sp|B7ZWR7|OFUT7_ARATH ----> O-fucosyltransferase 7

Intron 1 GTCCCTACAT ... TGCATATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	4	10	0
A2	-1	0	0	0
A3	50	2	4	0
A4	100	0	0	2
A5	100	0	5	0
A6	100	0	4	1
A7	73.33	2	7	4
A8	64.71	3	6	5
A9	69.23	2	6	3
A10	100	0	2	4

>TRINITY_DN43167_c0_g5_i1 from sample A9 / Example spliced isoform

GCAAAGCCTTCCTGCAGTGGCTCGGTCCCAAGATGGAGAGTCTCGATGCACATGATCCAACGAGAGATCAACATTATGAAAGATAACCTCATCATTCATCATCAACGTTGCACGATACAGATACCCGCATTATCAATGCTACATTAGTAATTGCAGAGCTGGGAAAAGGATCATTATGGTAGAACTCAAGGTGATTCGAG
CTGCAAAATTAGATTCTCGCCTTGAAAATAATAACTTACCCCTGGATATCCAGAAGTTGCGTTGACGAGTGCACTATCATGCTTTGCATTTCGCCCCACACATTGAAGCAATGGGAAAGCTAAATGCGCAAGCTACCTAGGACATTTGAACTTACAGGTTGAGAGGTGAGAATGCTCGTCTCAAATCCCTCATCCGTTTT
AAAAAAAGGATTCTACCAGTGCCAAATCTTGTTTATTTGCGGCAGCCCCTTAGGAAGGCAACTTGCAGCAAGTGGATTTTGACGGAAGTGGATTCATGTAACATTCCATTTTCAGCACAAGCTGCCAAATGGATTTTGACATTTTCAATATGTAAGACTATATGCTTTTCTTCTCTGCAAATTGATGGCTGAACATTATA
AGCATGTGTTTCTTTGTTTGCAA

>TRINITY_DN43167_c0_g5_i3 from sample A9 / Example unspliced isoform:

GCAAAGCCTTCCTGCAGTGGCTCGGTCCCAAGATGGAGAGTCTCGATGCACATGATCCAACGAGAGATCAACATTATGAAAGATAACCTCATCATTCATCATCAACGTTGCACGATACAGgtccctacatggtcgacacttacatgggactccaatcgttgtctcactagtcaatggataagcactgcctcaccttcgcc
ataatcagaaaattcatggtgttcagttctacaagcttggaatttggtttgagggagggttttttacctagagattcgaccacagcatgaagtatgtttctttttggatatttggcctaagcttggagttttattcgagggttttgtctctgatgtagcattctggagtagtttatcacatttttcctgtaatggagctg
cgtgggagctgatttgccatttgttagagaagagtgatgttcaaaactttatatctgttagaagacagagagggcatggccccctaaatacccaaactcacaaggagatttagaggaataaagtctctgtaattagtagattgattgtttagtcgattcaaaggattggttgtcaagggtttcgattatttgggtatcag
ggcctgcatataagATACCCGCATTATCAATGCTACATTAGTAATTGCAGAGCTGGGAAAAGGATCATTATGGTAGAACTCAAGCAATTTTTTTGATGTTTTTGATGAGGAGTATTTTATAAATACTTTGGCGAATGACGTCGAAATTATGAAGCAACTTCCGAAGGAGTTTGCATATTTAACCAGAGGAGTAAAACATT
TCTGATGTTGGTGTGGAGTACTATCGGGAGGAGATAGCTCCTTTGTGGTAGAAGTATAATGTGATTCGAGCTGCAAAATTAGATTCTCGCCTTGAAAATAATAACTTACCCCTGGATATCCAGAAGTTGCGTTGACGAGTGCACTATCATGCTTTGCATTTCGCCCCACACATTGAAGCAATGGGAAAGCTAAATGCGCA
AGCTACCTAGGACATTTGAACTTACAGGTTGAGAGGTGAGAATGCTCGTCTCAAATCCCTCATCCGTTTTAAAAAAAGGATTCTACCAGTGCCAAATCTTGTTTATTTGCGGCAGCCCCTTAGGAAGGCAACTTGCAGCAAGTGGATTTTGACGGAAGTGGATTCATGTAACATTCCATTTTCAGCACAAGCTGCCAAAT
GGATTTTGACATTTTCAATATGTAAGACTATATGCTTTTCTTCTCTGCAAATTGATGGCTGAACATTATAAGCATGTGTTTCTTTGTTTGCAA

Intron 2 GTGAGGATAC ... AATGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.62	11	1	10
A2	28.57	5	3	2
A3	23.81	16	0	5
A4	0	5	0	0
A5	85.71	1	1	6
A6	57.14	6	8	8
A7	60	2	1	3
A8	45	11	2	9
A9	14.29	6	0	1
A10	36.36	14	0	8

