sp|Q6H849|NRL4_ORYSJ ----> Bifunctional nitrilase/nitrile hydratase NIT4

Intron 1 GTAGTGACTC ... CTCTTGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.86	3	11	18
A2	76.47	2	5	8
A3	77.78	2	5	9
A4	69.23	2	3	6
A5	76	3	11	8
A6	40.43	14	5	14
A7	56.92	14	18	19
A8	86.05	3	19	18
A9	52.94	16	10	26
A10	43.21	23	14	21

>TRINITY_DN47661_c0_g1_i1 from sample A7 / Example spliced isoform

GGGTAAAGTGGAAGCGGGCAAAGTTTCTTGAAAAGCAAATCAAGCTTTTTTGTGATAGACGTTGTGGTAAAACTGCAATCCGCTCCTCTAACGGCAGGTCAAGCTTGTTTTTTTTGAGATAGACGAGGGAGATAATCTATCCGTTAAGGAAAAAGTGCGGAAGGGTAGAAGTTGGTGTATATATATGTCTAGACGAATTA
TAATTGAATCCCTTCTGTTCACATCCTCCTCTTTTATCATTTTCATTTGCAGGGAATTTTCTTTTGTTTGCAGTGGAAGCGCTTCCTCCTGTTCATGCATGTCTGTGTGGGATGCACATGTTAATGAAGAACGTTGCTGCTCGATGCCATTGAGTTAATAAGTTGAATCGACAGAATGGAGGTAATTACCGTGGAGAGTA
GAGGACCGGACCGCAACAACAATATTTGCTGCAGCGCAGCCATAATAGGTATGTGATTTGTATTTATGTCATATTCATGGTTTTGAGTACATAGATTAAATTAGAGTTTTCTTGGCTAATTAGCAGATCGTCCTTCCTCATCAAAGCCCATGTCCCTCCCAATCAATCTGTCATTCCATCCTTCAATGTGATTTTTCATC
CCCTGTTTTGATGAGATTTCCCCTTCTCTGTATTGATGGGGACACGGGACGAAAAATCGCCCCCTGCCTTAACGCCGCTTAACCTTACCGCCACTTAGATTAATGCTCTTTTTAAAAACTTGGAATGGATAGGGGAGGCTCGATTCAAGCTTCAACCACAATTTCGAATCTATCGCAATTTGTTTTTTCAGGAATTCAAG
GCGACCTCTACATAGGGACCCATTGTTCTGTACTTGGAGGATCGTTATTTACTAGCTAGATCTCGTTGGTCTATAGTTGGGTGTGATCATTGGTCCTTGGTTCTTCAAGTCATGGACCTACTCGATATTACTCCTCGCTTTCCCTTCCCCGCATTGTTTAGAGCCTTTGATCGCTGCTGAATTAGGGTTTAATAGCTAGA
TCAATCACATATAAGTAAGGCAAGATGTCCGGGTGCTTTCATTTCCCCTAATTGTTTAGAGCCATTGATCGCTGGTGAATTGGAATTTAATAGCTAGATAAATCATATACAAGGCAAGATGCTCAAACAGATAGATCATATATAAGTAACAAATAACAACGGTAAAACTATATCGAAATCTCTTCCCGAAATGCCGAAAT
GGAGAGCTTCACCCGTGTGGGAAACTAAGGTAGATCAGAAGATCCAACTGAAATAGTTGTCCAAGAGATCTTAAAAAACCAACTACCATGATCGGTAACGATATATATTATGGTATGGTATGGTATTGACGTCACGTCGATCTGAACCCAAGTTGGTTTTCTGAACCCAATAGTTGAAATCATAGTGGTATAGCTCGGAG
AAATAAATAAGGGTTTGTTCCTTGCAATAAAACACGCCAAACCAACGTGGCCAGGTGTGGCCTAGACATGCTTTAGCAGAACTGTATCTCCTTCGCCGTGATTTTTTACTTGACCGTCCAACTGTGAATGTATGTCACGGACGGAGAGAGTGGGGCGTCAGGGTTGGCTAGCGTCCTATTCTCGGGCTTTCATCCACCTA
TTTCAATGAAGGCACTTCGATCAACCTTTCGAATGATGCTACTTGCAAGCGGGCTGTCGAGTAAAGGGGCGGGTTTAGCACCTATGGATGTGAATGGATTTTCCCATTTTTATTCATCTGCTTAGGTCCAACCATCTTAGGACTGTATCTTTTCTTATCTTACTAGCAACCAATTCCATACTCCATTCAGTATAGTTTGG
ATAAGGGGCCTGAAATGAGGGAAGAGGATCAAGTGAAAGTGGTATAGAATTTATTTTTCCTATTTCCATCTGTTTATTGAGATTCTTTGCATTACGGTTGGAGCTAGAATAGAGTTTTGTTTCCCGGTTTCCATCGGTTTATTGAGATTCTTTGCATTACGGTTAGAGCTGACCCATAGGAAAGAATAGGTTCTGCCTTC
TGTTTACCAAGTTGTTGTACCTCCCCCAAAACTGCTATTATTCTTCCATTAACAACTAGAACCAACAATATGTTTCTAGGTGTCCCGGTTGTCTCAGAATCCATCACGAGATCGAATTCCAAAAGAACACTAAACTTGAAGAAACTAAACGAGAAAGCCCACTGTCTCGCTCTCTATGTCGACACGCACTACATGGAGTT
AAACATGTATTTATGATTTGGTAAGCCTGGTAGAGTTGGTTTTTGAATGTGC

