sp|Q8L7T9|MNM1_ARATH ----> Protein METABOLIC NETWORK MODULATOR 1

Intron 1 GTTTGATATT ... TGGAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.61	118	22	68
A2	26.76	78	12	45
A3	22.39	104	15	45
A4	34.39	62	20	45
A5	38.74	34	7	36
A6	23.6	395	50	194
A7	22.43	555	85	236
A8	23.22	362	53	166
A9	23.02	530	100	217
A10	24.31	425	73	200

>TRINITY_DN99642_c0_g1_i1 from sample A5 / Example spliced isoform

CGATTGAGCTGTTCATATCGAGATTTCATTTACTAGAAATCCGAGCAAGATCAAAACCTGGTGGTTTTAAAACGCGGTTTAAATCGCCCCAAATTCACATACGACGGCTGGTTTTAAAACTGCTGGTTTCGATTCGTGCGGCTTCTCGTTGGTTGTATAGCAGGCGACGCAAAATACCCGATCGAACAACACCGGCTTCG
CAGGGAAGCATTTTGACCCTGTTTCTCACTATTTACCGTCTCATTTTCCTTAACGGTCTTTGACATTTTGCCCTCGCGAGCATGCAGCCCCTCTAAATCACCCACAGGTGTTTAAAATGAAAGACGAAAATCAAGAAGCTCAGACTGATTCCACAGATATACCTCCAAAACGGAAGCGAGGGCGCCCTCGTAAATCACAC
GTTTCGTATCCAGGTGAGAAGGCTCATGTACCACAGCGGTTGGATGGGAAAAAGAAACCAAGACGCAAGAAAATAGATTCGTCAACGCCTTCATTTCCTGACAATACTTTTGTAGGCCAATCAGTTCATGGTGTATTGGATGGTTCATTTGATGCTGGCTACTTGCTGATGGTTAGAGTGGGAGACACTGATACAGTCCT
GCGAGGTGTTGTCTTCGATCCAGGAATGTCAGTGCCTGTTTCTAAAGCAAATGACATTGCACCGCATGTCAATCTGACAAGGAGAGATGAGAACATTCTTCTACCCCCTGAGCTTCCTCCATCTATTCACAATACTGTAGCTATGCCATCAACTCCTGTTCCTTTATTGCCAAATCTGGTGCCTGTTGTGAATAGTCAGG
GAACAACATCAGTTCCTATACCAGATCGATCTGCACCTCCAGTGCAGGTTGGCTCAAATCAAACAAGGGTTGTTTCGGGTCAACCAAATGGGAGCTTACCAAAGTCCGTACCTGATCATTCTAGTCAGATCAACAAAGAGCCTTTCCCAAAGCCAGGAGAATCTTTCTCTTACTCGCAGCAGTCGATCCCCATTCCCACC
GGACCAGTCTCTCGTCCACAGCAGCCAAACCCCATTCTCACCGGATCTGTCTCTCGTCTGCAGCAGCCAATCCCCAGTCTCAGTGAACATGTTTCACACACACAGCAACCAATCCCCACCCCTGCTGAACCTGTTTTTCGTCCAATGCAGTCAATTCCCAAACCTGCTGAACCCGTTTCCCATCCACAACATCCAACCCA
CCCATTTACTGAACCTGTCTCTCACCCACAGTAGCCAATTCCTCACTCTGGATATCCTGTCAAATCATAGATGAGTATTACTGATAAGCAAGCTTTTAAGGTACTGAATTTGTTCTGTTGACTGGTTATTAGAATGCTTGCCTCTAAATTTTTTGGTAAGCATCATTGTCAACGGCTAATCTATTGAGCATTAGATGTGC
CTGTTAAGTTTGTTCTTCTAAATGCTACCCAGAGAAGAATATTTTCTGCAAGTTTACTGACTATCTATTTCCAAGATTGGGCGATCTTCTATATCCATGTCTTATAAATGTTGTTATTTTGTTGTGCTCTAATTCCACAAAGTACTATTATTATCTCTATATTTCCC

>TRINITY_DN99642_c0_g1_i2 from sample A5 / Example unspliced isoform:

