NA ----> NA

Intron 1 GTAAATAATT ... GTGGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.5	5	6	0
A2	42.86	14	18	3
A3	100	0	9	2
A4	55.56	2	4	1
A5	50	2	2	2
A6	46.67	4	7	0
A7	40.54	11	5	10
A8	42.86	6	3	6
A9	45.45	12	15	5
A10	33.33	1	0	1

>TRINITY_DN47033_c1_g6_i1 from sample A7 / Example spliced isoform

CGCGCGCCGGATCATAAGGGAAAAGCCAGTGTTGGTTTTAAAAATAAGTTACACCAAACCGTAGAGGAAGCCGGAAGCCAACTGAAAGATTCAGACTTTTAATAAGGATCACGTGAAGAACAAACAGCGCCAAGCAAGGAGGATTATGCCACGATCTGCCAAGAAATAATCAATATTGGACTCAGCAAATTGCTGCCAAA
TATTATGACAGAAATTACATGATGTTGAGGAAAATGTAGAGTTCGTTGAAAAATATGAAAACAAACTGTTAAAATGAATGAGGATGAAAAATGAAAGTTAACTGAAGTAACGCTAGGCGCCGAGAATCATTGTTCATCGAGAGAAAGGGCACGTTATCACCATTAGCAAAAGAATTGCGTTATTTTTCAATTAATCGAAA
AGCCAAGAAGATAACGAACGAGTGAATAAAAATAGAATCAAAATGAATGAAGACACTCCATTAGCCGTATTTGAAGGATTGAAATCAATACTAACAGATCATTCAGTTTCTTCCACCGATTACTACAGAAAGACATCTGCCCATGGTTTACCAGAACCTTGCGTGAAAGACTGCTACGAAGCTGAAATGATCCGAACTGT
CGAGACTGTATTCGCTCTCCATTGTGGACCTGTCAATTCAAACGCTGAGATGAGCATCAAATCGTTGAGAAGATCCCTGGAATTAACGCATAATCAACATCACCCAGCATAATGTGACCAAGCTACTGCTATGCTACCAGTTCGATGTGAAAGGGAGAATGTTTAACGGTGAATATGTTTTGATGAGATGGAACAGATAT
GAATAAAGGCGCCCAAGAATAGAGGATCATTCAGCGGACTGCAAGCCTTTGAATCGCTGAAATTCTCAAGAGAATTTGCAGATCAAACAGAAGATTGCGGTCATCTTTCCAATAAAATTTCTTCATCGAGAAAGCCATTAAATTGTTGGAAAATCTATCCCGGATTCGCGTGTTTCTTGTTACCCAAAGTTTCTTGTTAG
CAAATTTGTTATAGCTGAAACCTTGTTTTCATTATTCTGTTTCTATTTTGTAAAATTTCTGTTTGCAGAGTGGAGGAGACACTGCCATGGAGGAGGC

>TRINITY_DN47033_c1_g6_i2 from sample A7 / Example unspliced isoform:

CGCGCGCCGGATCATAAGGGAAAAGCCAGTGTTGGTTTTAAAAATAAGTTACACCAAACCGTAGAGGAAGCCGGAAGCCAACTGAAAGATTCAGACTTTTAATAAGGATCACGTGAAGAACAAACAGCGCCAAGCAAGGAGGATTATGCCACGATCTGCCAAGAAATAATCAATATTGGACTCAGCAAATTGCTGCCAAA
TATTATGACAGAAATTACATGATGTTGAGGAAAATGTAGAGTTCGTTGAAAAATATGAAAACAAACTgtaaataattctcaagtcggcgtccgatctatcatacgtcaaaggaatttcatctactttagaatcacactttaaaatgtaaacttgaattaagttaaaatctattttacgatttttacttatttgagttttt
gtatgtggatgcagGTTAAAATGAATGAGGATGAAAAATGAAAGTTAACTGAAGTAACGCTAGGCGCCGAGAATCATTGTTCATCGAGAGAAAGGGCACGTTATCACCATTAGCAAAAGAATTGCGTTATTTTTCAATTAATCGAAAAGCCAAGAAGATAACGAACGAGTGAATAAAAATAGAATCAAAATGAATGAAGA
CACTCCATTAGCCGTATTTGAAGGATTGAAATCAATACTAACAGATCATTCAGTTTCTTCCACCGATTACTACAGAAAGACATCTGCCCATGGTTTACCAGAACCTTGCGTGAAAGACTGCTACGAAGCTGAAATGATCCGAACTGTCGAGACTGTATTCGCTCTCCATTGTGGACCTGTCAATTCAAACGCTGAGATGA
GCATCAAATCGTTGAGAAGATCCCTGGAATTAACGCATAATCAACATCACCCAGCATAATGTGACCAAGCTACTGCTATGCTACCAGTTCGATGTGAAAGGGAGAATGTTTAACGGTGAATATGTTTTGATGAGATGGAACAGATATGAATAAAGGCGCCCAAGAATAGAGGATCATTCAGCGGACTGCAAGCCTTTGAA
TCGCTGAAATTCTCAAGAGAATTTGCAGATCAAACAGAAGATTGCGGTCATCTTTCCAATAAAATTTCTTCATCGAGAAAGCCATTAAATTGTTGGAAAATCTATCCCGGATTCGCGTGTTTCTTGTTACCCAAAGTTTCTTGTTAGCAAATTTGTTATAGCTGAAACCTTGTTTTCATTATTCTGTTTCTATTTTGTAA
AATTTCTGTTTGCAGAGTGGAGGAGACACTGCCATGGAGGAGGC

Intron 2 GTAAATAATT ... TACGTCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.18	5	6	1
A2	39.13	14	16	2
A3	100	0	8	8
A4	33.33	2	2	0
A5	33.33	2	2	0
A6	52.94	4	7	2
A7	29.03	11	5	4
A8	33.33	6	3	3
A9	50	12	15	9
A10	0	1	0	0

>TRINITY_DN47033_c1_g6_i1 from sample A7 / Example spliced isoform

CGCGCGCCGGATCATAAGGGAAAAGCCAGTGTTGGTTTTAAAAATAAGTTACACCAAACCGTAGAGGAAGCCGGAAGCCAACTGAAAGATTCAGACTTTTAATAAGGATCACGTGAAGAACAAACAGCGCCAAGCAAGGAGGATTATGCCACGATCTGCCAAGAAATAATCAATATTGGACTCAGCAAATTGCTGCCAAA
TATTATGACAGAAATTACATGATGTTGAGGAAAATGTAGAGTTCGTTGAAAAATATGAAAACAAACTGTTAAAATGAATGAGGATGAAAAATGAAAGTTAACTGAAGTAACGCTAGGCGCCGAGAATCATTGTTCATCGAGAGAAAGGGCACGTTATCACCATTAGCAAAAGAATTGCGTTATTTTTCAATTAATCGAAA
AGCCAAGAAGATAACGAACGAGTGAATAAAAATAGAATCAAAATGAATGAAGACACTCCATTAGCCGTATTTGAAGGATTGAAATCAATACTAACAGATCATTCAGTTTCTTCCACCGATTACTACAGAAAGACATCTGCCCATGGTTTACCAGAACCTTGCGTGAAAGACTGCTACGAAGCTGAAATGATCCGAACTGT
CGAGACTGTATTCGCTCTCCATTGTGGACCTGTCAATTCAAACGCTGAGATGAGCATCAAATCGTTGAGAAGATCCCTGGAATTAACGCATAATCAACATCACCCAGCATAATGTGACCAAGCTACTGCTATGCTACCAGTTCGATGTGAAAGGGAGAATGTTTAACGGTGAATATGTTTTGATGAGATGGAACAGATAT
GAATAAAGGCGCCCAAGAATAGAGGATCATTCAGCGGACTGCAAGCCTTTGAATCGCTGAAATTCTCAAGAGAATTTGCAGATCAAACAGAAGATTGCGGTCATCTTTCCAATAAAATTTCTTCATCGAGAAAGCCATTAAATTGTTGGAAAATCTATCCCGGATTCGCGTGTTTCTTGTTACCCAAAGTTTCTTGTTAG
CAAATTTGTTATAGCTGAAACCTTGTTTTCATTATTCTGTTTCTATTTTGTAAAATTTCTGTTTGCAGAGTGGAGGAGACACTGCCATGGAGGAGGC

