAACCTTAGCTAAATCCCTGGACTTCA
ATTTAGGTTTGGTTGAACTCATAACCTATTCACGAGATTTTTATTGATATATTTCTTAGTTTGCAACCGTAAGAACATGCATTACGGAATTTGTGAATCCAATAAACATATGGGTGTTTGTAACTGGACAATTATAACTCCCAAATAATATAAAAGACATTTCAACTCTCAAATA

>TRINITY_DN41456_c0_g1_i6 from sample A7 / Example unspliced isoform:

CGTTGTAGCTCAAGAAGATTTGGAGAGTAACTTAGGTTATTTCTGGTGTATTTCGTTTTCTATCCGTTAATGCTTCCTGTCTGAGCCATAAAACTGTTCACAATAAGATAACCCTGAAGTGATCATGGCGATCAAAACGATCCATGTTCCTAGCAATCCTTCCACTTCGCCAATAAATATTGCACGGCTTGCGGCGGGAA
AATATAAAATTCCTTATCAGAAACGAGTCTCAAAATCGACAAAGGCAGGACTGCTATTCCCCGTGAGCAGGATTTCAAGATACCTCAAGATGGGAAATTATGCGGCCAGAGTTAGTGAATCTGCTGCCATCACCCTTACTTCTGTCATTCAGTATCTCATTGAGGAGgtaataatcttcaatctatgttatgcgattctt
taatatttctatggtgcctaaatattttgaattcttttcttcagTTACTGATGTTGGCCGGAGAAGCTGCTATGGGCACCAAGAAGAGACGAATTACTCCAAGACACGTTCTCTTGGCAATCAAAGGGGATGATGAATTCCGTAAGCTTCTGGAAAATACTACTATTCCTAGTGCTGGCGTTGTTGCTCAAATTCATGAG
GAGCTTCTGCCCTCTAAGAAGAGAAAACAAACCTCCTCTCAGAAAAGAGAAAACCTTAGCTAAATCCCTGGACTTCAATTTAGGTTTGGTTGAACTCATAACCTATTCACGAGATTTTTATTGATATATTTCTTAGTTTGCAACCGTAAGAACATGCATTACGGAATTTGTGAATCCAATAAACATATGGGTGTTTGTAA
CTGGACAATTATAACTCCCAAATAATATAAAAGACATTTCAACTCTCAAATA

Intron 2 GTTAGTGAAT ... TTTTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40	3	2	9
A2	50	3	3	3
A3	40	3	2	0
A4	33.33	6	3	4
A5	66.67	2	4	2
A6	56.34	31	40	5
A7	56.67	52	68	31
A8	70.83	21	51	14
A9	64.45	75	136	33
A10	57.74	71	97	20

>TRINITY_DN41456_c0_g1_i4 from sample A7 / Example spliced isoform

CGTTGTAGCTCAAGAAGATTTGGAGAGTAACTTAGGTTATTTCTGGTGTATTTCGTTTTCTATCCGTTAATGCTTCCTGTCTGAGCCATAAAACTGTTCACAATAAGATAACCCTGAAGTGATCATGGCGATCAAAACGATCCATGTTCCTAGCAATCCTTCCACTTCGCCAATAAATATTGCACGGCTTGCGGCGGGAA
AATATAAAATTCCTTATCAGAAACGAGTCTCAAAATCGACAAAGGCAGGACTGCTATTCCCCGTGAGCAGGATTTCAAGATACCTCAAGATGGGAAATTATGCGGCCAGATTACTGATGTTGGCCGGAGAAGCTGCTATGGGCACCAAGAAGAGACGAATTACTCCAAGACACGTTCTCTTGGCAATCAAAGGGGATGAT
GAATTCCGTAAGCTTCTGGAAAATACTACTATTCCTAGTGCTGGCGTTGTTGCTCAAATTCATGAGGAGCTTCTGCCCTCTAAGAAGAGAAAACAAACCTCCTCTCAGAAAAGAGAAAACCTTAGCTAAATCCCTGGACTTCAATTTAGGTTTGGTTGAACTCATAACCTATTCACGAGATTTTTATTGATATATTTCTT
AGTTTGCAACCGTAAGAACATGCATTACGGAATTTGTGAATCCAATAAACATATGGGTGTTTGTAACTGGACAATTATAACTCCCAAATAATATAAAAGACATTTCAACTCTCAAATA

>TRINITY_DN41456_c0_g1_i6 from sample A7 / Example unspliced isoform:

CGTTGTAGCTCAAGAAGATTTGGAGAGTAACTTAGGTTATTTCTGGTGTATTTCGTTTTCTATCCGTTAATGCTTCCTGTCTGAGCCATAAAACTGTTCACAATAAGATAACCCTGAAGTGATCATGGCGATCAAAACGATCCATGTTCCTAGCAATCCTTCCACTTCGCCAATAAATATTGCACGGCTTGCGGCGGGAA
AATATAAAATTCCTTATCAGAAACGAGTCTCAAAATCGACAAAGGCAGGACTGCTATTCCCCGTGAGCAGGATTTCAAGATACCTCAAGATGGGAAATTATGCGGCCAGAgttagtgaatctgctgccatcacccttacttctgtcattcagtatctcattgaggaggtaataatcttcaatctatgttatgcgattctt
taatatttctatggtgcctaaatattttgaattcttttcttcagTTACTGATGTTGGCCGGAGAAGCTGCTATGGGCACCAAGAAGAGACGAATTACTCCAAGACACGTTCTCTTGGCAATCAAAGGGGATGATGAATTCCGTAAGCTTCTGGAAAATACTACTATTCCTAGTGCTGGCGTTGTTGCTCAAATTCATGAG
GAGCTTCTGCCCTCTAAGAAGAGAAAACAAACCTCCTCTCAGAAAAGAGAAAACCTTAGCTAAATCCCTGGACTTCAATTTAGGTTTGGTTGAACTCATAACCTATTCACGAGATTTTTATTGATATATTTCTTAGTTTGCAACCGTAAGAACATGCATTACGGAATTTGTGAATCCAATAAACATATGGGTGTTTGTAA
CTGGACAATTATAACTCCCAAATAATATAAAAGACATTTCAACTCTCAAATA

Intron 3 GTTAGTGAAT ... CATTGAGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.71	3	1	10
A2	62.5	3	5	5
A3	70	3	7	7
A4	36.84	6	2	5
A5	71.43	2	6	4
A6	60.26	31	58	36
A7	66.67	52	107	101
A8	79	21	81	77
A9	73.59	75	214	204
A10	62.23	71	136	98

>TRINITY_DN35982_c0_g2_i2 from sample A6 / Example spliced isoform

ATTAACATTTGAAATTTCATTACCGTTGTAGCTCAAGAAGATTTGGAGAGTAACTTAGGTTATTTCTGGTGTATTTCGTTTTCTATCCGTTAATGCTTCCTGTCTGAGCCATAAAACTGTTCACAATAAGATAACCCTGAAGTGATCATGGCGATCAAAACGATCCATGTTCCTAGCAATCCTTCCACTTCGCCAATAAA
TATTGCACGGCTTGCGGCGGGAAAATATAAAATTCCTTATCAGAAACGAGTCTCAAAATCGACAAAGGCAGGACTGCTATTCCCCGTGAGCAGGATTTCAAGATACCTCAAGATGGGAAATTATGCGGCCAGATTACTGATGTTGGCCGGAGAAGCTGCTATGGGCACCAAGAAGAGACGAATTACTCCAAGACACGTTC
TCTTGGCAATCAAAGGGGATGATGAATTCCGTAAGCTTCTGGAAAATACTACTATTCCTAGTGCTGGCGTTGTTGCTCAAATTCATGAGGAGCTTCTGCCCTCTAAGAAGAGAAAACAAACCTCCTCTCAGAAAAGAGAAAACCTTAGCTAAATCCCTGGACTTCAATTTAGGTTTGGTTGAACTCATAACCTATTCACG
AGATTTTTATTGATATATTTCTTAGTTGGCAACCGTAAGAACATGCATTACGGAATTTGTGAATCCAATAAACATATGGGTGTTTGTAACTGGACAATTATAACTCCCAAATAATATAAAAGACATTTCAACTCTCA

>TRINITY_DN35982_c0_g2_i1 from sample A6 / Example unspliced isoform:

ATTAACATTTGAAATTTCATTACCGTTGTAGCTCAAGAAGATTTGGAGAGTAACTTAGGTTATTTCTGGTGTATTTCGTTTTCTATCCGTTAATGCTTCCTGTCTGAGCCATAAAACTGTTCACAATAAGATAACCCTGAAGTGATCATGGCGATCAAAACGATCCATGTTCCTAGCAATCCTTCCACTTCGCCAATAAA
TATTGCACGGCTTGCGGCGGGAAAATATAAAATTCCTTATCAGAAACGAGTCTCAAAATCGACAAAGGCAGGACTGCTATTCCCCGTGAGCAGGATTTCAAGATACCTCAAGATGGGAAATTATGCGGCCAGAgttagtgaatctgctgccatcacccttacttctgtcattcagtatctcattgaggagTTACTGATGT
TGGCCGGAGAAGCTGCTATGGGCACCAAGAAGAGACGAATTACTCCAAGACACGTTCTCTTGGCAATCAAAGGGGATGATGAATTCCGTAAGCTTCTGGAAAATACTACTATTCCTAGTGCTGGCGTTGTTGCTCAAATTCATGAGGAGCTTCTGCCCTCTAAGAAGAGAAAACAAACCTCCTCTCAGAAAAGAGAAAAC
CTTAGCTAAATCCCTGGACTTCAATTTAGGTTTGGTTGAACTCATAACCTATTCACGAGATTTTTATTGATATATTTCTTAGTTGGCAACCGTAAGAACATGCATTACGGAATTTGTGAATCCAATAAACATATGGGTGTTTGTAACTGGACAATTATAACTCCCAAATAATATAAAAGACATTTCAACTCTCA