CGATTGAGCTGTTCATATCGAGATTTCATTTACTAGAAATCCGAGCAAGATCAAAACCTGGTGGTTTTAAAACGCGGTTTAAATCGCCCCAAATTCACATACGACGGCTGGTTTTAAAACTGCTGGTTTCGATTCGTGCGGCTTCTCGTTGGTTGTATAGCAGGCGACGCAAAATACCCGATCGAACAACACCGGCTTCG
CAGGGAAGCATTTTGACCCTGTTTCTCACTATTTACCGTCTCATTTTCCTTAACGGTCTTTGACATTTTGCCCTCGCGAGCATGCAGCCCCTCTAAATCACCCACAGgtttgatatttatttattattttcccctcttttgtctgtttcatcttcggtaagagatgtaaatctgtacatacgagaaatctttcttattgt
tttcttttctaaaaaccttatgttaccttactgtaatgtatttattttcttcaacggaaattatgttatttacattatttgaaacatcacagtacaaatttgccgcagccattaacagtgcgagacccctttcactgcggagacactgtagtgatagtttagcagactattcggacccgataaagagagggttttagagc
ttacaagctgggttttatggggttttaactatatttcgttggtcacttaatagccctagattgttttagggcaattttttggtcgttagtagaccattattaatatcattgtggttgtgtggcattttgacggctccctcgatttactacagtgatatagttggatatcataagggttgctgtgttgaagcgaggttgga
ttttcatatggagtgcagGTGTTTAAAATGAAAGACGAAAATCAAGAAGCTCAGACTGATTCCACAGATATACCTCCAAAACGGAAGCGAGGGCGCCCTCGTAAATCACACGTTTCGTATCCAGGTGAGAAGGCTCATGTACCACAGCGGTTGGATGGGAAAAAGAAACCAAGACGCAAGAAAATAGATTCGTCAACGCC
TTCATTTCCTGACAATACTTTTGTAGGCCAATCAGTTCATGGTGTATTGGATGGTTCATTTGATGCTGGCTACTTGCTGATGGTTAGAGTGGGAGACACTGATACAGTCCTGCGAGGTGTTGTCTTCGATCCAGGAATGTCAGTGCCTGTTTCTAAAGCAAATGACATTGCACCGCATGTCAATCTGACAAGGAGAGATG
AGAACATTCTTCTACCCCCTGAGCTTCCTCCATCTATTCACAATACTGTAGCTATGCCATCAACTCCTGTTCCTTTATTGCCAAATCTGGTGCCTGTTGTGAATAGTCAGGGAACAACATCAGTTCCTATACCAGATCGATCTGCACCTCCAGTGCAGGTTGGCTCAAATCAAACAAGGGTTGTTTCGGGTCAACCAAAT
GGGAGCTTACCAAAGTCCGTACCTGATCATTCTAGTCAGATCAACAAAGAGCCTTTCCCAAAGCCAGGAGAATCTTTCTCTTACTCGCAGCAGTCGATCCCCATTCCCACCGGACCAGTCTCTCGTCCACAGCAGCCAAACCCCATTCTCACCGGATCTGTCTCTCGTCTGCAGCAGCCAATCCCCAGTCTCAGTGAACA
TGTTTCACACACACAGCAACCAATCCCCACCCCTGCTGAACCTGTTTTTCGTCCAATGCAGTCAATTCCCAAACCTGCTGAACCCGTTTCCCATCCACAACATCCAACCCACCCATTTACTGAACCTGTCTCTCACCCACAGTAGCCAATTCCTCACTCTGGATATCCTGTCAAATCATAGATGAGTATTACTGATAAGC
AAGCTTTTAAGGTACTGAATTTGTTCTGTTGACTGGTTATTAGAATGCTTGCCTCTAAATTTTTTGGTAAGCATCATTGTCAACGGCTAATCTATTGAGCATTAGATGTGCCTGTTAAGTTTGTTCTTCTAAATGCTACCCAGAGAAGAATATTTTCTGCAAGTTTACTGACTATCTATTTCCAAGATTGGGCGATCTTC
TATATCCATGTCTTATAAATGTTGTTATTTTGTTGTGCTCTAATTCCACAAAGTACTATTATTATCTCTATATTTCCC

Intron 2 GTAGGCATCT ... TGTCCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	2	4	0
A2	66.67	1	2	0
A3	50	1	1	0
A4	100	0	3	0
A5	100	0	2	0
A6	88.24	2	15	1
A7	66.67	8	16	1
A8	77.78	12	42	2
A9	69.23	8	18	2
A10	92.86	2	26	0

