sp|P19242|HSP21_PEA ----> 17.1 kDa class II heat shock protein

Intron 1 GTTGTTTTCT ... TTCTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	5	5
A2	11.86	26	2	5
A3	47.06	18	15	17
A4	48.84	22	22	20
A5	40	12	6	10
A6	20.81	59	4	27
A7	29.55	62	17	35
A8	40.38	31	20	22
A9	31.58	26	9	15
A10	18.64	48	10	12

>TRINITY_DN105248_c0_g1_i4 from sample A4 / Example spliced isoform

CCTGGACGCTTCCAGAATGGATCGAAAGCCTCCATGTATTTCCTCTCCTTCTGCTTCTTCTGCATCGAGTTGTGAAGATCATTTCGCATTCTTACTCTGCAATCAATCTGCTATTTTTCAATCGTTTTTTCCAGAGCGACTGTTCGAGTTAAACTCAGGCTAGCGATTTGCGATTTGAGCAAAGGATTTGAAATTTGGCA
ATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGTGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACTCGCTCTTATGTGCGTGACACCGAGGCCGTGGCTTCGACTCCTGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCCGGCCTTAAATCCAACGACATCAAGGTTCA
AGTGGAGGACGAGAACATTCTGAACATCAGTGGCGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGTGGCGAAGTTCATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGTTCCCAAGCTTC
CCCCGCCAGAGCCGAGGAAACCTAAGACGATTGAAGTCAAAAGTGGATAAATCCTCCGGTGTGTATGCAGAGCTCGAAATCTTTGGTGAATTTTGTGGGGTTTTGTTGATTTTGCCGTAGCTAGATTTTGCACAATTTTGCTTTCAGGTTTGAGAAATATCCATGCAATGCAATCAATCCATCTAACCTGGTGGCGCACG
AATAAAAAGTTTCTATATTTCTGCCATATGTTGAGTTTCCTGTTAATGGCAGAACAAAGCTCTCCATCTAAATATAATTTCCTTATTTGAAAGCTTTTACTGGACATCGCCCGAGGCTTAATTCGTTCTTATTTTGCCCTGAAA

>TRINITY_DN105248_c0_g1_i2 from sample A4 / Example unspliced isoform:

CCTGGACGCTTCCAGAATGGATCGAAAGCCTCCATGTATTTCCTCTCCTTCTGCTTCTTCTGCATCGAGTTGTGAAGATCATTTCGCATTCTTACTCTGCAATCAATCTGCTATTTTTCAATCGTTTTTTCCAGAGCGACTGTTCGAGTTAAACTCAGGCTAGCGATTTGCGATTTGgttgttttcttttttatgttttt
aagcaaatttcttcacaatttgaagcttgtcattgaagattatgcgggcaaataactgtattttctgtgcagAGCAAAGGATTTGAAATTTGGCAATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGTGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACTCGCTCTTATGTGCGTGACACCGAGGCC
GTGGCTTCGACTCCTGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCCGGCCTTAAATCCAACGACATCAAGGTTCAAGTGGAGGACGAGAACATTCTGAACATCAGTGGCGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGTGGCGAAGTT
CATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGTTCCCAAGCTTCCCCCGCCAGAGCCGAGGAAACCTAAGACGATTGAAGTCAAAAGTGGATAAATCCTCCGGTGTGTATGCAGAGCTCGAAATCTTTGGTGAATTTTGTGGGGTTTTG
TTGATTTTGCCGTAGCTAGATTTTGCACAATTTTGCTTTCAGGTTTGAGAAATATCCATGCAATGCAATCAATCCATCTAACCTGGTGGCGCACGAATAAAAAGTTTCTATATTTCTGCCATATGTTGAGTTTCCTGTTAATGGCAGAACAAAGCTCTCCATCTAAATATAATTTCCTTATTTGAAAGCTTTTACTGGAC
ATCGCCCGAGGCTTAATTCGTTCTTATTTTGCCCTGAAA

Intron 2 GTATCTCATA ... TGATGCGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	4.48	64	0	6
A2	9.89	82	16	2
A3	12.95	84	12	13
A4	22.67	87	18	33
A5	15.94	29	8	3
A6	5.82	429	20	33
A7	5.71	429	29	23
A8	5.31	437	16	33
A9	6.43	400	19	36
A10	5.7	405	22	27