>TRINITY_DN47033_c1_g6_i3 from sample A7 / Example unspliced isoform:

CGCGCGCCGGATCATAAGGGAAAAGCCAGTGTTGGTTTTAAAAATAAGTTACACCAAACCGTAGAGGAAGCCGGAAGCCAACTGAAAGATTCAGACTTTTAATAAGGATCACGTGAAGAACAAACAGCGCCAAGCAAGGAGGATTATGCCACGATCTGCCAAGAAATAATCAATATTGGACTCAGCAAATTGCTGCCAAA
TATTATGACAGAAATTACATGATGTTGAGGAAAATGTAGAGTTCGTTGAAAAATATGAAAACAAACTgtaaataattctcaagtcggcgtccgatctatcatacgtcaaagGTTAAAATGAATGAGGATGAAAAATGAAAGTTAACTGAAGTAACGCTAGGCGCCGAGAATCATTGTTCATCGAGAGAAAGGGCACGTTA
TCACCATTAGCAAAAGAATTGCGTTATTTTTCAATTAATCGAAAAGCCAAGAAGATAACGAACGAGTGAATAAAAATAGAATCAAAATGAATGAAGACACTCCATTAGCCGTATTTGAAGGATTGAAATCAATACTAACAGATCATTCAGTTTCTTCCACCGATTACTACAGAAAGACATCTGCCCATGGTTTACCAGAA
CCTTGCGTGAAAGACTGCTACGAAGCTGAAATGATCCGAACTGTCGAGACTGTATTCGCTCTCCATTGTGGACCTGTCAATTCAAACGCTGAGATGAGCATCAAATCGTTGAGAAGATCCCTGGAATTAACGCATAATCAACATCACCCAGCATAATGTGACCAAGCTACTGCTATGCTACCAGTTCGATGTGAAAGGGA
GAATGTTTAACGGTGAATATGTTTTGATGAGATGGAACAGATATGAATAAAGGCGCCCAAGAATAGAGGATCATTCAGCGGACTGCAAGCCTTTGAATCGCTGAAATTCTCAAGAGAATTTGCAGATCAAACAGAAGATTGCGGTCATCTTTCCAATAAAATTTCTTCATCGAGAAAGCCATTAAATTGTTGGAAAATCT
ATCCCGGATTCGCGTGTTTCTTGTTACCCAAAGTTTCTTGTTAGCAAATTTGTTATAGCTGAAACCTTGTTTTCATTATTCTGTTTCTATTTTGTAAAATTTCTGTTTGCAGAGTGGAGGAGACACTGCCATGGAGGAGGC

Intron 3 GTATAACTTC ... TGTCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.57	6	10	15
A2	86.21	2	17	8
A3	58.97	8	9	14
A4	91.3	1	5	16
A5	57.14	9	13	11
A6	65.06	29	40	68
A7	62.1	83	147	125
A8	64.16	81	155	135
A9	65.96	72	152	127
A10	73.18	35	103	88