>TRINITY_DN35203_c0_g1_i4 from sample A8 / Example spliced isoform

TTAGAAGTTCCACTTTGACGCTCGATCATTTTTATTTCAATGCCCTCCACCGCCGATTGAGCTGTTCATATCGAGATTTCATTTACTAGAAATCCGAGCAAGATCAAAACCTGGTGGTTTTAAAACGCGGTTTAAATCGCCCCAAATTCACATACGACGGCTGGTTTTAAAACTGCTGGTTTCGATTCGTGCGGCTTCTC
GTTGGTTGTATAGCAGGCGACGCAAAATACCCGATCGAACAACACCGGCTTCGCAGGGAAGCATTTTGACCCTGTTTCTCACTATTTACCGTCTCATTTTCCTTAACGGTCTTTGACATTTTGCCCTCGCGAGCATGCAGCCCCTCTAAATCACCCACAGTGCAGGTGTTTAAAATGAAAGACGAAAATCAAGAAGCTCA
GACTGATTCCACAGATATACCTCCAAAACGGAAGCGAGGGCGCCCTCGTAAATCACACGTTTCGTATCCAGGTGAGAAGGCTCATGTACCACAGCGGTTGGATGGGAAAAAGAAACCAAGACGCAAGAAAATAGATTCGTCAACGCCTTCATTTCCTGACAATACTTTTGTAGGCCAATCAGTTCATGGTGTATTGGATG
GTTCATTTGATGCTGGCTACTTGCTGATGGTTAGAGTGGGAGACACTGATACAGTCCTGCGAGGTGTTGTCTTCGATCCAGGAATGTCAGTGCCTGTTTCTAAAGCAAATGACATTGCACCGCATGTCAATCTGACAAGGAGAGATGAGAACATTCTTCTACCCCCTGAGCTTCCTCCATCTATTCACAATACTGTAGCT
ATGCCATCAACTCCTGTTCCTTTATTGCCAAATCTGGTGCCTGTTGTGAATAGTCAGGGAACAACATCAGTTCCTATACCAGATCGATCTGCACCTCCAGTGCAGGTTGGCTCAAATCAAACAAGGGTTGTTTCGGGTCAACCAAATGGGAGCTTACCAAAGTCCGTACCTGATCATTCTAGTCAGATCAACAAAGAGCC
TTTCCCAAAGCCAGGAGAATCTTTCTCTTACTCGCAGCAGTCGATCCCCATTCCCACCGGACCAGTCTCTCGTCCACAGCAGCCAAACCCCATTCTCACCGGATCTGTCTCTCGTCTGCAGCAGCCAATCCCCAGTCTCAGTGAACATGTTTCACACACACAGCAACCAATCCCCACCCCTGCTGAACCTGTTTTTCGTC
CAATGCAGTCAATTCCCAAACCTGCTGAACCCGTTTCCCATCCACAACATCCAACCCACCCATTTACTGAACCTGTCTCTCACCCACAGTAGCCAATTCCTCACTCTGGATATCCTGTCAAATCATAGATGAGTATTACTGATAAGCAAGCTTTTAAGCCACAATCACATGAAGGACACGAGACATTTTCCAGAATTGTT
TGTCACAAGCACCCATTCATAAATTACCAGGCATTCAGGGAGCAAACGAAAACACATAAGAAGGGCTGTGAATAGAATTGACTTTGTTTCACAGGAGTTGCTATCAAGATTGAATCATTTGGTCAATATATAACACTCTTCCTGGCAGATTGGCTACCCAGTTGCTTGATAACTATCTAGCTAATATTGAACCAGTGCTT
GATTCTAGGCAAACTGGCCACACGTTGAATGGATGCTTTTCCCTACTGAAAATCAGTTACAACTCGTCATCGAGGTT

>TRINITY_DN35203_c0_g1_i2 from sample A8 / Example unspliced isoform:

AAATCACACGTTTCGTATCCAGGGGTCTTGCAAGAAGGCTTGGTTATACATACAATTCATTCTTTGGAGGCTGTGCCCCATATCACTGGGATTTGAAGCTAACTCTTGTATTTGAAAGTGGATTTGGAAGAAAGAATGGTGATAATTAGCCACAATCACATGAAGGACACGAGACATTTTCCAGAATTGTTTGTCACAAG
CACCCATTCATAAATTACCAGGCATTCAGGGAGCAAACGAAAACACATAAGAAGGGCTGTGAATAGAATTGACTTTGTTTCACAGGAGTTGCTATCAAGATTGAATCATTTGGTCAATATATAACACTCTTCCTGGCAGATTGGCTACCCAGTTGCTTGATAACTATCTAGCTAATATTGAAgtaggcatctgcaaactc
agctactaatataagcttttgccttaccgccacttgccaaagttggaattgtttttaataaatgttcaatattttgaagcttgtatgtagcctgcaatgtgcctttcctttttactggtgttcatgtagtcgcaatggatttgtcctgcagCCAGTGCTTGATTCTAGGCAAACTGGCCACACGTTGAATGGATGCTTTT
CCCTACTGAAAATCAGTTACAACTCGTCATCGAGGTT

Intron 3 GTTGTTCAAT ... TATGCATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25	6	4	0
A2	14.29	6	0	2
A3	20	4	2	0
A4	50	2	3	1
A5	60	2	2	4
A6	34.62	17	7	11
A7	27.78	13	2	8
A8	17.46	26	6	5
A9	20.93	17	1	8
A10	27.27	4	0	3

