sp|Q96453|1433D_SOYBN ----> 14-3-3-like protein D

Intron 1 GTACATGTAG ... TGCTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.5	97	121	131
A2	58.8	103	156	138
A3	49.61	130	112	144
A4	54.69	58	74	66
A5	64.96	24	47	42
A6	57.29	520	605	790
A7	58.84	403	498	654
A8	52.7	491	484	610
A9	57.22	376	451	555
A10	55.98	274	301	396

>TRINITY_DN105406_c0_g2_i3 from sample A5 / Example spliced isoform

CGCCGACGTCTCTGGGGCCCGTAGGTTAAGCCATCGACGCCACACCATTGCAATTATAATACCCCAAACCATTTGTTTATAGCGCAGTGTTCTTCTTCCGCTGCTCTCTCGCTCTCGTGGCACCCGAAGAGAAGTTTCAGAGAGGCCAGGCCAGTCCAGTCTGTGGATCTCTATTCGGACAAGATGAGTAGCGACAAAGA
GCGTGAGACTCATGTTTACATGGCGAAACTCGCTGAACAGGCCGAGCGATACGACGAAATGGTGGAAGCCATGAAGAGGGTAGCAAAGCTGGACGTGGAGTTAACAGTAGAAGAAAGGAATCTACTTTCGGTTGGGTACAAAAATGTGATTGGGGCTCGGCGTGCTTCATGGAGAATAATGTCTAGCATTGAGCAGAAGG
AGGAGGCCAAGGGCAATGATCATAATGTGAAGCGTATCAGAGAATATAGACAGAAAGTAGAAGCGGAGCTTTCTAAGATCTGCCATGATATTATGACTATCATTGATGAACACCTCATTCCTTCCTCCAGTATTGGCGAATCTACTGTTTTTTACTATAAAATGAAAGGAGACTACTACCGTTATCTTGCTGAATTCAAA
ACAGGAAATGAGAGGAAAGAAGCCGCTGATCAGTCCTTGAAAGCTTACCAGACGGCTTCAAGTACCGCAGAGTCGGATTTACCGCCAACTCATCCAATCAGGCTTGGATTGGCCCTGAACTTCTCTGTTTTCTACTATGAAATAATGAATTCACCTGAAAGGGCCTGCCATCTGGCCAAGCAAGCTTTTGACGAAGCTAT
TGCAGAACTTGACACTTTGAGTGAAGAGTCATACAAAGATAGCACTTTGATCATGCAGCTTTTGAGGGACAACCTCACTTTGTGGACCTCCGATCTTCAGGAAGATGGAGTTGAAGATCAGGCCAAGGGGGATGATCCTGCCGTAGGCATGGATGAGGAGCTTTAAGCAGCTGCGATTTACAAATTCTCTCTTTATCTTT
GTTATTCACGCCTGGTTTTGTTTATAAGTTTTAGATTGGAACAAAAGACCAGTAGGACATTGTGGGTCTTAACATGGCGTGTATGCCATGGTTATTAAACGCTTCAATATCTATTAGAGGCCCAGCATTGCAGTACTGTGTATGAATTTAGAGTCTTTGGTTGTGCATTGTCATCGTTTTGAAGATTAATATAGGCCTCT
GATGGATGAAGTGGCTTTGTTGCCACAGAGAAGCTGAATTCTTTGAGCTTTCCTCATTTTCTTTTTCAACTTTTCTCGAAGTTACATGGAATATAGCGGAATAAAAAGGAAATCTTGTATAGATTGCAGGATGTTAGATTAACTTCTCCTGGTATTGAAAAGTGAAGTGTGCAAAGAGATTGACTGTAATTTGATTGCCT
TGTGCCTTAATAGGCATTAGCATTGTTTCAGTTATGAGTTGCAACAATGTTATTTATGTTATGCACAAATATACGTTGCATCGAAGGGAATCCTTAAAATCAAATTGGCA

>TRINITY_DN105406_c0_g2_i4 from sample A5 / Example unspliced isoform:

CGCCGACGTCTCTGGGGCCCGTAGGTTAAGCCATCGACGCCACACCATTGCAATTATAATACCCCAAACCATTTGTTTATAGCGCAGTGTTCTTCTTCCGCTGCTCTCTCGCTCTCGTGGCACCCGAAGAGAAGTTTCAGAGAGGCCAGGCCAGTCCAGTCTGTGGATCTCTATTCGGACAAGATGAGTAGCGACAAAGA
GCGTGAGACTCATGTTTACATGGCGAAACTCGCTGAACAGGCCGAGCGATACGACGAAATGGTGGAAGCCATGAAGAGGGTAGCAAAGCTGGACGTGGAGTTAACAGTAGAAGAAAGGAATCTACTTTCGGTTGGGTACAAAAATGTGATTGGGGCTCGGCGTGCTTCATGGAGAATAATGTCTAGCATTGAGCAGAAGG
AGGAGGCCAAGGGCAATGATCATAATGTGAAGCGTATCAGAGAATATAGACAGAAAGTAGAAGCGGAGCTTTCTAAGATCTGCCATGATATTATGACTATCATTGATGAACACCTCATTCCTTCCTCCAGTATTGGCGAATCTACTGTTTTTTACTATAAAATGAAAGGAGACTACTACCGTTATCTTGCTGAATTCAAA
ACAGGAAATGAGAGGAAAGAAGCCGCTGATCAGTCCTTGAAAGCTTACCAGACGGCTTCAAGTACCGCAGAGTCGGATTTACCGCCAACTCATCCAATCAGGCTTGGATTGGCCCTGAACTTCTCTGTTTTCTACTATGAAATAATGAATTCACCTGAAAGGGCCTGCCATCTGGCCAAGCAAGCTTTTGACGAAGCTAT
TGCAGAACTTGACACTTTGAGTGAAGAGTCATACAAAGATAGCACTTTGATCATGCAGCTTTTGAGGGACAACCTCACTTTGTGGACCTCCGATCTTCAGGAAGATGGAGTTGAAGATCAGGCCAAGGGGGATGATCCTGCCGTAGGCATGGATGAGGAGCTTTAAGCAGgtacatgtagaaacaaatgaagttgattag
atttatgggcttgtatgttcgcctcgatgtattctagagcaggaatctgattatcctttctgctctgcagCTGCGATTTACAAATTCTCTCTTTATCTTTGTTATTCACGCCTGGTTTTGTTTATAAGTTTTAGATTGGAACAAAAGACCAGTAGGACATTGTGGGTCTTAACATGGCGTGTATGCCATGGTTATTAAAC
GCTTCAATATCTATTAGAGGCCCAGCATTGCAGTACTGTGTATGAATTTAGAGTCTTTGGTTGTGCATTGTCATCGTTTTGAAGATTAATATAGGCCTCTGATGGATGAAGTGGCTTTGTTGCCACAGAGAAGCTGAATTCTTTGAGCTTTCCTCATTTTCTTTTTCAACTTTTCTCGAAGTTACATGGAATATAGCGGA
ATAAAAAGGAAATCTTGTATAGATTGCAGGATGTTAGATTAACTTCTCCTGGTATTGAAAAGTGAAGTGTGCAAAGAGATTGACTGTAATTTGATTGCCTTGTGCCTTAATAGGCATTAGCATTGTTTCAGTTATGAGTTGCAACAATGTTATTTATGTTATGCACAAATATACGTTGCATCGAAGGGAATCCTTAAAAT
CAAATTGGCA

Intron 2 GTACATGTAG ... TTGTTTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.49	46	121	134
A2	65.37	67	156	97
A3	67.31	59	112	131
A4	65.25	41	74	80
A5	73.11	16	47	40
A6	70.45	289	605	773
A7	71.32	217	498	581
A8	68.19	271	484	678
A9	66.48	267	451.2	608
A10	64.95	184	301	381