>TRINITY_DN105248_c0_g1_i7 from sample A4 / Example spliced isoform

CCTGGACGCTTCCAGAATGGATCGAAAGCCTCCATGTATTTCCTCTCCTTCTGCTTCTTCTGCATCGAGTTGTGAAGAGAATTTCGCATTCTTACTCTGCAATCAGTCTGCTATTCTGCAATCGTTTTTTCCAGAGCTACTGTTCGAGTTAAACTCAGGCTAGCGATTTGCGATTTGGGCAAGAGATTTGAAATTTGGCA
ATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGTGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACTCGCTCTTATGTGCGTGACACCGAGGCCGTGGCTTCGACTCCTGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCCGGCCTTAAATCCAACGACATCAAGGTTCA
AGTGGAGGACGAGAACATTCTGAACATCAGTGGCGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGTGGCGAAGTTCATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGTTCCCAAGCTTC
CCCCGCCGGAGCCGAAGAAACCTAAGGCGATTGAAGTCAAGATTGGATAAACCCTCCACTGTGCATGCAGGGCTAAATTTTGGGGATTATTGTAGGGTTTTTTGCTTTTGCCCTAGCTAGAATTTGCACGATTTTTCTTACCGGAAATATCTATGAAACCTGGTGGCGCACGAATAAATTTTTTTCTGCATTTGTGCCAT
ATGTCGAGCTTACCGTTAACGACAGAACAGAATATAATTTCTGTATTTGGAAGCCTTTGCAAGATCTTTCCCTTGGGTTGACTGAATGTGTTTTTCTGGTCATCTAAATATAATTTGTGTGTTTGAAAA

>TRINITY_DN105248_c0_g1_i5 from sample A4 / Example unspliced isoform:

CCTGGACGCTTCCAGAATGGATCGAAAGCCTCCATGTATTTCCTCTCCTTCTGCTTCTTCTGCATCGAGTTGTGAAGAGAATTTCGCATTCTTACTCTGCAATCAGTCTGCTATTCTGCAATCGTTTTTTCCAGAGCTACTGTTCGAGTTAAACTCAGGCTAGCGATTTGCGATTTGGGCAAGAGATTTGAAATTTGGCA
ATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGTGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACTCGCTCTTATGTGCGTGACACCGAGGCCGTGGCTTCGACTCCTGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCTGGCCTTAAATCCAACGACATCAAGgtatc
tcatagcgcttgaatctccgtcaagatcgctaaattttgccctattttctttcttttgttttggcagtctgctaggattttgttgaaatgtgtgttgatgcgaagGTTCAAGTGGAGGACGAGAACATTCTGAACATCAGTGGCGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACG
CAGAGTGGCGAAGTTCATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGTTCCCAAGCTTCCCCCGCCGGAGCCGAAGAAACCTAAGGCGATTGAAGTCAAGATTGGATAAACCCTCCACTGTGCATGCAGGGCTAAATTTTGGGGATTAT
TGTAGGGTTTTTTGCTTTTGCCCTAGCTAGAATTTGCACGATTTTTCTTACCGGAAATATCTATGAAACCTGGTGGCGCACGAATAAATTTTTTTCTGCATTTGTGCCATATGTCGAGCTTACCGTTAACGACAGAACAGAATATAATTTCTGTATTTGGAAGCCTTTGCAAGATCTTTCCCTTGGGTTGACTGAATGTG
TTTTTCTGGTCATCTAAATATAATTTGTGTGTTTGAAAA

Intron 3 GTTGCCAATT ... TTCTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.33	13	3	1
A2	20	2	0	1
A3	51.72	7	9	6
A4	34.21	25	9	17
A5	36.84	6	1	6
A6	21.02	62	10	23
A7	17.95	48	4	17
A8	25.18	52	7	28
A9	22.89	64	14	24
A10	19.08	53	3	22

>TRINITY_DN105248_c0_g1_i10 from sample A4 / Example spliced isoform

AGCTTCGGCTCGAGAGTCCTGGAAGCTTCGGCTCGAGAGTCCTGGAAGCTTCGGCTCGAGAGTCCTGGACGCTTCCAGAATGGAGGGAAAACCTCTATGTATTTCCTCTGCCTCTGCTTGTTCCGCATCAAATTCTGGAGAGCATTTGGCATTGATACTCTGCAATCGTTTGTTCTAGCGTTACTGTTCTTTAAAATCAG