>TRINITY_DN35376_c0_g1_i2 from sample A6 / Example spliced isoform

TTTTTATTTCAATGCCCTCCACCGCCGATTGAGCTGTTCATATCGAGATTTCATTTACTAGAAATCCGAGCAAGATCAAAACCTGGTGGTTTTAAAACGCGGTTTAAATCGCCCCAAATTCACATACGACGGCTGGTTTTAAAACTGCTGGTTTCGATTCGTGCGGCTTCTCGTTGGTTGTATAGCAGGCGACGCAAAAT
ACCCGATCGAACAACACCGGCTTCGCAGGGAAGCATTTTGACCCTGTTTCTCACTATTTACCGTCTCATTTTCCTTAACGGTCTTTGACATTTTGCCCTCGCGAGCATGCAGCCCCTCTAAATCACCCACAGTGCAGGTGTTTAAAATGAAAGACGAAAATCAAGAAGCTCAGACTGATTCCACAGATATACCTCCAAAA
CGGAAGCGAGGGCGCCCTCGTAAATCACACGTTTCGTATCCAGGTGAGAAGGCTCATGTACCACAGCGGTTGGATGGGAAAAAGAAACCAAGACGCAAGAAAATAGATTCGTCAACGCCTTCATTTCCTGACAATACTTTTGTAGGCCAATCAGTTCATGGTGTATTGGATGGTTCATTTGATGCTGGCTACTTGCTGAT
GGTTAGAGTGGGAGACACTGATACAGTCCTGCGAGGTGTTGTCTTCGATCCAGGAATGTCAGTGCCTGTTTCTAAAGCAAATGACATTGCACCGCATGTCAATCTGACAAGGAGAGATGAGAACATTCTTCTACCCCCTGAGCTTCCTCCATCTATTCACAATACTGTAGCTATGCCATCAACTCCTGTTCCTTTATTGC
CAAATCTGGTGCCTGTTGTGAATAGTCAGGGAACAACATCAGTTCCTATACCAGATCGATCTGCACCTCCAGTGCAGGTTGGCTCAAATCAAACAAGGGTTGTTTCGGGTCAACCAAATGGGAGCTTACCAAAGTCCGTACCTGATCATTCTAGTCAGATCAACAAAGAGCCTTTCCCAAAGCCAGGAGAATCTTTCTCT
TACTCGCAGCAGTCGATCCCCATTCCCACCGGACCAGTCTCTCGTCCACAGCAGCCAAACCCCATTCTCACCGGATCTGTCTCTCGTCTGCAGCAGCCAATCCCCAGTCTCAGTGAACATGTTTCACACACACAGCAACCAATCCCCACCCCTGCTGAACCTGTTTTTCGTCCAATGCAGTCAATTCCCAAACCTGCTGA
ACCCGTTTCCCATCCACAACATCCAACCCACCCATTTACTGAACCTGTCTCTCACCCACAGTAGCCAATTCCTCACTCTGGATATCCTGTCAAATCATAGATGAGTATTACTGATAAGCAAGCTTTTAAGGGGTCTTGCAAGAAGGCTTGGTTATACATACAATTCATTCTTTGGAGGCTGTGCCCCATATCACTGGGAT
TTGAAGCTAACTCTTGTATTTGAAAGTGGATTTGGAAGAAAGAATGGTGATAATTAGCCACAATCACATGAAGGACACGAGACATTTTCCAGAATTGTTTGTCACAAGCACCCATTCATAAATTACCAGGCATTCAGGGAGCAAACGAAAACACATAAGAAGGGCTGTGAATAGAATTGACTTTGTTTCACAGGAGTTGC
TATCAAGATTGAATCATTTGGTCAATATATAACACTCTTCCTGGCAGATTGGCTACCCAGTTGCTTGATAACTATCTAGCTAATATTGAAGTAGGCATCTGCAAACTCAGCTACTAATATAAGCTTTTGCCTTACCGCCACTTGCCAAAGTTGGAATTGTTTTTAATAAATGTTCAATATTTTGAAGCTTGTATGTAGCC
TGCAATGTGCCTTTCCTTTTTACTGGTGTTCATGTAGTCGCAATGGATTTGTCCTGCAGCCAGTGCTTGATTCTAGGCAAACTGGCCACACGTTGAATGGATGCTTTTCCCTACTGAAAATCAGTTACAACTCGTCATCGAGGTTTTGGTATAATTAGTTCAAACACAATGGTTGTGAAGTAGAACTCGTCTACAGGCAC
AATGCAACATGCAACACAAGGAGTATCACCTGGTTTCAACAGTTTAGAGTAACTATCTTATTTTGAAGTGTACAGAAAGGGCAATAGTACGTCGGTAATATAGAAGCATTGACACTACCCGTAGGAGTATTAAGTGCCGGTGTGAATGACGTC

>TRINITY_DN35376_c0_g1_i4 from sample A6 / Example unspliced isoform:

TGGGAGGATAATATATTGTTTCTCTTCCCTAGTATAATGTTTTGATCCATGAATGCAGGGGTCTTGCAAGAAGGCTTGGTTATACATACAATTCATTCTTTGGAGGCTGTGCCCCATATCACTGGGATTTGAAGCTAACTCTTGTATTTGAAAGTGGATTTGGAAGAAAGAATGGTGATAATTAGgttgttcaatctata
tactcaagtttcccctcaatgaaatttgtttctaacctggatgacttgaatgctgcgtttgttgaagggtttcattcttatattggaattggttggtcctttttcaaatctcacctttttcctttttatgcatcagCCACAATCACATGAAGGACACGAGACATTTTCCAGAATTGTTTGTCACAAGCACCCATTCATAA
ATTACCAGGCATTCAGGGAGCAAACGAAAACACATAAGAAGGGCTGTGAATAGAATTGACTTTGTTTCACAGGAGTTGCTATCAAGATTGAATCATTTGGTCAATATATAACACTCTTCCTGGCAGATTGGCTACCCAGTTGCTTGATAACTATCTAGCTAATATTGAAGTAGGCATCTGCAAACTCAGCTACTAATATA
AGCTTTTGCCTTACCGCCACTTGCCAAAGTTGGAATTGTTTTTAATAAATGTTCAATATTTTGAAGCTTGTATGTAGCCTGCAATGTGCCTTTCCTTTTTACTGGTGTTCATGTAGTCGCAATGGATTTGTCCTGCAGCCAGTGCTTGATTCTAGGCAAACTGGCCACACGTTGAATGGATGCTTTTCCCTACTGAAAAT
CAGTTACAACTCGTCATCGAGGTTTTGGTATAATTAGTTCAAACACAATGGTTGTGAAGTAGAACTCGTCTACAGGCACAATGCAACATGCAACACAAGGAGTATCACCTGGTTTCAACAGTTTAGAGTAACTATCTTATTTTGAAGTGTACAGAAAGGGCAATAGTACGTCGGTAATATAGAAGCATTGACACTACCCG
TAGGAGTATTAAGTGCCGGTGTGAATGACGTC

Intron 4 GTTTGATATT ... TCATATGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.27	118	22	71
A2	26.07	78	12	43
A3	22.39	104	15	45
A4	35.75	62	20	49
A5	40.87	34	7	40
A6	23.23	395	50	189
A7	22.54	555	85	238
A8	22.73	362	53	160
A9	22.91	530	100	215
A10	24.17	425	73	198

>TRINITY_DN96563_c0_g1_i1 from sample A4 / Example spliced isoform

ACGCCGATTGAGCTGTTCATATCGAGATTTCATTTACTAGAAATCCGAGCAAGATCAAAACCTGGTGGTTTTAAAACGCGGTTTAAATCGCCCCAAATTCACATACGACGGCTGGTTTTAAAACTGCTGGTTTCGATTCGTGCGGCTTCTCGTTGGTTGTATAGCAGGCGACGCAAAATACCCGATCGAACAACACCGGC
TTCGCAGGGAAGCATTTTGACCCTGTTTCTCACTATTTACCGTCTCATTTTCCTTAACGGTCTTTGACATTTTGCCCTCGCGAGCATGCAGCCCCTCTAAATCACCCACAGGTGTTTAAAATGAAAGACGAAAATCAAGAAGCTCAGACTGATTCCACAGATATACCTCCAAAACGGAAGCGAGGGCGCCCTCGTAAATC
ACACGTTTCGTATCCAGGTGAGAAGGCTCATGTACCACAGCGGTTGGATGGGAAAAAGAAACCAAGACGCAAGAAAATAGATTCGTCAACGCCTTCATTTCCTGACAATACTTTTGTAGGCCAATCAGTTCATGGTGTATTGGATGGTTCATTTGATGCTGGCTACTTGCTGATGGTTAGAGTGGGAGACACTGATACAG
TCCTGCGAGGTGTTGTCTTCGATCCAGGAATGTCAGTGCCTGTTTCTAAAGCAAATGACATTGCACCGCATGTCAATCTGACAAGGAGAGATGAGAACATTCTTCTACCCCCTGAGCTTCCTCCATCTATTCACAATACTGTAGCTATGCCATCAACTCCTGTTCCTTTATTGCCAAATCTGGTGCCTGTTGTGAATAGT
CAGGGAACAACATCAGTTCCTATACCAGATCGATCTGCACCTCCAGTGCAGGTTGGCTCAAATCAAACAAGGGTTGTTTCGGGTCAACCAAATGGGAGCTTACCAAAGTCCGTACCTGATCATTCTAGTCAGATCAACAAAGAGCCTTTCCCAAAGCCAGGAGAATCTTTCTCTTACTCGCAGCAGTCGATCCCCATTCC
CACCGGACCAGTCTCTCGTCCACAGCAGCCAAACCCCATTCTCACCGGATCTGTCTCTCGTCTGCAGCAGCCAATCCCCAGTCTCAGTGAACATGTTTCACACACACAGCAACCAATCCCCACCCCTGCTGAACCTGTTTTTCGTCCAATGCAGTCAATTCCCAAACCTGCTGAACCCGTTTCCCATCCACAACATCCAA
CCCACCCATTTACTGAACCTGTCTCTCACCCACAGTAGCCAATTCCTCACTCTGGATATCCTGTCAAATCATAGATGAGTATTACTGATAAGCAAGCTTTTAAGGTACTGAATTTGTTCTGTTGACTGGTTATTAGAATGCTTGCCTCTAAATTTTTTGGTAAGCATCATTGTCAACGGCTAATCTATTGAGCATTAGAT
GTGCCTGTTAAGTTTGTTCTTCTAAATGCTACCCAGAGAAGAATATTTTCTGCAAGTTTACTGACTATCTATTTCCAAGATTGGGCGATCTTCTATATCCATGTCTTATAAATGTTGTTATTTTGTTGTGCTCTAATTCCACAAAGTACTATTATT

>TRINITY_DN96563_c0_g1_i2 from sample A4 / Example unspliced isoform:

ACGCCGATTGAGCTGTTCATATCGAGATTTCATTTACTAGAAATCCGAGCAAGATCAAAACCTGGTGGTTTTAAAACGCGGTTTAAATCGCCCCAAATTCACATACGACGGCTGGTTTTAAAACTGCTGGTTTCGATTCGTGCGGCTTCTCGTTGGTTGTATAGCAGGCGACGCAAAATACCCGATCGAACAACACCGGC
TTCGCAGGGAAGCATTTTGACCCTGTTTCTCACTATTTACCGTCTCATTTTCCTTAACGGTCTTTGACATTTTGCCCTCGCGAGCATGCAGCCCCTCTAAATCACCCACAGgtttgatatttatttattattttcccctcttttgtctgtttcatcttcggtaagagatgtaaatctgtacatacgagaaatctttctta
ttgttttcttttctaaaaaccttatgttaccttactgtaatgtatttattttcttcaacggaaattatgttatttacattatttgaaacatcacagtacaaatttgccgcagccattaacagtgcgagacccctttcactgcggagacactgtagtgatagtttagcagactattcggacccgataaagagagggtttta
gagcttacaagctgggttttatggggttttaactatatttcgttggtcacttaatagccctagattgttttagggcaattttttggtcgttagtagaccattattaatatcattgtggttgtgtggcattttgacggctccctcgatttactacagtgatatagttggatatcataagggttgctgtgttgaagcgaggt
tggattttcatatggagGTGTTTAAAATGAAAGACGAAAATCAAGAAGCTCAGACTGATTCCACAGATATACCTCCAAAACGGAAGCGAGGGCGCCCTCGTAAATCACACGTTTCGTATCCAGGTGAGAAGGCTCATGTACCACAGCGGTTGGATGGGAAAAAGAAACCAAGACGCAAGAAAATAGATTCGTCAACGCCT
TCATTTCCTGACAATACTTTTGTAGGCCAATCAGTTCATGGTGTATTGGATGGTTCATTTGATGCTGGCTACTTGCTGATGGTTAGAGTGGGAGACACTGATACAGTCCTGCGAGGTGTTGTCTTCGATCCAGGAATGTCAGTGCCTGTTTCTAAAGCAAATGACATTGCACCGCATGTCAATCTGACAAGGAGAGATGA
GAACATTCTTCTACCCCCTGAGCTTCCTCCATCTATTCACAATACTGTAGCTATGCCATCAACTCCTGTTCCTTTATTGCCAAATCTGGTGCCTGTTGTGAATAGTCAGGGAACAACATCAGTTCCTATACCAGATCGATCTGCACCTCCAGTGCAGGTTGGCTCAAATCAAACAAGGGTTGTTTCGGGTCAACCAAATG
GGAGCTTACCAAAGTCCGTACCTGATCATTCTAGTCAGATCAACAAAGAGCCTTTCCCAAAGCCAGGAGAATCTTTCTCTTACTCGCAGCAGTCGATCCCCATTCCCACCGGACCAGTCTCTCGTCCACAGCAGCCAAACCCCATTCTCACCGGATCTGTCTCTCGTCTGCAGCAGCCAATCCCCAGTCTCAGTGAACAT
GTTTCACACACACAGCAACCAATCCCCACCCCTGCTGAACCTGTTTTTCGTCCAATGCAGTCAATTCCCAAACCTGCTGAACCCGTTTCCCATCCACAACATCCAACCCACCCATTTACTGAACCTGTCTCTCACCCACAGTAGCCAATTCCTCACTCTGGATATCCTGTCAAATCATAGATGAGTATTACTGATAAGCA
AGCTTTTAAGGTACTGAATTTGTTCTGTTGACTGGTTATTAGAATGCTTGCCTCTAAATTTTTTGGTAAGCATCATTGTCAACGGCTAATCTATTGAGCATTAGATGTGCCTGTTAAGTTTGTTCTTCTAAATGCTACCCAGAGAAGAATATTTTCTGCAAGTTTACTGACTATCTATTTCCAAGATTGGGCGATCTTCT
ATATCCATGTCTTATAAATGTTGTTATTTTGTTGTGCTCTAATTCCACAAAGTACTATTATT

Intron 5 GTTTGATATT ... TTTACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.48	62	12	16
A2	17.07	34	6	7
A3	19.61	41	8.5	10
A4	32.6	53.8	10.5	26
A5	35.29	22	4.5	12
A6	24.71	114.3	25.5	37.5
A7	20.11	192.8	42.5	48.5
A8	20.12	134	26.5	33.8
A9	11.37	220.3	50	28.3
A10	19.44	164.8	37.5	39.8

>TRINITY_DN96563_c0_g1_i3 from sample A4 / Example spliced isoform

ACGCCGATTGAGCTGTTCATATCGAGATTTCATTTACTAGAAATCCGAGCAAGATCAAAACCTGGTGGTTTTAAAACGCGGTTTAAATCGCCCCAAATTCACATACGACGGCTGGTTTTAAAACTGCTGGTTTCGATTCGTGCGGCTTCTCGTTGGTTGTATAGCAGGCGACGCAAAATACCCGATCGAACAACACCGGC
TTCGCAGGGAAGCATTTTGACCCTGTTTCTCACTATTTACCGTCTCATTTTCCTTAACGGTCTTTGACATTTTGCCCTCGCGAGCATGCAGCCCCTCTAAATCACCCACAGTGATATAGTTGGATATCATAAGGGTTGCTGTGTTGAAGCGAGGTTGGATTTTCATATGGAGGTGTTTAAAATGAAAGACGAAAATCAAG
AAGCTCAGACTGATTCCACAGATATACCTCCAAAACGGAAGCGAGGGCGCCCTCGTAAATCACACGTTTCGTATCCAGGTGAGAAGGCTCATGTACCACAGCGGTTGGATGGGAAAAAGAAACCAAGACGCAAGAAAATAGATTCGTCAACGCCTTCATTTCCTGACAATACTTTTGTAGGCCAATCAGTTCATGGTGTA
TTGGATGGTTCATTTGATGCTGGCTACTTGCTGATGGTTAGAGTGGGAGACACTGATACAGTCCTGCGAGGTGTTGTCTTCGATCCAGGAATGTCAGTGCCTGTTTCTAAAGCAAATGACATTGCACCGCATGTCAATCTGACAAGGAGAGATGAGAACATTCTTCTACCCCCTGAGCTTCCTCCATCTATTCACAATAC
TGTAGCTATGCCATCAACTCCTGTTCCTTTATTGCCAAATCTGGTGCCTGTTGTGAATAGTCAGGGAACAACATCAGTTCCTATACCAGATCGATCTGCACCTCCAGTGCAGGTTGGCTCAAATCAAACAAGGGTTGTTTCGGGTCAACCAAATGGGAGCTTACCAAAGTCCGTACCTGATCATTCTAGTCAGATCAACA
AAGAGCCTTTCCCAAAGCCAGGAGAATCTTTCTCTTACTCGCAGCAGTCGATCCCCATTCCCACCGGACCAGTCTCTCGTCCACAGCAGCCAAACCCCATTCTCACCGGATCTGTCTCTCGTCTGCAGCAGCCAATCCCCAGTCTCAGTGAACATGTTTCACACACACAGCAACCAATCCCCACCCCTGCTGAACCTGTT
TTTCGTCCAATGCAGTCAATTCCCAAACCTGCTGAACCCGTTTCCCATCCACAACATCCAACCCACCCATTTACTGAACCTGTCTCTCACCCACAGTAGCCAATTCCTCACTCTGGATATCCTGTCAAATCATAGATGAGTATTACTGATAAGCAAGCTTTTAAGGTACTGAATTTGTTCTGTTGACTGGTTATTAGAAT
GCTTGCCTCTAAATTTTTTGGTAAGCATCATTGTCAACGGCTAATCTATTGAGCATTAGATGTGCCTGTTAAGTTTGTTCTTCTAAATGCTACCCAGAGAAGAATATTTTCTGCAAGTTTACTGACTATCTATTTCCAAGATTGGGCGATCTTCTATATCCATGTCTTATAAATGTTGTTATTTTGTTGTGCTCTAATTC
CACAAAGTACTATTATT

>TRINITY_DN96563_c0_g1_i2 from sample A4 / Example unspliced isoform:

ACGCCGATTGAGCTGTTCATATCGAGATTTCATTTACTAGAAATCCGAGCAAGATCAAAACCTGGTGGTTTTAAAACGCGGTTTAAATCGCCCCAAATTCACATACGACGGCTGGTTTTAAAACTGCTGGTTTCGATTCGTGCGGCTTCTCGTTGGTTGTATAGCAGGCGACGCAAAATACCCGATCGAACAACACCGGC
TTCGCAGGGAAGCATTTTGACCCTGTTTCTCACTATTTACCGTCTCATTTTCCTTAACGGTCTTTGACATTTTGCCCTCGCGAGCATGCAGCCCCTCTAAATCACCCACAGgtttgatatttatttattattttcccctcttttgtctgtttcatcttcggtaagagatgtaaatctgtacatacgagaaatctttctta
ttgttttcttttctaaaaaccttatgttaccttactgtaatgtatttattttcttcaacggaaattatgttatttacattatttgaaacatcacagtacaaatttgccgcagccattaacagtgcgagacccctttcactgcggagacactgtagtgatagtttagcagactattcggacccgataaagagagggtttta
gagcttacaagctgggttttatggggttttaactatatttcgttggtcacttaatagccctagattgttttagggcaattttttggtcgttagtagaccattattaatatcattgtggttgtgtggcattttgacggctccctcgatttactacagTGATATAGTTGGATATCATAAGGGTTGCTGTGTTGAAGCGAGGT
TGGATTTTCATATGGAGGTGTTTAAAATGAAAGACGAAAATCAAGAAGCTCAGACTGATTCCACAGATATACCTCCAAAACGGAAGCGAGGGCGCCCTCGTAAATCACACGTTTCGTATCCAGGTGAGAAGGCTCATGTACCACAGCGGTTGGATGGGAAAAAGAAACCAAGACGCAAGAAAATAGATTCGTCAACGCCT
TCATTTCCTGACAATACTTTTGTAGGCCAATCAGTTCATGGTGTATTGGATGGTTCATTTGATGCTGGCTACTTGCTGATGGTTAGAGTGGGAGACACTGATACAGTCCTGCGAGGTGTTGTCTTCGATCCAGGAATGTCAGTGCCTGTTTCTAAAGCAAATGACATTGCACCGCATGTCAATCTGACAAGGAGAGATGA
GAACATTCTTCTACCCCCTGAGCTTCCTCCATCTATTCACAATACTGTAGCTATGCCATCAACTCCTGTTCCTTTATTGCCAAATCTGGTGCCTGTTGTGAATAGTCAGGGAACAACATCAGTTCCTATACCAGATCGATCTGCACCTCCAGTGCAGGTTGGCTCAAATCAAACAAGGGTTGTTTCGGGTCAACCAAATG
GGAGCTTACCAAAGTCCGTACCTGATCATTCTAGTCAGATCAACAAAGAGCCTTTCCCAAAGCCAGGAGAATCTTTCTCTTACTCGCAGCAGTCGATCCCCATTCCCACCGGACCAGTCTCTCGTCCACAGCAGCCAAACCCCATTCTCACCGGATCTGTCTCTCGTCTGCAGCAGCCAATCCCCAGTCTCAGTGAACAT
GTTTCACACACACAGCAACCAATCCCCACCCCTGCTGAACCTGTTTTTCGTCCAATGCAGTCAATTCCCAAACCTGCTGAACCCGTTTCCCATCCACAACATCCAACCCACCCATTTACTGAACCTGTCTCTCACCCACAGTAGCCAATTCCTCACTCTGGATATCCTGTCAAATCATAGATGAGTATTACTGATAAGCA
AGCTTTTAAGGTACTGAATTTGTTCTGTTGACTGGTTATTAGAATGCTTGCCTCTAAATTTTTTGGTAAGCATCATTGTCAACGGCTAATCTATTGAGCATTAGATGTGCCTGTTAAGTTTGTTCTTCTAAATGCTACCCAGAGAAGAATATTTTCTGCAAGTTTACTGACTATCTATTTCCAAGATTGGGCGATCTTCT
ATATCCATGTCTTATAAATGTTGTTATTTTGTTGTGCTCTAATTCCACAAAGTACTATTATT