NA ----> NA

Intron 1 GTTGCCAACA ... CCTTTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.46	138	44	27
A2	21.29	98	39	14
A3	20.78	101	39	14
A4	21.13	56	23	7
A5	24.27	39	17	8
A6	19.32	71	22	12
A7	23.38	213	90	40
A8	22.62	183	76	31
A9	20	246	85	38
A10	21.57	180	62	37

>TRINITY_DN47197_c0_g1_i5 from sample A7 / Example spliced isoform

TTTTTTCTGATTTCTTTTCATGCACTCCGCATTTTTTTCCTTGTACCGTGTCCATCCTGTCCATCTCCAATTAATTTTAGAGACATCAATGGAGCATCGGGCTTCATTCCGAATCTTGTTTCAGACACTTTTCTATTAATGGAAATATCCCCACTTTCTACCCACAGGTTTCGAAAGGTGTATCATCATTTGTTGAACCG
ATAGCTTAGTGTCTCTGAACAGCACTTGCACAGTCAATTCGAGTGACAAACATGGAGCCACCAGGAAGTAAAGATGTTTTATTTGATTCTGATTTTGAGTCGATGGAGCAAAGTAAGTCAGATAGAAAAGTCCAGGACTCTCTGTTGAAGTTTGTTGCAGATGCAGTTGGAAACAAGCTTCTTATTGCATTTTCAAGATT
TGGAAAGCATCCCGTGGAGTGGGGTCACTGGCTCGATGTTTTAGATGACGAGAATCTGAAAAGATTCTGCAAAGATGTGAATGAAGAAGAAATTCTTACAGATGAGAGTGTACAGGAAACAAATACAGCTGTTGATCATTCCTCTTCAGATTCTGATATTGAGGGCCCTAGTCCAATTGATGGCAGCCACTCTCTTATGA
TTGCACAAGGTGTAGCTGCGTATGCATCTGCTGCTGCAAAGTATAATTGTCTTGCTGAATGGCCTTTGCTGGGACCCTTTGACATACAGATGCTGACATGTGAGAAATGCTCTCAGCATTTCTTCTCTTCATTTAACTACCGAAGGCACACACAAACACATCGTCATGTTGTGAAGAGTAAAAAGGAGAACTTAGTGAAT
GAGAGTGCTGACTTGAGGGGATACTGGGATAAGCTCCAGCCAGACAAGGCCAGAGACATAGTTTATTTTGGCAACATAATGTTTGAGGGGATTCCCGGAGACTCAGTTATAAAGTATTTGACATCTGTTGTTTGCCATTCCTGCATTCTTCCCCTGCCAAAAAGTGTTTTCAAGGCCGGGAACTCCCTTCTGGAATTGTT
ACAAACCAAGTCCTCCAAGTTTCCATGCACATCAGAAGAACTATTTCACACACTGAATCTTGCAAGTGAGAGGACATTTTTGCAAACCAGAGCATCTGGTTCCTTGCAGAAATTTATTTTTGAGGGAGAAGAAGGGAAGTTAGGACACAAAATCAAGAATCTGGTTGCTTCCATAGGCTTCCTGGTTGAGCAGAGACTGG
TTAAGGCATGGATTACTGAGAAAGATGTAGAGTCCCTTCGCTTGCAAAAGAAGCTTATAGATGAAGAGGAAGCAGTGCAGAGAAGGAAGGCAGAGGAACGTGAGAGAAAGCTTTTGAATCAAGAGAAAAGAAAAGAACTGAATAAAAAGGATAGCACAGATGTGAGCAGTGAGGATTCCAACAAGTTCCTTGAAGTTGGG
CTGAAAGAATTGAAAGATATTACGGCTGTCATTGACGAGAATTCTGATCAGCCCCTTGCCGATACAAAAGATGCTTCTTTAAATAAAGAGAAATCTTCTTCTGAAATTGCTTATGAAGCGAAATCTTCTTCCGAAATTACTTGTGAAGTGCACGATGCAGGTCACACAGGAGATATTGGCACTGGTATGGAGCATGCGCT
TCCTCAAGCCCTATAAGTTCATTCTTTTCTTCAACACAGAAAACCAGTCAATACAGAAAATATGCCTTCACATAAAGATGAGTCCACTGTTGAAGATGCTTATGAAGCAGAAGATGCAAGTCATACAGGAGATATTGTCACAGCAAAGCACAAGGCCTCTAACTCGTCTCCAGAAGTTCATTGTAATCCCCAGCACAAAG
AACCAGCGGATGCAGAAACATTGTCTTCACATGAAGATACAGGTCATACGAGAGACATTGATACAGCATTGGAGCATAGCGCTTCTCGGCGCTCGCTCATTCCCGGAAGTTCATTTTAATCCCAACCATCGAAAGTCGGTCCATAAATAAGATTTTATCTTCACAGTCAGTTAATCCCCAGTTGAAGGAGTCTGAAACTT
CATATGGAGATAAATCTGTAATTGGGAGGGATGTCAATAGGGGTGCAGCTTTTTTAACTCATTTTGTGTAATTTAATTCTCCATGTTCCAGAGCTTTTTTTTCTTCTAAATTTTTATCACATATATTTCGGCGTGTTAGAAACGATACTAGTCTTCGGTTGAAGCTAAAATGATGCCACATTAACCACAAAGTTCCAAGT
TGTCTAGCAATAGTGTATTTTAGTGTATTTTTGATTGGGACCAATGGGTGAACATTGATGTGACTTAAAATGATTAAAATGAGATTTCAAATTTCAGAAAAAAAAA

>TRINITY_DN47197_c0_g1_i9 from sample A7 / Example unspliced isoform:

TTTTTTCTGATTTCTTTTCATGCACTCCGCATTTTTTTCCTTGTACCGTGTCCATCCTGTCCATCTCCAATTAATTTTAGAGACATCAATGGAGCATCGGGCTTCATTCCGAATCTTGTTTCAGACACTTTTCTATTAATGGAAATATCCCCACTTTCTACCCACAGGTTTCGAAAGGTGTATCATCATTTGTTGAACCG
ATAGCTTAGTGTCTCTGAACAGCACTTGCACAGTCAATTCGAGTGACAAACATGGAGCCACCAGGAAGTAAAGATGTTTTATTTGATTCTGATTTTGAGTCGATGGAGCAAAGTAAGTCAGATAGAAAAGTCCAGGACTCTCTGTTGAAGTTTGTTGCAGATGCAGTTGGAAACAAGCTTCTTATTGCATTTTCAAGATT
TGGAAAGCATCCCGTGGAGTGGGGTCACTGGCTCGATGTTTTAGATGACGAGAATCTGAAAAGATTCTGCAAAGATGTGAATGAAGAAGAAATTCTTACAGATGAGAGTGTACAGGAAACAAATACAGCTGTTGATCATTCCTCTTCAGATTCTGATATTGAGGGCCCTAGTCCAATTGATGGCAGCCACTCTCTTATGA
TTGCACAAGGTGTAGCTGCGTATGCATCTGCTGCTGCAAAGTATAATTGTCTTGCTGAATGGCCTTTGCTGGGACCCTTTGACATACAGATGCTGACATGTGAGAAATGCTCTCAGCATTTCTTCTCTTCATTTAACTACCGAAGGCACACACAAACACATCGTCATGTTGTGAAGAGTAAAAAGgttgccaacataaaa
ttttctcaacagttttagtatgcagcatgatccctgatttcttttgattgactaccataaatctttaatttcaattttcatgttgttcaacatcagcgtatgcaactagctgtgcatatcatttgtttggttttttataattttgaggttgattgtgcacttgcttctctaatcttcaacctttcacagGAGAACTTAGT
GAATGAGAGTGCTGACTTGAGGGGATACTGGGATAAGCTCCAGCCAGACAAGGCCAGAGACATAGTTTATTTTGGCAACATAATGTTTGAGGGGATTCCCGGAGACTCAGTTATAAAGTATTTGACATCTGTTGTTTGCCATTCCTGCATTCTTCCCCTGCCAAAAAGTGTTTTCAAGGCCGGGAACTCCCTTCTGGAAT
TGTTACAAACCAAGTCCTCCAAGTTTCCATGCACATCAGAAGAACTATTTCACACACTGAATCTTGCAAGTGAGAGGACATTTTTGCAAACCAGAGCATCTGGTTCCTTGCAGAAATTTATTTTTGAGGGAGAAGAAGGGAAGTTAGGACACAAAATCAAGAATCTGGTTGCTTCCATAGGCTTCCTGGTTGAGCAGAGA
CTGGTTAAGGCATGGATTACTGAGAAAGATGTAGAGTCCCTTCGCTTGCAAAAGAAGCTTATAGATGAAGAGGAAGCAGTGCAGAGAAGGAAGGCAGAGGAACGTGAGAGAAAGCTTTTGAATCAAGAGAAAAGAAAAGAACTGAATAAAAAGGATAGCACAGATGTGAGCAGTGAGGATTCCAACAAGTTCCTTGAAGT
TGGGCTGAAAGAATTGAAAGATATTACGGCTGTCATTGACGAGAATTCTGATCAGCCCCTTGCCGATACAAAAGATGCTTCTTTAAATAAAGAGAAATCTTCTTCTGAAATTGCTTATGAAGCGAAATCTTCTTCCGAAATTACTTGTGAAGTGCACGATGCAGGTCACACAGGAGATATTGGCACTGGTATGGAGCATG
CGCTTCCTCAAGCCCTATAAGTTCATTCTTTTCTTCAACACAGAAAACCAGTCAATACAGAAAATATGCCTTCACATAAAGATGAGTCCACTGTTGAAGATGCTTATGAAGCAGAAGATGCAAGTCATACAGGAGATATTGTCACAGCAAAGCACAAGGCCTCTAACTCGTCTCCAGAAGTTCATTGTAATCCCCAGCAC
AAAGAACCAGCGGATGCAGAAACATTGTCTTCACATGAAGATACAGGTCATACGAGAGACATTGATACAGCATTGGAGCATAGCGCTTCTCGGCGCTCGCTCATTCCCGGAAGTTCATTTTAATCCCAACCATCGAAAGTCGGTCCATAAATAAGATTTTATCTTCACAGTCAGTTAATCCCCAGTTGAAGGAGTCTGAA
ACTTCATATGGAGATAAATCTGTAATTGGGAGGGATGTCAATAGGGGTGCAGCTTTTTTAACTCATTTTGTGTAATTTAATTCTCCATGTTCCAGAGCTTTTTTTTCTTCTAAATTTTTATCACATATATTTCGGCGTGTTAGAAACGATACTAGTCTTCGGTTGAAGCTAAAATGATGCCACATTAACCACAAAGTTCC
AAGTTGTCTAGCAATAGTGTATTTTAGTGTATTTTTGATTGGGACCAATGGGTGAACATTGATGTGACTTAAAATGATTAAAATGAGATTTCAAATTTCAGAAAAAAAAA

Intron 2 GTCCTTTAAC ... GTATGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.22	97	1	31
A2	32.8	85	0	41.5
A3	29.66	83	1	35
A4	34.78	75	0	40
A5	24.39	31	0	10
A6	38.31	95	3.5	59
A7	34.65	256	7	135.8
A8	31.92	209	2.3	98
A9	32.29	261	1.5	124.5
A10	33.01	209	0.8	103

>TRINITY_DN43512_c0_g2_i3 from sample A6 / Example spliced isoform

CAGGTATATGGCTGATGGGCTTTATTTTTTCTGATTTCTTTTCATACACTCCGCATTTTTTTCCTTGTACCGTGTCCATCCTGTCCATCTCCAATTAATTTTAGAGACATCAATGGAGCATCGGGCTTCATTCCGAATCTTGTTTCAGACACTTTTCTATTAATGGAAATATCCCCACTTTCTACCCACAGGTTTCGAAA
GGTGTATCATCATTTGTTGAACCGATAGCTTAGTGTCTCTGAACAGCACTTGCACAGTCAATTCGAGTGACAAACATGGAGCCACCAGGAAGTAAAGATGTTTTATTTGATTCTGATTTTGAGTCGATGGAGCAAAGTAAGTCAGATAGAAAAGTCCAGGACTCTCTGTTGAAGTTTGTTGCAGATGCAGTTGGAAACAA
GCTTCTTATTGCATTTTCAAGATTTGGAAAGCATCCCGTGGAGTGGGGTCACTGGCTCGATGTTTTAGATGACGAGAATCTGAAAAGATTCTGCAAAGATGTGAATGAAGAAGAAATTCTTACAGATGAGAGTGTACAGGAAACAAATACAGCTGTTGATCATTCCTCTTCAGATTCTGATATTGAGGGCCCTAGTCCAA
TTGATGGCAGCCACTCTCTTATGATTGCACAAGGTGTAGCTGCGTATGCATCTGCTGCTGCAAAGTATAATTGTCTTGCTGAATGGCCTTTGCTGGGACCCTTTGACATACAGATGCTGACATGTGAGAAATGCTCTCAGCATTTCTTCTCTTCATTTAACTACCGAAGGCACACACAAACACATCGTCATGTTGTGAAG
AGTAAAAAGGAGAACTTAGTGAATGAGAGTGCTGACTTGAGGGGATACTGGGATAAGCTCCAGCCAGACAAGGCCAGAGACATAGTTTATTTTGGCAACATAATGTTTGAGGGGATTCCCGGAGACTCAGTTATAAAGTATTTGACATCTGTTGTTTGCCATTCCTGCATTCTTCCCCTGCCAAAAAGTGTTTTCAAGGC
CGGGAACTCCCTTCTGGAATTGTTACAAACCAAGTCCTCCAAGTTTCCATGCACATCAGAAGAACTATTTCACACACTGAATCTTGCAAGTGAGAGGACATTTTTGCAAACCAGAGCATCTGGTTCCTTGCAGAAATTTATTTTTGAGGGAGAAGAAGGGAAGTTAGGACACAAAATCAAGAATCTGGTTGCTTCCATAG
GCTTCCTGGTTGAGCAGAGACTGGTTAAGGCATGGATTACTGAGAAAGATGTAGAGTCCCTTCGCTTGCAAAAGAAGCTTATAGATGAAGAGGAAGCAGTGCAGAGAAGGAAGGCAGAGGAACGTGAGAGAAAGCTTTTGAATCAAGAGAAAAGAAAAGAACTGAATAAAAAGGATAGCACAGATGTGAGCAGTGAGGAT
TCCAACAAGTTCCTTGAAGTTGGGCTGAAAGAATTGAAAGATATTACGGCTGTCATTGACGAGAATTCTGATCAGCCCCTTGCCGATACAAAAGATGCTTCTTTAAATAAAGAGAAATCTTCTTCTGAAATTGCTTATGAAGCGAAATCTTCTTCCGAAATTACTTGTGAAGTGCACGATGCAGGTCACACAGGAGATAT
TGGCACTGGTATGGAGCATGCGCTTCCTCAAGCCCTATAAGTTCATTCTTTTCTTCAACACAGAAAACCAGTCAATACAGAAAATATGCCTTCACATAAAGATGAGTCCACTGTTGAAGATGCTTATGAAGCAGAAGATGCAAGTCATACAGGAGATATTGTCACAGCAAAGCACAAGGCCTCTAACTCGTCTCCAGAAG
TTCATTGTAATCCCCAGCACAAAGAACCAGCGGATGCAGAAACATTGTCTTCACATGAAGATACAGGTCATACGAGAGACATTGATACAGCATTGGAGCATAGCGCTTCTCGGCGCTCGCTCATTCCCGGAAGTTCATTTTAATCCCAACCATCGAAAGTCGGTCCATAAATAAGATTTTATCTTCACAGTCAGTTAATC
CCCAGTTGAAGGAGTCTGAAACTTCATATGGAGATAAATCTGTAATTGGGAGGGATGTCAATAGGGGTGCAGCTTTTTTAACTCATTTTGTGTAATTTAATTCTCCATGTTCCAGAGCTTTTTTTTCTTCTAAATTTTTATCACATATATTTCGGCGTGTTAGAAACGATACTAGTCTTCGGTTGAAGCTAAAATGATGC
CACATTAACCACAAAGTTCCAAGTTGTCTAGCAATAGTGTATTTTAGTGTATTTTTGATTGGGACCAATGGGTGAACATTGATGTGACTTAAAATGATTAAAATGAGG

>TRINITY_DN43512_c0_g2_i2 from sample A6 / Example unspliced isoform:

AATTTTCATGTTGTTCAACATCAGCGTATGCAACTAGCTGTGCATATCATTTGTTTGGTTTTTTATAATTTTGAGGTTGATTGTGCACTTGCTTCTCTAATCTTCAACCTTTCACAGGAGAACTTAGTGAATGAGAGTGCTGACTTGAGGGGATACTGGGATAAGgtcctttaaccacttatcatttcatcatcatgtta
taatctctggaggaactattttctgcatgcttatacctttgatttatagtataacagtttcttgttaatttggtatgtgcagCTCCAGCCAGACAAGGCCAGAGACATAGTTTATTTTGGCAACATAATGTTTGAGGGGATTCCCGGAGACTCAGTTATAAAGTATTTGACATCTGTTGTTTGCCATTCCTGCATTCTTC
CCCTGCCAAAAAGTGTTTTCAAGGCCGGGAACTCCCTTCTGGAATTGTTACAAACCAAGTCCTCCAAGTTTCCATGCACATCAGAAGAACTATTTCACACACTGAATCTTGCAAGTGAGAGGACATTTTTGCAAACCAGAGCATCTGGTTCCTTGCAGAAATTTATTTTTGAGGGAGAAGAAGGGAAGTTAGGACACAAA
ATCAAGAATCTGGTTGCTTCCATAGGCTTCCTGGTTGAGCAGAGACTGGTTAAGGCATGGATTACTGAGAAAGATGTAGAGTCCCTTCGCTTGCAAAAGAAGCTTATAGATGAAGAGGAAGCAGTGCAGAGAAGGAAGGCAGAGGAACGTGAGAGAAAGCTTTTGAATCAAGAGAAAAGAAAAGAACTGAATAAAAAGGA
TAGCACAGATGTGAGCAGTGAGGATTCCAACAAGTTCCTTGAAGTTGGGCTGAAAGAATTGAAAGATATTACGGCTGTCATTGACGAGAATTCTGATCAGCCCCTTGCCGATACAAAAGATGCTTCTTTAAATAAAGAGAAATCTTCTTCTGAAATTGCTTATGAAGCGAAATCTTCTTCCGAAATTACTTGTGAAGTGC
ACGATGCAGGTCACACAGGAGATATTGGCACTGGTATGGAGCATGCGCTTCCTCAAGCCCTATAAGTTCATTCTTTTCTTCAACACAGAAAACCAGTCAATACAGAAAATATGCCTTCACATAAAGATGAGTCCACTGTTGAAGATGCTTATGAAGCAGAAGATGCAAGTCATACAGGAGATATTGTCACAGCAAAGCAC
AAGGCCTCTAACTCGTCTCCAGAAGTTCATTGTAATCCCCAGCACAAAGAACCAGCGGATGCAGAAACATTGTCTTCACATGAAGATACAGGTCATACGAGAGACATTGATACAGCATTGGAGCATAGCGCTTCTCGGCGCTCGCTCATTCCCGGAAGTTCATTTTAATCCCAACCATCGAAAGTCGGTCCATAAATAAG
ATTTTATCTTCACAGTCAGTTAATCCCCAGTTGAAGGAGTCTGAAACTTCATATGGAGATAAATCTGTAATTGGGAGGGATGTCAATAGGGGTGCAGCTTTTTTTAACTCATTTTGTGTAATTTAATTCTCCATGTTCCAGAGCTTTTTTTTCTTCTAAATTTTTATCACATATATTTCGGCGTGTTAGAAACGATACTA
GTCTTCGGTTGAAGCTAAAATGATGCCACATTAACCACAAAGTTCCAAGTTGTCTAGCAATAGTGTATTTTAGTGTATTTTTGATTGGGACCAATGGGTGAACATTGATGTGACTTAAAATGATTAAAATGAGG

Intron 3 GTATCCAGTT ... CATGTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.68	148	27.7	34
A2	18.44	146	15.3	33
A3	6.94	161	25.3	12
A4	17.16	111	27	23
A5	24.19	47	6.7	15
A6	15.02	215	26.7	38
A7	16.96	586	72.3	119.7
A8	17.17	418	52.3	86.7
A9	16.59	548	52.7	109
A10	19.85	463	50.7	114.7

>TRINITY_DN114152_c0_g1_i8 from sample A2 / Example spliced isoform

GGAAATATCCCCACTTTCTACCCACAGGTTTCGAAAGGTGTATCATCATTTGTTGAACCGATAGCTTAGTGTCTCTGAACAGCACTTGCACAGTCAATTCGAGTGACAAACATGGAGCCACCAGGAAGTAAAGATGTTTTATTTGATTCTGATTTTGAGTCGATGGAGCAAAGTAAGTCAGATAGAAAAGTCCAGGACTC
TCTGTTGAAGTTTGTTGCAGATGCAGTTGGAAACAAGCTTCTTATTGCATTTTCAAGATTTGGAAAGCATCCCGTGGAGTGGGGTCACTGGCTCGATGTTTTAGATGACGAGAATCTGAAAAGATTCTGCAAAGATGTGAATGAAGAAGAAATTCTTACAGATGAGAGTGTACAGGAAACAAATACAGCTGTTGATCATT
CCTCTTCAGATTCTGATATTGAGGGCCCTAGTCCAATTGATGGCAGCCACTCTCTTATGATTGCACAAGGTGTAGCTGCGTATGCATCTGCTGCTGCAAAGTATAATTGTCTTGCTGAATGGCCTTTGCTGGGACCCTTTGACATACAGATGCTGACATGTGAGAAATGCTCTCAGCATTTCTTCTCTTCATTTAACTAC
CGAAGGCACACACAAACACATCGTCATGTTGTGAAGAGTAAAAAGGAGAACTTAGTGAATGAGAGTGCTGACTTGAGGGGATACTGGGATAAGCTCCAGCCAGACAAGGCCAGAGACATAGTTTATTTTGGCAACATAATGTTTGAGGGGATTCCCGGAGACTCAGTTATAAAGTATTTGACATCTGTTGTTTGCCATTC
CTGCATTCTTCCCCTGCCAAAAAGTGTTTTCAAGGCCGGGAACTCCCTTCTGGAATTGTTACAAACCAAGTCCTCCAAGTTTCCATGCACATCAGAAGAACTATTTCACACACTGAATCTTGCAAGTGAGAGGACATTTTTGCAAACCAGAGCATCTGGTTCCTTGCAGAAATTTATTTTTGAGGGAGAAGAAGGGAAGT
TAGGACACAAAATCAAGAATCTGGTTGCTTCCATAGGCTTCCTGGTTGAGCAGAGACTGGTTAAGGCATGGATTACTGAGAAAGATGTAGAGTCCCTTCGCTTGCAAAAGAAGCTTATAGATGAAGAGGAAGCAGTGCAGAGAAGGAAGGCAGAGGAACGTGAGAGAAAGCTTTTGAATCAAGAGAAAAGAAAAGAACTG
AATAAAAAGGATAGCACAGATGTGAGCAGTGAGGATTCCAACAAGTTCCTTGAAGTTGGGCTGAAAGAATTGAAAGATATTACGGCTGTCATTGACGAGAATTCTGATCAGCCCCTTGCCGATACAAAAGATGCTTCTTTAAATAAAGAGAAATCTTCTTCTGAAATTGCTTATGAAGCGAAATCTTCTTCCGAAATTAC
TTGTGAAGTGCACGATGCAGGTCACACAGGAGATATTGGCACTGGTATGGAGCATGCGCTTCCTCAAGCCCTATAAGTTCATTCTTTTCTTCAACACAGAAAACCAGTCAATACAGAAAATATGCCTTCACATAAAGATGAGTCCACTGTTGAAGATGCTTATGAAGCAGAAGATGCAAGTCATACAGGAGATATTGTCA
CAGCAAAGCACAAGGCCTCTAACTCGTCTCCAGAAGTTCATTGTAATCCCCAGCACAAAGAACCAGCGGATGCAGAAACATTGTCTTCACATGAAGATACAGGTCATACGAGAGACATTGATACAGCATTGGAGCATAGCGCTTCTCGGCGCTCGCTCATTCCCGGAAGTTCATTTTAATCCCAACCATCGAAAGTCGGT
CCATAAATAAGATTTTATCTTCACAGTCAGTTAATCCCCAGTTGAAGGAGTCTGAAACTTCATATGGAGATAAATCTGTAATTGGGAGGGATGTCAATAGGGGTGCAGCTTTTTTAACTCATTTTGTGTAATTTAATTCTCCATGTTCCAGAGCTTTTTTTTCTTCTAAATTTTTATCACATATATTTCGGCGTGTTAGA
AACGATACTAGTCTTCGGTTGAAGCTAAAATGATGCCACATTAACCACAAAGTTCCAAGTTGTCTAGCAATAGTGTATTTTAGTGTATTTTTGATTGGGAC

>TRINITY_DN114152_c0_g1_i2 from sample A2 / Example unspliced isoform:

CAAGGCCGGGAACTCCCTTCTGgtatccagtttgcattttgctctcacttagcattgtatagtagtatcttttctattttgatgctgacgcatccatgtaacagGAATTGTTACAAACCAAGTCCTCCAAGTTTCCATGCACATCAGAAGAACTATTTCACACACTGAATCTTGCAAGTGAGAGGACATTTTTGCAAACC
AGAGCATCTGGTTCCTTGCAGAAATTTATTTTTGAGGGAGAAGAAGGGAAGTTAGGACACAAAATCAAGAATCTGGTTGCTTCCATAGGCTTCTTCGTTGAGCAGAGACTGGTTAAGGCATGGATTACTGAGAAAGATGTAGAGTCCCTTCGCTTGCAAAAGAAGCTTATAGATGAAGAGGAAGCAGTGCAGAGAAGGAA
GGCAGAGGAACGTGAGAGAAAGCTTTTGAATCAAGAGAAAAGAAAAGAACTGAATAAAAAGGATAGCACAGATGTGAGCAGTGAGGATTCCAACAAGTTCCTTGAAGTTGGGCTGAAAGAATTGAAAGATATTACGGCTGTCATTGACGAGAATTCTGATCAGCCCCTTGCCGATACAAAAGATGCTTCTTTAAATAAAG
AGAAATCTTCTTCTGAAATTGCTTATGAAGCGAAATCTTCTTCCGAAATTACTTGTGAAGTGCACGATGCAGGTCACACAGGAGATATTGGCACTGGTATGGAGCATGCGCTTCCTCAAGCCCTATAAGTTCATTCTTTTCTTCAACACAGAAAACCAGTCAATACAGAAAATATGCCTTCACATAAAGATGAGTCCACT
GTTGAAGATGCTTATGAAGCAGAAGATGCAAGTCATACAGGAGATATTGTCACAGCAAAGCACAAGGCCTCTAACTCGTCTCCAGAAGTTCATTGTAATCCCCAGCACAAAGAACCAGCGGATGCAGAAACATTGTCTTCACATGAAGATACAGGTCATACGAGAGACATTGATACAGCATTGGAGCATAGCGCTTCTCG
GCGCTCGCTCATTCCCGGAAGTTCATTTTAATCCCAACCATCGAAAGTCGGTCCATAAATAAGATTTTATCTTCACAGTCAGTTAATCCCCAGTTGAAGGAGTCTGAAACTTCATATGGAGATAAATCTGTAATTGGGAGGGATGTCAATAGGGGTGCAGCTTTTTTAACTCATTTTGTGTAATTTAATTCTCCATGTTC
CAGAGCTTTTTTTTCTTCTAAATTTTTATCACATATATTTCGGCGTGTTAGAAACGATACTAGTCTTCGGTTGAAGCTAAAATGATGCCACATTAACCACAAAGTTCCAAGTTGTCTAGCAATAGTGTATTTTAGTGTATTTTTGATTGGGAC

Intron 4 GTATGCAGCA ... CCTTTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.37	30	1	27
A2	42.42	19	3	14
A3	50	14	6	14
A4	46.67	8	1	7
A5	44.44	10	2	8
A6	48	13	0	12
A7	49.38	41	17	40
A8	44.93	38	25	31
A9	46.91	43	7	38
A10	46.25	43	3	37

>TRINITY_DN47197_c0_g1_i3 from sample A7 / Example spliced isoform

TTTTTTCTGATTTCTTTTCATGCACTCCGCATTTTTTTCCTTGTACCGTGTCCATCCTGTCCATCTCCAATTAATTTTAGAGACATCAATGGAGCATCGGGCTTCATTCCGAATCTTGTTTCAGACACTTTTCTATTAATGGAAATATCCCCACTTTCTACCCACAGGTTTCGAAAGGTGTATCATCATTTGTTGAAGTA
AGCTTCTGTATTAAGCCGATAGCTTAGTGTCTCTGAACAGCACTTGCACAGTCAATTCGAGTGACAAACATGGAGCCACCAGGAAGTAAAGATGTTTTATTTGATTCTGATTTTGAGTCGATGGAGCAAAGTAAGTCAGATAGAAAAGTCCAGGACTCTCTGTTGAAGTTTGTTGCAGATGCAGTTGGAAACAAGCTTCT
TATTGCATTTTCAAGATTTGGAAAGCATCCCGTGGAGTGGGGTCACTGGCTCGATGTTTTAGATGACGAGAATCTGAAAAGATTCTGCAAAGATGTGAATGAAGAAGAAATTCTTACAGATGAGAGTGTACAGGAAACAAATACAGCTGTTGATCATTCCTCTTCAGATTCTGATATTGAGGGCCCTAGTCCAATTGATG
GCAGCCACTCTCTTATGATTGCACAAGGTGTAGCTGCGTATGCATCTGCTGCTGCAAAGTATAATTGTCTTGCTGAATGGCCTTTGCTGGGACCCTTTGACATACAGATGCTGACATGTGAGAAATGCTCTCAGCATTTCTTCTCTTCATTTAACTACCGAAGGCACACACAAACACATCGTCATGTTGTGAAGAGTAAA
AAGGTTGCCAACATAAAATTTTCTCAACAGTTTTAGAGAACTTAGTGAATGAGAGTGCTGACTTGAGGGGATACTGGGATAAGCTCCAGCCAGACAAGGCCAGAGACATAGTTTATTTTGGCAACATAATGTTTGAGGGGATTCCCGGAGACTCAGTTATAAAGTATTTGACATCTGTTGTTTGCCATTCCTGCATTCTT
CCCCTGCCAAAAAGTGTTTTCAAGGCCGGGAACTCCCTTCTGGAATTGTTACAAACCAAGTCCTCCAAGTTTCCATGCACATCAGAAGAACTATTTCACACACTGAATCTTGCAAGTGAGAGGACATTTTTGCAAACCAGAGCATCTGGTTCCTTGCAGAAATTTATTTTTGAGGGAGAAGAAGGGAAGTTAGGACACAA
AATCAAGAATCTGGTTGCTTCCATAGGCTTCCTGGTTGAGCAGAGACTGGTTAAGGCATGGATTACTGAGAAAGATGTAGAGTCCCTTCGCTTGCAAAAGAAGCTTATAGATGAAGAGGAAGCAGTGCAGAGAAGGAAGGCAGAGGAACGTGAGAGAAAGCTTTTGAATCAAGAGAAAAGAAAAGAACTGAATAAAAAGG
ATAGCACAGATGTGAGCAGTGAGGATTCCAACAAGTTCCTTGAAGTTGGGCTGAAAGAATTGAAAGATATTACGGCTGTCATTGACGAGAATTCTGATCAGCCCCTTGCCGATACAAAAGATGCTTCTTTAAATAAAGAGAAATCTTCTTCTGAAATTGCTTATGAAGCGAAATCTTCTTCCGAAATTACTTGTGAAGTG
CACGATGCAGGTCACACAGGAGATATTGGCACTGGTATGGAGCATGCGCTTCCTCAAGCCCTATAAGTTCATTCTTTTCTTCAACACAGAAAACCAGTCAATACAGAAAATATGCCTTCACATAAAGATGAGTCCACTGTTGAAGATGCTTATGAAGCAGAAGATGCAAGTCATACAGGAGATATTGTCACAGCAAAGCA
CAAGGCCTCTAACTCGTCTCCAGAAGTTCATTGTAATCCCCAGCACAAAGAACCAGCGGATGCAGAAACATTGTCTTCACATGAAGATACAGGTCATACGAGAGACATTGATACAGCATTGGAGCATAGCGCTTCTCGGCGCTCGCTCATTCCCAGAAGTTCATTCTAATCCCAACCATCGAAAGTCGGTCCATAAATAA
GATTTTATCTTCACAGTCAGTTAATCCCCAGTTGAAGGAGTCTGAAACTTCATATGGAGATAAATCTGTAATTGGGAGGGATGTCAATAGGGGTGCAGCTTTTTTAACTTATTTTGTGTAATTTAATGGCCCATGTTTCAGAGCTCTTTTTTCTTCTAAATTTTTATCACATATATTTCTGCGTCTTAGAAACGATACTA
GTCTTCCGGTGAAGCTAAAATGATGCCATATTAGCCACAAAGTTCCAAATTGTCTGGCAATAGTGTGTTTTAGTGTATTTCTGATTGGGACCAATGGGTGAACATTGATGTGACTTAAAATGATTAAAATGAGATTTCAAATTTCAGAAAAAAAAA

>TRINITY_DN47197_c0_g1_i7 from sample A7 / Example unspliced isoform:

CAGTAGATCAGTTATTTTCAAAAGAAGCTCAAATCGTTCTCAAAAACTTGTTTCATATACAGTAGATCAGTTATTTTCAAAAGAAGCTCAAATCGTTCTCAAAAACTTGTTTCATATATATATATAATGAAAGTCAACTTAAACGGATTCTTTTCCACTAACATGACAAAGTTTCTCCATTAAATTCTTTTATTTTGTTA
AACTAATGTTGATGAAATTTATGAAATAACATCTAAATGAGCACTTTTACCCTTGCAATAGAAAACGAGAAGGATCTAAATAATTCATCAGTGCAAAATGGTATCTAAACTATAGTGATTTTACTTGAGTCAGCAATCCCCAGATTCAAGCATCTAACTTCTTTCAGGTTTTTTAATTTTCCCGGAAGCTACAGTCATGC
TTTATGCTGGAGTGAAAACAAGACATCAAACTTATTGAATCTCTTAAAAGGATGAAGCAAGCTATTGCATATCAGATTTTGGTGGCCATGGTCATATTAGAGTTTTCAGACTAAATTTGCTAATTCAACATTAGATTGAATATTAATTAATATGTGTTTCTGGAAATGTAATTTTCTATATATTTTGTGCATAAAACAAC
AGATTTAATTGTATTTTTTATTGTATTAGAAAATCCAAAATTGATATTTGAATACTTCCCTACATACGAATACTATTTTGAAATGGTAGAAACTTGAAGCCATCCTTGATTATGTATCCTGTTATGTGTAGCCGATAGCTTAGTGTCTCTGAACAGCACTTGCACAGTCAATTCGAGTGACAAACATGGAGCCACCAGGA
AGTAAAGATGTTTTATTTGATTCTGATTTTGAGTCGATGGAGCAAAGTAAGTCAGATAGAAAAGTCCAGGACTCTCTGTTGAAGTTTGTTGCAGATGCAGTTGGAAACAAGCTTCTTATTGCATTTTCAAGATTTGGAAAGCATCCCGTGGAGTGGGGTCACTGGCTCGATGTTTTAGATGACGAGAATCTGAAAAGATT
CTGCAAAGATGTGAATGAAGAAGAAATTCTTACAGATGAGAGTGTACAGGAAACAAATACAGCTGTTGATCATTCCTCTTCAGATTCTGATATTGAGGGCCCTAGTCCAATTGATGGCAGCCACTCTCTTATGATTGCACAAGGTGTAGCTGCGTATGCATCTGCTGCTGCAAAGTATAATTGTCTTGCTGAATGGCCTT
TGCTGGGACCCTTTGACATACAGATGCTGACATGTGAGAAATGCTCTCAGCATTTCTTCTCTTCATTTAACTACCGAAGGCACACACAAACACATCGTCATGTTGTGAAGAGTAAAAAGGTTGCCAACATAAAATTTTCTCAACAGTTTTAgtatgcagcatgatccctgatttcttttgattgactaccataaatcttt
aatttcaattttcatgttgttcaacatcagcgtatgcaactagctgtgcatatcatttgtttggttttttataattttgaggttgattgtgcacttgcttctctaatcttcaacctttcacagGAGAACTTAGTGAATGAGAGTGCTGACTTGAGGGGATACTGGGATAAGCTCCAGCCAGACAAGGCCAGAGACATAGT
TTATTTTGGCAACATAATGTTTGAGGGGATTCCCGGAGACTCAGTTATAAAGTATTTGACATCTGTTGTTTGCCATTCCTGCATTCTTCCCCTGCCAAAAAGTGTTTTCAAGGCCGGGAACTCCCTTCTGGAATTGTTACAAACCAAGTCCTCCAAGTTTCCATGCACATCAGAAGAACTATTTCACACACTGAATCTTG
CAAGTGAGAGGACATTTTTGCAAACCAGAGCATCTGGTTCCTTGCAGAAATTTATTTTTGAGGGAGAAGAAGGGAAGTTAGGACACAAAATCAAGAATCTGGTTGCTTCCATAGGCTTCCTGGTTGAGCAGAGACTGGTTAAGGCATGGATTACTGAGAAAGATGTAGAGTCCCTTCGCTTGCAAAAGAAGCTTATAGAT
GAAGAGGAAGCAGTGCAGAGAAGGAAGGCAGAGGAACGTGAGAGAAAGCTTTTGAATCAAGAGAAAAGAAAAGAACTGAATAAAAAGGATAGCACAGATGTGAGCAGTGAGGATTCCAACAAGTTCCTTGAAGTTGGGCTGAAAGAATTGAAAGATATTACGGCTGTCATTGACGAGAATTCTGATCAGCCCCTTGCCGA
TACAAAAGATGCTTCTTTAAATAAAGAGAAATCTTCTTCTGAAATTGCTTATGAAGCGAAATCTTCTTCCGAAATTACTTGTGAAGTGCACGATGCAGGTCACACAGGAGATATTGGCACTGGTATGGAGCATGCGCTTCCTCAAGCCCTATAAGTTCATTCTTTTCTTCAACACAGAAAACCAGTCAATACAGAAAATA
TGCCTTCACATAAAGATGAGTCCACTGTTGAAGATGCTTATGAAGCAGAAGATGCAAGTCATACAGGAGATATTGTCACAGCAAAGCACAAGGCCTCTAACTCGTCTCCAGAAGTTCATTGTAATCCCCAGCACAAAGAACCAGCGGATGCAGAAACATTGTCTTCACATGAAGATACAGGTCATACGAGAGACATTGAT
ACAGCATTGGAGCATAGCGCTTCTCGGCGCTCGCTCATTCCCGGAAGTTCATTTTAATCCCAACCATCGAAAGTCGGTCCATAAATAAGATTTTATCTTCACAGTCAGTTAATCCCCAGTTGAAGGAGTCTGAAACTTCATATGGAGATAAATCTGTAATTGGGAGGGATGTCAATAGGGGTGCAGCTTTTTTAACTCAT
TTTGTGTAATTTAATTCTCCATGTTCCAGAGCTTTTTTTTCTTCTAAATTTTTATCACATATATTTCGGCGTGTTAGAAACGATACTAGTCTTCGGTTGAAGCTAAAATGATGCCACATTAACCACAAAGTTCCAAGTTGTCTAGCAATAGTGTATTTTAGTGTATTTTTGATTGGGACCAATGGGTGAACATTGATGTG
ACTTAAAATGATTAAAATGAGATTTCAAATTTCAGAAAAAAAAA