sp|P86071|TALDO_CAPAA ----> Transaldolase (Fragment)

Intron 1 GTTGAGGTGT ... AGTTCTTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.39	85	141	63
A2	70.63	47	113	35.5
A3	72.34	52	136	50
A4	70.54	35.5	85	30
A5	81.67	11	49	4
A6	72.27	49.5	129	30
A7	67.21	180	369	104
A8	58.61	104.5	148	48
A9	64.42	158	286	106
A10	67.26	147	302	87.5

>TRINITY_DN42776_c1_g2_i3 from sample A8 / Example spliced isoform

CTCTGTTTTCCGTCTTTACTCGAAGATCCGACCGTTTGTCGTTTATCCCTTGAGGTTTTCCACTCCTTTTTCAAAGATCTCCGCGTCTGCATTTCTCCAAGATATGAGCGTTTGTCTCGATCTTGCTTGCTGTGTTCGAACGTTAGATTATGGCAACCATAGCGCAATTGAAGTATGCCCAGGGTTCTTCTTTCACTAAT
GCTTATGCAAATTCTCTGGAGGGAAGGAAATCGGGGTTTGCCAATCAGGAATTCGTGGATTTCAAGGGGAATGTTTCAACCGTGCCTTTGTTTTCTAAAGCTAATCATTCCAGAGGTGATGGCGGAGCCCAGAAACTTTCGAGGCAACTTATCAACGGGGGAAAAGGAGTTGAGGACACATACTGGGAGCTTGTAATAAA
AGATATCCAAGATGCATGCGAATTACTTGAGCCCATTTATGATGCAACTGAAGGAGCAGATGGTTATGTTTCTGTTCAAGTCTCCCCCAGACTTGTGCTTGATACAGAAGGGACTGTTGAAGCTGCTAAGTGGCTGCACAAAAGAGTCAATCGCTGCAATGTCTACATTAAGATTCCAGCAACTTTAGAATGCATTCCAT
CTATAAAGCAAGTTGTTTCACTTGGTATCGGTGTCACTGTTACTCTTATATCCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAGTCTTTTAAGGGCGACGATCTTTCAAAAATTACAGGTGCTGCTGCCATTTATGTAAGTCGTGTTGACACGCTTCTAGATAATAAGCTTGAAAAGATT
GGAACTGATGATGCACTTGCCCTAAGAGGGAAGGCTGCCGTAGCACAAGCTGTTCTTGCATACAAAATATATCAGGAAAAGTTCTGTGGCCCTCGATGGGAAGCTCTTGAAAAAAGAGGCGCAAGAAAGCAAAGAGTAATGTGGGCCTCAACCAATGTAAAAAATCCAGCATATCAGGATACCCTATATGTTAATTCTTT
GATTGGCCCTGAAACTATCACGACAATGCCAGATTTTGCACTCCAGGCATTCATGGACCATGGGAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAAAGAATGTCTATAACACAATTGAAAAGATAGGAGTTGACTGGGCTGAAGTTGGCTCCTTACTGGAATTAGAAGGTGTGGAGTCTTTCAAGAAGAGCT
TTGATAGCGTTCTTGTTATTCTTGGTGGAAAAGCAGATCTGCTTAAAAGGGCAAATTCATAGATTAGGATTATTTATATTAGTCTGGAAAGCAGTGTTTACAGATGGTTGGTAAGCTAATGTCAACACAACTAAAATGACATCACACTGCAGAGGGATAAAATGTAAACCCATCAAAGTATATGGAGCTATTTTATTTGC
TTACCAGGAATAATTGTAAGAGTAGAGCAAAATATGCGGTACTTATATTGCAGGTTGTATTAGATGTTACCTATGAGGGCTGCTATGAGAATTACCTGCTTGCTTTCATCAGAGCTTATCCAACGAAATGAAGTATAAGAATTACCAGCTTGGTTTCGTCAAAGCTTATCCAGTGAAACAAAAAATACATTCATCATCAT
CATTCATTATCTAGGAAACAAGTTGTGATTACATTTCCACATTTTCATCCATTATCATAAAGTTTTGATGCCCGAGTTGTACACTGTACGATCAATTCATGAGATAAGAACTTTTTGAACAAGATTGCCAAAAGGAAACTTTTTGCCTTCTTACTACTTTTTTTTTCTTTTCTTTTTTTCAATGTCATCG

>TRINITY_DN42776_c1_g2_i2 from sample A8 / Example unspliced isoform:

CTCTGTTTTCCGTCTTTACTCGAAGATCCGACCGTTTGTCGTTTATCCCTTGAGGTTTTCCACTCCTTTTTCAAAGATCTCCGCGTCTGCATTTCTCCAAGATATGAGCGTTTGTCTCGATCTTGCTTGCTGTGTTCGAACGTTAGATTATGGCAACCATAGCGCAATTGAAGTATGCCCAGGGTTCTTCTTTCACTAAT
GCTTATGCAAATTCTCTGGAGGGAAGGAAATCGGGGTTTGCCAATCAGGAATTCGTGGATTTCAAGGGGAATGTTTCAACCGTGCCTTTGTTTTCTAAAGCTAATCATTCCAGAGGTGATGGCGGAGCCCAGAAACTTTCGAGgttgaggtgtgcagttgcagaaccaaaagctaaagtggaaaagaaaacaattctgca
tgagctttatgagcagcaggggcagagtccttattatgacaatctcaggcgtccagttagtgacttgaagccacttattgaaagtggagtccgaggcattacaagcaatcccacaattttggaaaaagctataatatcttcagatgcttatgatgaccagttcaggtattcgtaggcctctgtacagttattggatctac
atttgtctctctataaatcaattaccagttcttaagGCAACTTATCAACGGGGGAAAAGGAGTTGAGGACACATACTGGGAGCTTGTAATAAAAGATATCCAAGATGCATGCGAATTACTTGAGCCCATTTATGATGCAACTGAAGGAGCAGATGGTTATGTTTCTGTTCAAGTCTCCCCCAGACTTGTGCTTGATACAG
AAGGGACTGTTGAAGCTGCTAAGTGGCTGCACAAAAGAGTCAATCGCTGCAATGTCTACATTAAGATTCCAGCAACTTTAGAATGCATTCCATCTATAAAGCAAGTTGTTTCACTTGGTATCGGTGTCACTGTTACTCTTATATCCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAGTCT
TTTAAGGGCGACGATCTTTCAAAAATTACAGGTGCTGCTGCCATTTATGTAAGTCGTGTTGACACGCTTCTAGATAATAAGCTTGAAAAGATTGGAACTGATGATGCACTTGCCCTAAGAGGGAAGGCTGCCGTAGCACAAGCTGTTCTTGCATACAAAATATATCAGGAAAAGTTCTGTGGCCCTCGATGGGAAGCTCT
TGAAAAAAGAGGCGCAAGAAAGCAAAGAGTAATGTGGGCCTCAACCAATGTAAAAAATCCAGCATATCAGGATACCCTATATGTTAATTCTTTGATTGGCCCTGAAACTATCACGACAATGCCAGATTTTGCACTCCAGGCATTCATGGACCATGGGAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAAAGA
ATGTCTATAACACAATTGAAAAGATAGGAGTTGACTGGGCTGAAGTTGGCTCCTTACTGGAATTAGAAGGTGTGGAGTCTTTCAAGAAGAGCTTTGATAGCGTTCTTGTTATTCTTGGTGGAAAAGCAGATCTGCTTAAAAGGGCAAATTCATAGATTAGGATTATTTATATTAGTCTGGAAAGCAGTGTTTACAGATGG
TTGGTAAGCTAATGTCAACACAACTAAAATGACATCACACTGCAGAGGGATAAAATGTAAACCCATCAAAGTATATGGAGCTATTTTATTTGCTTACCAGGAATAATTGTAAGAGTAGAGCAAAATATGCGGTACTTATATTGCAGGTTGTATTAGATGTTACCTATGAGGGCTGCTATGAGAATTACCTGCTTGCTTTC
ATCAGAGCTTATCCAACGAAATGAAGTATAAGAATTACCAGCTTGGTTTCGTCAAAGCTTATCCAGTGAAACAAAAAATACATTCATCATCATCATTCATTATCTAGGAAACAAGTTGTGATTACATTTCCACATTTTCATCCATTATCATAAAGTTTTGATGCCCGAGTTGTACACTGTACGATCAATTCATGAGATAA
GAACTTTTTGAACAAGATTGCCAAAAGGAAACTTTTTGCCTTCTTACTACTTTTTTTTTCTTTTCTTTTTTTCAATGTCATCG

Intron 2 GTAAGTGGCA ... TTGTTTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.85	290	0	63
A2	8.67	316	0	30
A3	11.49	285	0	37
A4	14.29	204	0	34
A5	14.17	103	0	17
A6	10.47	710	0	83
A7	7.84	1387	0	118
A8	11.43	1279	0	165
A9	10.03	1139	0	127
A10	8.05	902	0	79