>TRINITY_DN47661_c0_g1_i4 from sample A7 / Example unspliced isoform:

GGGTAAAGTGGAAGCGGGCAAAGTTTCTTGAAAAGCAAATCAAGCTTTTTTGTGATAGACGTTGTGGTAAAACTGCAATCCGCTCCTCTAACGGCAGGTCAAGCTTGTTTTTTTTGAGATAGACGAGGGAGATAATCTATCCGTTAAGGAAAAAGTGCGGAAGGGTAGAAGTTGGTGTATATATATGTCTAGACGAATTA
TAATTGAATCCCTTCTGTTCACATCCTCCTCTTTTATCATTTTCATTTGCAGGGAATTTTCTTTTGTTTGCAGTGGAAGCGCTTCCTCCTGTTCATGCATGTCTGTGTGgtagtgactctgtgtcttgttcctgtttgtcggtattttgtttctgtgtcgcgttgtgtgtctatctgtatcctgtagtttttttttattt
tgaatttgatctcttggaagGGATGCACATGTTAATGAAGAACGTTGCTGCTCGATGCCATTGAGTTAATAAGTTGAATCGACAGAATGGAGGTAATTACCGTGGAGAGTAGAGGACCGGACCGCAACAACAATATTTGCTGCAGCGCAGCCATAATAGGTATGTGATTTGTATTTATGTCATATTCATGGTTTTGAGTA
CATAGATTAAATTAGAGTTTTCTTGGCTAATTAGCAGATCGTCCTTCCTCATCAAAGCCCATGTCCCTCCCAATCAATCTGTCATTCCATCCTTCAATGTGATTTTTCATCCCCTGTTTTGATGAGATTTCCCCTTCTCTGTATTGATGGGGACACGGGACGAAAAATCGCCCCCTGCCTTAACGCCGCTTAACCTTACC
GCCACTTAGATTAATGCTCTTTTTAAAAACTTGGAATGGATAGGGGAGGCTCGATTCAAGCTTCAACCACAATTTCGAATCTATCGCAATTTGTTTTTTCAGGAATTCAAGGCGACCTCTACATAGGGACCCATTGTTCTGTACTTGGAGGATCGTTATTTACTAGCTAGATCTCGTTGGTCTATAGTTGGGTGTGATCA
TTGGTCCTTGGTTCTTCAAGTCATGGACCTACTCGATATTACTCCTCGCTTTCCCTTCCCCGCATTGTTTAGAGCCTTTGATCGCTGCTGAATTAGGGTTTAATAGCTAGATCAATCACATATAAGTAAGGCAAGATGTCCGGGTGCTTTCATTTCCCCTAATTGTTTAGAGCCATTGATCGCTGGTGAATTGGAATTTA
ATAGCTAGATAAATCATATACAAGGCAAGATGCTCAAACAGATAGATCATATATAAGTAACAAATAACAACGGTAAAACTATATCGAAATCTCTTCCCGAAATGCCGAAATGGAGAGCTTCACCCGTGTGGGAAACTAAGGTAGATCAGAAGATCCAACTGAAATAGTTGTCCAAGAGATCTTAAAAAACCAACTACCAT
GATCGGTAACGATATATATTATGGTATGGTATGGTATTGACGTCACGTCGATCTGAACCCAAGTTGGTTTTCTGAACCCAATAGTTGAAATCATAGTGGTATAGCTCGGAGAAATAAATAAGGGTTTGTTCCTTGCAATAAAACACGCCAAACCAACGTGGCCAGGTGTGGCCTAGACATGCTTTAGCAGAACTGTATCT
CCTTCGCCGTGATTTTTTACTTGACCGTCCAACTGTGAATGTATGTCACGGACGGAGAGAGTGGGGCGTCAGGGTTGGCTAGCGTCCTATTCTCGGGCTTTCATCCACCTATTTCAATGAAGGCACTTCGATCAACCTTTCGAATGATGCTACTTGCAAGCGGGCTGTCGAGTAAAGGGGCGGGTTTAGCACCTATGGAT
GTGAATGGATTTTCCCATTTTTATTCATCTGCTTAGGTCCAACCATCTTAGGACTGTATCTTTTCTTATCTTACTAGCAACCAATTCCATACTCCATTCAGTATAGTTTGGATAAGGGGCCTGAAATGAGGGAAGAGGATCAAGTGAAAGTGGTATAGAATTTATTTTTCCTATTTCCATCTGTTTATTGAGATTCTTTG
CATTACGGTTGGAGCTAGAATAGAGTTTTGTTTCCCGGTTTCCATCGGTTTATTGAGATTCTTTGCATTACGGTTAGAGCTGACCCATAGGAAAGAATAGGTTCTGCCTTCTGTTTACCAAGTTGTTGTACCTCCCCCAAAACTGCTATTATTCTTCCATTAACAACTAGAACCAACAATATGTTTCTAGGTGTCCCGGT
TGTCTCAGAATCCATCACGAGATCGAATTCCAAAAGAACACTAAACTTGAAGAAACTAAACGAGAAAGCCCACTGTCTCGCTCTCTATGTCGACACGCACTACATGGAGTTAAACATGTATTTATGATTTGGTAAGCCTGGTAGAGTTGGTTTTTGAATGTGC

