NA ----> NA

Intron 1 GTAGGGTTTT ... AAATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.13	140	37	25
A2	16.77	134	30	24
A3	16.4	130	37	14
A4	19.7	108	36	17
A5	13.82	53	13	4
A6	15.82	173	40	25
A7	16.2	313	89	32
A8	14.88	183	32	32
A9	16.01	307	86	31
A10	15.75	321	84	36

>TRINITY_DN121370_c0_g1_i5 from sample A1 / Example spliced isoform

CACACTTTGAAACTCCCATCGAGTCTTAGCTGTTGTCTCTGAGGACTCTTTGGTCTTTTCTCTAATCATTATTCCGGCACCAGCAGTAGCAGAGGGAGCCTTGAGCTGGGAATTCATTTGACGGCCATGCCATGATCGAAGAAGATCTGTTTTGGTGTTGGGATTCAGGCTTTTCTGCAAATAACATCATCTCGATCTCA
ATCTCAATCATCACGTGGAGGATTCTTAGCAGCCCGAGTCTTTTACCGGAGGGCTAGGGTATGACTCCGTCTGGCACAGTGGCTCTTTCAAAGGGAATCGTCAAGGAGCGAGAGATGGCTGCTAGCGTCTCCAGTGTTTACATACACGTGGTTGATAGTGTTATGAAAACTCTCCGGCCAGAATTTCAGAATGAGGGTTT
AGATGACAGTGTCTTAAATGAAGTCCAAGCGCTTTGGGAATTAAAAATGATGCAAAATGGGGCAATACAAGGTCCAATAGACCATGCATCTAATTCTGCTCTACGAGGCGCAAATGCTCCTATCACCCCTGTTCATGATCTCAATGTACCTTATGAACCAACTGAAGAATATGAGACTCCTACTGCGGAAATGCTTTTCC
CACCAACACCTATACAGACACCTATGCAGACTCCTTTGCCAGGTACTACAGAGCCAGTTATGTACCAATACTTTCCACCTGGTCCAGGTGAGGCTCTGGTGATTCCCGAGTCAGGGATTGACCCAGAAGCGAAGATTGGAAGGCCGGCTTCTTACATGCAACAACCCGCAAACTGGATGAGTCATAAGCCCCTTGGAGTG
GATGTCAATGTTGGTGAGTATTGTATTAAAAATATTCACTGGTGTACCAGGATATGAAATTGAAAGTTATTTGTTGAATTGTTTTCATGGGAAATATCTAGACAGTGAGTATAAACAAATAAGATGACTATTACCTTGGTGAACAATACAGGATGTTTCTTGAATCTTCTGACTTTTCTGTAATGTTGCAGCTTATGAGG
AAGAGAGGGATGA

>TRINITY_DN121370_c0_g1_i1 from sample A1 / Example unspliced isoform:

CACACTTTGAAACTCCCATCGAGTCTTAGCTGTTGTCTCTGAGGACTCTTTGGTCTTTTCTCTAATCATTATTCCGGCACCAGCAGTAGCAGAGGGAGCCTTGAGCTGGGAATTCATTTGACGGCCATGCCATGATCGAAGAAGATCTGTTTTGGTGTTGGGATTCAGGCTTTTCTGCAAATAACATCATCTCGATCTCA
ATCTCAATCATCACgtagggtttttgttgccgaaaaaatcagctggactactgaatcacgttttttcattaatttcaaacccctcccccacatactattatcatgctccagattttgtcaatacagtgccattgtggtgatccatttacatggaatctgacttaattcagtagaacttgatgttactcgatatagcagtc
tctataaatcgggatttgatggctttagataaaagattcgtgctttctttttgtatttgaagaaaatgttgttgtggaaattttcagGTGGAGGATTCTTAGCAGCCCGAGTCTTTTACCGGAGGGCTAGGGTATGACTCCGTCTGGCACAGTGGCTCTTTCAAAGGGAATCGTCAAGGAGCGAGAGATGGCTGCTAGCG
TCTCCAGTGTTTACATACACGTGGTTGATAGTGTTATGAAAACTCTCCGGCCAGAATTTCAGAATGAGGGTTTAGATGACAGTGTCTTAAATGAAGTCCAAGCGCTTTGGGAATTAAAAATGATGCAAAATGGGGCAATACAAGGTCCAATAGACCATGCATCTAATTCTGCTCTACGAGGCGCAAATGCTCCTATCACC
CCTGTTCATGATCTCAATGTACCTTATGAACCAACTGAAGAATATGAGACTCCTACTGCGGAAATGCTTTTCCCACCAACACCTATACAGACACCTATGCAGACTCCTTTGCCAGGTACTACAGAGCCAGTTATGTACCAATACTTTCCACCTGGTCCAGGTGAGGCTCTGGTGATTCCCGAGTCAGGGATTGACCCAGA
AGCGAAGATTGGAAGGCCGGCTTCTTACATGCAACAACCCGCAAACTGGATGAGTCATAAGCCCCTTGGAGTGGATGTCAATGTTG

Intron 2 GTGAGTATTG ... AATGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.53	80	17	0
A2	7.59	73	6	4
A3	18.63	83	19	1
A4	17.89	78	17	0
A5	17.39	38	8	1
A6	13.39	110	17	0
A7	10.51	281	33	0
A8	12.4	226	32	0
A9	16.22	248	48	0
A10	17.45	227	48	0

>TRINITY_DN121370_c0_g1_i4 from sample A1 / Example spliced isoform

CACACTTTGAAACTCCCATCGAGTCTTAGCTGTTGTCTCTGAGGACTCTTTGGTCTTTTCTCTAATCATTATTCCGGCACCAGCAGTAGCAGAGGGAGCCTTGAGCTGGGAATTCATTTGACGGCCATGCCATGATCGAAGAAGATCTGTTTTGGTGTTGGGATTCAGGCTTTTCTGCAAATAACATCATCTCGATCTCA
ATCTCAATCATCACGTGGAGGATTCTTAGCAGCCCGAGTCTTTTACCGGAGGGCTAGGGTATGACTCCGTCTGGCACAGTGGCTCTTTCAAAGGGAATCGTCAAGGAGCGAGAGATGGCTGCTAGCGTCTCCAGTGTTTACATACACGTGGTTGATAGTGTTATGAAAACTCTCCGGCCAGAATTTCAGAATGAGGGTTT
AGATGACAGTGTCTTAAATGAAGTCCAAGCGCTTTGGGAATTAAAAATGATGCAAAATGGGGCAATACAAGGTCCAATAGACCATGCATCTAATTCTGCTCTACGAGGCGCAAATGCTCCTATCACCCCTGTTCATGATCTCAATGTACCTTATGAACCAACTGAAGAATATGAGACTCCTACTGCGGAAATGCTTTTCC
CACCAACACCTATACAGACACCTATGCAGACTCCTTTGCCAGGTACTACAGAGCCAGTTATGTACCAATACTTTCCACCTGGTCCAGGTGAGGCTCTGGTGATTCCCGAGTCAGGGATTGACCCAGAAGCGAAGATTGGAAGGCCGGCTTCTTACATGCAACAACCCGCAAACTGGATGAGTCATAAGCCCCTTGGAGTG
GATGTCAATGTTGCTTATGAGGAAGAGAGGGATGATATGGCAGCTGGAATTAGTCAAGGACCTGTCACAAAGGATTTTTTCACTTTGAGTAGTGGCAAGCGAAAACGGGAAGACCACCCTTCAAATTATCTTCCAGGGGGCTACATTCCACAACAAGATGGAGCAGGGGATTTTTCTCGGGAGTTAGTCCAATCACAGGA
GGTGGAATTTACAAACCAAAGGCTTGCACAAAATTACTCAACAGATGGGTCAATGTTTGAAAAGAGGCTGAAGTCCGATCCTCTAATTACTGCTCTTGTTAAAGCCAAGTATCGATCTCTGGAAATTCCCCAGGTTGATGGTGTTAATGACAATTTAGACAGTACGGTTGCGGACGAGGGCAACATTGAAGCAAAAGATC
AGGAGCAACACGCTTTAAACGATGTTGCCTCTTCGAAAGTGTCAGAGGATGAGGAGCCTTTAAATGATAATGATGATGATCCAGATGACTTGGATCAGACAAGCGAGGACCCAAAAACAGATGACCTAGTTCTTGCTCAGTTTGAGAAGGTGTCACGGACCAAGAATAGATGGAAATGTATTCTAAAGGATGGCGTAATG
CACCTAAACAATAAAGATATTCTTTTCTCGAAGGTATATATGTCAAGAACTCATGGTCGGATCTATGCTTGTGCTTGGGTTTCTGTGCTATTCTAATAACTTCTATTTTAACTTGTGATGTTTCTGCAG