>TRINITY_DN42776_c1_g2_i1 from sample A8 / Example spliced isoform

TGATGATTGGCTGAGTTTGTGCATCTTCAAAGTCCCAAACTGTTATCCTTATTTTATAAAATTTTCAATGTATTTATCATGTTGCTAAATTTGCATGGTACTACAGCTTATATCCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAGTCTTTTAAGGGCGACGATCTTTCAAAAATTACAG
GTGCTGCTGCCATTTATGTAAGTCGTGTTGACACGCTTCTAGATAATAAGCTTGAAAAGATTGGAACTGATGATGCACTTGCCCTAAGAGGGAAGGCTGCCGTAGCACAAGCTGTTCTTGCATACAAAATATATCAGGAAAAGTTCTGTGGCCCTCGATGGGAAGCTCTTGAAAAAAGAGGCGCAAGAAAGCAAAGAGTA
ATGTGGGCCTCAACCAATGTAAAAAATCCAGCATATCAGGATACCCTATATGTTAATTCTTTGATTGGCCCTGAAACTATCACGACAATGCCAGATTTTGCACTCCAGGCATTCATGGACCATGGGAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAAAGAATGTCTATAACACAATTGAAAAGATAGGAGT
TGACTGGGCTGAAGTTGGCTCCTTACTGGAATTAGAAGGTGTGGAGTCTTTCAAGAAGAGCTTTGATAGCGTTCTTGTTATTCTTGGTGGAAAAGCAGATCTGCTTAAAAGGGCAAATTCATAGATTAGGATTATTTATATTAGTCTGGAAAGCAGTGTTTACAGATGGTTGGTAAGCTAATGTCAACACAACTAAAATG
ACATCACACTGCAGAGGGATAAAATGTAAACCCATCAAAGTATATGGAGCTATTTTATTTGCTTACCAGGAATAATTGTAAGAGTAGAGCAAAATATGCGGTACTTATATTGCAGGTTGTATTAGATGTTACCTATGAGGGCTGCTATGAGAATTACCTGCTTGCTTTCATCAGAGCTTATCCAACGAAATGAAGTATAA
GAATTACCAGCTTGGTTTCGTCAAAGCTTATCCAGTGAAACAAAAAATACATTCATCATCATCATTCATTATCTAGGAAACAAGTTGTGATTACATTTCCACATTTTCATCCATTATCATAAAGTTTTGATGCCCGAGTTGTACACTGTACGATCAATTCATGAGATAAGAACTTTTTGAACAAGATTGCCAAAAGGAAA
CTTTTTGCCTTCTTACTACTTTTTTTTTCTTTTCTTTTTTTCAATGTCATCG

>TRINITY_DN42776_c1_g2_i4 from sample A8 / Example unspliced isoform:

ATTCTTTGATTGGCCCTGAAACTgtaagtggcatctttgtaggttaggcaggtgcttttattgaataagatagtcatattctcttttatgagaaacaatttactccctttacttgacattaatgtcttaatatgtgttatgtcctttgtttctagATCACGACAATGCCAGATTTTGCACTCCAGGCATTCATGGACCAT
GGGAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAAAGAATGTCTATAACACAATTGAAAAGATAGGAGTTGACTGGGCTGAAGTTGGCTCCTTACTGGAATTAGAAGGTGTGGAGTCTTTCAAGAAGAGCTTTGATAGCGTTCTTGTTATTCTTGGTGGAAAAGCAGATCTGCTTAAAAGGGCAAATTCATA
GATTAGGATTATTTATATTAGTCTGGAAAGCAGTGTTTACAGATGGTTGGTAAGCTAATGTCAACACAACTAAAATGACATCACACTGCAGAGGGATAAAATGTAAACCCATCAAAGTATATGGAGCTATTTTATTTGCTTACCAGGAATAATTGTAAGAGTAGAGCAAAATATGCGGTACTTATATTGCAGGTTGTATT
AGATGTTACCTATGAGGGCTGCTATGAGAATTACCTGCTTGCTTTCATCAGAGCTTATCCAACGAAATGAAGTATAAGAATTACCAGCTTGGTTTCGTCAAAGCTTATCCAGTGAAACAAAAAATACATTCATCATCATCATTCATTATCTAGGAAACAAGTTGTGATTACATTTCCACATTTTCATCCATTATCATAAA
GTTTTGATGCCCGAGTTGTACACTGTACGATCAATTCATGAGATAAGAACTTTTTGAACAAGATTGCCAAAAGGAAACTTTTTGCCTTCTTACTACTTTTTTTTTCTTTTCTTTTTTTCAATGTCATCG

Intron 3 GTTGAGGTGT ... ACCAGTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.15	85	141	278.3
A2	77.13	47	113	204
A3	80.18	51.6	136	281.4
A4	81.47	35.7	85	229
A5	85.57	11	49	81.4
A6	78.55	49.7	129	235.1
A7	74.45	180	369	680.4
A8	69.75	104.7	148	334.9
A9	74.9	158.9	286	662
A10	75.26	147.4	302	595

