NA ----> NA

Intron 1 GTGGTCCGGG ... GGAAGACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	10.3	25	23.1
A2	64.17	29.8	58	44.4
A3	67.82	16.6	34	34
A4	52.73	23.4	31	19.4
A5	70.97	9	23	20.1
A6	60.22	418.3	705.8	526.8
A7	60.59	34.9	60	45.1
A8	62.03	39.3	67	53.8
A9	60.33	98.6	164	128.3
A10	72.44	15.2	38.1	37.9

>TRINITY_DN107951_c0_g1_i2 from sample A2 / Example spliced isoform

TCTTGTTCGAATTATCTACTGAGGTGTGTTGTTTCAAGTGTAGTGAGTTATACCCAGCAAGAATGGAGCTGCTTCGTGCCACTAGGGTTATGTATTTCTCTCTTGTGCTCGTCATTTTGTTTTCATTCAGCCTCACGATTATGAGCGATGTCCGCTATTCGGTGGAGGCATCAATGGATCATGGCGCCCAAGGTGGTCAC
GTCGTCGGAAAAGTTGACAGTTTTGGTCCGGGCGGTGGCGGTGGACGTGGAGGACCAGGCGGCGGTAGTCCTGGAGGAGGCGGTGGAGGACGCCGTCCCCCGGGAGGAGGAGGCGGTGGAGGACACCGTCCCCCGGGAGGAGGAGGCGGTGGTGGACGCGGTAGTCCGGGAGGAGGCGGGGGTCCGGGAGGCGGCGGCTG
AGGTCACGGTGGTCCCGGAGGAGGCAGAGGCGGCGGTGGTCTGGGATGAGGCGGTTACGGCTACTGCAGTTTGTGTTGGCCATGAAGTATAGCGTGTTGTGTATTCAGTGAATGTAATAATAATAAAAGGAATTCCGTCGATTATAGAAAGATGTGTATTCTGCTACGTAGTGTTATAATAATAACAAGCTGGGTTGCAA
CTTGCAAATCCCTGCTTTTTGTTTTTCGCAGTGTGTTTAAAAACCGTGATATATTCCTGGCATGGCCATTTTTGCTAT

>TRINITY_DN107951_c0_g1_i1 from sample A2 / Example unspliced isoform:

TCTTGTTCGAATTATCTACTGAGGTGTGTTGTTTCAAGTGTAGTGAGTTATACCCAGCAAGAATGGAGCTGCTTCGTGCCACTAGGGTTATGTATTTCTCTCTTGTGCTCGTCATTTTGTTTTCATTCAGCCTCACGATTATGAGCGATGTCCGCTATTCGGTGGAGGCATCAATGGATCATGGCGCCCAAGGTGGTCAC
GTCGTCGGAAAAGTTGACAGTTTTGGTCCGGGCGGTGGCGGTGGACGTGGAGGACCAGgtggtccggggggaggcggaagaccagGCGGCGGTAGTCCTGGAGGAGGCGGTGGTCCTGGAGGAGGCGGTGGAGGACGCCGTCCCCCGGGAGGAGGAGGCGGTGGAGGACACCGTCCCCCGGGAGGAGGAGGCGGTGGTGG
ACGCGGTAGTCCGGGAGGAGGCGGGGGTCCGGGAGGCGGCGGCTGAGGTCACGGTGGTCCCGGAGGAGGCAGAGGCGGCGGTGGTCTGGGATGAGGCGGTTACGGCTACTGCAGTTTGTGTTGGCCATGAAGTATAGCGTGTTGTGTATTCAGTGAATGTAATAATAATAAAAGGAATTCCGTCGATTATAGAAAGATGT
GTATTCTGCTACGTAGTGTTATAATAATAACAAGCTGGGTTGCAACTTGCAAATCCCTGCTTTTTGTTTTTCGCAGTGTGTTTAAAAACCGTGATATATTCCTGGCATGGCCATTTTTGCTAT

Intron 2 GTGGTCCGGG ... GGAAGACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.86	13	25	23
A2	59.3	35	58	44
A3	65.38	18	34	34
A4	48.57	27	31	20
A5	70.49	9	23	20
A6	56.31	469	695	514
A7	57.54	38	59	44
A8	54.93	48	65	52
A9	56.18	110	160	122
A10	68.81	17	38	37

