sp|Q8VY16|CDP1_ARATH ----> Plastid division protein CDP1, chloroplastic


Intron 1 GTGAGTTTAC ... TGTCCTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A120.04321.48
A26.0331.30.72
A318.62215
A427.8620.818
A57.0413.201
A66.8327.402
A79.1939.714
A813.9137.21.46
A934.6541.74.822
A1010.6442.42.45



>TRINITY_DN101774_c0_g1_i3 from sample A4 / Example spliced isoform
TCTGATCGTGTGTGAAGAAAATTTTATTGTAATTTTTTATGGGTAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGGTACATTCTATGATTCTACCTCTTCATGCAC
TTCTTTTTGAGTACTTGTAACGCCATTGGGATATTTCTTTCTGATGATACAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCAT
GTGCGGTTAGAGATTGGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTC
TCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTC
ACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCT
ATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAA
CTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTC
AACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAG
AGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG


>TRINITY_DN101774_c0_g1_i7 from sample A4 / Example unspliced isoform:
CGAGATTGTACGCCTTCTTTGgtgagtttactttaaaggggggcgcaggagatttctattttcgaaggggtcttgcaagtatctctgggtttgtattcaacgaaaaacattgatctgtcctgcagGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCTATGTTG
TTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCT
GGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCG
TAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTC
TGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG



Intron 2 GTGCAAGACA ... TCTTCCTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A167.07275852
A270.83215844
A368.36286358
A477.98166746.3
A578.6273813.5
A671.6236155
A763.18376661
A869.91174633
A965.953971.979.2
A1074.6164747



>TRINITY_DN101774_c0_g1_i1 from sample A4 / Example spliced isoform
GTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCCTTCTTGCTTTCTTGG
CTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTCTTCACTTCTCCATGC
CAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAAAGGACCAAGTAGTCA
AAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTC
CTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAG
CAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATT
GGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCT
GAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAA
AGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGCTGAAATC
CCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTAC
AACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTA
CACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATT
CTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAA
CCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACA
TCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTA
TATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTG
TGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGA
TTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAATTGAGACATGTCCAAT
CTTATTCAACTCTCGTGGACATTGTTTTGATCCTGGGTGTGTTATTTTTTTCTTAATCAATTTC


>TRINITY_DN101774_c0_g1_i17 from sample A4 / Example unspliced isoform:
GAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACT
GATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAG
CGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGT
AATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGG
CCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATG
AAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGgtgcaagacacacatcttcatcctatgctgcttcatcacaggcgcagagtttaatgcctactaatcctgttcaagctttcactttttcttcctcagCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAG
TCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTAT
TTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTC
AATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATC
AGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGA
GGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATT
GAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCT
TCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCT
TTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCAT
TCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAATTGAGACATGTCCAATCTTATTCAACTCTCGTGGACATTGTTTTGATCCTGGGTGTGTTA
TTTTTTTCTTAATCAATTTC



Intron 3 GTGAAAAGTC ... GACGGCACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A131.876632.829
A229.014519.817
A323.2233128
A435.27443117
A526.232810.99
A634.378051.832
A734.36876427
A835.94119.187.746
A937.379770.845
A1032.178348.730



>TRINITY_DN127426_c0_g1_i10 from sample A1 / Example spliced isoform
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCT
GCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCA
GTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGGTACATTCTATGATTCTACCTCTTCATGCACTTCTTTTTGAGTACTTGTAACGCCATTGGGATATTTCTTTCTGATGATACAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAAC
ACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACT
CTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAG
GGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATT
GTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAA
GGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTA
TAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGC
AGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCA
GAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAA
TTACTATAGGTGAGTTTACTGCATTGTAGGAGTTAAGTGTGTTTTTCATATTTTAGGAGTCAACAAGATACATGATGAACAGGACTTAAAAATGACATTTAGAGTTTGCGCTTTAACCTATAAACTTTGTAGTCATGTCTGATGTTCATACTGCTTTTGGTGTTTTGGACCAATACAGCACGTATCACATAAGGTATGTT
TTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAA
CAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGAT
TGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTG
CCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAAC
TTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT


>TRINITY_DN127426_c0_g1_i9 from sample A1 / Example unspliced isoform:
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCT
GCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCA
GTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCAT
GTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTC
TCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTC
ACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACAT
CTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCA
CGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCC
AAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCA
GGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGgtgaaaagtccagaactaatgcaactatgatactgcgtgttgattttgatcacctgctttcattgttttctgctagctatcttacag
ctttctggcttgggcatttatgacggcacagTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGC
TCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTT
GTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAA
TGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGAC
AGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCT
ATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT



Intron 4 GTGGGTTCTG ... CTTATGCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A140.911309
A227.781305
A318.18902
A437.51006
A50900
A66.671421
A79.521912
A821.431113
A941.8625118
A1028.8932013



>TRINITY_DN44030_c0_g1_i21 from sample A9 / Example spliced isoform
CCGGGAAGCGGGGGATTTACATCGACGGCGGACAGTGTCCCCGTACGTCATCCCGTCACCATCGTTTCAGCCTGCGAAGTTAATGGTGGGTTTTAAAACTCCGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTAC
TTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGC
AAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGC
ACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGT
TGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG


>TRINITY_DN44030_c0_g1_i11 from sample A9 / Example unspliced isoform:
CCGGGAAGCGGGGGATTTACATCGACGGCGGACAGTGTCCCCGTACGTCATCCCGTCACCATCGTTTCAGCCTGCGAAGTTAATGGTGGGTTTTAAAACTCCGgtgggttctgtgcatagcgtttggtatctttcatcgctaacctgtgcaattttgtttgcctagcagatttggcttttcttttggataatattgcaca
ttgccgtggaacggtatggcaatgcttgtgttgcttaaagttggaactgtgtcttatgccagAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTGAGTTTACTTTAAAGGGGGGCGCAGGAGATTTCTATTTTCGAAGGGGT
CTTGCAAGTATCTCTGGGTTTGTATTCAACGAAAAACATTGATCTGTCCTGCAGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAA
ATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATT
TACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCAT
TTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAG
ATTCTTGCAGAGCCCATGCTCTCTCAG



Intron 5 GTATGTTTTT ... GCATTTACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A117.0765.6270
A224.07411610
A321.1546.6232
A423.36411510
A517.073477
A628.571557
A718.2838125
A815.152855
A916.0534103
A1020.831964



>TRINITY_DN45214_c0_g1_i4 from sample A6 / Example spliced isoform
AGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAG
CCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAG
AGATTGGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGC
GCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCA
GATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTG
CAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAAT
GTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGT
AAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAAC
AAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGGTGAAAAGTCCAGAACTAATGCAACTATGATACTGCGTGTTGATTTTGATCACCTGCTTTCATTGTTTGTGGTGC
AATTTTGTATCCATATTTAGGATTGCATGTTCAGCAGGGATGGGAAATCATGCCTGAGACTGGATGTAAACTTGAGATAGTCAAATAATTCTGGTGTTTTGGTTTGAAGTTTCAAAGAGTTTATTCCGTACATTTTCATACCAAAATCCCATCCACATCTAAACTTTGGGTTATATTTTTCTCATTTATTGTTGCGCTCG
TCTTTCTTTTCAACTAAGAGGATTGACCTTTTGAGAGTATCACTAAATTATATCCATTGTCAATCTAGGTATCTTCTCCAATGAGAATCAAACTGTACTATCACAGTATTACTTCTCTTCAAATTTAAAAGTGAAATGTGTTGTTCTGTTGAATGTTAATATAAAAAGCAC


>TRINITY_DN45214_c0_g1_i24 from sample A6 / Example unspliced isoform:
CAACAACATTCCAGTTCACTCGAAGGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCT
CCTGGTTTTGGTTCACGGATCCCCTTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCA
TAATGGGTCTCTGCCCTTGAGTTCTTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTT
TAGGAATACCTATTGGGTCTTCAAAGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTT
ATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGG
ATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGAC
AAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTG
TTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGgtatgttttttgttttggttgtttctggtgctttgaaggactgttctctgacagaattccagtctttgctgattttgcaattgcatttacagATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTT
GAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATG
CTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCT
TTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGC
TGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGG
CAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAAC
CATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGA
ACTTGTTGACGAGTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGT
TTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTT
TTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGAC
ACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACA
GACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTA
CTAATGACCAATTGAGACATGTCCAATCTTATTCAACTCTCGTGGACATTGTTTTGATCCTGGGTGTGTTATTTTTTTCTTAATCAATTTCAGAAGCCG



Intron 6 GTACATTCTA ... GATGATACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A131.09411324
A241.07331630
A333.82451927
A432.61311020
A532.39241013
A631.911669
A736.47271318
A837.51066
A941.94181115
A1039.13211017



>TRINITY_DN45214_c0_g1_i23 from sample A6 / Example spliced isoform
CAACAACATTCCAGTTCACTCGAAGGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCT
CCTGGTTTTGGTTCACGGATCCCCTTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCA
TAATGGGTCTCTGCCCTTGAGTTCTTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTT
TAGGAATACCTATTGGGTCTTCAAAGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGTTTATTGGTTTTGAGGGCTTGCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGA
GCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTT
TTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTA
ACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGA
TTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGC
TTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGC
TTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAA
ATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCAC
AGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTG
ATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATG
CTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGT
GGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCC
TTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCA
ACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCG
GTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCC
GTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAATTGAGACATGTCCAATCTTATTCAACTCTCGTGGACATTGTTTTGATCCTGGGTGTGTTATTTTTTTCTTAATCAATT
TCAGAAGCCG


>TRINITY_DN45214_c0_g1_i6 from sample A6 / Example unspliced isoform:
CAACAACATTCCAGTTCACTCGAAGGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCT
CCTGGTTTTGGTTCACGGATCCCCTTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCA
TAATGGGTCTCTGCCCTTGAGTTCTTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTT
TAGGAATACCTATTGGGTCTTCAAAGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTT
ATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGG
ATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGgtacattctatgattctacctcttcatgcacttctttttgagtacttaacgccattgggatatttctttctgatgatacagATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAG
AAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCT
CTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAAT
CTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATT
GTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAA
GGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTA
TAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGC
AGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCA
GAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAA
TTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAA
ACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTAC
ATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTAC
CCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGA
GTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAATTGAGACATGTCCAATCTTATT
CAACTCTCGTGGACATTGTTTTGATCCTGGGTGTGTTATTTTTTTCTTAATCAATTTCAGAAGCCG



Intron 7 GTACATTCTA ... GATGATACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A137.4940.212.224
A248.2232.315.530
A338.3843.717.927
A439.6230.59.520
A535.523.39.512.7
A636.5215.75.69
A740.6626.412.518
A839.219.55.86
A946.5617.410.415
A1045.2520.69.417



>TRINITY_DN127426_c0_g1_i11 from sample A1 / Example spliced isoform
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGTACTGTAAATCGAAAAACCTAATGTTTGTGTGAGAGAGAGAAAATATGAACAGAAAATATGTGATGGTATAAATGAAGGTTTATTGGTTTTGAGGGCTTGCAGGAGTTGTTGA
TAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATT
CATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGA
AAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTC
TAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATC
TGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGC
TCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCT
TCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGC
AGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGT
CTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCAT
CGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGA
GTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTT
TCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGAC
CTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATG
GGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGA
GAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT


>TRINITY_DN127426_c0_g1_i10 from sample A1 / Example unspliced isoform:
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCT
GCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCA
GTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGgtacattctatgattctacctcttcatgcacttctttttgagtacttgtaacgccattgggatatttctttctgatgatacagATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAAC
ACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACT
CTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAG
GGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATT
GTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAA
GGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTA
TAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGC
AGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCA
GAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAA
TTACTATAGGTGAGTTTACTGCATTGTAGGAGTTAAGTGTGTTTTTCATATTTTAGGAGTCAACAAGATACATGATGAACAGGACTTAAAAATGACATTTAGAGTTTGCGCTTTAACCTATAAACTTTGTAGTCATGTCTGATGTTCATACTGCTTTTGGTGTTTTGGACCAATACAGCACGTATCACATAAGGTATGTT
TTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAA
CAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGAT
TGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTG
CCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAAC
TTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT



Intron 8 GTACTGTAAA ... GGGCTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A13513410
A231.821568
A317.3938106
A4202055
A530.8628916
A624.321445
A716.422829
A89.09501
A921.9532117
A1015.561952



