NA ----> NA

Intron 1 GTTTGCTGAA ... TGATTAGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.38	21	1	11
A2	33.33	22	2.5	11
A3	41.38	34	6	24
A4	26.47	25	9	9
A5	40	18	1	12
A6	24.59	184	14.5	60
A7	20	172	16.5	43
A8	30.48	73	14.5	32
A9	21.8	165	14.5	46
A10	25.57	131	15.5	45

>TRINITY_DN101912_c5_g2_i2 from sample A4 / Example spliced isoform

CTCAAAGTCAGAAGAACATTGTTACATTCAATCCTTGCAGCTCGATGGCACGAAAGTTTGAATTCTATTAATCACTGCATATATGTCATTTGAAAAGCTAGTCGTGTATAATCTTTCTGCACCAAGCCGTGTTTCAAGATGGGCACATCAGATTCCAAGCTCAATATCTCTCGGAGTCCCGCAGATGAAATTACCACAGT
TAAGGGCCGAACCGAGGAAACGGATCCCTGCTTGGAGAAGTTGAAATCCCTTAAAATTGTTTCTCCTATACTCAAGTCCCCACCAACAGAAACCACCTTGACTGACATTCTGGTTCGGAAGCCTTCATCGGCCTCAGATTCTGGTGCTTTGGATCCCAAGACTGTGTTTGAACTCTTTTCCCTTTATCGAGAATGGCAGG
AAGACACAGCAAAAAGAATCAACCAAACTCAGGGGGAGTTGGAACATAAAATAGAGATTGTAGATGCATTGGCTGTTAAACTTCTGCAGCGTTTCAATGACTCAGTTTCTGTTATGAAAAGTAGTGCCACACACTTAGAAAATGTCTATCCATTGCAGGTTGAAGTTGGTGAATTAAAAGGGAAGTTGAAAGAGGTCCTT
GCAAATTGTGACGCTCTTTGCAAAAGGATCAACGTAGATGGACCAGATGCGCTACGAGGTAGTCTGATTCCATTTTCGGCAATTGATCTACAGGTCCGCAAGTCTGCTGCTGTTCAGAGAGCTCCATGATTTCTGTTGACAATTATTTTCAAAAAAGTAGATTGTGAAGTGACATTGGCAATCCCTCTTAGTTCCTCCAA
GGTCTTACTTGAGCTTTAGGACTATCTCTAGAGGTGAGAGTTTGAGAGGTAACAGAACCTATCAGAAATTGTGTTTTACTTTAAATTGTTTTATTATGAAAGGCACTATGCAAAAATTGACAATTTTTTTGTTAAAAACTCATTAATTTTGTCAGAAGTGATGACATTTATGACATTTTTGGACAAATTGTAATCGTTTG
AATGACAACAATTTGTTATATATAAGGGTTAAATCTTCATTCTCTGCCACGATCAATCTTAGATTGAGTCTGGTATTTATAAACCCAC

>TRINITY_DN101912_c5_g2_i1 from sample A4 / Example unspliced isoform:

CTCAAAGTCAGAAGAACATTGTTACATTCAATCCTTGCAGCTCGATGGCACGAAAGTTTGAATTCTATTAATCACTGCATATATGTCATTTGAAAAGCTAGTCGTGTATAATCTTTCTGCACCAAGCCGTGTTTCAAGATGGGCACATCAGATTCCAAGCTCAATATCTCTCGGgtttgctgaattattatgctctttga
acggtcgttcgttaactattattcttatttactggtgaggttctgagtttttccattttttttgcatgttggtgattagtagAGTCCCGCAGATGAAATTACCACAGTTAAGGGCCGAACCGAGGAAACGGATCCCTGCTTGGAGAAGTTGAAATCCCTTAAAATTGTTTCTCCTATACTCAAGTCCCCACCAACAGAAA
CCACCTTGACTGACATTCTGGTTCGGAAGCCTTCATCGGCCTCAGATTCTGGTGCTTTGGATCCCAAGACTGTGTTTGAACTCTTTTCCCTTTATCGAGAATGGCAGGAAGACACAGCAAAAAGAATCAACCAAACTCAGGGGGAGTTGGAACATAAAATAGAGATTGTAGATGCATTGGCTGTTAAACTTCTGCAGCGT
TTCAATGACTCAGTTTCTGTTATGAAAAGTAGTGCCACACACTTAGAAAATGTCTATCCATTGCAGGTTGAAGTTGGTGAATTAAAAGGGAAGTTGAAAGAGGTCCTTGCAAATTGTGACGCTCTTTGCAAAAGGATCAACGTAGATGGACCAGATGCGCTACGAGGTAGTCTGATTCCATTTTCGGCAATTGATCTACA
GGTCCGCAAGTCTGCTGCTGTTCAGAGAGCTCCATGATTTCTGTTGACAATTATTTTCAAAAAAGTAGATTGTGAAGTGACATTGGCAATCCCTCTTAGTTCCTCCAAGGTCTTACTTGAGCTTTAGGACTATCTCTAGAGGTGAGAGTTTGAGAGGTAACAGAACCTATCAGAAATTGTGTTTTACTTTAAATTGTTTT
ATTATGAAAGGCACTATGCAAAAATTGACAATTTTTTTGTTAAAAACTCATTAATTTTGTCAGAAGTGATGACATTTATGACATTTTTGGACAAATTGTAATCGTTTGAATGACAACAATTTGTTATATATAAGGGTTAAATCTTCATTCTCTGCCACGATCAATCTTAGATTGAGTCTGGTATTTATAAACCCAC