Intron 2 GTAGTGACTC ... CTCTTGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.86	3	11	18
A2	76.47	2	5	8
A3	77.78	2	5	9
A4	69.23	2	3	6
A5	76	3	11	8
A6	40.43	14	5	14
A7	56.92	14	18	19
A8	86.05	3	19	18
A9	52.94	16	10	26
A10	43.21	23	14	21

>TRINITY_DN36017_c0_g1_i9 from sample A6 / Example spliced isoform

GGAAGGGTAGAAGTTGGTGTATATATATGTCTAGACGAATTATAATTGAATCCCTTCTGTTCACATCCTCCTCTTTTATCATTTTCATTTGCAGGGAATTTTCTTTTGTTTGCAGTGGAAGCGCTTCCTCCTGTTCATGCATGTCTGTGTGGGATGCACATGTTAATGAAGAACGTTGCTGCTCGATGCCATTGAGTTAA
TAAGTTGAATCGACAGAATGGAGGTAATTACCGTGGAGAGTAGAGGACCGGACCGCAACAACAATATTTGCTGCAGCGCAGCCATAATAGTTTGATTATGTTCTTTTTGGATGCGAAGGAAGGAAGACCAGCTTTAGATAGGATTCAATCCCACAGAAACCTTCTCTTAACAAGTATGTATGGATCGGGCTTCTCTGGCC
TTCTTTTTCAATAGTTTAACCCCG

>TRINITY_DN36017_c0_g1_i5 from sample A6 / Example unspliced isoform:

GGAAGGGTAGAAGTTGGTGTATATATATGTCTAGACGAATTATAATTGAATCCCTTCTGTTCACATCCTCCTCTTTTATCATTTTCATTTGCAGGGAATTTTCTTTTGTTTGCAGTGGAAGCGCTTCCTCCTGTTCATGCATGTCTGTGTGgtagtgactctgtgtcttgttcctgtttgtcggtattttgtttctgtgt
cgcgttgtgtgtctatctgtatcctgtagttttttttttattttgaatttgatctcttggaagGGATGCACATGTTAATGAAGAACGTTGCTGCTCGATGCCATTGAGTTAATAAGTTGAATCGACAGAATGGAGGTAATTACCGTGGAGAGTAGAGGACCGGACCGCAACAACAATATTTGCTGCAGCGCAGCCATAAT
AGTGCATCATTCAGCAAGAAAACACAGGTGTGGAGTCTCAATGGCCTTTAAGCGAAGTTAAGGGATGGTGCTCTCTAAACTGATGCCAACCGCTTTAGAGTGTGATTTGGGGATTTCGTGATGGATCTACATTACATGTGTCATATACAATAGTTGGCCGCATGCGTGTACTTATATGTTGGGAAAAAACGAATGCCTCT
TTTGAGTACAGCTTTGTATGCTAAAGATAAATTATAAAACC

Intron 3 GTGAGTCTGT ... CGACCAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	1	0	0
A2	33.33	1	0	1
A3	-1	0	0	0
A4	-1	0	0	0
A5	-1	0	0	0
A6	100	0	0	5
A7	87.18	5	33	35
A8	79.66	6	17	30
A9	88.24	6	38	52
A10	87.69	8	60	54

>TRINITY_DN36017_c0_g1_i1 from sample A6 / Example spliced isoform

GGAAGGGTAGAAGTTGGTGTATATATATGTCTAGACGAATTATAATTGAATCCCTTCTGTTCACATCCTCCTCTTTTATCATTTTCATTTGCAGGGAATTTTCTTTTGTTTGCAGTGGAAGCGCTTCCTCCCGTGGCTATGACGCTCTCTTTGTTTCCTCTGTGTGGTTGGCAGCAGGAAGGGATGCACGTGTTAATGGA
GAACGTTGCTGCTCGGATGCCATTGTGTTAATCATTTGAATCGACAGAATGGAGGTAATTACCCCAGAGAGCAGAGGACCGGACCGCAACAACAATATTTGCTGCAGCGCAGCCATAATAGTTTGATTATGTTCTTTTTGGATGCGAAGGAAGGAAGACCAGCTTTAGATAGGATTCAATCCCACAGAAACCTTCTCTTA
ACAAGTATGTATGGATCGGGCTTCTCTGGCCTTCTTTTTCAATAGTTTAACCCCG

>TRINITY_DN36017_c0_g1_i3 from sample A6 / Example unspliced isoform:

GGAAGGGTAGAAGTTGGTGTATATATATGTCTAGACGAATTATAATTGAATCCCTTCTGTTCACATCCTCCTCTTTTATCATTTTCATTTGCAGGGAATTTTCTTTTGTTTGCAGTGGAAGCGCTTCCTCCCGTGGCTATGACGCTCTCTTTGTTTCCTCTGTGTGGTTgtgagtctgtgactctgtgtctagttcctgt
gtgtctgtattgtgtgtctgtgtcgcgttctgtgtctatctgtatcctgtagttttttttttattttgaatttgatctcttggatctcgaccaaaagGGCAGCAGGAAGGGATGCACGTGTTAATGGAGAACGTTGCTGCTCGGATGCCATTGTGTTAATCATTTGAATCGACAGAATGGAGGTAATTACCCCAGAGAGC
AGAGGACCGGACCGCAACAACAATATTTGCTGCAGCGCAGCCATAATAGTTTGATTATGTTCTTTTTGGATGCGAAGGAAGGAAGACCAGCTTTAGATAGGATTCAATCCCACAGAAACCTTCTCTTAACAAGTATGTATGGATCGGGCTTCTCTGGCCTTCTTTTTCAATAGTTTAACCCCG