NA ----> NA

Intron 1 GTCATATCCG ... TTCTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	2	5	5
A2	100	0	0	2
A3	100	0	2	4
A4	100	0	1	5
A5	71.43	1	3	2
A6	68.18	7	10	20
A7	87.88	2	15	14
A8	81.4	4	13	22
A9	85.71	1	5	7
A10	82.86	3	15	14

>TRINITY_DN37098_c0_g1_i7 from sample A6 / Example spliced isoform

TCGAAACGTCGACCTCGTAACTTGGTAACTATCAAGATTCGAAACGTCGACCTCGCAACTTCGTGTAGATTCTACGAGATCAGTTGAAATATAAGATCGCTTTGATATTTTTGATTCAGGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGA
CAGTACTAACAGCCCCCGGTACAGCGTTTTACCCAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATATTACGAGTATGAAGGAGAACACGGAAAACGGACAAAGGGATTCCAGGTTC
CACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGAACACGGAAAACGGACACTACC

>TRINITY_DN37098_c0_g1_i4 from sample A6 / Example unspliced isoform:

TCGAAACGTCGACCTCGTAACTTGGTAACTATCAAGATTCGAAACGTCGACCTCGCAACTTCGTGTAGATTCTACGAGATCAGTTGAAATATAAGATCGCTTTGATATTTTTGATTCAGGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGA
CAGTACTAACAGCCCCCGGTACAGCGTTTTACCCAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGgtcatatccgggcaagattcaatccttccttc
ctttatttcttttcaaactcataatttctttagtttttcatggcttccctcttttgtttcaaacctttaatattgccggtataattacgagtatggaggtcatctccaggcaagattctttccttttctttctttctttccaaactcgtaatttcttttgttttttcatggcttctttcttttcaaactcatattttctt
tagtttttccttttctttctttctttctttccaaactcgtaatttcttttgttttttcatggcttctttcttttcaaactcatattttctttagttttttcatggcttccctctttcttttcaaacctttaatattgccgctataattacgagtatggaggttatctccaggcaggattctttccttttctttctttctt
ttcaaactcttaatttcttttgttttttcatggcttctctctttcttttcaaacctttaatattgctggtaatttattttgtttttctcttttgtcttttcatggcttcccttcctggtccagggaccaatcaatatctacgatccggatgatttactgagattttctttacagAACACGGAAAACGGACACTACCATTT
ATATTTTTTACTGCCAGCATTCCACCAACAGAGCGCCTAAAATTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGAACACGGAAAACGGACACTACCATTTATATTTTTTACTGCCAGCATTCCACCAACAG
AGCGCCTAAAATGCACTTGCTCAATTCTACCACTCTTTACAGCAACATCTGGATCGGGTTCCTTTGGAGACGTACCCTCCCACCTTGGTCTATGTAGCTTCCTGCATCAATCAATACTAG

Intron 2 GTAAGAAAGT ... TTTTTCACAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	2
A2	-1	0.3	0	1
A3	100	0	1	0
A4	62.5	1.5	2	0
A5	62.5	0.8	1	0
A6	36.76	14.3	8	0.5
A7	12.95	6.8	1	0
A8	40.67	7.8	5	2.5
A9	44.44	5	4	0
A10	33.08	8.3	4	0