Intron 2 GTTTGCTGAA ... TGATTAGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.38	21	1.3	11
A2	33.33	22	3.3	11
A3	41.38	34	7.7	24
A4	26.47	25	8.3	9
A5	40	18	1.3	12
A6	24.66	183.3	18.7	60
A7	20	172	19.3	43
A8	30.48	73	16.7	32
A9	21.87	164.3	18.7	46
A10	25.57	131	18.3	45

>TRINITY_DN120079_c2_g2_i2 from sample A3 / Example spliced isoform

TATATATAGATATTAACCATTAAAACATTTTAGCCTTCCTCTCAAAGTCAGAAGAACATTGTTACATTCAATCCTTGCAGCTCGATGGCACGAAAGTTTGAATTCTATTAATCACTGCATGTATGTCAGTTGAAAAGCTAGTCGTGTATAATCTTTCTGCACCAAGCCGTGTTTCAAGATGGGCACATCAGATTCCAAGC
TCAATATCTCTCGGAGTCCCGCAGATGAAATTACCACAGTTAAGGGCCGAACCGAGGAAACGGATCCCTGCTTGGAGAAGTTGAAATCCCTTAAAATTGTTTCTCCTATACTCAAGTCCCCACCAACAGAAACCACCTTGACTGACATTCTGGTTCGGAAGCCTTCATCGGCCTCAGATTCTGGTGCTTTGGATCCCAAG
ACTGTGTTTGAACTCTTTTCCCTTTATCGAGAATGGCAGGAAGACACAGCAAAAAGAATCAACCAAACTCAGGGGGAGTTGGAACATAAAATAGAGATTGTAGATGCATTGGCTGTTAAACTTCTGCAGCGTTTCAATGACTCAGTTTCTGTTATGAAAAGTAGTGCCACACACTTAGAAAATGGTGAGACATGTAAATG
GATTATATTATGCTCC

>TRINITY_DN120079_c2_g2_i6 from sample A3 / Example unspliced isoform:

TATATATAGATATTAACCATTAAAACATTTTAGCCTTCCTCTCAAAGTCAGAAGAACATTGTTACATTCAATCCTTGCAGCTCGATGGCACGAAAGTTTGAATTCTATTAATCACTGCATGTATGTCAGTTGAAAAGCTAGTCGTGTATAATCTTTCTGCACCAAGCCGTGTTTCAAGATGGGCACATCAGATTCCAAGC
TCAATATCTCTCGGgtttgctgaattattatgctctttgaacggtcgttcgttaactattattcttatttactcttactggtgaggttctgagtttttccaatttttttgcatgttggtgattagtagAGTCCCGCAGATGAAATTACCACAGTTAAGGGCCGAACCGAGGAAACGGATCCCTGCTTGGAGAAGTTGAAA
TCCCTTAAAATTGTTTCTCCTATACTCAAGTCCCCACCAACAGAAACCACCTTGACTGACATTCTGGTTCGGAAGCCTTCATCGGCCTCAGATTCTGGTCAGTATCGACAACCGACACAATGGGTTTTTAAATCTATGATTTCCCGATCAGTATCATCCACGTTTTTCAACCTTGACACATTCTGTCATTTTTACATGTG
TTATTTTTTTAAACTATTGGGCCTTACATGTCATGATTTGGGATATTTACTTAATCTGAGCGACGTTTGTTTTCCTTAGTTGTTTTGGAC