>TRINITY_DN123913_c1_g2_i3 from sample A1 / Example spliced isoform

ATTGAGTTTATCCACTGCTATCTCACTCTGTTTTCCGTCTTTACTCGAAGATCCGACCGTTTGTCGTTTATCCCTTGAGGTTTTCCACTCCTTTTTCAAAGATCTCCGCGTCTGCATTTCTCCAAGATATGAGCGTTTGTCTCGATCTTGCTTGCTGTGTTCGAACGTTAGATTATGGCAACCATAGCGCAATTGAAGTA
TGCCCAGGGTTCTTCTTTCACTAATGCTTATGCAAATTCTCTGGAGGGAAGGAAATCGGGGTTTGCCAATCAGGAATTCGTGGATTTCAAGGGGAATGTTTCAACCGTGCCTTTGTTTTCTAAAGCTAATCATTCCAGAGGTGATGGCGGAGCCCAGAAACTTTCGAGGCAACTTATCAACGGGGGAAAAGGAGTTGAGG
ACACATACTGGGAGCTTGTAATAAAAGATATCCAAGATGCATGCGAATTATTTGAGCCCATTTATGATGCAACTGAAGGAGCAGATGGTTATGTTTCTGTTCAAGTCTCCCCCAGACTTGTGCTTGATACAGAAGGGACTGTTGAAGCTGCTAAGTGGCTGCACAAAAGAGTCAATCGCTGCAATGTCTACATTAAGATT
CCAGCAACTTTAGAATGCATTCCATCTATAAAGCAAGTTGTTTCACTTGGTATCGGTGTCACTGTTACTCTTATATCCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAGTCTTTTAAGGGCGACGATCTTTCAAAAATTACAGGTGCTGCTGCCATTTATGTAAGTCGTGTTGACACGCT
TCTAGATAATAAGCTTGAAAAGATTGGAACTGATGATGCACTTGCCCTAAGAGGGAAGGCTGCCGTAGCACAAGCTGTTCTTGCATACAAAATATATCAGGAAAAGTTCTGTGGCCCTCGATGGGAAGCTCTTGAAAAAAGAGGCGCAAGAAAGCAAAGAGTAATGTGGGCCTCAACCAATGTAAAAAATCCAGCATATC
AGGATACCCTATATGTTAATTCTTTGATTGGCCCTGAAACTATCACGACAATGCCAGATTTTGCACTCCAGGCATTCATGGACCATGGAAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAAAGAATGTCTATAACACAATTGAAAAGATAGGAGTTGACTGGGCTGAAGTTGGTTCCTTACTGGAATTAGAA
GGTGTGGAGTCTTTCAAGAAGAGCTTTGATAGCGTTCTTGTTATTCTTGGTGGAAAAGCAGATCTGCTTAAAAGGGCAAATTCATAGATTAGGATTATTTATATTAGTCTGGAAAGCAGTGTTTACAGATGGTTGGTAAGCTAATGTCAACACAACTAAAATGACATCACACTGCAGAGGGATAAAATGTAAACCCATCA
AAGTATATGGAGCTATTTTATTTGCTTACCAGGAATAATTGTAAGAGTAGAGCAAAATATGCGGTACTTATATTGCAGGTTGTATTAGATGTTACCTATGAGGGCTGCTATGAGAATTACCTGCTTGCTTTCATCAGAGCTTATCCAACGAAATGAAGTATAAGAATTACCAGCTTGGTTTCGTCAAAGCTTATCCAGTG
AAACAAAAAATACATTCATCATCATCATTCATTATCTAGGAAACAAGTTGTGATTACATTTCCACATTTTCATCCATTATCATAAAGTTTTGATGCCCGAGTTGTACACTGTACGATCAATTCATGAGATAAGAACTTTTTGAACAAGATTGCCAAAAG

>TRINITY_DN123913_c1_g2_i4 from sample A1 / Example unspliced isoform:

ATTGAGTTTATCCACTGCTATCTCACTCTGTTTTCCGTCTTTACTCGAAGATCCGACCGTTTGTCGTTTATCCCTTGAGGTTTTCCACTCCTTTTTCAAAGATCTCCGCGTCTGCATTTCTCCAAGATATGAGCGTTTGTCTCGATCTTGCTTGCTGTGTTCGAACGTTAGATTATGGCAACCATAGCGCAATTGAAGTA
TGCCCAGGGTTCTTCTTTCACTAATGCTTATGCAAATTCTCTGGAGGGAAGGAAATCGGGGTTTGCCAATCAGGAATTCGTGGATTTCAAGGGGAATGTTTCAACCGTGCCTTTGTTTTCTAAAGCTAATCATTCCAGAGGTGATGGCGGAGCCCAGAAACTTTCGAGgttgaggtgtgcagttgcagaaccaaaagcta
aagtggaaaagaaaacaattctgcatgagctttatgagcagcaggggcagagtccttattatgacaatctcaggcgtccagttagtgacttgaagccacttattgaaagtggagtccgaggcattacaagcaatcccacaattttcgaaaaagctataatatcttcagatgcttatgatgaccagttcagGCAACTTATC
AACGGGGGAAAAGGAGTTGAGGACACATACTGGGAGCTTGTAATAAAAGATATCCAAGATGCATGCGAATTATTTGAGCCCATTTATGATGCAACTGAAGGAGCAGATGGTTATGTTTCTGTTCAAGTCTCCCCCAGACTTGTGCTTGATACAGAAGGGACTGTTGAAGCTGCTAAGTGGCTGCACAAAAGAGTCAATCG
CTGCAATGTCTACATTAAGATTCCAGCAACTTTAGAATGCATTCCATCTATAAAGCAAGTTGTTTCACTTGGTATCGGTGTCACTGTTACTCTTATATCCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAGTCTTTTAAGGGCGACGATCTTTCAAAAATTACAGGTGCTGCTGCCATTT
ATGTAAGTCGTGTTGACACGCTTCTAGATAATAAGCTTGAAAAGATTGGAACTGATGATGCACTTGCCCTAAGAGGGAAGGCTGCCGTAGCACAAGCTGTTCTTGCATACAAAATATATCAGGAAAAGTTCTGTGGCCCTCGATGGGAAGCTCTTGAAAAAAGAGGCGCAAGAAAGCAAAGAGTAATGTGGGCCTCAACC
AATGTAAAAAATCCAGCATATCAGGATACCCTATATGTTAATTCTTTGATTGGCCCTGAAACTATCACGACAATGCCAGATTTTGCACTCCAGGCATTCATGGACCATGGAAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAAAGAATGTCTATAACACAATTGAAAAGATAGGAGTTGACTGGGCTGAAGT
TGGTTCCTTACTGGAATTAGAAGGTGTGGAGTCTTTCAAGAAGAGCTTTGATAGCGTTCTTGTTATTCTTGGTGGAAAAGCAGATCTGCTTAAAAGGGCAAATTCATAGATTAGGATTATTTATATTAGTCTGGAAAGCAGTGTTTACAGATGGTTGGTAAGCTAATGTCAACACAACTAAAATGACATCACACTGCAGA
GGGATAAAATGTAAACCCATCAAAGTATATGGAGCTATTTTATTTGCTTACCAGGAATAATTGTAAGAGTAGAGCAAAATATGCGGTACTTATATTGCAGGTTGTATTAGATGTTACCTATGAGGGCTGCTATGAGAATTACCTGCTTGCTTTCATCAGAGCTTATCCAACGAAATGAAGTATAAGAATTACCAGCTTGG
TTTCGTCAAAGCTTATCCAGTGAAACAAAAAATACATTCATCATCATCATTCATTATCTAGGAAACAAGTTGTGATTACATTTCCACATTTTCATCCATTATCATAAAGTTTTGATGCCCGAGTTGTACACTGTACGATCAATTCATGAGATAAGAACTTTTTGAACAAGATTGCCAAAAG

