sp|Q93W57|HSDA_SESIN ----> 11-beta-hydroxysteroid dehydrogenase A

Intron 1 GTTCAATGCG ... ATTGTCTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.89	33	52	70
A2	68.78	32	53	88
A3	64.07	30	58	49
A4	60.53	15	14	32
A5	55.77	23	27	31
A6	64.93	148	202	346
A7	64.2	167	229	370
A8	67.41	160	245	417
A9	59.72	204	240	365
A10	65.8	118	192	262

>TRINITY_DN105523_c2_g1_i1 from sample A5 / Example spliced isoform

TACATGCCGCAGTTGCATGTTGTTTGCCAACCGAGAATGGGATCGAAGTTCAAACATAAATCTTGAATTTCACCCAGATTCGGATCTTGAAGATCAATGGCAGAGCACGAAGATGTCGTCATCATCACAGGATGCACAATCGGAGGCATTGGGCATGCGTTGGCTCTTGCGTTTGCACAACGGAAATGCCGAGTGGTAGC
CACAGCTCGATCCTTGGCATCCATGGAAGGTCTCGACAAGAAGCATCCTGGCATTCACGTAATGGAGCTCGATGTCCGCTCAGATCAGAGCGTGGCCGACGCAGTGAGGGCCGTGGTCGACACGTATGGGCGTATAGACATCCTCATCAATAACGCGGGCGTGCTCTGTGTAGGTCCCCTTGCCGAGCTGCCCTTGTCAG
TCCTCCACTCCACTTTCGATACCAATGTATATGAGTTGAGTTGAAGCCTTTTGGGATAGATGTTATTACAGTGGCCCCAGGGGCAATCAAATCCAATATTGGGTCTAACTGCTCAACTATTTACAAGAATAATCCAGAGTGGAGGTTCTACAAACCTTTTGAAAAGGCAATAAATAAAAGTATTGAATTTTCTCAACAGC
CTGGCAACACTCCAACAGAGGTTTTTGCAAACAAAACCGTGGATGCCATTTTGAGAAGGAAACCACCTGCTTTGTTTTCTTATGGTAAATACTCCACTATTTCAGCCATCTTGTATTATTTACCACTGTGGCTTAAAGATGTGATAATGAGCAAATTATTTATAGTGCCAACTATGGAAAATGCCGAGGAGAAGTAGCAT
CAACAGGCTTCCCATCTTATTAGGAGCTGGTATCACTTGATAAAATGAAATATAAAGTACATTGGCAATTTGGCGCTGAGTTTGATTTTTTAATCTTCATGAACTTTCATTGACAGCTTCGAGCTGATTTATCAGTCTCTGTTTTCTTGCGTCAACATTTCTCGTCACAGTGAGAAGTGAGGTGCTCTGCAGCTCGGATG
GATGTTGATCGTTGTCTCTCTGTGCGTATAATGCTAAAAAAAATGACAAAATAAAAGCTCTCTCTCTCTCACATATAGATGCACATACACATACTTATTAGTATAGATCCATCTTTAACCCTGGATCTTTTAAGCCTAGAGCTTTTTGACCCTTCTAGGTGCTCAACAAAAGGTCTCTCTCTCCC

>TRINITY_DN105523_c2_g1_i2 from sample A5 / Example unspliced isoform:

TACATGCCGCAGTTGCATGTTGTTTGCCAACCGAGAATGGGATCGAAGTTCAAACATAAATCTTGAATTTCACCCAGATTCGGATCTTGAAGATCAATGGCAGAGCACGAAGATGTCGTCATCATCACAGGATGCACAATCGGAGGCATTGGGCATGCGTTGGCTCTTGCGTTTGCACAACGGAAATGCCGAGTGGTAGC
CACAGCTCGATCCTTGGCATCCATGGAAGGTCTCGACAAGAAGCATCCTGGCATTCACGTAATGGAGCTCGATGTCCGCTCAGATCAGAGCGTGGCCGACGCAGTGAGGGCCGTGGTCGACACGTATGGGCGTATAGACATCCTCATCAATAACGCGGGCGTGCTCTGTGTAGGTCCCCTTGCCGAGCTGCCCTTGTCAG
TCCTCCACTCCACTTTCGATACCAATGTATATGgttcaatgcgtgctataacagcggtggtgcctcatatgatatcccaggcgaagggaaaaatagtgaatgttggaagcatcgtggcgttggctcctgtaccgtggggtggtgcttacaatgcttccaaagcagctgttcatgctctatcggattgtctaagAGTTGAG
TTGAAGCCTTTTGGGATAGATGTTATTACAGTGGCCCCAGGGGCAATCAAATCCAATATTGGGTCTAACTGCTCAACTATTTACAAGAATAATCCAGAGTGGAGGTTCTACAAACCTTTTGAAAAGGCAATAAATAAAAGTATTGAATTTTCTCAACAGCCTGGCAACACTCCAACAGAGGTTTTTGCAAACAAAACCGT
GGATGCCATTTTGAGAAGGAAACCACCTGCTTTGTTTTCTTATGGTAAATACTCCACTATTTCAGCCATCTTGTATTATTTACCACTGTGGCTTAAAGATGTGATAATGAGCAAATTATTTATAGTGCCAACTATGGAAAATGCCGAGGAGAAGTAGCATCAACAGGCTTCCCATCTTATTAGGAGCTGGTATCACTTGA
TAAAATGAAATATAAAGTACATTGGCAATTTGGCGCTGAGTTTGATTTTTTAATCTTCATGAACTTTCATTGACAGCTTCGAGCTGATTTATCAGTCTCTGTTTTCTTGCGTCAACATTTCTCGTCACAGTGAGAAGTGAGGTGCTCTGCAGCTCGGATGGATGTTGATCGTTGTCTCTCTGTGCGTATAATGCTAAAAA
AAATGACAAAATAAAAGCTCTCTCTCTCTCACATATAGATGCACATACACATACTTATTAGTATAGATCCATCTTTAACCCTGGATCTTTTAAGCCTAGAGCTTTTTGACCCTTCTAGGTGCTCAACAAAAGGTCTCTCTCTCCC