>TRINITY_DN44184_c1_g2_i8 from sample A8 / Example spliced isoform

GTGGAAACTACGACGCTTCCTTCCAGTCACAAAATGCAAATGCAACCATTCGTTTTCTTGGAGATCTCGCAACTCCCGTCGAGCCGATTATCATTCTGTGCTTCTCAAGCGTCTGATGAATACCTAAAATCATATAAGATCGTTTGTTTCAACTGATGTCGGAGAATCCACAGTAACCAAGTTACGAGGTTATCAGGATT
CGAAACGTCGACCTCGTAACTTGGTAACTATCAAGATTCGAAACGTCGACCTCGCAACTTCGTGTAGATTCTACGAGATCAGTTGAAATATAAGATCGCTTTGATATTTTTGATTCAGGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGAC
AGTACTAACAGCCCCCGGTACAGCGTTTTACCCAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGAACACGGAAAACGGACACTACC
ATTTATATTTTTTACTGCCAGCATTCCACCAACAGAGCGCCTAAAATGATGCGATTATTCATAGAATCAGACGTGCGAAGGAATTTGAAAATTCATCCGGATACAGATTACCCTCATGTGTTCCTCTTGAATGAGGGTTACAACGACATTTATAAACAAAATCTGGAGCTATGTGAACCTCAAGGCTATGTCAAAATGTT
GGATCGTATAATTCAGGAATAGTGTGCATATTATTGTTTCAAGCTTTTTTAGGATTCTAGGTCATTTAGAGAACCCATTAAAGGTGTAAGTATTTCTTTAAAATTTGAAGTTTAAATAAAAGATTAATGGTATACATTTGTGAGTATATTTGGTTATGAATATGAATGGGAGGCCCATCCATGCTAATTAGATTAATTAG
ATTAT

>TRINITY_DN44184_c1_g2_i7 from sample A8 / Example unspliced isoform:

GTGGAAACTACGACGCTTCCTTCCAGTCACAAAATGCAAATGCAACCATTCGTTTTCTTGGAGATCTCGCAACTCCCGTCGAGCCGATTATCATTCTGTGCTTCTCAAGCGTCTGATGAATACCTAAAATCATATAAGATCGTTTGTTTCAACTGATGTCGGAGAATCCACAGTAACCAAGTTACGAGGTTATCAGGATT
CGAAACGTCGACCTCGTAACTTGGTAACTATCAAGATTCGAAACGTCGACCTCGCAACTTCGTGTAGATTCTACGAGATCAGTTGAAATATAAGATCGCTTTGATATTTTTGATTCAGGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGAC
AGTACTAACAGCCCCCGGTACAGCGTTTTACCCAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGGCAAGATTCAATCCTTCCTTCC
TTTATTTCTTTTCAAACTCATAATTTCTTTAGTTTTTCATGGCTTCCCTCTTTTGTTTCAAACCTTTAATATTGCCGGTATAATTACGAGTATGGAGGTCATCTCCAGGCAAGATTCTTTCCTTTTCTTTCTTTCTTTCCAAACTCGTAATTTCTTTTGTTTTTTCATGGCTTCTTTCTTTTCAAACTCATATTTTCTTT
AGTTTTTTCATGGCTTCCCTCTTTCTTTTCAAACCTTTAATATTGCCGCTATAATTACGAGTATGGAGGTTATCTCCAGGCAGGATTCTTTCCTTTTCTTTCTTTCTTTTCAAACTCTTAATTTCTTTTGTTTTTTCATGGCTTCTCTCTTTCTTTTCAAACCTTTAATATTGCTGGTAATTTATTTTGTTTTTCTCTTT
TGTCTTTTCATGGCTTCCCTTCCTGGTCCAGGGACCAATCAATATCTACGATCCGGATGATTTACTGAGATTTTCTTTACAGAACACGGAAAACGGACACTACCATTTATATTTTTTACTGCCAGCATTCCACCAACAGAGCGCCTAAAATgtaagaaagtactgttcttccgatctttaaatctgttctaaaaattgta
caggatatataatttttaatatatttttttcacagGATGCGATTATTCATAGAATCAGACGTGCGAAGGAATTTGAAAATTCATCCGGATACAGATTACCCTCATGTGTTCCTCTTGAATGAGGGTTACAACGACATTTATAAACAAAATCTGGTGTGTATGTCGTTAACATTTTAACTGTAATCTGACTGTCCACAAAA
GGAGCCCTAATTACATATAACTACCAAACACTATGATTATTTCTGCTACATCCCAAACTCAAAAAAGAGTTGAAATCATAGATT

Intron 3 GCAAGATTCA ... TTCTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	2	5	5
A2	50	1	0	2
A3	100	0	2	4
A4	100	0	1	5
A5	55.56	2	3	2
A6	56.19	11	8	20.3
A7	69.95	7	16	16.7
A8	71.61	7	12	23.3
A9	59.44	5	6	8.7
A10	67.4	7	13	16