Intron 4 GTATTCGTAG ... AGTTCTTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.88	277.5	34	63
A2	17.82	204	53	35.5
A3	15	280.5	49	50
A4	14.25	228.5	46	30
A5	14.29	81	23	4
A6	16.99	234.5	66	30
A7	15.57	678	146	104
A8	16.25	332.5	81	48
A9	16.57	659.5	156	106
A10	16.93	592.5	154	87.5

>TRINITY_DN42776_c1_g2_i5 from sample A8 / Example spliced isoform

CTCTGTTTTCCGTCTTTACTCGAAGATCCGACCGTTTGTCGTTTATCCCTTGAGGTTTTCCACTCCTTTTTCAAAGATCTCCGCGTCTGCATTTCTCCAAGATATGAGCGTTTGTCTCGATCTTGCTTGCTGTGTTCGAACGTTAGATTATGGCAACCATAGCGCAATTGAAGTATGCCCAGGGTTCTTCTTTCACTAAT
GCTTATGCAAATTCTCTGGAGGGAAGGAAATCGGGGTTTGCCAATCAGGAATTCGTGGATTTCAAGGGGAATGTTTCAACCGTGCCTTTGTTTTCTAAAGCTAATCATTCCAGAGGTGATGGCGGAGCCCAGAAACTTTCGAGGTTGAGGTGTGCAGTTGCAGAACCAAAAGCTAAAGTGGAAAAGAAAACAATTCTGCA
TGAGCTTTATGAGCAGCAGGGGCAGAGTCCTTATTATGACAATCTCAGGCGTCCAGTTAGTGACTTGAAGCCACTTATTGAAAGTGGAGTCCGAGGCATTACAAGCAATCCCACAATTTTGGAAAAAGCTATAATATCTTCAGATGCTTATGATGACCAGTTCAGGCAACTTATCAACGGGGGAAAAGGAGTTGAGGACA
CATACTGGGAGCTTGTAATAAAAGATATCCAAGATGCATGCGAATTACTTGAGCCCATTTATGATGCAACTGAAGGAGCAGATGGTTATGTTTCTGTTCAAGTCTCCCCCAGACTTGTGCTTGATACAGAAGGGACTGTTGAAGCTGCTAAGTGGCTGCACAAAAGAGTCAATCGCTGCAATGTCTACATTAAGATTCCA
GCAACTTTAGAATGCATTCCATCTATAAAGCAAGTTGTTTCACTTGGTATCGGTGTCACTGTTACTCTTATATCCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAGTCTTTTAAGGGCGACGATCTTTCAAAAATTACAGGTGCTGCTGCCATTTATGTAAGTCGTGTTGACACGCTTCT
AGATAATAAGCTTGAAAAGATTGGAACTGATGATGCACTTGCCCTAAGAGGGAAGGCTGCCGTAGCACAAGCTGTTCTTGCATACAAAATATATCAGGAAAAGTTCTGTGGCCCTCGATGGGAAGCTCTTGAAAAAAGAGGCGCAAGAAAGCAAAGAGTAATGTGGGCCTCAACCAATGTAAAAAATCCAGCATATCAGG
ATACCCTATATGTTAATTCTTTGATTGGCCCTGAAACTATCACGACAATGCCAGATTTTGCACTCCAGGCATTCATGGACCATGGGAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAAAGAATGTCTATAACACAATTGAAAAGATAGGAGTTGACTGGGCTGAAGTTGGCTCCTTACTGGAATTAGAAGGT
GTGGAGTCTTTCAAGAAGAGCTTTGATAGCGTTCTTGTTATTCTTGGTGGAAAAGCAGATCTGCTTAAAAGGGCAAATTCATAGATTAGGATTATTTATATTAGTCTGGAAAGCAGTGTTTACAGATGGTTGGTAAGCTAATGTCAACACAACTAAAATGACATCACACTGCAGAGGGATAAAATGTAAACCCATCAAAG
TATATGGAGCTATTTTATTTGCTTACCAGGAATAATTGTAAGAGTAGAGCAAAATATGCGGTACTTATATTGCAGGTTGTATTAGATGTTACCTATGAGGGCTGCTATGAGAATTACCTGCTTGCTTTCATCAGAGCTTATCCAACGAAATGAAGTATAAGAATTACCAGCTTGGTTTCGTCAAAGCTTATCCAGTGAAA
CAAAAAATACATTCATCATCATCATTCATTATCTAGGAAACAAGTTGTGATTACATTTCCACATTTTCATCCATTATCATAAAGTTTTGATGCCCGAGTTGTACACTGTACGATCAATTCATGAGATAAGAACTTTTTGAACAAGATTGCCAAAAGGAAACTTTTTGCCTTCTTACTACTTTTTTTTTCTTTTCTTTTTT
TCAATGTCATCG