GGCAAGAGATTTGAAAGATGGCAATGGATCCTTTGTTGAGCACCTTGCAGCAGCTGAGCGTGCCAGACGATCTGGAAAGGCTCCTTAATGCTCCAACTCGCTCTTATGTGCGTGACACTGAAGCCATGGCTTCCACTCCTGTGGATGTTAAAGAATACCCCAACTCTTACGTCTTTATAGTCGACACGCCCGGCCTTAAA
CCGAACGACATCAAGGTTCAAGTGGAGGACGAGAACGTTCTGAACATCAGCGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGCAGCGAAGTTCATGAGGAAGTTCACCCTGCCTGCTGACTCCAACTTCGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGAC
CGTCACCGTCCCCAAGCTTCCCCCGCCGGAGCCGAAGAAACCTAAGGCGATTGAAGTCAAGATTGGATAAACCCTCCACTGTGCATGCAGGGCTAAATTTTGGGGATTATTGTAGGGTTTTTTGCTTTTGCCCTAGCTAGAATTTGCACGATTTTTCTTACCGGAAATATCTATGAAACCTGGTGGCGCACGAATAAATT
TTTTTCTGCATTTGTGCCATATGTCGAGCTTACCGTTAACGACAGAACAGAATATAATTTCTGTATTTGGAAGCCTTTGCAAGATCTTTCCCTTGGGTTGACTGAATGTGTTTTTCTGGTCATCTAAATATAATTTGTGTGTTTGAAAA

>TRINITY_DN105248_c0_g1_i6 from sample A4 / Example unspliced isoform:

AGCTTCGGCTCGAGAGTCCTGGAAGCTTCGGCTCGAGAGTCCTGGAAGCTTCGGCTCGAGAGTCCTGGACGCTTCCAGAATGGAGGGAAAACCTCTATGTATTTCCTCTGCCTCTGCTTGTTCCGCATCAAATTCTGGAGAGCATTTGGCATTGATACTCTGCAATCGTTTGTTCTAGCGTTACTGTTCTTTAAAATCAG


gttgccaatttgtttgcttccagatgttgattttgcaaatttctccacagtttgaagctgctcattaaagaatatgcgggcaaataactgtgttttctgtgcagGGCAAGAGATTTGAAAGATGGCAATGGATCCTTTGTTGAGCACCTTGCAGCAGCTGAGCGTGCCAGACGATCTGGAAAGGCTCCTTAATGCTCCAA
CTCGCTCTTATGTGCGTGACACTGAAGCCATGGCTTCCACTCCTGTGGATGTTAAAGAATACCCCAACTCTTACGTCTTTATAGTCGACACGCCCGGCCTTAAACCGAACGACATCAAGGTATCTCATCAAGCTTAAATCTACTTCAAGATCGTCAAAATTTGCATTATTTTCTGTCTTTTCGTTTGGCCGTCTGCTAGG
GATTTGTTGAAATGTGTGTTGATGTTCCCTGGGAAGGTTCAAGTGGAGGACGAGAACGTTCTGAACATCAGCGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGCAGCGAAGTTCATGAGGAAGTTCACCCTGCCTGCTGACTCCAACTTCGAGGCCATCTCTGCCGC
GTGCCAGGATGGAGTGCTGACCGTCACCGTCCCCAAGCTTCCCCCGCCGGAGCCGAAGAAACCTAAGGCGATTGAAGTCAAGATTGGATAAACCCTCCACTGTGCATGCAGGGCTAAATTTTGGGGATTATTGTAGGGTTTTTTGCTTTTGCCCTAGCTAGAATTTGCACGATTTTTCTTACCGGAAATATCTATGAAAC
CTGGTGGCGCACGAATAAATTTTTTTCTGCATTTGTGCCATATGTCGAGCTTACCGTTAACGACAGAACAGAATATAATTTCTGTATTTGGAAGCCTTTGCAAGATCTTTCCCTTGGGTTGACTGAATGTGTTTTTCTGGTCATCTAAATATAATTTGTGTGTTTGAAAA

Intron 4 GTATCTCATC ... CCCTGGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	6.44	40	2	3.5
A2	0.67	43.5	0.5	0
A3	16.35	45	5	12
A4	17.28	57	10	14
A5	5.69	20.5	2	0.5
A6	9.46	223	14	32
A7	6.01	241	13	17.5
A8	8.86	222	12.5	30.5
A9	12.96	212	27	35.5
A10	6.79	195	5.5	22.5

