sp|Q8GXL3|SPL8_ARATH ----> Squamosa promoter-binding-like protein 8

Intron 1 GTAAGCATTT ... TTCATCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.45	6	5	3
A2	43.48	13	10	1
A3	47.37	10	9	5
A4	42.86	4	3	0
A5	42.86	4	3	0
A6	41.79	39	28	4
A7	39.53	26	17	4
A8	43.75	18	14	2
A9	41.46	24	17	3
A10	40	15	10	4

>TRINITY_DN31292_c0_g1_i3 from sample A6 / Example spliced isoform

GATGAACAACAATAGTAATAATAATAGTAATACTACTACTACTAACAATCCTATGCTGCAGCAACCAAATTCTCAGAAGGTTCCTGTAGCGAACCAGCAATACGTGCAGCAGCAGAGCGGAGGACACAAGGGCCCGAATTTATCTCTGTCGTCGTCCTCTGTGGGAATGAGTTTGTGCAATCAGAGGCAAACAACGGCAC
AGGATTATAGCGGGCTGGAGCTGGCGCTGCCATGGCTCAGAACAACATCAGAATACAGCGGATCGACCCATAGACCTCCTCCAGCTATGGACAATATCCTTCATCCTATTATATCTCATGAGAAGGCAAATGTTGAAGCTTTCTCGGCCTCTTCGGTTCAGAACTTTCTGCCTCTAACAGGCTCAATGAGTTCCAGAGAG
CTCGGTAGCTCTCAATGGATGGTTGGGAGCAGTGATAGCGCGAGCAGCCTGGAAGCTGGCCAGCAGATGTTCAGCTTGTTGGAGCCAGCTGAACATCAGAATAGAACGGAGGAACAAGCGCAGACGGCTCGGCCAGTAGAATATTTGTCTCATCGTCATCAAGGGGGTCTGAATTCCAAGGAGCCAGTAGATGGAAATTC
CGAAGCAAAATTCTCCGATATGCAAAATTCCCTGAGACCATTCGGCCAGTCTATTTATGACGGAAGTATGTGACCCGAATAAGAGACGTCAGATCTCTAATATTGATTGGATTTACAGATGAACCGGTGGAGTTATTGGGATTAGTTAGCCCTAAAAGCATGCCACAAGTTTCAGGAACTATTGCTGTTAAAAGTGCTTT
CTTAATGCTATTCAGGCCACATGTGAAGGGTGCATAGCTTAATAGAGTTCCGGAGCCTGCCCATGATCATTATTAGGGCAATTCCTCTTTTACTTAACTCTACATTACATTTCAGCATTTTCTGTGGAGTGAAATCCTGTAACAGCAGTCCGATCCTCTACAATGAAGCTCCAGGTCTTTTACAGGTCTTTTCGTTCTTG
TCCATGAATAAGCAAACACAGCTTTTTCTCATGAATAATATGCTTCATTAATTTGTTCTTCCATTGTCTTTTCGTTCTGC

>TRINITY_DN31292_c0_g1_i2 from sample A6 / Example unspliced isoform:

GATGAACAACAATAGTAATAATAATAGTAATACTACTACTACTAACAATCCTATGCTGCAGCAACCAAATTCTCAGAAGGTTCCTGTAGCGAACCAGCAATACGTGCAGCAGCAGAGCGGAGGACACAAGGGCCCGAATTTATCTCTGTCGTCGTCCTCTGTGGGAATGAGTTTGTGCAATCAGAGGCAAACAACGGCAC
AGGATTATAGCGGGCTGGAGCTGGCGCTGCCATGGCTCAGAACAACATCAGAATACAGCGGATCGACCCATAGACCTCCTCCAGCTATGGACAATATCCTTCATCCTATTATATCTCATGAGAAGGCAAATGTTGAAGCTTTCTCGGCCTCTTCGGTTCAGAACTTTCTGCCTCTAACAGGCTCAATGAGTTCCAGAGAG
CTCGGTAGCTCTCAATGGATGGTTGGGAGCAGTGATAGCGCGAGCAGCCTGGAAGCTGGCCAGCAGATGTTCAGCTTGTTGGAGCCAGCTGAACATCAGAATAGAACGGAGGAACAAGCGCAGACGGCTCGGCCAGTAGAATATTTGTCTCATCGTCATCAAGGGGGTCTGAATTCCAAGGAGCCAGTAGATGGAAATTC
CGAAGCAAAATTCTCCGATATGCAAAATTCCCTGAGACCATTCGGCCAGTCTATTTATGACGGAAGTATGTGACCCGAATAAGAGACGTCAGATCTCTAATATTgtaagcattttcagactccagggattcaaactcattgaaatgtagtgtagcagaatagctaattgtttaagttagtgattcaatgcatgtacgtgt
tctgttagtaatccaatctaatgtaacgaattattcatcacagGATTGGATTTACAGATGAACCGGTGGAGTTATTGGGATTAGTTAGCCCTAAAAGCATGCCACAAGTTTCAGGAACTATTGCTGTTAAAAGTGCTTTCTTAATGCTATTCAGGCCACATGTGAAGGGTGCATAGCTTAATAGAGTTCCGGAGCCTGCC
CATGATCATTATTAGGGCAATTCCTCTTTTACTTAACTCTACATTACATTTCAGCATTTTCTGTGGAGTGAAATCCTGTAACAGCAGTCCGATCCTCTACAATGAAGCTCCAGGTCTTTTACAGGTCTTTTCGTTCTTGTCCATGAATAAGCAAACACAGCTTTTTCTCATGAATAATATGCTTCATTAATTTGTTCTTC
CATTGTCTTTTCGTTCTGC