>TRINITY_DN42776_c1_g2_i2 from sample A8 / Example unspliced isoform:

CTCTGTTTTCCGTCTTTACTCGAAGATCCGACCGTTTGTCGTTTATCCCTTGAGGTTTTCCACTCCTTTTTCAAAGATCTCCGCGTCTGCATTTCTCCAAGATATGAGCGTTTGTCTCGATCTTGCTTGCTGTGTTCGAACGTTAGATTATGGCAACCATAGCGCAATTGAAGTATGCCCAGGGTTCTTCTTTCACTAAT
GCTTATGCAAATTCTCTGGAGGGAAGGAAATCGGGGTTTGCCAATCAGGAATTCGTGGATTTCAAGGGGAATGTTTCAACCGTGCCTTTGTTTTCTAAAGCTAATCATTCCAGAGGTGATGGCGGAGCCCAGAAACTTTCGAGGTTGAGGTGTGCAGTTGCAGAACCAAAAGCTAAAGTGGAAAAGAAAACAATTCTGCA
TGAGCTTTATGAGCAGCAGGGGCAGAGTCCTTATTATGACAATCTCAGGCGTCCAGTTAGTGACTTGAAGCCACTTATTGAAAGTGGAGTCCGAGGCATTACAAGCAATCCCACAATTTTGGAAAAAGCTATAATATCTTCAGATGCTTATGATGACCAGTTCAGgtattcgtaggcctctgtacagttattggatctac
atttgtctctctataaatcaattaccagttcttaagGCAACTTATCAACGGGGGAAAAGGAGTTGAGGACACATACTGGGAGCTTGTAATAAAAGATATCCAAGATGCATGCGAATTACTTGAGCCCATTTATGATGCAACTGAAGGAGCAGATGGTTATGTTTCTGTTCAAGTCTCCCCCAGACTTGTGCTTGATACAG
AAGGGACTGTTGAAGCTGCTAAGTGGCTGCACAAAAGAGTCAATCGCTGCAATGTCTACATTAAGATTCCAGCAACTTTAGAATGCATTCCATCTATAAAGCAAGTTGTTTCACTTGGTATCGGTGTCACTGTTACTCTTATATCCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAGTCT
TTTAAGGGCGACGATCTTTCAAAAATTACAGGTGCTGCTGCCATTTATGTAAGTCGTGTTGACACGCTTCTAGATAATAAGCTTGAAAAGATTGGAACTGATGATGCACTTGCCCTAAGAGGGAAGGCTGCCGTAGCACAAGCTGTTCTTGCATACAAAATATATCAGGAAAAGTTCTGTGGCCCTCGATGGGAAGCTCT
TGAAAAAAGAGGCGCAAGAAAGCAAAGAGTAATGTGGGCCTCAACCAATGTAAAAAATCCAGCATATCAGGATACCCTATATGTTAATTCTTTGATTGGCCCTGAAACTATCACGACAATGCCAGATTTTGCACTCCAGGCATTCATGGACCATGGGAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAAAGA
ATGTCTATAACACAATTGAAAAGATAGGAGTTGACTGGGCTGAAGTTGGCTCCTTACTGGAATTAGAAGGTGTGGAGTCTTTCAAGAAGAGCTTTGATAGCGTTCTTGTTATTCTTGGTGGAAAAGCAGATCTGCTTAAAAGGGCAAATTCATAGATTAGGATTATTTATATTAGTCTGGAAAGCAGTGTTTACAGATGG
TTGGTAAGCTAATGTCAACACAACTAAAATGACATCACACTGCAGAGGGATAAAATGTAAACCCATCAAAGTATATGGAGCTATTTTATTTGCTTACCAGGAATAATTGTAAGAGTAGAGCAAAATATGCGGTACTTATATTGCAGGTTGTATTAGATGTTACCTATGAGGGCTGCTATGAGAATTACCTGCTTGCTTTC
ATCAGAGCTTATCCAACGAAATGAAGTATAAGAATTACCAGCTTGGTTTCGTCAAAGCTTATCCAGTGAAACAAAAAATACATTCATCATCATCATTCATTATCTAGGAAACAAGTTGTGATTACATTTCCACATTTTCATCCATTATCATAAAGTTTTGATGCCCGAGTTGTACACTGTACGATCAATTCATGAGATAA
GAACTTTTTGAACAAGATTGCCAAAAGGAAACTTTTTGCCTTCTTACTACTTTTTTTTTCTTTTCTTTTTTTCAATGTCATCG