>TRINITY_DN105248_c0_g1_i10 from sample A4 / Example spliced isoform

AGCTTCGGCTCGAGAGTCCTGGAAGCTTCGGCTCGAGAGTCCTGGAAGCTTCGGCTCGAGAGTCCTGGACGCTTCCAGAATGGAGGGAAAACCTCTATGTATTTCCTCTGCCTCTGCTTGTTCCGCATCAAATTCTGGAGAGCATTTGGCATTGATACTCTGCAATCGTTTGTTCTAGCGTTACTGTTCTTTAAAATCAG
GGCAAGAGATTTGAAAGATGGCAATGGATCCTTTGTTGAGCACCTTGCAGCAGCTGAGCGTGCCAGACGATCTGGAAAGGCTCCTTAATGCTCCAACTCGCTCTTATGTGCGTGACACTGAAGCCATGGCTTCCACTCCTGTGGATGTTAAAGAATACCCCAACTCTTACGTCTTTATAGTCGACACGCCCGGCCTTAAA
CCGAACGACATCAAGGTTCAAGTGGAGGACGAGAACGTTCTGAACATCAGCGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGCAGCGAAGTTCATGAGGAAGTTCACCCTGCCTGCTGACTCCAACTTCGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGAC
CGTCACCGTCCCCAAGCTTCCCCCGCCGGAGCCGAAGAAACCTAAGGCGATTGAAGTCAAGATTGGATAAACCCTCCACTGTGCATGCAGGGCTAAATTTTGGGGATTATTGTAGGGTTTTTTGCTTTTGCCCTAGCTAGAATTTGCACGATTTTTCTTACCGGAAATATCTATGAAACCTGGTGGCGCACGAATAAATT
TTTTTCTGCATTTGTGCCATATGTCGAGCTTACCGTTAACGACAGAACAGAATATAATTTCTGTATTTGGAAGCCTTTGCAAGATCTTTCCCTTGGGTTGACTGAATGTGTTTTTCTGGTCATCTAAATATAATTTGTGTGTTTGAAAA

>TRINITY_DN105248_c0_g1_i6 from sample A4 / Example unspliced isoform:

AGCTTCGGCTCGAGAGTCCTGGAAGCTTCGGCTCGAGAGTCCTGGAAGCTTCGGCTCGAGAGTCCTGGACGCTTCCAGAATGGAGGGAAAACCTCTATGTATTTCCTCTGCCTCTGCTTGTTCCGCATCAAATTCTGGAGAGCATTTGGCATTGATACTCTGCAATCGTTTGTTCTAGCGTTACTGTTCTTTAAAATCAG
GTTGCCAATTTGTTTGCTTCCAGATGTTGATTTTGCAAATTTCTCCACAGTTTGAAGCTGCTCATTAAAGAATATGCGGGCAAATAACTGTGTTTTCTGTGCAGGGCAAGAGATTTGAAAGATGGCAATGGATCCTTTGTTGAGCACCTTGCAGCAGCTGAGCGTGCCAGACGATCTGGAAAGGCTCCTTAATGCTCCAA
CTCGCTCTTATGTGCGTGACACTGAAGCCATGGCTTCCACTCCTGTGGATGTTAAAGAATACCCCAACTCTTACGTCTTTATAGTCGACACGCCCGGCCTTAAACCGAACGACATCAAGgtatctcatcaagcttaaatctacttcaagatcgtcaaaatttgcattattttctgtcttttcgtttggccgtctgctagg
gatttgttgaaatgtgtgttgatgttccctgggaagGTTCAAGTGGAGGACGAGAACGTTCTGAACATCAGCGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGCAGCGAAGTTCATGAGGAAGTTCACCCTGCCTGCTGACTCCAACTTCGAGGCCATCTCTGCCGC
GTGCCAGGATGGAGTGCTGACCGTCACCGTCCCCAAGCTTCCCCCGCCGGAGCCGAAGAAACCTAAGGCGATTGAAGTCAAGATTGGATAAACCCTCCACTGTGCATGCAGGGCTAAATTTTGGGGATTATTGTAGGGTTTTTTGCTTTTGCCCTAGCTAGAATTTGCACGATTTTTCTTACCGGAAATATCTATGAAAC
CTGGTGGCGCACGAATAAATTTTTTTCTGCATTTGTGCCATATGTCGAGCTTACCGTTAACGACAGAACAGAATATAATTTCTGTATTTGGAAGCCTTTGCAAGATCTTTCCCTTGGGTTGACTGAATGTGTTTTTCTGGTCATCTAAATATAATTTGTGTGTTTGAAAA