Intron 2 GTATGTACGC ... AATTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20	52	14	12
A2	13.11	53	12	4
A3	21.09	58	26	5
A4	22.22	14	7	1
A5	16.92	27	7	4
A6	18.38	202	60	31
A7	20.62	229	95	24
A8	19.41	245	78	40
A9	21.7	240	95	38
A10	20.82	192	72	29

>TRINITY_DN119295_c0_g1_i1 from sample A3 / Example spliced isoform

CCAAACCCGATAGGATAAACAACATACATGCCGCAGTTGCATGTTGTTTGCCAACCGAGAATGGGATCGAAGTTCAAACATAAATCTTGAATTTCACCCAGATTCGGATCTTGAAGATCAATGGCAGAGCACGAAGATGTCGTCATCATCACAGGATGCACAATCGGAGGCATTGGGCATGCGTTGGCTCTTGCGTTTGC
ACAACGGAAATGCCGAGTGGTAGCCACAGCTCGATCCTTGGCATCCATGGAAGGTCTCGACAAGAAGCATCCTGGCATTCACGTAATGGAGCTCGATGTCCGCTCAGATCAGAGCGTGGCCGACGCAGTGAGGGCCGTGGTCGACACGTATGGGCGTATAGACATCCTCATCAATAACGCGGGCGTGCTCTGTGTAGGTC
CCCTTGCCGAGCTGCCCTTGTCAGTCCTCCACTCCACTTTCGATACCAATGTATATGGTTCAATGCGTGCTATAACAGCGGTGGTGCCTCATATGATATCCCAGGCGAAGGGAAAAATAGTGAATGTTGGAAGCATCGTGGCGTTGGCTCCTGTACCGTGGGGTGGTGCTTACAATGCTTCCAAAGCAGCTGTTCATGCT
CTATCGGATTGTCTAAGGTTGGTCTAAATATTTTTCCTAAAATAACGTGCCCTTCCCACTGTCTGTCTACTTAGAGGAGCATTGCAATGGATTGTTACTGGCTTACAACACTCGCAAGGTCTTTTATGAGAAGCCACAGGTGAGTATCTATTCTACGAAATCTGGCTAGGATTTGTACCGCATGAAACAACAAATTTGAT
AGGCAAAATGGGCTAGAAGGGGTAAATCTAACTGTAAAACGTGTCTATGGGGATTTGATCCTTGGTACGGTATGAAACACGCAAGCATACGCACAAGACAGAATCCCACCCTATTATGAGCGGAAGCCCCATTTGGAATATTTTGTTTTTCTTAAAACAATTTAGGGAACATTGGGCAATTTTCTCTCCATCTATTTGTT
GCATTCCAACTGCAGATCATTCAC

>TRINITY_DN119295_c0_g1_i3 from sample A3 / Example unspliced isoform:

CCAAACCCGATAGGATAAACAACATACATGCCGCAGTTGCATGTTGTTTGCCAACCGAGAATGGGATCGAAGTTCAAACATAAATCTTGAATTTCACCCAGATTCGGATCTTGAAGATCAATGGCAGAGCACGAAGATGTCGTCATCATCACAGGATGCACAATCGGAGGCATTGGGCATGCGTTGGCTCTTGCGTTTGC
ACAACGGAAATGCCGAGTGGTAGCCACAGCTCGATCCTTGGCATCCATGGAAGGTCTCGACAAGAAGCATCCTGGCATTCACGTAATGGAGCTCGATGTCCGCTCAGATCAGAGCGTGGCCGACGCAGTGAGGGCCGTGGTCGACACGTATGGGCGTATAGACATCCTCATCAATAACGCGGGCGTGCTCTGTGTAGGTC
CCCTTGCCGAGCTGCCCTTGTCAGTCCTCCACTCCACTTTCGATACCAATGTATATGgtatgtacgccgccttccctgaacaaaaacaaatcggtcataagtaccgaaaagaaattaggggaaatatgaatcttgagatatattcaattttacagGTTCAATGCGTGCTATAACAGCGGTGGTGCCTCATATGATATCCC
AGGCGAAGGGAAAAATAGTGAATGTTGGAAGCATCGTGGCGTTGGCTCCTGTACCGTGGGGTGGTGCTTACAATGCTTCCAAAGCAGCTGTTCATGCTCTATCGGATTGTCTAAGGTTGGTCTAAATATTTTTCCTAAAATAACGTGCCCTTCCCACTGTCTGTCTACTTAGAGGAGCATTGCAATGGATTGTTACTGGC
TTACAACACTCGCAAGGTCTTTTATGAGAAGCCACAGGTGAGTATCTATTCTACGAAATCTGGCTAGGATTTGTACCGCATGAAACAACAAATTTGATAGGCAAAATGGGCTAGAAGGGGTAAATCTAACTGTAAAACGTGTCTATGGGGATTTGATCCTTGGTACGGTATGAAACACGCAAGCATACGCACAAGACAGA
ATCCCACCCTATTATGAGCGGAAGCCCCATTTGGAATATTTTGTTTTTCTTAAAACAATTTAGGGAACATTGGGCAATTTTCTCTCCATCTATTTGTTGCATTCCAACTGCAGATCATTCAC