>TRINITY_DN40787_c0_g1_i1 from sample A8 / Example spliced isoform

TCTTGTTCGAATTATCTACTGAGGTGTGTTGTTTCAAGTGTAGTGAGTTATACCCAGCAAGAATGGAGCTGCTTCGTGCCACTAGGGTTATGTATTTCTCTCTTGTGCTCGTCATTTTGTTTTCATTCAGCCTCACGATTATGAGCGATGTCCGCTATTCGGTGGAGGCATCAATGGATCATGGCGCCCAAGGTGGTCAC
GTCGTCGGAAAAGTTGACAGTTTTGGTCCGGGCGGTGGCGGTGGACGTGGAGGACCAGGCGGCGGTAGTCCTGGAGGAGGCGGTGGAGGACGCCGTCCCCCGGGAGGAGGAGGCGGTGGAGGACCCCGTCCCCCGGGAGGAGGAGGCGGTGGTGGACGCGGTAGTCCGGGAGGAGGCGGGGGTCCGGGAGGCGGCGGCTG
AGGTCACGGTGGTCCCGGAGGAGGCAGAGGAGGCGGTGGTCTGGGATGAGGCGGTTACGGCTACTGCAGTTTGTGTTGGCCATGAAGTATAGCGTGTTGTGTATTCAGTGAATGTAATAATAATAAAAGGAATTCCGTCGATTATAGAAAGATGTGTATTCTGCTACGTAGTGTTATAATAATAACAAGCTGGGTTGCAA
CTTGCAAATCCCTGCTTTTTGTTTTTCGCAGTGTGTTTAAAAACCGTGATAGATTCCTGGCATGGCCATTTTTGCTATAACATACATGATGATTTGATTTTTGCTATGTTTAGATATTTATAATGCACACGACTTAACTTGTCTTGGAATGTGATTTCAAGTTATGCAATGGAGC

>TRINITY_DN40787_c0_g1_i2 from sample A8 / Example unspliced isoform:

TCTTGTTCGAATTATCTACTGAGGTGTGTTGTTTCAAGTGTAGTGAGTTATACCCAGCAAGAATGGAGCTGCTTCGTGCCACTAGGGTTATGTATTTCTCTCTTGTGCTCGTCATTTTGTTTTCATTCAGCCTCACGATTATGAGCGATGTCCGCTATTCGGTGGAGGCATCAATGGATCATGGCGCCCAAGGTGGTCAC
GTCGTCGGAAAAGTTGACAGTTTTGGTCCGGGCGGTGGCGGTGGACGTGGAGGACCAGgtggtccgggggggaggcggaagaccagGCGGCGGTAGTCCTGGAGGAGGCGGTGGTCCTGGAGGAGGCGGTGGAGGACGCCGTCCCCCGGGAGGAGGAGGCGGTGGAGGACCCCGTCCCCCGGGAGGAGGAGGCGGGGGTC
CGGGAGGGGGCGGAGATGGCGGACGCGGGGGTCCAGTTGGCGGCGGAGGTGGCGGTGGTGGACGCGGTAGTCCGGGAGGAGGCGGGGGTCCGGGAGGCGGCGGAGGTGGCGGTGGAGGACGCCGTCCCCCGGGAGGAGGAGGCGGTGGCGGAGGTCACGGTGGTCCGGGAGGAGGCAGTGGCGGCGGTGGTCTGGGATGA
GGCGGTTACGGCTACTGCAGTTTGTGTTGGCCATGAAGTATAGCGTGTTGTGTATTCAGTGAATGTAATAATAATAAAAGGAATTCCGTCGATTATAGAAAGATGTGTATTCTGCTACGTAGTGTTATAATAATAACAAGCTGGGTTGCAACTTGCAAATCCCTGCTTTTTGTTTTTCGCAGTGTGTTTAAAAACCGTGA
TAGATTCCTGGCATGGCCATTTTTGCTATAACATACATGATGATTTGATTTTTGCTATGTTTAGATATTTATAATGCACACGACTTAACTTGTCTTGGAATGTGATTTCAAGTTATGCAATGGAGC