sp|Q94B38|GPT2_ARATH ----> Glucose-6-phosphate/phosphate translocator 2, chloroplastic

Intron 1 GTTGGTCCTC ... GACTCTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.14	12	4	9
A2	17.65	14	1	5
A3	30.77	18	7	9
A4	24.32	14	4	5
A5	11.11	4	1	0
A6	24.41	96	20	42
A7	33.55	209	98	113
A8	36.77	135	79	78
A9	35.22	218	133	104
A10	36.04	205	110	121

>TRINITY_DN113740_c3_g1_i4 from sample A3 / Example spliced isoform

TCTCTTCATGGCTCATATGCATCGATCTCTTCATTTCATTTCTCAATGGCTCATATGTAATATGTGAAGTCTCACAGGATTTATGGGTGCCATGATATCTAACCTGGCATGTGTGTTCTAGAACATACTCTCCAAGAAAGGAATGGAGGGGAAAGGGAAGTCAGTGGGCGGCATGAACTACTCTGCATGACACCGTTTTC
GTTTGATGTGGGCTGCTGGATGGCAGAACGCTATGAACGCCGTTGGTAGTTGGTCAGAGTGTGTTCAAAATGAAATATCGCCATTAACATTCAGCAAAGGCAACACAATGAAAAGGGTTTTCACTATTGTTTCATCTATCATTATCTTCCACACACCAGTAATCCCCATTAGCGCTATCGGAGCGACGGTTGCCATTTTG
AGTACATTCCTATATTCGCAGGCAAAGGAATGACGAACATACAGAAAGACAATGCTCTTTTCAAAATGAGATGGAAGAACATCCAAGTCCCTGTCAGCTCATTTTCATGTTCGACTCGATTGTGAGACTGTCACGCCATTTTCATTTTCATGTTGGACTTGATTGTGTTTCAGATTTGCGTGTTGAAGAGCGGCGGTGTG
TTTTTGTTTGTGATGTGTTTACCTACGTCCTGTGTTTTTCTGTTTGGTGTTTGTTGTAATTTATGTATTTTATTTTCTGTTGTAGTTGTGATTATTGAGAGAGCCGAGGAGATCACGAGAGGAACACATTTAACGCTATGTATTATGAAGCTCTGCACAAGTAATATCAGTGAGGACGATGACGACATGCTTCTCCAAGA
TCTTTTACTCCATCACCTGCTTCCACTGCGATCGTGTTTGGAGACTAGGGTCGAACTTACCAG

>TRINITY_DN113740_c3_g1_i2 from sample A3 / Example unspliced isoform:

GAAGTCTCACAGGTACTGTAAATTCTCGGTACCTCGGTGCCTCCTAGGAGAACATTTTCCACTGCGTGTCTACTTGTCTTTGCTGCGTATCATAGGGGTCTGCTCTATTTCAATATTACAGGATTTATGGGTGCCATGATATCTAACCTGGCATGTGTGTTCTAGAACATACTCTCCAAGAAAGGAATGGAGGGGAAAGG
GAAGTCAGTGGGCGGCATGAACTACTCTGCATGACACCGTTTTCGTTTGATGTGGGCTGCTGGATGGCAGAACGCTATGAACGCCgttggtcctcaatttgcatgatattttcactgctatgttgttctttttagacattatatatataataataatactgtgacaattattctaaccttcaataattgactctccagGT
TGGTAGTTGGTCAGAGTGTGTTCAAAATGAAATATCGCCATTAACATTCAGCAAAGGCAACACAATGAAAAGGGTTTTCACTATTGTTTCATCTATCATTATCTTCCACACACCAGTAATCCCCATTAGCGCTATCGGAGCGACGGTTGCCATTTTGAGTACATTCCTATATTCGCAGGCAAAGGAATGACGAACATACA
GAAAGACAATGCTCTTTTCAAAATGAGATGGAAGAACATCCAAGTCCCTGTCAGCTCATTTTCATGTTCGACTCGATTGTGAGACTGTCACGCCATTTTCATTTTCATGTTGGACTTGATTGTGTTTCAGATTTGCGTGTTGAAGAGCGGCGGTGTGTTTTTGTTTGTGATGTGTTTACCTACGTCCTGTGTTTTTCTGT
TTGGTGTTTGTTGTAATTTATGTATTTTATTTTCTGTTGTAGTTGTGATTATTGAGAGAGCCGAGGAGATCACGAGAGGAACACATTTAACGCTATGTATTATGAAGCTCTGCACAAGTAATATCAGTGAGGACGATGACGACATGCTTCTCCAAGATCTTTTACTCCATCACCTGCTTCCACTGCGATCGTGTTTGGAG
ACTAGGGTCGAACTTACCAG