>TRINITY_DN44698_c1_g1_i1 from sample A8 / Example spliced isoform

ATGAACCGTATGCAAAGCCTTCCTGCAGTGGCTCGGTCCCAAGATGGAGAGTCTCGATGCACATGATCCAACGAGAGATCAACATTATGAAAGATAACCTCATCATTCATCATCAACGTTGCACGATACAGGTCCCTACATGGTCGACACTTACATGGGACTCCAATCGTTGTCTCACTAGTCAATGGATAAGCACTGCC
TCACCTTCGCCATAATCAGAAAATTCATGGTGTTCAGTTCTACAAGCTTGGAATTTGGTTTGAGGGAGGGTTTTTTACCTAGAGATTCGACCACAGCATGAAATACCCGCATTATCAATGCTACATTAGTAATTGCAGAGCTGGGAAAAGGATCATTATGGTAGAACTCAAGCAATTTTTTTGATGTTTTTGATGAGGAG
TATTTTATAAATACTTTGGCGAATGACGTCGAAATTATGAAGCAACTTCCGAAGGAGTTTGCATATTTAACCAGAGGAGTAAAACATTTCTGATGTTGGTGTGGAGTACTATCGGGAGGAGATAGCTCCTTTGTGGTAGAAGTATAATGTGATTCGAGCTGCAAAATTAGATTCTCGCCTTGAAAATAATAACTTACCCC
TGGATATCCAGAAGTTGCGTTGACGAGTGCACTATCATGCTTTGCATTTCGCCCCACACATTGAAGCAATGGGAAAGCTAAATGCGCAAGCTACCTAGGACATTTGAACTTACAGGTTGAGAGGTGAGAATGCTCGTCTCAAATCCCTCATCCGTTTTAAAAAAAGGATTCTACCAGTGCCAAATCTTGTTTATTTGCGG
CAGCCCCTTAGGAAGGCAACTTGCAGCAAGTGGATTTTGACGGAAGTGGATTCATGTAACATTCCATTTTCAGCACAAGCTGCCAAATGGATTTTGACATTTTCAATATGTAAGACTATATGCTTTTCTTCTCTGCAAATTGATGGCTGAACATTATAAGCATGTGTTTCTTTGTTT

>TRINITY_DN44698_c1_g1_i2 from sample A8 / Example unspliced isoform:

GGAGTTTTATTCGAGGGTTTTGTCTCTGATGTAGCATTCTGGAGTAGTTTATCACATTTTTCCTGTAATGGAGCTGCGTGGGAGCTGATTTGCCATTTGTTAGAGAAGAGTGATGTTCAAAACTTTATATCTGTTAGAAGACAGAGAGGGCATGGCCCCCTAAATACCCAAACTCACAAGGAGATTTAGAGGAATAAAGT
CTCTGTAATTAGTAGATTGATTGTTTAGTCGATTCAAAGGATTGGTTGTCAAGGGTTTCGATTATTTGGGTATCAGGGCCTGCATATAAGATACCCGCATTATCAATGCTACATTAGTAATTGCAGAGCTGGGAAAAGGATCATTATGGTAGAACTCAAGgtgaggatactgtttttatctctgtaaaatattttaatat
aattcgtgaaaggtagctctatggtgtgcctctgcaagttccttttggtcttgcaatttggaatgttggaaaattgaaatgttgcagCAATTTTTTTGATGTTTTTGATGAGGAGTATTTTATAAATACTTTGGCGAATGACGTCGAAATTATGAAGCAACTTCCGAAGGAGTTTGCATATTTAACCAGAGGAGTAAAAC
ATTTCTGATGTTGGTGTGGAGTACTATCGGGAGGAGATAGCTCCTTTGTGGTAGAAGTATAATGTGATTCGAGCTGCAAAATTAGATTCTCGCCTTGAAAATAATAACTTACCCCTGGATATCCAGAAGTTGCGTTGACGAGTGCACTATCATGCTTTGCATTTCGCCCCACACATTGAAGCAATGGGAAAGCTAAATGC
GCAAGCTACCTAGGACATTTGAACTTACAGGTTGAGAGGTGAGAATGCTCGTCTCAAATCCCTCATCCGTTTTAAAAAAAGGATTCTACCAGTGCCAAATCTTGTTTATTTGCGGCAGCCCCTTAGGAAGGCAACTTGCAGCAAGTGGATTTTGACGGAAGTGGATTCATGTAACATTCCATTTTCAGCACAAGCTGCCA
AATGGATTTTGACATTTTCAATATGTAAGACTATATGCTTTTCTTCTCTGCAAATTGATGGCTGAACATTATAAGCATGTGTTTCTTTGTTT

Intron 3 GTATGTTTCT ... TGCATATAAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	4	0
A2	55.56	2	5	0
A3	100	0	2	0
A4	33.33	2	0	2
A5	66.67	1	4	0
A6	100	0	2	1
A7	85.71	1	8	4
A8	50	3	1	5
A9	53.85	3	4	3
A10	44.44	5	4	4