Intron 2 GTAAGCATTT ... GGATTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	81.82	1	5	4
A2	58.62	6	10	7
A3	65.22	4	9	6
A4	100	0	3	0
A5	100	0	3	0
A6	74.47	6	28	7
A7	68.75	5	17	5
A8	89.47	1	14	3
A9	72.41	4	17	4
A10	63.64	4	10	4

>TRINITY_DN31292_c0_g1_i1 from sample A6 / Example spliced isoform

AGAGACGTCAGATCTCTAATATTATGAACCGGTGGAGTTATTGGGATTAGTTAGCCCTAAAAGCATGCCACAAGTTTCAGGAACTATTGCTGTTAAAAGTGCTTTCTTAATGCTATTCAGGCCACATGTGAAGGGTGCATAGCTTAATAGAGTTCCGGAGCCTGCCCATGATCATTATTAGGGCAATTCCTCTTTTACTT
AACTCTACATTACATTTCAGCATTTTCTGTGGAGTGAAATCCTGTAACAGCAGTCCGATCCTCTACAATGAAGCTCCAGGTCTTTTACAGGTCTTTTCGTTCTTGTCCATGAATAAGCAAACACAGCTTTTTCTCATGAATAATATGCTTCATTAATTTGTTCTTCCATTGTCTTTTCGTTCTGC

>TRINITY_DN31292_c0_g1_i2 from sample A6 / Example unspliced isoform:

GATGAACAACAATAGTAATAATAATAGTAATACTACTACTACTAACAATCCTATGCTGCAGCAACCAAATTCTCAGAAGGTTCCTGTAGCGAACCAGCAATACGTGCAGCAGCAGAGCGGAGGACACAAGGGCCCGAATTTATCTCTGTCGTCGTCCTCTGTGGGAATGAGTTTGTGCAATCAGAGGCAAACAACGGCAC
AGGATTATAGCGGGCTGGAGCTGGCGCTGCCATGGCTCAGAACAACATCAGAATACAGCGGATCGACCCATAGACCTCCTCCAGCTATGGACAATATCCTTCATCCTATTATATCTCATGAGAAGGCAAATGTTGAAGCTTTCTCGGCCTCTTCGGTTCAGAACTTTCTGCCTCTAACAGGCTCAATGAGTTCCAGAGAG
CTCGGTAGCTCTCAATGGATGGTTGGGAGCAGTGATAGCGCGAGCAGCCTGGAAGCTGGCCAGCAGATGTTCAGCTTGTTGGAGCCAGCTGAACATCAGAATAGAACGGAGGAACAAGCGCAGACGGCTCGGCCAGTAGAATATTTGTCTCATCGTCATCAAGGGGGTCTGAATTCCAAGGAGCCAGTAGATGGAAATTC
CGAAGCAAAATTCTCCGATATGCAAAATTCCCTGAGACCATTCGGCCAGTCTATTTATGACGGAAGTATGTGACCCGAATAAGAGACGTCAGATCTCTAATATTgtaagcattttcagactccagggattcaaactcattgaaatgtagtgtagcagaatagctaattgtttaagttagtgattcaatgcatgtacgtgt
tctgttagtaatccaatctaatgtaacgaattattcatcacaggattggatttacagATGAACCGGTGGAGTTATTGGGATTAGTTAGCCCTAAAAGCATGCCACAAGTTTCAGGAACTATTGCTGTTAAAAGTGCTTTCTTAATGCTATTCAGGCCACATGTGAAGGGTGCATAGCTTAATAGAGTTCCGGAGCCTGCC
CATGATCATTATTAGGGCAATTCCTCTTTTACTTAACTCTACATTACATTTCAGCATTTTCTGTGGAGTGAAATCCTGTAACAGCAGTCCGATCCTCTACAATGAAGCTCCAGGTCTTTTACAGGTCTTTTCGTTCTTGTCCATGAATAAGCAAACACAGCTTTTTCTCATGAATAATATGCTTCATTAATTTGTTCTTC
CATTGTCTTTTCGTTCTGC