>TRINITY_DN99444_c0_g2_i3 from sample A4 / Example spliced isoform

CTGCACGTTCTTGGACGGTGAGGGGCGGGGCCCTATGGCTGGATTCCTCCCCAACGCCGACGTCTCTGGGGCCCGTAGGTTAAGCCATCGACGCCACACCATTGCAATTATAATACCCCAGGCCAATTGTTTATAGCGCAGTGTTCTTCTTCCGCTGCTCTCTCGCTCTCGTGGCACCCGAAGAGAAGTTTCAGAGAGGC
CAGGCCAGTCCAGTCTGTGGATCTCTATTCGGACAAGATGAGTAGCGACAAAGAGCGTGAGACTCATGTTTACATGGCGAAACTCGCTGAACAGGCCGAGCGATACGACGAAATGGTGGAAGCCATGAAGAGGGTAGCAAAGCTGGACGTGGAGTTAACAGTAGAAGAAAGGAATCTACTTTCGGTTGGGTACAAAAATG
TGATTGGGGCTCGGCGTGCTTCATGGAGAATAATGTCTAGCATTGAGCAGAAGGAGGAGGCCAAGGGCAATGATCATAATGTGAAGCGTATCAGAGAATATAGACAGAAAGTAGAAGCGGAGCTTTCTAAGATCTGCCATGATATTATGACTATCATTGATGAACACCTCATTCCTTCCTCCAGTATTGGCGAATCTACT
GTTTTTTACTATAAAATGAAAGGAGACTACTACCGTTATCTTGCTGAATTCAAAACAGGAAATGAGAGGAAAGAAGCCGCTGATCAGTCCTTGAAAGCTTACCAGACGGCTTCAAGTACCGCAGAGTCGGATTTACCGCCAACTCATCCAATCAGGCTTGGATTGGCCCTGAACTTCTCTGTTTTCTACTATGAAATAAT
GAATTCACCTGAAAGGGCCTGCCATCTGGCCAAGCAAGCTTTTGACGAAGCTATTGCAGAACTTGACACTTTGAGTGAAGAGTCATACAAAGATAGCACTTTGATCATGCAGCTTTTGAGGGACAACCTCACTTTGTGGACCTCCGATCTTCAGGAAGATGGAGTTGAAGATCAGGCCAAGGGGGATGATCCTGCCGTAG
GCATGGATGAGGAGCTTTAAGCAGGTTTTAGATTGGAACAAAAGACCAGTAGGACATTGTGGGTCTTAACATGGCGTGTATGCCATGGTTATTAAACGCTTCAATATCTATTAGAGGCCCAGCATTGCAGTACTGTGTATGAATTTAGAGTCTTTGGTTGTGCATTGTCATCGTTTTGAAGATTAATATAGGCCTCTGAT
GGATGAAGTGGCTTTGTTGCCACAGAGAAGCTGAATTCTTTGAGCTTTCCTCATTTTCTTTTTCAACTTTTCTCGAAGTTACATGGAATATAGCGGAATAAAAAGGAAATCTTGTATAGATTGCAGGATGTTAGATTAACTTCTCCTGGTATTGAAAAGTGAAGTGTGCAAAGAGATTGACTGTAATTTGATTGCCTTGT
GCCTTAATAGGCATTAGCATTGTTTCAGTTATGAGTTGCAACAATGTTATTTATGTTATGCACAAATATACGTTGCATCGAAGGGAATCCTTAAAATCAAAAA

>TRINITY_DN99444_c0_g2_i2 from sample A4 / Example unspliced isoform:

CTGAGAAGTCTTTTTTTGCCTTGAGATAATAACGGTGGTCTTTTCTATTAAAGTACTTTGTCCAAAGAGTTTTGTGAGAGCAGTCATTTATTGCAATTTACAATGCATTGCTGCAGACGGCTTCAAGTACCGCAGAGTCGGATTTACCGCCAACTCATCCAATCAGGCTTGGATTGGCCCTGAACTTCTCTGTTTTCTAC
TATGAAATAATGAATTCACCTGAAAGGGCCTGCCATCTGGCCAAGCAAGCTTTTGACGAAGCTATTGCAGAACTTGACACTTTGAGTGAAGAGTCATACAAAGATAGCACTTTGATCATGCAGCTTTTGAGGGACAACCTCACTTTGTGGACCTCCGATCTTCAGGAAGATGGAGTTGAAGATCAGGCCAAGGGGGATGA
TCCTGCCGTAGGCATGGATGAGGAGCTTTAAGCAGgtacatgtagaaacaaatgaagttgattagatttatgggcttgtatgttcgcctcgatgtattctagagcaggaatctgattatcctttctgctctgcagctgcaatttacaaattctctctctttatctttgttattcacgcctggttttgtttatagGTTTTA

GATTGGAACAAAAGACCAGTAGGACATTGTGGGTCTTAACATGGCGTGTATGCCATGGTTATTAAACGCTTCAATATCTATTAGAGGCCCAGCATTGCAGTACTGTGTATGAATTTAGAGTCTTTGGTTGTGCATTGTCATCGTTTTGAAGATTAATATAGGCCTCTGATGGATGAAGTGGCTTTGTTGCCACAGAGAAG
CTGAATTCTTTGAGCTTTCCTCATTTTCTTTTTCAACTTTTCTCGAAGTTACATGGAATATAGCGGAATAAAAAGGAAATCTTGTATAGATTGCAGGATGTTAGATTAACTTCTCCTGGTATTGAAAAGTGAAGTGTGCAAAGAGATTGACTGTAATTTGATTGCCTTGTGCCTTAATAGGCATTAGCATTGTTTCAGTT
ATGAGTTGCAACAATGTTATTTATGTTATGCACAAATATACGTTGCATCGAAGGGAATCCTTAAAATCAAAAA