>TRINITY_DN44184_c1_g2_i8 from sample A8 / Example spliced isoform

GTGGAAACTACGACGCTTCCTTCCAGTCACAAAATGCAAATGCAACCATTCGTTTTCTTGGAGATCTCGCAACTCCCGTCGAGCCGATTATCATTCTGTGCTTCTCAAGCGTCTGATGAATACCTAAAATCATATAAGATCGTTTGTTTCAACTGATGTCGGAGAATCCACAGTAACCAAGTTACGAGGTTATCAGGATT
CGAAACGTCGACCTCGTAACTTGGTAACTATCAAGATTCGAAACGTCGACCTCGCAACTTCGTGTAGATTCTACGAGATCAGTTGAAATATAAGATCGCTTTGATATTTTTGATTCAGGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGAC
AGTACTAACAGCCCCCGGTACAGCGTTTTACCCAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGAACACGGAAAACGGACACTACC
ATTTATATTTTTTACTGCCAGCATTCCACCAACAGAGCGCCTAAAATGATGCGATTATTCATAGAATCAGACGTGCGAAGGAATTTGAAAATTCATCCGGATACAGATTACCCTCATGTGTTCCTCTTGAATGAGGGTTACAACGACATTTATAAACAAAATCTGGAGCTATGTGAACCTCAAGGCTATGTCAAAATGTT
GGATCGTATAATTCAGGAATAGTGTGCATATTATTGTTTCAAGCTTTTTTAGGATTCTAGGTCATTTAGAGAACCCATTAAAGGTGTAAGTATTTCTTTAAAATTTGAAGTTTAAATAAAAGATTAATGGTATACATTTGTGAGTATATTTGGTTATGAATATGAATGGGAGGCCCATCCATGCTAATTAGATTAATTAG
ATTAT

>TRINITY_DN44184_c1_g2_i3 from sample A8 / Example unspliced isoform:

GTGGAAACTACGACGCTTCCTTCCAGTCACAAAATGCAAATGCAACCATTCGTTTTCTTGGAGATCTCGCAACTCCCGTCGAGCCGATTATCATTCTGTGCTTCTCAAGCGTCTGATGAATACCTAAAATCATATAAGATCGTTTGTTTCAACTGATGTCGGAGAATCCACAGTAACCAAGTTACGAGGTTATCAGGATT
CGAAACGTCGACCTCGTAACTTGGTAACTATCAAGATTCGAAACGTCGACCTCGCAACTTCGTGTAGATTCTACGAGATCAGTTGAAATATAAGATCGCTTTGATATTTTTGATTCAGGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGAC
AGTACTAACAGCCCCCGGTACAGCGTTTTACCCAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGgcaagattcaatccttccttcc
tttatttcttttcaaactcataatttctttagtttttcatggcttccctcttttgtttcaaacctttaatattgccggtataattacgagtatggaggtcatctccaggcaagattctttccttttctttctttctttccaaactcgtaatttcttttgttttttcatggcttctttcttttcaaactcatattttcttt
agttttttcatggcttccctctttcttttcaaacctttaatattgccgctataattacgagtatggaggttatctccaggcaggattctttccttttctttctttcttttcaaactcttaatttcttttgttttttcatggcttctctctttcttttcaaacctttaatattgctggtaatttattttgtttttctcttt
tgtcttttcatggcttcccttcctggtccagggaccaatcaatatctacgatccggatgatttactgagattttctttacagAACACGGAAAACGGACACTACCATTTATATTTTTTACTGCCAGCATTCCACCAACAGAGCGCCTAAAATGATGCGATTATTCATAGAATCAGACGTGCGAAGGAATTTGAAAATTCAT
CCGGATACAGATTACCCTCATGTGTTCCTCTTGAATGAGGGTTACAACGACATTTATAAACAAAATCTGGAGCTATGTGAACCTCAAGGCTATGTCAAAATGTTGGATCGTATAATTCAGGAATAGTGTGCATATTATTGTTTCAAGCTTTTTTAGGATTCTAGGTCATTTAGAGAACCCATTAAAGGTGTAAGTATTTC
TTTAAAATTTGAAGTTTAAATAAAAGATTAATGGTATACATTTGTGAGTATATTTGGTTATGAATATGAATGGGAGGCCCATCCATGCTAATTAGATTAATTAGATTAT