Intron 5 GTACAGCAAA ... TGTTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.43	181.5	3	72
A2	25.44	164.5	5	53
A3	27.91	201	2	72
A4	31.02	178.5	2	75
A5	23.31	84.5	0	24
A6	22.32	197	0	53
A7	27.88	505.5	0	177
A8	25.14	276	0	86
A9	26.66	483	0	161
A10	26.04	466.5	0	152

>TRINITY_DN123913_c1_g2_i5 from sample A1 / Example spliced isoform

GTTAAATTATTTGGCAGAGAAATCCAAGCTACCCATTGTTTTGGCCAATTCCTATACATACTAGACTCCAAATCCCACATCCTTCTCATAGTATTTCTCAGCGATCTCCGCGTCTGTGATGCATTTCTCGAAGATCTGAGCGTTTGTCGTGTAATTCTCTGATATTGCCGTGTTTTACTTTCGGTTGGGTTTGAACGTTC
GACCATGGCAACCATAGCGCAATTGAGGCATGCCCAGATGTCTTCTTCCACTATTGCTAATGCAAAAGCGCTGGAGGGAAGGAAATCGGGGTTCGCCAATAGGGGATTCGTGGATTTCCAGGGGAAATTTTCAGTTCTGCCATTGTTTGCTAAAACTAACTTTTCTAGAAGGGATGGCGGAGCCCCAAAGCTTTCGGGGT
TGAGGTGTGCACTTGTAGAACCAAAGACTAAAGTTGAAAAGAAAACAATTCTGCATGAGCTTTATGAGCAGCAGGGGCAGAGTCCTTGGTATGACAATCTCAGGCGTCCAGTTAGTGACTTGAAACCACTCATTGAAAGTGGAGTCCGAGGAATTACAAGCAATCCTACGATTTTCGAAAAAGCTATAATATCTTCAGAT
GCTTATGATGACCAGTTCAGGCAACTTATCAAGGAGGGAAAAGGAGTTGAGGACACTTACTGGGAGCTTGTAATTAAAGATATCCAAGATGCATGCGAATTATTTGAGCCCATTTATGATGCAACTGAAGGAGCAGATGGTTATGTTTCTGTTGAAGTCTCCCCCAGACTTGCGCTTGAGACAGAAGGGACTGTTGAAGC
TGCTAAGTGGCTGCACAAAAGAGTCAATCGTCGCAATGTCTACATTAAGATTCCAGCTACGGCAGAATGTATTCCATCCATAAAGCAAGTTATTTCTCTTGGCATCAGTGTCAATGTCACTCTTATATTCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAATCTTTCAAGGGTGACGATC
TTTCGAAAATCACTAGTGTTGCTTCCTTTTTTGTAAGTCGCGTTGACACGCTTGTAGACAAAAAGCTTGAAAAGATTGGGACTGATGATGCACTTGCCCTAAGAGGGAAGGCTGCCACAGCACAAGCTGTTCTTGCATTCAAACTATATCAGGAAAAGTTCTCTGGCCCTCGATGGGAAGCGCTTGAAAAAAGAGGGGCA
AGAAAGCAAAGAGTATTGTGGGCCTCAACTAGTGTAAAAAATCCAGCATATCCGGATACACTATATGTTAATCCTCTGATTGGCCCTGAAACTGTCACGACAATGCCAGATCTTGCACTCCAGGCATTCATGGACCATGGAAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAGAGAATGTCTATAATGCAAT
TGAAAAAATAGGAATTGACTGGGCTGAAGTTGGCTCCTTACTGGAATTGGAAGGTGTTGAGTCTTTCAAGAAAAGCTTTGATAGCCTTCTTGTTAGTCTTGGTGGAAAAGCAGATCTGCTTAAAAAGGCAAATTCATAGATTAGTGTCTTAGTTATATCTGTCTTGACAGCAGTGTGTGCAGATTGTTGGTAAGGGACTG
TCAACAGAAGTAAGATGGCATCATACTGCAGAGGGATGAATTGTAATTCTATAGAAGTATATGGAGCTGTTTCATTAGCTTACTAGGAATAATTGTAAGAGTAGACCAAAACATGCAGTACTTATATTTCATGATGTATTGGATGTTACATGTCAGGGCTGCTATGAGGACTACCAGCTTCCTTTCTTCAAAGCTTATCC
AACGAAATGAAATATGAGAATTACCAGCTTGCTTTCATCAAAGCTTGTCCAACGAAACAAAATATGAGAATTACCAGCTTGGTCTCATCAAAGCTTAAATGAAAAATACATTCAACACCATCATTATCTAAGAAACAAAATCTGATTTCATTTTAAAATATACATCCACTACCATAAGCTTTTGATGCCCGAGTTGTGCA
CTCTATAATCAATTCATGAGATAAGAATTTCATCATTTTTTTAATAAGATTGCCGTTGAGAAATTTTCCATGTTACCAAATTCTTCAACTTTTTTCTTCAACTACTAATTAAATCACCATTTTCGGGGATCATTTTTTTGAAAAATTTGTTGAAAAT