>TRINITY_DN121370_c0_g1_i5 from sample A1 / Example unspliced isoform:

CACACTTTGAAACTCCCATCGAGTCTTAGCTGTTGTCTCTGAGGACTCTTTGGTCTTTTCTCTAATCATTATTCCGGCACCAGCAGTAGCAGAGGGAGCCTTGAGCTGGGAATTCATTTGACGGCCATGCCATGATCGAAGAAGATCTGTTTTGGTGTTGGGATTCAGGCTTTTCTGCAAATAACATCATCTCGATCTCA
ATCTCAATCATCACGTGGAGGATTCTTAGCAGCCCGAGTCTTTTACCGGAGGGCTAGGGTATGACTCCGTCTGGCACAGTGGCTCTTTCAAAGGGAATCGTCAAGGAGCGAGAGATGGCTGCTAGCGTCTCCAGTGTTTACATACACGTGGTTGATAGTGTTATGAAAACTCTCCGGCCAGAATTTCAGAATGAGGGTTT
AGATGACAGTGTCTTAAATGAAGTCCAAGCGCTTTGGGAATTAAAAATGATGCAAAATGGGGCAATACAAGGTCCAATAGACCATGCATCTAATTCTGCTCTACGAGGCGCAAATGCTCCTATCACCCCTGTTCATGATCTCAATGTACCTTATGAACCAACTGAAGAATATGAGACTCCTACTGCGGAAATGCTTTTCC
CACCAACACCTATACAGACACCTATGCAGACTCCTTTGCCAGGTACTACAGAGCCAGTTATGTACCAATACTTTCCACCTGGTCCAGGTGAGGCTCTGGTGATTCCCGAGTCAGGGATTGACCCAGAAGCGAAGATTGGAAGGCCGGCTTCTTACATGCAACAACCCGCAAACTGGATGAGTCATAAGCCCCTTGGAGTG
GATGTCAATGTTGgtgagtattgtattaaaaatattcactggtgtaccaggatatgaaattgaaagttatttgttgaattgttttcatgggaaatatctagacagtgagtataaacaaataagatgactattaccttggtgaacaatacaggatgtttcttgaatcttctgacttttctgtaatgttgcagCTTATGAGG
AAGAGAGGGATGA

Intron 3 GTTGATCTCC ... ATATTGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75	18	81	27
A2	68.42	15	40	25
A3	75.93	13	57	25
A4	79.49	8	57	5
A5	75.44	7	31	12
A6	72.39	78	321	88
A7	73.58	93	390	128
A8	78.48	130	676	272
A9	80.65	65	381	161
A10	74.45	70	294	114