Intron 4 GTAACTATCA ... TTTGATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	2	3	5
A2	100	0	2	6
A3	50	9	2	16
A4	100	0	0	1
A5	100	0	3	6
A6	38.46	4	0	5
A7	100	0	3	7
A8	78.26	5	15	21
A9	52.94	8	3	15
A10	64.29	5	3	15

>TRINITY_DN40483_c0_g2_i13 from sample A9 / Example spliced isoform

TCTTTTGTTTTTTCATGGCTTCTTTCTTTTCAAACTCATATTTTCTTTAGTTTTTCCTTTTCTTTCTTTCTTTCTTTCCAAACTCGTAATTTCTTTTGTTTTTTCATGGCTTCTTTCTTTTCAAACTCATATTTTCTTTAGTTTTTGCCGATTATCATTCTGTGCTTCTCAAGCGTCTGATGAATACCTAAAATCATATA
AGATCGTTTGTTTCAACTGATGTCGGAGAATCCACAGTAACCAAGTTACGAGGTTATCAGGATTCGAAACGTCGACCTCGTAACTTGGCCTTTTCTTTCTTTCTTTCTTTCCAAACTCGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGAC
AGTACTAACAGCCCCCGGTACAGCGTTTTACCCAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGGCAAGATTCAATCCTTCCTTCC
TTTATTTCTTTTCAAACTCATAATTTCTTTAGTTTTTCATGGCTTCCCTCTTTCGTTTCAAACCTTTAATATTGCCGGTATAATTACGAGTATGGAGGTTATCTCCAGGCAGGATTCTTTCCTTTTCTTTCTTTCTTTTCAAACTCTTAATTTCTTTTGTTTTTTCATGGCTTCTTTCTTTTCAAACTCATATTTTCTTT
AGTTTTTCCTTTTCTTTCTTTCTTTCTTTCCAAACTCGTAATTTCTTTTGTTTTTTCATGG

>TRINITY_DN40483_c0_g2_i2 from sample A9 / Example unspliced isoform:

TCTTTTGTTTTTTCATGGCTTCTTTCTTTTCAAACTCATATTTTCTTTAGTTTTTCCTTTTCTTTCTTTCTTTCTTTCCAAACTCGTAATTTCTTTTGTTTTTTCATGGCTTCTTTCTTTTCAAACTCATATTTTCTTTAGTTTTTGCCGATTATCATTCTGTGCTTCTCAAGCGTCTGATGAATACCTAAAATCATATA
AGATCGTTTGTTTCAACTGATGTCGGAGAATCCACAGTAACCAAGTTACGAGGTTATCAGGATTCGAAACGTCGACCTCGTAACTTGGCCTTTTCTTTCTTTCTTTCTTTCCAAACTCgtaactatcaagattcgaaacgtcgacctcgcaacttcgtgtagattctacgagatcagttgaaatataagatcgctttgat
atttttgattcagGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGACAGTACTAACAGCCCCCGGTACAGCGTTTTACCCAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGG
GATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGGCAAGATTCAATCCTTCCTTCCTTTATTTCTTTTCAAACTCATAATTTCTTTAGTTTTTCATGGCTTCCCTCTTTCGTTTCAAACCTTTAATATTGCCGGTATAATTACGAGTATGGAGGTTATCTC
CAGGCAGGATTCTTTCCTTTTCTTTCTTTCTTTTCAAACTCTTAATTTCTTTTGTTTTTTCATGGCTTCTTTCTTTTCAAACTCATATTTTCTTTAGTTTTTCCTTTTCTTTCTTTCTTTCTTTCCAAACTCGTAATTTCTTTTGTTTTTTCATGG

