sp|F4KB17|KTN83_ARATH ----> Katanin p80 WD40 repeat-containing subunit B1 homolog KTN80.3

Intron 1 GTTATGGACT ... GTTAGTGCAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	2	0
A2	100	0	2	0
A3	-1	0	0	0
A4	100	0	0	1
A5	-1	0	0	0
A6	100	0	6	0
A7	77.78	1	5	2
A8	74.16	2	4.5	7
A9	51.92	3	3.5	3
A10	100	0	3	1

>TRINITY_DN44293_c3_g2_i2 from sample A8 / Example spliced isoform

CAATGGTGGAGTCTGGTCGTGCTCGTACTCTAGTTGCAAGTTGGGAAACATGAGAAAAATCTCTCACTGCAGACAAACCTATATTGGTCAGTGGTCGTCCCACCACAAAGAGGAAGCCAAGGGACTTCTGAAAGTGATTGGACAGTTAGTTATGATCAAAGTTCTATAGAAGACCTCATGCAACAACACATGTTTACAAG
CATTCTTCAAACCTGCTCGACTAAGTTGCACGTAATAATGAGGGACAATTTTATAACTTATCTTGAGATCCATTTGCATGAAAATGTACAACCTATTTAAGAAAATGGTATTCTCCTGGTCATTATGATACACATTAAATTATATTCTATGCCAGCACTAGGGATATGCGAATATTTCAGGTCACATATGGATAGGTTTT
CAAATTTCATTATATTTATGATGAACTCTAAAATTGTATTTTTAATTGTACAACTTGGGCTAGGGATATGGGAAGATTTCATGTGCATTTGGATAGGTT

>TRINITY_DN44293_c3_g2_i4 from sample A8 / Example unspliced isoform:

CAAAGATCTTATTTTAATTTAGAAATTGTGCTTGAGCATTATTTTTTCTTTTTAAACCGCATGGCAGATGATGGAGTTGTATGTCAGCCAGATCAAGAAGTTACTGCGTCTAAATTATCAAAATGAGGTATTTAGAAGTTGGCTTCATTGAATTTCGTTAATTTTTTTCTCTTGATGATAGTGTAGCTTTGTTCAAATTT
GTTCAGCATATTGTATTGTAAGTATAGTATTTTGAAAGATTGAGATATATATTTTGCATGTATTAGCAATGGTGGAGTCTGGTCGTGCTCGTACTCTAGTTGCAAGTTGGGAAACATGAGAAAAATCTCTCACTGCAGACAAACCTATATTGGTCAGTGGTCGTCCCACCACAAgttatggactagacataagctcattc
atgtcggtatggaatttttagttcctcacaagttggaaagcattcatttaaatgacaaaatttcttcaaggcgactgtccacactcttttggaagtgtgcttgtttaaattgttcttctattccagatttggcatttggctttcctaaaatatcttttttctttttccaccattgcttaacattggcgatcatatatggt
tagtgcagAGAGGAAGCCAAGGGACTTCTGAAAGTGATTGGACAGTTAGTTATGATCAAAGTTCTATAGAAGACCTCATGCAACAACACATGTTTACAAGCATTCTTCAAACCTGCTCGACTAAGTTGCACGTAATAATGAGGGACAATTTTATAACTTATCTTGAGATCCATTTGCATGAAAATGTACAACCTATTTAA
GAAAATGGTATTCTCCTGGTCATTATGATACACATTAAATTATATTCTATGCCAGCACTAGGGATATGCGAATATTTCAGGTCACATATGGATAGGTTTTCAAATTTCATTATATTTATGATGAACTCTAAAATTGTATTTTTAATTGTACAACTTGGGCTAGGGATATGGGAAGATTTCATGTGCATTTGGATAGGTT

Intron 2 GTATGGAATT ... GTTAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	53	0	0
A2	2.94	33	2	0
A3	0	51	0	0
A4	1.64	30	0	1
A5	0	12	0	0
A6	3.7	39	3	0
A7	5.15	61.3	5	1.7
A8	8.53	59	4	7
A9	3.97	60	3	2
A10	3.37	43	2	1