>TRINITY_DN123913_c1_g2_i6 from sample A1 / Example unspliced isoform:

GGTATGACAATCTCAGGCGTCCAGTTAGTGACTTGAAACCACTCATTGAAAGTGGAGTCCGAGGAATTACAAGCAATCCTACGgtacagcaaacaacacatttactaaattgtattggggcttctactaaaattttagattcacatatatctttatttctattaaacttatttatattttgtacaactgaagctgggaaa
gagtctacagaggtcactgaaaactaaattagattgattttgactctgttatgcagATTTTCGAAAAAGCTATAATATCTTCAAATGCTTATGATGACCAGTTCAGGCAACTTATCAAGGAGGGAAAAGGAGTTGAGGACACTTACTGGGAGCTTGTAATTAAAGATATCCAAGATGCATGCGAATTATTTGAGCCCATT
TATGATGCAACTGAAGGAGCAGATGGTTATGTTTCTGTTGAAGTCTCCCCCAGACTTGCGCTTGAGACAGAAGGGACTGTTGAAGCTGCTAAGTGGCTGCACAAAAGAGTCAATCGTCGCAATGTCTACATTAAGATTCCAGCTACGGCAGAATGTATTCCATCCATAAAGCAAGTTATTTCTCTTGGCATCAGTGTCAA
TGTCACTCTTATATTCTCACTTCCACGATATGAAGCTGTGATTGATGCATACATGGAAGGTCTAGAATCTTTCAAGGGTGACGATCTTTCGAAAATCACTAGTGTTGCTTCCTTTTTTGTAAGTCGCGTTGACACGCTTGTAGACAAAAAGCTTGAAAAGATTGGGACTGATGATGCACTTGCCCTAAGAGGGAAGGCTG
CCACAGCACAAGCTGTTCTTGCATTCAAACTATATCAGGAAAAGTTCTCTGGCCCTCGATGGGAAGCGCTTGAAAAAAGAGGGGCAAGAAAGCAAAGAGTATTGTGGGCCTCAACTAGTGTAAAAAATCCAGCATATCCGGATACACTATATGTTAATCCTCTGATTGGCCCTGAAACTGTCACGACAATGCCAGATCTT
GCACTCCAGGCATTCATGGACCATGGAAAGGTTTCTAGAACTATTGATGCAAATTTTGAAGAAGCAGAGAATGTCTATAATGCAATTGAAAAAATAGGAATTGACTGGGCTGAAGTTGGCTCCTTACTGGAATTGGAAGGTGTTGAGTCTTTCAAGAAAAGCTTTGATAGCCTTCTTGTTAGTCTTGGTGGAAAAGCAGA
TCTGCTTAAAAAGGCAAATTCATAGATTAGTGTCTTAGTTATATCTGTCTTGACAGCAGTGTGTGCAGATTGTTGGTAAGGGACTGTCAACAGAAGTAAGATGGCATCATACTGCAGAGGGATGAATTGTAATTCTATAGAAGTATATGGAGCTGTTTCATTAGCTTACTAGGAATAATTGTAAGAGTAGACCAAAACAT
GCAGTACTTATATTTCATGATGTATTGGATGTTACATGTCAGGGCTGCTATGAGGACTACCAGCTTCCTTTCTTCAAAGCTTATCCAACGAAATGAAATATGAGAATTACCAGCTTGCTTTCATCAAAGCTTGTCCAACGAAACAAAATATGAGAATTACCAGCTTGGTCTCATCAAAGCTTAAATGAAAAATACATTCA
ACACCATCATTATCTAAGAAACAAAATCTGATTTCATTTTAAAATATACATCCACTACCATAAGCTTTTGATGCCCGAGTTGTGCACTCTATAATCAATTCATGAGATAAGAATTTCATCATTTTTTTAATAAGATTGCCGTTGAGAAATTTTCCATGTTACCAAATTCTTCAACTTTTTTCTTCAACTACTAATTAAAT
CACCATTTTCGGGGATCATTTTTTTGAAAAATTTGTTGAAAAT