>TRINITY_DN102166_c0_g1_i2 from sample A5 / Example spliced isoform

CTCCCATCGAGTCTTAGCTGTTGTCTCTGAGGACTCTTTGGTCTTTTCTCTAATCATTATTCCGGCACCAGCAGTAGCAGAGGGAGCCTTGAGCTGGGAATTCATTTGACGGCCATGCCATGATCGAAGAAGATCTGTTTTGGTGTTGGGATTCAGGCTTTTCTGCAAATAACATCATCTCGATCTCAATCTCAATCATC
ACGTGGAGGATTCTTAGCAGCCCGAGTCTTTTACCGGAGGGCTAGGGTATGACTCCGTCTGGCACAGTGGCTCTTTCAAAGGGAATCGTCAAGGAGCGAGAGATGGCTGCTAGCGTCTCCAGTGTTTACATACACGTGGTTGATAGTGTTATGAAAACTCTCCGGCCAGAATTTCAGAATGAGGGTTTAGATGACAGTGT
CTTAAATGAAGTCCAAGCGCTTTGGGAATTAAAAATGATGCAAAATGGGGCAATACAAGGTCCAATAGACCATGCATCTAATTCTGCTCTACGAGGCGCAAATGCTCCTATCACCCCTGTTCATGATCTCAATGTACCTTATGAACCAACTGAAGAATATGAGACTCCTACTGCGGAAATGCTTTTCCCACCAACACCTA
TACAGACACCTATGCAGACTCCTTTGCCAGGTACTACAGAGCCAGTTATGTACCAATACTTTCCACCTGGTCCAGGTGAGGCTCTGGTGATTCCCGAGTCAGGGATTGACCCAGAAGCGAAGATTGGAAGGCCGGCTTCTTACATGCAACAACCCGCAAACTGGATGAGTCATAAGCCCCTTGGAGTGGATGTCAATGTT
GCTTATGAGGAAGAGAGGGATGATATGGCAGCTGGAATTAGTCAAGGACCTGTCACAAAGGATTTTTTCACTTTGAGTAGTGGCAAGCGAAAACGGGAAGACCACCCTTCAAATTATCTTCCAGGGGGCTACATTCCACAACAAGATGGAGCAGGGGATTTTTCTCGGGAGTTAGTCCAATCACAGGAGGTGGAATTTAC
AAACCAAAGGCTTGCACAAAATTACTCAACAGATGGGTCAATGTTTGAAAAGAGGCTGAAGTCCGATCCTCTAATTACTGCTCTTGTTAAAGCCAAGTATCGATCTCTGGAAATTCCCCAGGTTGATGGTGTTAATGACAATTTAGACAGTACGGTTGCGGACGAGGGCAACATTGAAGCAAAAGATCAGGAGCAACACG
CTTTAAACGATGTTGCCTCTTCGAAAGTGTCAGAGGATGAGGAGCCTTTAAATGATAATGATGATGATCCAGATGACTTGGATCAGACAAGCGAGGACCCAAAAACAGATGACCTAGTTCTTGCTCAGTTTGAGAAGGTGTCACGGACCAAGAATAGATGGAAATGTATTCTAAAGGATGGCGTAATGCACCTAAACAAT
AAAGATATTCTTTTCTCGAAGGCAACTGGGGAATTTGAATTTTAAGATTACCTCTTGATTACATGCGAGTCCACCGGATTATGTCCATGACTCCATTCAGCTATCTATTAGGGCACTGATCAAATTGAAAGTGCAAAAGCTGTCATCACATTGCATGAAACAGGAGGGTATATTTTGTAATAACTTTTAAGTATTAGAG

>TRINITY_DN102166_c0_g1_i3 from sample A5 / Example unspliced isoform:

CTCCCATCGAGTCTTAGCTGTTGTCTCTGAGGACTCTTTGGTCTTTTCTCTAATCATTATTCCGGCACCAGCAGTAGCAGAGGGAGCCTTGAGCTGGGAATTCATTTGACGGCCATGCCATGATCGAAGAAGATCTGTTTTGGTGTTGGGATTCAGGCTTTTCTGCAAATAACATCATCTCGATCTCAATCTCAATCATC
ACGTGGAGGATTCTTAGCAGCCCGAGTCTTTTACCGGAGGGCTAGGGTATGACTCCGTCTGGCACAGTGGCTCTTTCAAAGGGAATCGTCAAGGAGCGAGAGATGGCTGCTAGCGTCTCCAGTGTTTACATACACGTGGTTGATAGTGTTATGAAAACTCTCCGGCCAGAATTTCAGAATGAGGGTTTAGATGACAGTGT
CTTAAATGAAGTCCAAGCGCTTTGGGAATTAAAAATGATGCAAAATGGGGCAATACAAGGTCCAATAGACCATGCATCTAATTCTGCTCTACGAGGCGCAAATGCTCCTATCACCCCTGTTCATGATCTCAATGTACCTTATGAACCAACTGAAGAATATGAGACTCCTACTGCGGAAATGCTTTTCCCACCAACACCTA
TACAGACACCTATGCAGACTCCTTTGCCAGGTACTACAGAGCCAGTTATGTACCAATACTTTCCACCTGGTCCAGGTGAGGCTCTGGTGATTCCCGAGTCAGGGATTGACCCAGAAGCGAAGATTGGAAGGCCGGCTTCTTACATGCAACAACCCGCAAACTGGATGAGTCATAAGCCCCTTGGAGTGGATGTCAATGTT
GCTTATGAGGAAGAGAGGGATGATATGGCAGCTGGAATTAGTCAAGGACCTGTCACAAAGGATTTTTTCACTTTGAGTAGTGGCAAGCGAAAACGGGAAGACCACCCTTCAAATTATCTTCCAGGGGGCTACATTCCACAACAAGATGGAGCAGGGGATTTTTCTCGGGAGTTAGTCCAATCACAGGAGGTGGAATTTAC
AAACCAAAGGCTTGCACAAAATTACTCAACAGATGGGTCAATGTTTGAAAAGAGGCTGAAGTCCGATCCTCTAATTACTGCTCTTGTTAAAGCCAAGTATCGATCTCTGGAAATTCCCCAGGTTGATGGTGTTAATGACAATTTAGACAGTACGGTTGCGGACGAGGGCAACATTGAAGCAAAAGATCAGGAGCAACACG
CTTTAAACGATGTTGCCTCTTCGAAAGTGTCAGAGGATGAGGAGCCTTTAAATGATAATGATGATGATCCAGATGACTTGGATCAGACAAGCGAGGACCCAAAAACAGATGACCTAGTTCTTGCTCAGTTTGAGAAGGTGTCACGGACCAAGAATAGATGGAAATGTATTCTAAAGGATGGCGTAATGCACCTAAACAAT
AAAGATATTCTTTTCTCGAAGGCAACTGGGGAATTTGAATTTTAAGATTACCTCTTGATTACATGTGAATCCACCGGATTATGTCCATTCAGCTATCTATTAGGgttgatctccaacccattagtttcgaccacattatcacttggatgtactttttttagaaccacatattctatagttcttactttggctgtaatgat
tgctgaaattaattgatttaaagcgtcttctcttttttgtttcatttccaagtctattttcatgagatgtgaggcagattgcagctctagaaagttttttctttgttttgatatttttacttaattagcttcccttggatattgcaagGCACTGATCAAATTGAAAGTGCAAAAGCTGTCATCACATTGCATGAAACAGG
AGGGTATATTTTGTAATAACTTTTAAGTATTAGAACCCAGATACTGATCAGTATCATTACTGCTGAAATTGCAATATTAAAAATTGCATTACAGCCCAAATTTTTATCCTGAACTACATGGAATATTGACAAGCTCTACAGCATAAATGAGAAGCAGATCAGAATGTGTCTGAACACTGAAATGGTCTTCTTCATGTATA
TATTTTTATCCTTATTCATCATATTATATGCTCATTCTTTT