>TRINITY_DN44296_c2_g1_i9 from sample A6 / Example spliced isoform

GCCATTTTGTACCAGGTTGGTTGCTCACCCGTTCAACGGAATGCATAAAGGAATTAATAACGAGCAGACTGTTTATAAATAGAAGAATTTGTTTTAACGGAATTATTGAAATAGTGGGCCGGTACTCATCGCATCAATGAAGATAAGCGAGAATCGAAAGGGTTACGCAACACAATACTAAGAAAGTTAGAGGGATCATC
GGAGTTCAGTTATTTAGCATAAATTCACACTATGTGAATTTTGGATAACTAAGATGCCGTATGGATGTATATGCAAGAAGGATAACAGTGACAACTACCTTGTTGAAATTGTAAAACGCCAAGGATTGAAGCAGTTTTAGCTCATCACATGGCGAGAAGAACTAAATGGATTTAATCAAATTGACGGCGCCAGATTATTG
GTGAAAGTGTTCAATATGCAATACGAGAAAGAGAGATTTCACAAAGTTCGCAACGAAGATGAGTAAAAAGATTTTAATAGCTTCTGCCAGGTTTCAAAGTTACCAGATCGCCAGATTGACAAGATTGCTGCCAAGATAAACTGCATCAGCCCATCCAATCAACAAGAACGGAGAATTTTTTGGTGCAGTTGGAGAGGGTA
AGGGTGATCGAACTGTTTGAGAAGGCGAGGGAAGTTCCAGTATTTTTCTGGTTGTTCTATATAAATAGCAGAAGATGTGGACAATGAGAGGGGGGAGGTCTCGACAGAATAAAGAAAATTTGTTTTCTTGATTCTGTTCTCTATTTGTAATCTTTCTGTTTGCAAAAGGAAGAGGAGACACTGCCGTGAGAGAGAACGCT
CAGCATCAATGAAAAGTGTTTCTTTCTTTTTATTTTTAAATTTTGCCTGCAATATTTTTATTGAACACTTGAAGCAGAGGGCCTCTTTTCTCTTCTCATTTTGTCTGGGCTGGTGATCATACTTTGTAATTTTTTATGGAGCTATAATAAAAAGCCAGATAATACTTGCAATCATTTTCTC

>TRINITY_DN44296_c2_g1_i4 from sample A6 / Example unspliced isoform:

GCCATTTTGTACCAGGTTGGTTGCTCACCCGTTCAACGGAATGCATAAAGGAATTAATAACGAGCAGACTGTTTATAAATAGAAGAATTTGTTTTAACGGAATTATTGAAATAGTGGGCCGGTACTCATCGCATCAATGAAGATAAGCGAGAATCGAAAGGGTTACGCAACACAATACTAAGAAAGTTAGAGGGATCATC
GGAGTTCAGTTATTTAGCATAAATTCACACTATGTGAATTTTGGATAACTAAGATGCCGTATGGATGTATATGCAAGAAGGATAACAGTGACAACTACCTTGTTGAAATTGTAAAACGCCAAGGATTGAAGCAGTTTTAGCTCATCACATGGCGAGAAGAACTAAATGGATTTAATCAAATTGACGGCGCCAGATTgtat
aacttcgtaacattgcactatatcaagacataatgatatcaattctaggggagatctctatacttttctttactttcatgataattctttataaacaaaattctgccatttcgttttaggttaatgaatacttttgtcttgcagATTGGTGAAAGTGTTCAATATGCAATACGAGAAAGAGAGATTTCACAAAGTTCGCA
ACGAAGATGAGTAAAAAGATTTTAATAGCTTCTGCCAGGTTTCAAAGTTACCAGATCGCCAGATTGACAAGATTGCTGCCAAGATAAACTGCATCAGCCCATCCAATCAACAAGAACGGAGAATTTTTTGGTGCAGTTGGAGAGGGTAAGGGTGATCGAACTGTTTGAGAAGGTGAGGGAAGTTCTCTTGGATGGTCTAT
ATAAGTAGTAGCAAGTCGACTACCGTGAAGGGGGGGTCTCGTCAGAATAAGTGAAACCTTGTTTTCATTATTCTGTTTCTATTTTGTAAAATTTCTGTTTGCAGGGTGGAGGAGATACTGCCATGGAGTAGGCTCTGTAAAGGTTGAAGGGAACTCTTTCCCAGAAGCTTTATTATTTTCTCCTATTGGTTCGAATTCTG
GATAATAGAGGAAGACGATATCAAAACAGCATGTAATAATGTTATTTTCTGCTTAAATTTGAGTTTTTATGAACTATTTCTGAAGACTGGACTTATTGCATAAAGCTGAGACTCTAATCAAGTTTCTGTTACATATATTATTCATGCTGTATGTTAAGTCATCATATAATGAATTTGGATTAGCATGTTTATTTTGGAAT
CTGGGTGATTTACCTCAAAGCTGGAGTGACA