>TRINITY_DN43167_c0_g5_i2 from sample A9 / Example spliced isoform

GCAAAGCCTTCCTGCAGTGGCTCGGTCCCAAGATGGAGAGTCTCGATGCACATGATCCAACGAGAGATCAACATTATGAAAGATAACCTCATCATTCATCATCAACGTTGCACGATACAGGTCCCTACATGGTCGACACTTACATGGGACTCCAATCGTTGTCTCACTAGTCAATGGATAAGCACTGCCTCACCTTCGCC
ATAATCAGAAAATTCATGGTGTTCAGTTCTACAAGCTTGGAATTTGGTTTGAGGGAGGGTTTTTTACCTAGAGATTCGACCACAGCATGAAATACCCGCATTATCAATGCTACATTAGTAATTGCAGAGCTGGGAAAAGGATCATTATGGTAGAACTCAAGCAATTTTTTTGATGTTTTTGATGAGGAGTATTTTATAAA
TACTTTGGCGAATGACGTCGAAATTATGAAGCAACTTCCGAAGGAGTTTGCATATTTAACCAGAGGAGTAAAACATTTCTGATGTTGGTGTGGAGTACTATCGGGAGGAGATAGCTCCTTTGTGGTAGAAGTATAATGTGATTCGAGCTGCAAAATTAGATTCTCGCCTTGAAAATAATAACTTACCCCTGGATATCCAG
AAGTTGCGTTGACGAGTGCACTATCATGCTTTGCATTTCGCCCCACACATTGAAGCAATGGGAAAGCTAAATGCGCAAGCTACCTAGGACATTTGAACTTACAGGTTGAGAGGTGAGAATGCTCGTCTCAAATCCCTCATCCGTTTTAAAAAAAGGATTCTACCAGTGCCAAATCTTGTTTATTTGCGGCAGCCCCTTAG
GAAGGCAACTTGCAGCAAGTGGATTTTGACGGAAGTGGATTCATGTAACATTCCATTTTCAGCACAAGCTGCCAAATGGATTTTGACATTTTCAATATGTAAGACTATATGCTTTTCTTCTCTGCAAATTGATGGCTGAACATTATAAGCATGTGTTTCTTTGTTTGCAA

>TRINITY_DN43167_c0_g5_i3 from sample A9 / Example unspliced isoform:

GCAAAGCCTTCCTGCAGTGGCTCGGTCCCAAGATGGAGAGTCTCGATGCACATGATCCAACGAGAGATCAACATTATGAAAGATAACCTCATCATTCATCATCAACGTTGCACGATACAGGTCCCTACATGGTCGACACTTACATGGGACTCCAATCGTTGTCTCACTAGTCAATGGATAAGCACTGCCTCACCTTCGCC
ATAATCAGAAAATTCATGGTGTTCAGTTCTACAAGCTTGGAATTTGGTTTGAGGGAGGGTTTTTTACCTAGAGATTCGACCACAGCATGAAgtatgtttctttttggatatttggcctaagcttggagttttattcgagggttttgtctctgatgtagcattctggagtagtttatcacatttttcctgtaatggagctg
cgtgggagctgatttgccatttgttagagaagagtgatgttcaaaactttatatctgttagaagacagagagggcatggccccctaaatacccaaactcacaaggagatttagaggaataaagtctctgtaattagtagattgattgtttagtcgattcaaaggattggttgtcaagggtttcgattatttgggtatcag
ggcctgcatataagATACCCGCATTATCAATGCTACATTAGTAATTGCAGAGCTGGGAAAAGGATCATTATGGTAGAACTCAAGCAATTTTTTTGATGTTTTTGATGAGGAGTATTTTATAAATACTTTGGCGAATGACGTCGAAATTATGAAGCAACTTCCGAAGGAGTTTGCATATTTAACCAGAGGAGTAAAACATT
TCTGATGTTGGTGTGGAGTACTATCGGGAGGAGATAGCTCCTTTGTGGTAGAAGTATAATGTGATTCGAGCTGCAAAATTAGATTCTCGCCTTGAAAATAATAACTTACCCCTGGATATCCAGAAGTTGCGTTGACGAGTGCACTATCATGCTTTGCATTTCGCCCCACACATTGAAGCAATGGGAAAGCTAAATGCGCA
AGCTACCTAGGACATTTGAACTTACAGGTTGAGAGGTGAGAATGCTCGTCTCAAATCCCTCATCCGTTTTAAAAAAAGGATTCTACCAGTGCCAAATCTTGTTTATTTGCGGCAGCCCCTTAGGAAGGCAACTTGCAGCAAGTGGATTTTGACGGAAGTGGATTCATGTAACATTCCATTTTCAGCACAAGCTGCCAAAT
GGATTTTGACATTTTCAATATGTAAGACTATATGCTTTTCTTCTCTGCAAATTGATGGCTGAACATTATAAGCATGTGTTTCTTTGTTTGCAA