Intron 2 GTGAGACTGT ... GATTACTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	2	2
A2	60	3	5	4
A3	75	3	6	12
A4	100	0	0	5
A5	83.33	1	2	8
A6	68.37	31	39	95
A7	79.18	38	119	170
A8	71.61	68	130	213
A9	64.49	87	123	193
A10	81.88	28	94	159

>TRINITY_DN44136_c2_g2_i4 from sample A9 / Example spliced isoform

TGGTAGTTGGTCAGAGTGTGTTCAAAATGAAATATCGCCATTAACATTCAGCAAAGGCAACACAATGAAAAGGGTTTTCACTATTGTTTCATCTATCATTATCTTCCACACACCAGTAATCCCCATTAGCGCTATCGGAGCGACGGTTGCCATTTTGAGTACATTCCTATATTCGCAGGCAAAGGAATGACGAACATACA
GAAAGACAATGCTCTTTTCAAAATGAGATGGAAGAACATCCAAGTCCCTGTCAGTTCATTTTCATGTTCGACTCGATTAGAGGAGAGGAGGCCGTGCGAGGAACATGTTTAGAGCACGAGAGAGGAGAGGAGGATTTTCCGCTACGATCTGTGTCAAACTTAGG

>TRINITY_DN44136_c2_g2_i1 from sample A9 / Example unspliced isoform:

CAAAGGAATGACGAACATACAGAAAGACAATGCTCTTTTCAAAATGAGATGGAAGAACATCCAAGTCCCTGTCAGCTCATTTTCATGTTCGACTCGATTgtgagactgtcacgccattttcattttcatgttggacttgattgtgtttcagatttgcgtgttgaagagcggcggtgtgtttttgtttgtgatgtgtttac
ctacgtcctgtgtttttctgtttggtgtttgttgtaatttatgtatcttattttctgttgtatttgtgattactgagAGAGGAGAGGAGGCCGTGCGAGGAACATGTTTAGAGCACGAGAGAGGAGAGGAGGATTTTCCGCTACGATCTGTGTCAAACTTAGG

Intron 3 GTATAATATT ... TCATTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.64	19	2	4
A2	25.71	13	6	3
A3	21.74	9	2	3
A4	33.33	5	3	2
A5	11.11	4	1	0
A6	21.21	65	18	17
A7	23.55	112	30	39
A8	20.7	159	42	41
A9	22.42	173	45	55
A10	20.85	112	37	22

>TRINITY_DN44136_c2_g2_i4 from sample A9 / Example spliced isoform

TGGTAGTTGGTCAGAGTGTGTTCAAAATGAAATATCGCCATTAACATTCAGCAAAGGCAACACAATGAAAAGGGTTTTCACTATTGTTTCATCTATCATTATCTTCCACACACCAGTAATCCCCATTAGCGCTATCGGAGCGACGGTTGCCATTTTGAGTACATTCCTATATTCGCAGGCAAAGGAATGACGAACATACA
GAAAGACAATGCTCTTTTCAAAATGAGATGGAAGAACATCCAAGTCCCTGTCAGTTCATTTTCATGTTCGACTCGATTAGAGGAGAGGAGGCCGTGCGAGGAACATGTTTAGAGCACGAGAGAGGAGAGGAGGATTTTCCGCTACGATCTGTGTCAAACTTAGG

>TRINITY_DN44136_c2_g2_i3 from sample A9 / Example unspliced isoform:

TGGTAGTTGGTCAGAGTGTGTTCAAAATGAAATATCGCCATTAACATTCAGCAAAGGCAACACAATGAAAAGGGTTTTCACTATTGTTTCATCTATCATTATCTTCCACACACCAGTAATCCCCATTAGCGCTATCGGAGCGACGGTTGCCATTTTGAGTACATTCCTATATTCGCAGgtataatatttgtatttgtaat
gcaaatgttcatgtgaagagatgaaaatatatctcggatgatggtacctattgacttggatttcatttgcagGCAAAGGAATGACGAACATACAGAAAGACAATGCTCTTTTCAAAATGAGATGGAAGAACATCCAAGTCCCTGTCAGCTCATTTTCATGTTCGACTCGATTGTGAGACTGGTACGTCATTTTTATTTTC
ATGTTGGACTTGATTGTCTTTCAGATTTGCGTGTTGAAGAGCCGCGGTGTGTTTTTGTTTGTGATGTGTTTACCTACGTGCTGTGTTTTTGTGTGTGATGCGCTTTTGTGTTTGGTGG