>TRINITY_DN47149_c0_g1_i5 from sample A7 / Example spliced isoform

GGAAGGGCTTTGCAGAAACAGATTCCAAGTTTGCAACATTGGCTCCTTACATGTGTGGTGCTCGTTTGCTCAGATAGGATCTGCTTGAGTGCCTTTTTTAGTTTGGTTTCTCTTCAAATAATAATATTCATAGGATTACACAGATGGTGGGCTTTTTGTCTGGATTTGGACCTTGTTTGGGGACCGTGGAGGGGATTGAG
TTTCATGCTTTTCCTTCTTTGAAAGACTTGTCTTTGTTCGCTGAAGGGTAGCTTCTGAAGGTAGGATTCGGTTGCAGGTTTGATTCTCTCCCTTCTAGTTATTTATTTATTTTTAAAATGTAATTTAATATTTCCTCCTTGGGAGGTGCATCAAGAAATCCGAAATAGAGTTCGAGCTGCTCTATTGCAAATAACGGACT
TCATCTCTTTGAAACTGATGCCATAACAATGAGAGCATACCACAACTTGGACAGTTTAATACCTCGTCTTGCTCAGACCTTCTAATGAAGGCATGGTCATGGGTCAATCCAGCTAGCTTTGCCAACCAGCAACTTTCAACTATCTCCTTATTGCATTCATGCATTAGTAGTGTTTTTCATGTGAAGGCATTTTGGGGTAT
TTCTCATTTGGTTCACATTCAAAGATCTTATTTTAATTTAGAAATTGTGCTTGAGCATTATTTTTTCTTTTTAAACCGCATGGCAGATGATGGAGTTGTATGTCAGCCAGATCAAGAAGTTACTGCGTCTAAATTATCAAAATGAGGTATTTAGAAGTTGGCTTCATTGATTTTCGTTAATTTTTTTCTCTTGATGATAG
TGTAGCTTTGTTCAAATTTGTTCAGCATATTGTATTGTAAGTATAGTATTTTGAAAGATTGAGATATATATTTTGCATGTATTAGCAATGGTGGAGTCTGGTCGTGCTCGTACTCTAGTTGCAAGTTGGGAAACATGAGAAAAATCTCTCACTGCAGACAAACCTATATTGGTCAGTGGTCGTCCCACCACAAGTTATGG
ACTAGACATAAGCTCATTCATGTCGAGAGGAAGCCAAGGGACTTCTGAAAGTGATTGGACAGTTAGTTATGATCAAAGTTCTATAGAAGACCTCATGCAACAACAC

>TRINITY_DN47149_c0_g1_i11 from sample A7 / Example unspliced isoform:

ATCACGGGGTCTATGCGGGTGCCGTCCCTGCCTGGTGTTCCGACTACATATGGCTCTGCCACTGTTGATGATGGAGTTGTATGTCAGCCAGATCAAGAAGTTACTGCGTCTAAATTATCAAAATGAGCAATGGTGGAGTCTGGTCGTGCTCGTACTCTAGTTGCAAGTTGGGAAACATGAGAAAAATCTCTCACTGCAGA
CAAACCTATATTGGTCAGTGGTCGTCCCACCACAAGTTATGGACTAGACATAAGCTCATTCATGTCGgtatggaatttttagttcctcacaagttggaaagcattcatttaaatgacaaaatttcttcaaggcgactgtccacactcttttggaagtgtgcttgtttaaattgttcttctattccagatttggcatttgg
ctttcctaaaatatcttttttctttttccaccattgcttaacattggcgatcatatatggttagtgcagAGAGGAAGCCAAGGGACTTCTGAAAGTGATTGGACAGTTAGTTATGATCAAAGTTCTATAGAAGACCTCATGCAACAACAC

Intron 3 GTCAGTGGTC ... GTTAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	2	0
A2	100	0	2	0
A3	-1	0	0	0
A4	-1	0	0	1
A5	-1	0	0	0
A6	66.67	6	12	0
A7	75	2	6	1
A8	54.55	5	6	7
A9	80	1	4	0
A10	100	0	3	1

>TRINITY_DN47149_c0_g1_i2 from sample A7 / Example spliced isoform

GGAGATGGCTATTGGCATTGATTCGTTAGGTTAAAAATATCTCATTATGTGGATGATGTTTGTACTTTGTTGCAATGCACATGGGTAAAATATCAATGGCTTTCCAGAATAAAAATATATGATTCCTCTAGTAGGTTCTCTGGGAGGTATCCAAAGTTCGGCTGGTTTATTGAAGATTCTTTCCGGTTGGTTGGTTGGCT
GCTTCTATTTCTGCATGTATGGGAACTTTTAGGTACAAAGACGCTTAGGAACAAAAGGAAGGATGTAGTCCAAGTTTCCAAGACAAATTCAAGCAACTTTCAACTATTTCCTTCTGGCATTCATGCATTAGTAGTGTTTTTCATGTGAAGGCATTTTGGGGTATTTCTCATTTGGTTCACATTCAAAGATCTTATTTTAA
TTTAGAAATTGTGCTTGAGCATTATTTTTTCTTTTTAAACCGCATGGCAGATGATGGAGTTGTATGTCAGCCAGATCAAGAAGTTACTGCGTCTAAATTATCAAAATGAGCAATGGTGGAGTCTGGTCGTGCTCGTACTCTAGTTGCAAGTTGGGAAACATGAGAAAAATCTCTCACTGCAGACAAACCTATATTGAGAG
GAAGCCAAGGGACTTCTGAAAGTGATTGGACAGTTAGTTATGATCAAAGTTCTATAGAAGACCTCATGCAACAACAC

>TRINITY_DN47149_c0_g1_i11 from sample A7 / Example unspliced isoform:

ATCACGGGGTCTATGCGGGTGCCGTCCCTGCCTGGTGTTCCGACTACATATGGCTCTGCCACTGTTGATGATGGAGTTGTATGTCAGCCAGATCAAGAAGTTACTGCGTCTAAATTATCAAAATGAGCAATGGTGGAGTCTGGTCGTGCTCGTACTCTAGTTGCAAGTTGGGAAACATGAGAAAAATCTCTCACTGCAGA
CAAACCTATATTGgtcagtggtcgtcccaccacaagttatggactagacataagctcattcatgtcggtatggaatttttagttcctcacaagttggaaagcattcatttaaatgacaaaatttcttcaaggcgactgtccacactcttttggaagtgtgcttgtttaaattgttcttctattccagatttggcatttgg
ctttcctaaaatatcttttttctttttccaccattgcttaacattggcgatcatatatggttagtgcagAGAGGAAGCCAAGGGACTTCTGAAAGTGATTGGACAGTTAGTTATGATCAAAGTTCTATAGAAGACCTCATGCAACAACAC

Intron 4 GTATTTAGAA ... CATGTATTAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.78	0.7	0	2
A2	100	0	0	2
A3	100	0	0	1
A4	-1	0	0	0
A5	-1	0	0	0
A6	18.1	7	0	3
A7	39.29	4.7	3	3
A8	47.37	5	3	6
A9	14.29	3	1	0
A10	95.24	0.3	9	3

>TRINITY_DN47149_c0_g1_i2 from sample A7 / Example spliced isoform

GGAGATGGCTATTGGCATTGATTCGTTAGGTTAAAAATATCTCATTATGTGGATGATGTTTGTACTTTGTTGCAATGCACATGGGTAAAATATCAATGGCTTTCCAGAATAAAAATATATGATTCCTCTAGTAGGTTCTCTGGGAGGTATCCAAAGTTCGGCTGGTTTATTGAAGATTCTTTCCGGTTGGTTGGTTGGCT
GCTTCTATTTCTGCATGTATGGGAACTTTTAGGTACAAAGACGCTTAGGAACAAAAGGAAGGATGTAGTCCAAGTTTCCAAGACAAATTCAAGCAACTTTCAACTATTTCCTTCTGGCATTCATGCATTAGTAGTGTTTTTCATGTGAAGGCATTTTGGGGTATTTCTCATTTGGTTCACATTCAAAGATCTTATTTTAA
TTTAGAAATTGTGCTTGAGCATTATTTTTTCTTTTTAAACCGCATGGCAGATGATGGAGTTGTATGTCAGCCAGATCAAGAAGTTACTGCGTCTAAATTATCAAAATGAGCAATGGTGGAGTCTGGTCGTGCTCGTACTCTAGTTGCAAGTTGGGAAACATGAGAAAAATCTCTCACTGCAGACAAACCTATATTGAGAG
GAAGCCAAGGGACTTCTGAAAGTGATTGGACAGTTAGTTATGATCAAAGTTCTATAGAAGACCTCATGCAACAACAC

>TRINITY_DN47149_c0_g1_i5 from sample A7 / Example unspliced isoform:

GGAAGGGCTTTGCAGAAACAGATTCCAAGTTTGCAACATTGGCTCCTTACATGTGTGGTGCTCGTTTGCTCAGATAGGATCTGCTTGAGTGCCTTTTTTAGTTTGGTTTCTCTTCAAATAATAATATTCATAGGATTACACAGATGGTGGGCTTTTTGTCTGGATTTGGACCTTGTTTGGGGACCGTGGAGGGGATTGAG
TTTCATGCTTTTCCTTCTTTGAAAGACTTGTCTTTGTTCGCTGAAGGGTAGCTTCTGAAGGTAGGATTCGGTTGCAGGTTTGATTCTCTCCCTTCTAGTTATTTATTTATTTTTAAAATGTAATTTAATATTTCCTCCTTGGGAGGTGCATCAAGAAATCCGAAATAGAGTTCGAGCTGCTCTATTGCAAATAACGGACT
TCATCTCTTTGAAACTGATGCCATAACAATGAGAGCATACCACAACTTGGACAGTTTAATACCTCGTCTTGCTCAGACCTTCTAATGAAGGCATGGTCATGGGTCAATCCAGCTAGCTTTGCCAACCAGCAACTTTCAACTATCTCCTTATTGCATTCATGCATTAGTAGTGTTTTTCATGTGAAGGCATTTTGGGGTAT
TTCTCATTTGGTTCACATTCAAAGATCTTATTTTAATTTAGAAATTGTGCTTGAGCATTATTTTTTCTTTTTAAACCGCATGGCAGATGATGGAGTTGTATGTCAGCCAGATCAAGAAGTTACTGCGTCTAAATTATCAAAATGAGgtatttagaagttggcttcattgattttcgttaatttttttctcttgatgatag
tgtagctttgttcaaatttgttcagcatattgtattgtaagtatagtattttgaaagattgagatatatattttgcatgtattagCAATGGTGGAGTCTGGTCGTGCTCGTACTCTAGTTGCAAGTTGGGAAACATGAGAAAAATCTCTCACTGCAGACAAACCTATATTGGTCAGTGGTCGTCCCACCACAAGTTATGG
ACTAGACATAAGCTCATTCATGTCGAGAGGAAGCCAAGGGACTTCTGAAAGTGATTGGACAGTTAGTTATGATCAAAGTTCTATAGAAGACCTCATGCAACAACAC