NA ----> NA

Intron 1 GTGCGTTTTG ... TGCCTTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.93	31	24.5	23
A2	47.21	13.8	12.5	14
A3	14.88	61.3	19	6
A4	40.65	34	29.5	21
A5	29.7	22.5	12.5	8.5
A6	15.71	31.5	7	6.5
A7	12.05	198.8	49	15.5
A8	12.25	246.3	57.5	21
A9	12.71	65.8	15.5	8.5
A10	14.7	155.5	47.5	13.5

>TRINITY_DN124383_c6_g2_i4 from sample A1 / Example spliced isoform

AGCTATTGGACCATATATGTGGCTGAATTTCAAGAATGAGGCTACTGACTAAAAATTTTGCGTTGAATTTCAAGATCGCGGGTGTGCGTGAGAAGATGGAGAAGATGGTTATGTTGGTTATGTCAGAAGTAATTGGTGTTGGTGTACATGAAAAAGTGGTTATGCCAGAAGTAATTGGTGTTGGAGTGCATGACAAATGA
AGATGATTGAGGTATATGAAGTGGAGGATGCTTTGACAAAGAATCACACATGAAAAGAAAGATCAGAATGTTTCTTATGTGAAGAAGAAAAAGTAAATGGAGAATACTTGAGGAATTGATGAGAATTCAACCTCATTTTTAAATTGTAAATTAGATTATGGTGTTTTATGTAACACTGCAAACAAATATTTTAATAAAAT
TATCATTTTTTTTTAGAGAAAGGGCAAAAGCCCTTTCTCCAGTCTCTCAGGGATAGCCATTGGCCAAAGCTATCTTTGAGAAG

>TRINITY_DN124383_c6_g2_i3 from sample A1 / Example unspliced isoform:

AGCTATTGGACCATATATGTGGCTGAATTTCAAGAATGAGGCTACTGACTAAAAATTTTGCGTTGAATTTCAAGATCGCGGGTGTGCGTGAGAAGATGGAGAAGATGGTTATGTTGGTTATGTCAGAAGTAATTGGTGTTGGTGTACATGAAAAAGTGGTTATGCCAGAAGTAATTGGTGTTGGAGTGCATGACAAATGA
AGATGATTGAGgtatgttttgagaataaattagttatttattttagattatatttaaaggagattttatttttatttttaataaaaatatttattattaattaattaatttattgccttctagGTATATGAAGTGGAGGATGCTTTGACAAAGAATCACACATGAAAAGAAAGATCAGAATGTTTCTTATGTGAAGAAGA
AAAAGTAAATGGAGAATACTTGAGGAATTGATGAGAATTCAACCTCATTTTTAAATTGTAAATTAGATTATGGTGTTTTATGTAACACTGCAAACAAATATTTTAATAAAATTATCATTTTTTTTTAGAGAAAGGGCAAAAGCCCTTTCTCCAGTCTCTCAGGGATAGCCATTGGCCAAAGCTATCTTTGAGAAG

Intron 2 GTATGTTTTC ... GTATATGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.61	17	10	5
A2	63.16	7	13	11
A3	13.04	10	2	1
A4	92.31	1	15	9
A5	33.33	4	3	1
A6	6.09	54	3	4
A7	8.37	115	9	12
A8	9.09	140	18	10
A9	6.17	76	7	3
A10	8.21	95	10	7

>TRINITY_DN45076_c2_g2_i11 from sample A8 / Example spliced isoform

TGGGTGTGCATGAAAAAGTGGTTATGCCACAAGTAATTGGTGTTGAAGTGCATGACAAATGAAGATGATTGAGTGGATGATGCTTTGACCGAGAATCACACACATGAAAAGAAAAGAAAGATGAGAATGCTTCTTATGTAAAGAAGCAAAAGCAAATGAAGAATCCTCAAGAAATCGATGAGAATTCAACCTCATTTTTA
AATTGTAAATTAGATTATAGTGTTTTATGTAAATTGTAATACTGCAAATAAATATTTTAATAAAATTATCATTTTTTTAGAGAAAGGGCAAAAGCCCTTTTCTCAAATCTCTCAGGGATAGCCATGGCCAAAGCTATCTTTGAGAAGTCTTCTTTACTTAAGTTTTTGCTTATAGATTATTAACTTTCATTTTTTAAATT
TAATGTATGTTGGGTTTTCTAATAATAAAATTGTAAAATTTTCATATATTTTTACAATTATAACATAATAA

>TRINITY_DN45076_c2_g2_i1 from sample A8 / Example unspliced isoform:

ATTTAAAAAAAGAAACTAAAGGCATGTCCAAGTGAAGAGAAAGTAGGTTATGTATGATTTCATTTGGATGTTGTCTTATACAAGTGTGCATGAAAAAGTGATTATGCCACAAGTAATTGGTGTTGAAGTGCATGACAAATGAAGATGATTGAGgtatgttttcagaataagacaagttatttattttagattatatttga
aggaaatttttttattttatttttaataataatatttattattaatttatttatttattggtcttctatgtatatgaagTGGATGATGCTTTGACCGAGAATCACACACATGAAAAGAAAAGAAAGATGAGAATGCTTCTTAT