Intron 5 GCAAGATTCA ... TTCTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	2	5	5
A2	50	1	0	2
A3	100	0	2	4
A4	100	0	1	5
A5	55.56	2	3	2
A6	68.62	6	8	18.3
A7	79.62	4	16	15.3
A8	77.77	5	12	23
A9	65.18	4	6	9
A10	77.35	4	13	14.3

>TRINITY_DN44492_c0_g1_i2 from sample A7 / Example spliced isoform

AATGCAAATGCAACCATTCGTTTTCTTGGAGATCTCGCAACTCCCGTCGAGCCGATTATCATTCTGTGCTTCTCAAGCGTCTGATGAATACCTAAAATCATATAAGATCGTTTGTTTCAACTGATGTCGGAGAATCCACAGTAACCAAGTTACGAGGTTATCAGGATTCGAAACGTCGACCTCGTAACTTGGTAACTATC
AAGATTCGAAACGTCGACCTCGCAACTTCGTGTAGATTCTACGAGATCAGTTGAAATATAAGATCGCTTTGATATTTTTGATTCAGGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGACAGTACTAACAGCCCCCGGTACAGCGTTTTACC
CAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGAACACGGAAAACGGACACTACCATTTATATTTTTTACTGCCAGCATTCCACCAA
CAGAGCGCCTAAAATAAGTGAAAGTCATTCGCGAAGTCAAAATTGGAGTTTCCGTCAAAGTCCGCAGCGAAAGAAAAGTTTGTGTACTACGCAGTTAATTTTAAAACTAACTGTTGCGGTG

>TRINITY_DN44492_c0_g1_i6 from sample A7 / Example unspliced isoform:

AATGCAAATGCAACCATTCGTTTTCTTGGAGATCTCGCAACTCCCGTCGAGCCGATTATCATTCTGTGCTTCTCAAGCGTCTGATGAATACCTAAAATCATATAAGATCGTTTGTTTCAACTGATGTCGGAGAATCCACAGTAACCAAGTTACGAGGTTATCAGGATTCGAAACGTCGACCTCGTAACTTGGTAACTATC
AAGATTCGAAACGTCGACCTCGCAACTTCGTGTAGATTCTACGAGATCAGTTGAAATATAAGATCGCTTTGATATTTTTGATTCAGGGATCCGGAGAGGCCATCTATTCATTCATTGATTATGAGAATGGAAGCTCCTGCCAATACATTGAAGGCAAATGATTCGGACAGTACTAACAGCCCCCGGTACAGCGTTTTACC
CAACTTTTGGTCTAGATCCAAACTAAAGAGCATTTCGCCGGAAACTTTAAAGAAAGTAATGAACGGCGAAAGGGATTCCAGGTTCCACTGGCTACACACTTATCGATTGCCGCTATAATTACGAGTATGAAGGAGGTCATATCCGGgcaagattcaatccttccttcctttatttcttttcaaactcataatttctttag
tttttcatggcttccctcttttgtttcaaacctttaatattgccggtataattacgagtatggaggtcatctccaggcaagattctttccttttctttctttctttccaaactcgtaatttcttttgttttttcatggcttctttcttttcaaactcatattttctttagtttttccttttctttctttctttctttcca
aactcgtaatttcttttgttttttcatggcttctttcttttcaaactcatattttctttagttttttcatggcttccctctttcttttcaaacctttaatattgccgctataattacgagtatggaggttatctccaggcaggattctttccttttctttctttcttttcaaactcttaatttcttttgttttttcatgg
cttctctctttcttttcaaacctttaatattgctggtaatttattttgtttttctcttttgtcttttcatggcttcccttcctggtccagggaccaatcaatatctacgatccggatgatttactgagattttctttacagAACACGGAAAACGGACACTACCATTTATATTTTTTACTGCCAGCATTCCACCAACAGAG
CGCCTAAAATGCACTTGCTCAATTCTACCACTCTTTACAGCAACATCTGGATCGGGTTCCTTTGGAGACGTACCCTCCCACCTTGGTCTATGTAGCTTCCTGCATCAATCAATACTAGAACACTCTTCAGTGTATTCAACATTTTGCAGCACAATTCAATG