NA ----> NA

Intron 1 GTTTGCTTCC ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.16	17.5	6	8
A2	30	19	8	8
A3	34.66	33	21	14
A4	27.05	17.5	4	9
A5	32.29	10.5	9	1
A6	36.85	134.5	74	81
A7	34.07	168.5	97.5	75
A8	34.38	94.5	63	34
A9	45.17	84.5	68.5	67
A10	30.79	216.5	114.5	76

>TRINITY_DN104113_c0_g3_i3 from sample A3 / Example spliced isoform

CGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGACGGCGGGTGTGGGGTAGCGA
AGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAGTGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAAGTGAGTAATACGGTGTTTTAAAACCCAGTTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACC
AGTCCCGGAAACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCG
CCCAAGAAAACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTT
TCCCTGACCTATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGAT
TTGGATTCTGGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCA

>TRINITY_DN104113_c0_g3_i4 from sample A3 / Example unspliced isoform:

CGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGACGGCGGGTGTGGGGTAGCGA
AGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAGTGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAAGTGAGTAATACGGTGTTTTAAAACCCAGgtttgcttccaaaatggcgatcttagtttcattttcatgtaaatagtttgaaggcaagctgc
tgatttacattgatggttgtttgcagTTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGAAACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCT
CAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAAACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCC
TTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAG
CGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCTGGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCA

Intron 2 GTGAGTAATA ... TAAAACCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.84	26.5	24.3	19.3
A2	43.05	28.3	22.5	20.5
A3	46	37	29.5	33.5
A4	45.3	22.5	18.8	18.8
A5	45.13	15	13.8	10.8
A6	41.63	205.8	151	144.3
A7	45.05	232.3	202.8	178.8
A8	43.52	146.8	124.8	102.8
A9	37.8	194.3	144.3	93.8
A10	45.73	279.3	241.5	230.3

>TRINITY_DN102140_c0_g1_i4 from sample A2 / Example spliced isoform

GGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGACGGCGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACC
GACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAATTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGAAACCCCAGGAGATTGTGCCTGACACAATCTCTTTC
GTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAAACCACTTCGGAGATTTTCGCTAAGCGATTCAACAA
CAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAACAATATCGACGGGGAAGGTGAAATGCAA
GACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCTGGAACAATACAGGTTTGATTACTGTTACTTGTATC
ATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCAT

>TRINITY_DN102140_c0_g1_i1 from sample A2 / Example unspliced isoform:

GGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGACGGCGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACC
GACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAAgtgagtaatacggtgttttaaaacccagTTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGAAACCCCA
GGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAAACCACTT
CGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAA
CAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCTGGAACAA
TACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCAT

Intron 3 GTGAGTAATA ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.79	26	11	8
A2	27.67	27.5	13	8
A3	35.76	36	26	14
A4	30.07	22	10	9
A5	32.02	14	12	1
A6	32.81	200.5	114.5	81
A7	33.8	222.5	151	75
A8	30.56	140.5	89	34
A9	34.56	190.5	134	67
A10	30.41	267	156	76

>TRINITY_DN104113_c0_g3_i5 from sample A3 / Example spliced isoform

CGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGACGGCGGGTGTGGGGTAGCGA
AGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAGTGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAATTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGAAACCCCAGGAGATTGTGCC
TGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAAACCACTTCGGAGATTTTCG
CTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAACAATATCGACGG
GGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCTGGAACAATACAGGTTTGAT
TACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCA

>TRINITY_DN104113_c0_g3_i4 from sample A3 / Example unspliced isoform:

CGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGACGGCGGGTGTGGGGTAGCGA
AGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAGTGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAAgtgagtaatacggtgttttaaaacccaggtttgcttccaaaatggcgatcttagtttcattttcatgtaaatagtttgaaggcaagctgc
tgatttacattgatggttgtttgcagTTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGAAACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCT
CAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAAACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCC
TTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAG
CGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCTGGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCA