sp|P92964|SRS31_ARATH ----> Serine/arginine-rich splicing factor RS31

Intron 1 GTAATAAACA ... TTTGGCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.45	49	70	15
A2	52.07	29	50	13
A3	35.35	64	53	17
A4	49.07	41	60	19
A5	38.64	27	19	15
A6	26.61	40	20	9
A7	22.81	88	36	16
A8	39.82	34	26	19
A9	30	70	41	19
A10	20.78	101	39	14

>TRINITY_DN44250_c0_g2_i5 from sample A9 / Example spliced isoform

ATAGGGTTTCAACACAAAAAGAAACACTGGTGTCCAGTGCAAAGCGAGGGTTAGCGATGAAGCCATTGTACTGTGGAAATATCGAGTACGAGGCTCGGCAGTCAGAGATTGAGCGTTTGTTTGGCAAATATGGAAGAGTCGAAAGGGTTGATATGAAATCAGGAATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTT
GTCCATATTCTTTCGTCATGCAATCAATGTTTGTCTTTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAGCATGTCCAGAGATAAAATCCATGACGCATCACTGCAA
TCACAATGGAGCGTAACTGGTGAACGCGCATTTCTGTTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGGTTTGTGCTTGATGCCCCCTAATGCAAGTCTCTACAGCAATGAGTAACAGGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATT
CGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGGCGTCGGCTAAGTGTGGAGTGGAAAAAGCCTGCTGACCGGGGTTTCCGCCGATCTGAGGATGGAAGAAGGTCTGATACAGGTTTGAAGCCAACGAAGACATTATTTGTGATAAACTTTGACTCATACAACACCAGAGCCAGGGACCTTGAAAGGCATTTTGAGCC
CTATGGCAAGGTGC

>TRINITY_DN44250_c0_g2_i2 from sample A9 / Example unspliced isoform:

ATAGGGTTTCAACACAAAAAGAAACACTGGTGTCCAGTGCAAAGCGAGGGTTAGCGATGAAGCCATTGTACTGTGGAAATATCGAGTACGAGGCTCGGCAGTCAGAGATTGAGCGTTTGTTTGGCAAATATGGAAGAGTCGAAAGGGTTGATATGAAATCAGgtaataaacagcattatattcatattcatattcaatat
ctatttgtttatctctcatgtctttgtaatgatatatatacgctgaaacacatgtttacctgagaatattttgtcattcagatggctttggttgctaatggcataaacaatatagatgctatctgtactttgtcaaaaccctaaacttgttatcagttccttttcctaagctgtttaaacccggttaatgtatgcctgaa
caatttatttctgtatttagaacattcttcatattcttgccatctcctctgaaaatttctatctataattagaaaacctaaaatggtcttctcagttcatcctgaaataagatgtttttgcgttggaaaccataggaggatttttcagtttgattttcgaaggcgtaaaagtaccctgtaaagtttcaattatgcatgat
ctattgttctcagagtggtcagtgttttgggtgtttccgatgtacgcttcaacatccatccgtcattaatgtgcgatctgaaaattgtggttacggtagcaaatcggaaggaaatatcaagtatctaacaaaatacagaggcttactgttaaaatccttggtggcagagaagaagttgcaactattatcctcattggaat
atagtttgtaaacaagatttacttccctaatcatgttggatgcatggttcagtcttcattctagctggtaatgcgtaacctctgtggttcatatccaaggctgcaagctaagtttaaaacagcatatttgaaatgtaggcccctgtactcaaaagtttctttggcgcagGAATATTGTAGAGCATCCAAGCTCTTCCTGG
AGATTTTGTCCATATTCTTTCGTCATGCAATCAATGTTTGTCTTTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAGCATGTCCAGAGATAAAATCCATGACGCATC
ACTGCAATCACAATGGAGCGTAACTGGTGAACGCGCATTTCTGTTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGGTT

Intron 2 GTGCTGTATT ... GTTTGTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.11	20	8	15.5
A2	46.02	10	3	9
A3	50.16	20	2	21
A4	61.31	10	1	16
A5	77.5	1	3	3.5
A6	39.06	15	2	11
A7	37.5	21	7	14
A8	58.17	24	11	34
A9	38.22	32	6	21
A10	37.53	18	0	11.5

>TRINITY_DN40870_c1_g2_i6 from sample A6 / Example spliced isoform

CGAATCTTGAGAAGACGTGAGTCGGTGGTTGTGTGAAGGAAGGGATATATAACACTTTTAGAAGCGTAGACGTCCATCATACGTTTTTTCTATTTTATTTCTGTCTGTTATGCGTGTTGCGATCAATCACACGGATCGATTCATTCCGGCTGGCGATCTGTTCTATAGGGTTTCAACACAAAAAGAAACACTGGTGTCCA
GTGCAAAGCGAGGGTTAGCGATGAAGCCATTGTACTGTGGAAATATCGAGTACGAGGCTCGGCAGTCAGAGATTGAGCGTTTGTTTGGCAAATATGGAAGAGTCGACAGGGTTGATATGAAATCAGGAATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTTGTCCATATTCTTTCGTCATGCAATCAATGTTTGTCT
TTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAGCATGTCCAGAGATAAAATCCATGACGCATCACTGCAATCACAATGGAGCGTAACTGGTGAACGCGCATTTCTG
TTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGGTTTGTGCTTGATGCCCCCTAATGCAAGTCTCTACAGCAATGAGTAACAGGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATTCGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGG
CGTCGGCTAAGTGTGGAGTGGAAAAAGCCTGCTGACCGGGGTTTCCGCCGATCTGAG

>TRINITY_DN40870_c1_g2_i8 from sample A6 / Example unspliced isoform:

AATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTTGTCCATATTCTTTCGTCATGCAATCAATGTTTGTCTTTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAG
CATGTCCAGAGATAAAATCCATGACGCATCACTGCAATCACAATGGAGCGTAACTGGTGAACGCGCATTTCTGTTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGGTTTGTGCTTGATGCCCCCTAATGCAAGTCTCTACAGCAATGAGTAACAGgtgctgtattttg
ttctttgtcagagcctgtatatgcaattaattgcacccgagtttcctgcaaatgtggttgcaattcgttttttttctttattgaatctcatttgtttgtatagGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATTCGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGGCGTCGGCTAAG
TGTGGAGTGGAAAAAGCCTGCTGACCGGGGTTTCCGCCGATCTGAG

Intron 3 GTTTGTGCTT ... GTTTGTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.67	23	9	16.7
A2	33.06	15	5	10
A3	31.36	28	3	23
A4	32.22	28	10	16.7
A5	38.21	7	5	3.7
A6	23.86	28	5	13
A7	25.69	55	22	16.3
A8	34.64	46	13	36
A9	29.59	37	8	23.7
A10	17.41	59	12	13

>TRINITY_DN40870_c1_g2_i3 from sample A6 / Example spliced isoform

CGAATCTTGAGAAGACGTGAGTCGGTGGTTGTGTGAAGGAAGGGATATATAACACTTTTAGAAGCGTAGACGTCCATCATACGTTTTTTCTATTTTATTTCTGTCTGTTATGCGTGTTGCGATCAATCACACGGATCGATTCATTCCGGCTGGCGATCTGTTCTATAGGGTTTTCTGATTTTTCATCACTAGGGTTTCAA
CACAAAAAGAAACACTGGTGTCCAGTGCAAAGCGAGGGTTAGCGATGAAGCCATTGTACTGTGGAAATATCGAGTACGAGGCTCGGCAGTCAGAGATTGAGCGTTTGTTTGGCAAATATGGAAGAGTCGACAGGGTTGATATGAAATCAGGAATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTTGTCCATATTCTT
TCGTCATGCAATCAATGTTTGTCTTTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAGCATGTCCAGAGATAAAATCCATGACGCATCACTGCAATCACAATGGAGC
GTAACTGGTGAACGCGCATTTCTGTTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATTCGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGGCGTCGGCTAAGTGTGGAGTGGAAAAA
GCCTGCTGACCGGGGTTTCCGCCGATCTGAG

>TRINITY_DN40870_c1_g2_i8 from sample A6 / Example unspliced isoform:

AATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTTGTCCATATTCTTTCGTCATGCAATCAATGTTTGTCTTTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAG
CATGTCCAGAGATAAAATCCATGACGCATCACTGCAATCACAATGGAGCGTAACTGGTGAACGCGCATTTCTGTTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGgtttgtgcttgatgccccctaatgcaagtctctacagcaatgagtaacaggtgctgtattttg
ttctttgtcagagcctgtatatgcaattaattgcacccgagtttcctgcaaatgtggttgcaattcgttttttttctttattgaatctcatttgtttgtatagGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATTCGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGGCGTCGGCTAAG
TGTGGAGTGGAAAAAGCCTGCTGACCGGGGTTTCCGCCGATCTGAG

Intron 4 GTTTTTCCTT ... ATTCCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.35	82	13	51
A2	30.11	65	4	28
A3	24.42	86.7	17	28
A4	35.63	56	9	31
A5	39.29	34	11	22
A6	22.18	112.3	4	32
A7	32.03	184.7	2	87
A8	23.17	132.7	10	40
A9	25.91	148.7	9	52
A10	32.34	121.3	13	58

>TRINITY_DN44250_c0_g2_i5 from sample A9 / Example spliced isoform

ATAGGGTTTCAACACAAAAAGAAACACTGGTGTCCAGTGCAAAGCGAGGGTTAGCGATGAAGCCATTGTACTGTGGAAATATCGAGTACGAGGCTCGGCAGTCAGAGATTGAGCGTTTGTTTGGCAAATATGGAAGAGTCGAAAGGGTTGATATGAAATCAGGAATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTT
GTCCATATTCTTTCGTCATGCAATCAATGTTTGTCTTTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAGCATGTCCAGAGATAAAATCCATGACGCATCACTGCAA
TCACAATGGAGCGTAACTGGTGAACGCGCATTTCTGTTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGGTTTGTGCTTGATGCCCCCTAATGCAAGTCTCTACAGCAATGAGTAACAGGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATT
CGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGGCGTCGGCTAAGTGTGGAGTGGAAAAAGCCTGCTGACCGGGGTTTCCGCCGATCTGAGGATGGAAGAAGGTCTGATACAGGTTTGAAGCCAACGAAGACATTATTTGTGATAAACTTTGACTCATACAACACCAGAGCCAGGGACCTTGAAAGGCATTTTGAGCC
CTATGGCAAGGTGC

>TRINITY_DN44250_c0_g2_i1 from sample A9 / Example unspliced isoform:

ATAGGGTTTCAACACAAAAAGAAACACTGGTGTCCAGTGCAAAGCGAGGGTTAGCGATGAAGCCATTGTACTGTGGAAATATCGAGTACGAGGCTCGGCAGTCAGAGATTGAGCGTTTGTTTGGCAAATATGGAAGAGTCGAAAGGGTTGATATGAAATCAGGAATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTT
GTCCATATTCTTTCGTCATGCAATCAATGTTTGTCTTTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAGCATGTCCAGAGATAAAATCCATGACGCATCACTGCAA
TCACAATGGAGCGTAACTGGTGAACGCGCATTTCTGTTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGGTTTGTGCTTGATGCCCCCTAATGCAAGTCTCTACAGCAATGAGTAACAGGTGCTGTATTTTGTTCTTTGTCAGAGCCTGTATATGCAATTAATTGCACC
CGAGTTTCCTGCAAATGTGGTTGCAATTCGTTTTTTTTCTTTATTGAATCTCATTTGTTTGTATAGGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATTCGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGGCGTCGGCTAAGTGTGGAGTGGAAAAAGgtttttccttttggttttaaa
tatgcattgggttatgcttgttttctttgtaggcttgacatatccaggaaaggctttaatagattatgttttcagatacaagaattgaggcaactgctattaatactatgccaatataagactttatcgttgtctttgaaaattcctacagCCTGCTGACCGGGGTTTCCGCCGATCTGAGGATGGAAGAAGGTCTGATA
CAGGTTTGAAGCCAACGAAGACATTATTTGTGATAAACTTTGACTCATACAACACCAGAGCCAGGGACCTTGAAAGGCATTTTGAGCCCTATGGCAAGGTGC

Intron 5 GTTTGTGCTT ... TGAGTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.47	23	10	20
A2	30.23	15	3	10
A3	28.21	28	2	20
A4	26.32	28	10	10
A5	26.32	7	4	1
A6	25.33	28	4	15
A7	26.67	55	19	21
A8	26.98	46	10	24
A9	35.09	37	8	32
A10	21.85	59	15	18

>TRINITY_DN40870_c1_g2_i3 from sample A6 / Example spliced isoform

CGAATCTTGAGAAGACGTGAGTCGGTGGTTGTGTGAAGGAAGGGATATATAACACTTTTAGAAGCGTAGACGTCCATCATACGTTTTTTCTATTTTATTTCTGTCTGTTATGCGTGTTGCGATCAATCACACGGATCGATTCATTCCGGCTGGCGATCTGTTCTATAGGGTTTTCTGATTTTTCATCACTAGGGTTTCAA
CACAAAAAGAAACACTGGTGTCCAGTGCAAAGCGAGGGTTAGCGATGAAGCCATTGTACTGTGGAAATATCGAGTACGAGGCTCGGCAGTCAGAGATTGAGCGTTTGTTTGGCAAATATGGAAGAGTCGACAGGGTTGATATGAAATCAGGAATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTTGTCCATATTCTT
TCGTCATGCAATCAATGTTTGTCTTTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAGCATGTCCAGAGATAAAATCCATGACGCATCACTGCAATCACAATGGAGC
GTAACTGGTGAACGCGCATTTCTGTTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATTCGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGGCGTCGGCTAAGTGTGGAGTGGAAAAA
GCCTGCTGACCGGGGTTTCCGCCGATCTGAG

>TRINITY_DN40870_c1_g2_i6 from sample A6 / Example unspliced isoform:

CGAATCTTGAGAAGACGTGAGTCGGTGGTTGTGTGAAGGAAGGGATATATAACACTTTTAGAAGCGTAGACGTCCATCATACGTTTTTTCTATTTTATTTCTGTCTGTTATGCGTGTTGCGATCAATCACACGGATCGATTCATTCCGGCTGGCGATCTGTTCTATAGGGTTTCAACACAAAAAGAAACACTGGTGTCCA
GTGCAAAGCGAGGGTTAGCGATGAAGCCATTGTACTGTGGAAATATCGAGTACGAGGCTCGGCAGTCAGAGATTGAGCGTTTGTTTGGCAAATATGGAAGAGTCGACAGGGTTGATATGAAATCAGGAATATTGTAGAGCATCCAAGCTCTTCCTGGAGATTTTGTCCATATTCTTTCGTCATGCAATCAATGTTTGTCT
TTCGAGAGGATCGGATTCTAGCCTTGTTCGGCAAAGTCGATATACAAATGACATCTTGCATTTTTTCTCACGACTCCATTACTTTTCATACCCCCCATGCCATGTTAAGTATTCAGCCACGCTCAAGCATGTCCAGAGATAAAATCCATGACGCATCACTGCAATCACAATGGAGCGTAACTGGTGAACGCGCATTTCTG
TTCGATGACCCTACAGCCACTTATGGATTAGACTTACCTGAGACCATTTAATACAACATGCAAGgtttgtgcttgatgccccctaatgcaagtctctacagcaatgagtaacagGTTACGCTTTCATCTACATGGAAGATGAACAAGACGCAGAGGACGCAATTCGAGGACTTGACGATTCAAAATTTGGCAGGCAAAGG
CGTCGGCTAAGTGTGGAGTGGAAAAAGCCTGCTGACCGGGGTTTCCGCCGATCTGAG