Intron 5 GTGTCTCATA ... CCCTGCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.21	13	0	7
A2	3.57	27	0	2
A3	11.48	27	1	6
A4	17.39	38	5	11
A5	11.76	15	3	1
A6	7.69	126	1	20
A7	10.4	155	20	16
A8	8.06	154	7	20
A9	11.19	127	17	15
A10	9.16	124	13	12

>TRINITY_DN105248_c0_g1_i13 from sample A4 / Example spliced isoform

AGAGCATTTCGAATTCATACTGTGCTATTCTGCAATTCTTTTATCTGAAGCTACTGTTCGTTAAAATCATAATCAGGGCAAGATATTTGAAAGATGGCAATGGATCCTATGTTGAGCACCGTGCAGCAGCTGTTCGGCGTACCGGACGACCTGGAGAGGCTCCTTAACACCCCGACTCGCTCTTATGTGCGTGATACCGA
GGCCATGGCTTCGACTCCCGTGGATGTAAAAGAATACCCCAACTCTTATGTCTTTATAGTCGACATGCCCGGCCTTAAATCCAACGACATCAAGGTTCAAGTGGAGGACGAGAACATTCTGAACATCAGTGGCGAGCGGAAGAGGAACGAAAAGGAAGAAG

>TRINITY_DN105248_c0_g1_i8 from sample A4 / Example unspliced isoform:

AGAGCATTTCGAATTCATACTGTGCTATTCTGCAATTCTTTTATCTGAAGCTACTGTTCGTTAAAATCATAATCAGGGCAAGATATTTGAAAGATGGCAATGGATCCTATGTTGAGCACCGTGCAGCAGCTGTTCGGCGTACCGGACGACCTGGAGAGGCTCCTTAACACCCCGACTCGCTCTTATGTGCGTGATACCGA
GGCCATGGCTTCGACTCCCGTGGATGTAAAAGAATACCCCAACTCTTATGTCTTTATAGTCGACATGCCCGGCCTTAATTCCAACGACATCAAGgtgtctcataaagcttaaatctccttcaagatcgtcaaaatttgcctttagtttctttccttccaggttatcaaattttgtctttttttttctgccttggccttag
ctagggttggggttttgttgatatgtgtgttgctgtgccctgcgcagGTTCAAGTGGAGGACGAGAATATTCTGAACATCAGTGGCGAGCGGAAGAGGAACGAAAAGGAAGAAGAAGGGGAAGTGAAATACATTCGCATGGAGCGCAGGGTGGGAAAGTTCATGAGGAAATTCACCTTGCCTGCTGACTGCAACCTGGAG
GCCATTTCTGCCGCATATCAGGATGGAGTGCTGACCGTCACTGTTCACAAGCTTCCTCCGCCGGAGCCGAAGAAACCTAAGACGATTGAAGTCAAACTTGGATAAAGCCTGCACTGCGTATGCAGAGTTCGAAATCTTTGTTGAATTTTGTAGCGTTTTGTTGCCTGTGTCCTAGCTAGATTTTGCATGTTTTTGCTTGC
CGGCGTGAGAAATATGTAATGTTATGCAATCTATGTATCCAACATGAAGCGCGTGAATAAAGAATTTTCTGCATTTCTGCCATATGCTGAGCTTCCTGTTCCGCCAGGAACAGAGCTCTGCATCTCTCGTCATACTTTGATTGATTTATGTGTTTTCCTTGTTATCTAAATATAATTTCGGTATTTGAAAGCTTACACTG
GACCTTGCCCATGGTTTAATTTGTTCTGATTTGCTTGTGGAGTTCGAATCTGAACGTTCTCGGTATGATTCTTAAAATAATTTTCTTTTTCCAAAAATAATTTGGGGTTTAATTTTTAAAATCACAGAAGTGGTATGTAGATTGGGAATCCATGGCCCACTGGTAAGATTTTGGGATTTGAACAAGGG