>TRINITY_DN127426_c0_g1_i10 from sample A1 / Example spliced isoform
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCT
GCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCA
GTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGGTACATTCTATGATTCTACCTCTTCATGCACTTCTTTTTGAGTACTTGTAACGCCATTGGGATATTTCTTTCTGATGATACAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAAC
ACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACT
CTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAG
GGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATT
GTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAA
GGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTA
TAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGC
AGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCA
GAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAA
TTACTATAGGTGAGTTTACTGCATTGTAGGAGTTAAGTGTGTTTTTCATATTTTAGGAGTCAACAAGATACATGATGAACAGGACTTAAAAATGACATTTAGAGTTTGCGCTTTAACCTATAAACTTTGTAGTCATGTCTGATGTTCATACTGCTTTTGGTGTTTTGGACCAATACAGCACGTATCACATAAGGTATGTT
TTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAA
CAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGAT
TGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTG
CCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAAC
TTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT


>TRINITY_DN127426_c0_g1_i11 from sample A1 / Example unspliced isoform:
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGgtactgtaaatcgaaaaacctaatgtttgtgtgagagagagaaaatatgaacagaaaatatgtgatggtataaatgaaggtttattggttttgagggcttgcagGAGTTGTTGA
TAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATT
CATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGA
AAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTC
TAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATC
TGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGC
TCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCT
TCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGC
AGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGT
CTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCAT
CGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGA
GTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTT
TCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGAC
CTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATG
GGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGA
GAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT



Intron 9 GTAAGGCATG ... AAATATGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A113.984094
A222.58361110
A311.113235
A431.581393
A518.1827102
A615.151450
A7162162
A814.291541
A911.112852
A1017.071761



>TRINITY_DN101774_c0_g1_i11 from sample A4 / Example spliced isoform
GTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCCTTCTTGCTTTCTTGG
CTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTCTTCACTTCTCCATGC
CAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAAAGGACCAAGTAGTCA
AAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTC
CTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAG
CAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATT
GGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCT
GAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAA
AGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGA
CACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGG
GGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATC
AGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATG
GAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGC
TGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGC
TCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAA
GGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAG
TTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCT
CATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGA
ACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAATTGAGACATGTCCAATCTTATTCAACTCTCGTGGACATTGTTTTGATCCTGGGTGTGTTATTTTTTTCTTAATCAATTTC


>TRINITY_DN101774_c0_g1_i9 from sample A4 / Example unspliced isoform:
GTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCCTTCTTGCTTTCTTGG
CTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTCTTCACTTCTCCATGC
CAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAAAGGACCAAGTAGTCA
AAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTC
CTGCAAGAAgtaaggcatgcgcaactgttttttattttattttagggtagattttgcgttttctttgcttttgggatgctttctcaaattggttgttaggaatggggatgattgtaattagttaatcacaaatggagaaatatgcagGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACC
CAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGGTAAAGAACCTTCAAACCTCTTTCTTGCCCATTTTTAATAGTAGGTGGC



Intron 10 GTGGGTTTTA ... CTTATGCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A165.6311021
A258.0613018
A3559011
A462.59015
A535.71905
A6559211
A746.6716114
A857.149012
A960.4219129
A1054.6929035



>TRINITY_DN45214_c0_g1_i11 from sample A6 / Example spliced isoform
GGGATTTACATCGACGGCGGACAGTGTCCCCGTACGTCATCCCGTCACCATCGTTTCAGCCTGCGAAGTTAATGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAA
TGGCTTGAAGAAAAAAGGGTTTGAACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGT
CTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCC
GGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG


>TRINITY_DN45214_c0_g1_i16 from sample A6 / Example unspliced isoform:
GGGATTTACATCGACGGCGGACAGTGTCCCCGTACGTCATCCCGTCACCATCGTTTCAGCCTGCGAAGTTAATGgtgggttttaaaactccggtgggttctgtgcatagcgtttggtatctttcatcgctaacctgtgcaattttgtttgcctagcagatttggcttttcttttggataatattgcacattgccgtggaa
cggtatggcaatgcttgtgttgcttaaagttggaactgtgtcttatgccagAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTG
AACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACA
GCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGA
TTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG



Intron 11 GTGAGTTTAC ... ACCAATACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A18.164547.8
A27.145245
A313.4171118.1
A47.323833.1
A502104.1
A618.751433318
A711.21111416.3
A818.0518139.924.2
A913.731762818
A1016.2110219.79.3



>TRINITY_DN127426_c0_g1_i11 from sample A1 / Example spliced isoform
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGTACTGTAAATCGAAAAACCTAATGTTTGTGTGAGAGAGAGAAAATATGAACAGAAAATATGTGATGGTATAAATGAAGGTTTATTGGTTTTGAGGGCTTGCAGGAGTTGTTGA
TAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATT
CATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGA
AAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTC
TAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATC
TGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGC
TCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCT
TCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGC
AGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGT
CTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCAT
CGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGA
GTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTT
TCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGAC
CTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATG
GGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGA
GAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT


>TRINITY_DN127426_c0_g1_i10 from sample A1 / Example unspliced isoform:
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCT
GCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCA
GTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGGTACATTCTATGATTCTACCTCTTCATGCACTTCTTTTTGAGTACTTGTAACGCCATTGGGATATTTCTTTCTGATGATACAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAAC
ACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACT
CTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAG
GGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATT
GTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAA
GGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTA
TAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGC
AGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCA
GAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAA
TTACTATAGgtgagtttactgcattgtaggagttaagtgtgtttttcatattttaggagtcaacaagatacatgatgaacaggacttaaaaatgacatttagagtttgcgctttaacctataaactttgtagtcatgtctgatgttcatactgcttttggtgttttggaccaatacagCACGTATCACATAAGGTATGTT
TTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAA
CAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGAT
TGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTG
CCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAAC
TTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT



Intron 12 GTAAGTTTCC ... TATTGTCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A144.8722.72016.8
A231.51291115.7
A337.330.91224.8
A427.6533.91411.9
A527.9419.611.14
A630.791979.9
A741.27281326.3
A837.0815.999.8
A933.8929.9921.7
A1037.77231017.9



>TRINITY_DN127426_c0_g1_i10 from sample A1 / Example spliced isoform
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCT
GCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCA
GTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGGTACATTCTATGATTCTACCTCTTCATGCACTTCTTTTTGAGTACTTGTAACGCCATTGGGATATTTCTTTCTGATGATACAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAAC
ACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACT
CTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAG


GGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATT
GTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAA
GGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTA
TAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGC
AGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCA
GAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAA
TTACTATAGGTGAGTTTACTGCATTGTAGGAGTTAAGTGTGTTTTTCATATTTTAGGAGTCAACAAGATACATGATGAACAGGACTTAAAAATGACATTTAGAGTTTGCGCTTTAACCTATAAACTTTGTAGTCATGTCTGATGTTCATACTGCTTTTGGTGTTTTGGACCAATACAGCACGTATCACATAAGGTATGTT
TTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAA
CAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGAT
TGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTG
CCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAAC
TTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT


>TRINITY_DN127426_c0_g1_i6 from sample A1 / Example unspliced isoform:
GAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACT
GATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAG
CGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGT
AATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGgtaagtttccttagcaatccaagctatttaaaaaattg
tctaattttttgatatattcttactattgtccagGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTG



Intron 13 GTTCCAAGTC ... GTCCTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A164.7234.544.881
A265.4832.541.581
A368.6525.248.860
A472.1815.129.948
A578.076.614.932
A673.8728.759.8102
A766.6743.173.399.1
A868.4828.338.984.1
A965.955181.9115
A1065.3441.537.8118



>TRINITY_DN127426_c0_g1_i5 from sample A1 / Example spliced isoform
GAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACT
GATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAG
CGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGT
AATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGG
CCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATG
AAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGCTCTGTA
TTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACC
ATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGG
AGATTCTTGCAGAGCCCATGCTCTCTCAG


>TRINITY_DN127426_c0_g1_i11 from sample A1 / Example unspliced isoform:
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGTACTGTAAATCGAAAAACCTAATGTTTGTGTGAGAGAGAGAAAATATGAACAGAAAATATGTGATGGTATAAATGAAGGTTTATTGGTTTTGAGGGCTTGCAGGAGTTGTTGA
TAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATT
CATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGA
AAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTC
TAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATC
TGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGC
TCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCT
TCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGgttccaagtcattcaattttcagcaaatctaaaaatgcttcatcgtttaaatggaagcaaaatgttggggtcccacgaaagaaactttctggaagtttgtcggggaagagtaaaatagctggaaaattctttttggctgctgtccttgc
agGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGT
CTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCAT
CGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGA
GTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTT
TCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGAC
CTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATG
GGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGA
GAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT



Intron 14 GTAAAGAACC ... TTATGGGTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A120.4139105.6
A239.772315.212.4
A334.563619.77.4
A413.641936.4
A538.151173.6
A620.2882.20.8
A730.07156.54.8
A845.510.99.21.6
A920.95226.25.6
A1040.21106.86.6



>TRINITY_DN127426_c0_g1_i10 from sample A1 / Example spliced isoform
GGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCTCCTGGTTTTGGTTCACGGATCCCC
TTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCATAATGGGTCTCTGCCCTTGAGTTC
TTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTTTAGGAATACCTATTGGGTCTTCAA
AGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCT
GCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCA
GTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGGTACATTCTATGATTCTACCTCTTCATGCACTTCTTTTTGAGTACTTGTAACGCCATTGGGATATTTCTTTCTGATGATACAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAAC
ACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACT
CTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAG
GGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATT
GTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAA
GGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTA
TAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGC
AGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCA
GAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAA
TTACTATAGGTGAGTTTACTGCATTGTAGGAGTTAAGTGTGTTTTTCATATTTTAGGAGTCAACAAGATACATGATGAACAGGACTTAAAAATGACATTTAGAGTTTGCGCTTTAACCTATAAACTTTGTAGTCATGTCTGATGTTCATACTGCTTTTGGTGTTTTGGACCAATACAGCACGTATCACATAAGGTATGTT
TTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAA
CAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGAT
TGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTG
CCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAAC
TTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAAT


>TRINITY_DN127426_c0_g1_i12 from sample A1 / Example unspliced isoform:
TGCGTTTTCTTTGCTTTTGGGATGCTTTCTCAAATTGGTTGTTAGGAATGGGGATGATTGTAATTAGTTAATCACAAATGGAGAAATATGCAGGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGgt
aaagaaccttcaaacctctttcttgcccatttttaatagtaggtggctcctctatatctgatcgtgtgtgaagaaaattttattgtaattttttatgggtagTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTT
GGCAAAGCTGATTCTGTTAGCAGAGGTACATTCTATGATTCTACCTCTTCATGCACTTCTTTTTGAGTACTTGTAACGCCATTGGGATATTTCTTTCTGATGATACAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGG
AGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTG
TCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTC
CAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTG
GCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCA
AAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGG
TCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCA
TCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG



Intron 15 GTGGGTTTTA ... TGCCTAGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A145.45605
A236.36704
A325301
A450606
A5-1000
A625321
A723.081013
A822.22702
A933.331216
A1057.14608



>TRINITY_DN45214_c0_g1_i12 from sample A6 / Example spliced isoform
GGGATTTACATCGACGGCGGACAGTGTCCCCGTACGTCATCCCGTCACCATCGTTTCAGCCTGCGAAGTTAATGATTTGGCTTTTCTTTTGGATAATATTGCACATTGCCGTGGAACGGTATGGCAATGCTTGTGTTGCTTAAAGTTGGAACTGTGTCTTATGCCAGAAGACGTGCTGAAATTGATCAGGAAAAATGGCT
GAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGA
AATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGG
AAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTA
TCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG


>TRINITY_DN45214_c0_g1_i16 from sample A6 / Example unspliced isoform:
GGGATTTACATCGACGGCGGACAGTGTCCCCGTACGTCATCCCGTCACCATCGTTTCAGCCTGCGAAGTTAATGgtgggttttaaaactccggtgggttctgtgcatagcgtttggtatctttcatcgctaacctgtgcaattttgtttgcctagcagATTTGGCTTTTCTTTTGGATAATATTGCACATTGCCGTGGAA
CGGTATGGCAATGCTTGTGTTGCTTAAAGTTGGAACTGTGTCTTATGCCAGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTG
AACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACA
GCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGA
TTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG



Intron 16 GTTTATTGGT ... GGGCTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A142.2213712
A236.171589
A340.16382823
A440.3201413
A541.05282019
A641.67141010
A745.1282125
A847.37545
A943.36322623
A1033.3319109



>TRINITY_DN45214_c0_g1_i1 from sample A6 / Example spliced isoform
CAACAACATTCCAGTTCACTCGAAGGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCT
CCTGGTTTTGGTTCACGGATCCCCTTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCA
TAATGGGTCTCTGCCCTTGAGTTCTTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTT
TAGGAATACCTATTGGGTCTTCAAAGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTT
ATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTTTTGAAAGGGGCACAGTTTCAGAAGG
ATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGAC
AAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTG
TTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAA
TCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATC
CACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAAATCTAAAAATGCTTCATCGTTTAAA
TGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATC
CATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCAT
TTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATGCTTTTGGAGATTTGTGCTCCTGCAT
TCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGTGGAAATTTTGTGGAGCTGGCATTCA
ATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCCTTTCTAGATTAATTGCTTTTGGTCT
CTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCAACTGGAGAGAGTCTCCATTGTTTCC
TGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCGGTCTCCAAAGTTGTTGATTATGTTC
TTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCCGTTGGACACAACGAGGGCAATTTCA
GGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAATTGAGACATGTCCAATCTTATTCAACTCTCGTGGACATTGTTTTGATCCTGGGTGTGTTATTTTTTTCTTAATCAATTTCAGAAGCCG


>TRINITY_DN45214_c0_g1_i23 from sample A6 / Example unspliced isoform:
CAACAACATTCCAGTTCACTCGAAGGTGAAAGGGTCTTAGTTTTGGGTTTCAATATTGACTTTTTGGTCTCCAACACGCCTGGAGAACAGTTGAGGTAGAATACATATACGAAAATCATCGGTAATGGTGATATATTTATGGATTTAAGCATAAAATACCACACAATTAGCGACATCACGTTTGCACACGATCTGCTTCT
CCTGGTTTTGGTTCACGGATCCCCTTCTTGCTTTCTTGGCTGCAAGCGAGAACGAAAAATACAATCTGTGAAGAGCACCCACATCCATCCATTGTTCTTACGATGTACGAATGAATCTGTGGAAGAAGCATGAGCAAGATGGCTCTATCCTGCCTTGCATCGAACCATTTGGGTTACAGTTTTCCAAGGAAGCAGAAGCA
TAATGGGTCTCTGCCCTTGAGTTCTTCACTTCTCCATGCCAAGATTTCCCTGTTTTATCTCATGGGCAGATGCCATCCAAGAAAACGAGGGCCTGGATCTCTCACTCGGGCTCTAGATATCAAGGTAGCCGACACCCCTATAGTCGAAAATGGGCAGTTCCAAGGGGCCGTGGAGGTCCCTGTAAACTGTTATCAGATTT
TAGGAATACCTATTGGGTCTTCAAAGGACCAAGTAGTCAAAGCGGCCATGCAGCTGAGGGCAACTGAGATTGAGGAAGGGTACAGTCCTTCTGTTCTGCCCTCTCGTCAGgtttattggttttgagggcttgcagGAGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGA
GCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGTGCTCGATAGCGAGAAGAGGTT
TTGAAAGGGGCACAGTTTCAGAAGGATTCGAAGCTCTTGCTCGGGCTCAGTTGCTGCTGAGGAGCAAGAGATCTTTGGCAAAGCTGATTCTGTTAGCAGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTA
ACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCCGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGA
TTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTCCAAAAGC
TTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGC
TTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCAAAGGTTCCAAGTCATTCAATTTTCAGCAA
ATCTAAAAATGCTTCATCGTTTAAATGGAAGCAAAATGTTGGGGTCCCACGAAAGAAACTTTCTGGAAGTTTGTCGGGGAAGAGTAAAATAGCTGGAAAATTCTTTTTGGCTGCTGTCCTTGCAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCAC
AGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTG
ATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAGTGGCAAGCTCTGGCAGAATCAGCAAAATCGAGTTCATG
CTTTTGGAGATTTGTGCTCCTGCATTCATCTATAGTGCATGCTGAAATTTCATCCGACGACATTGGTTGGGAAATTGCAGAAGTGGACGCAGTATTGGAAGAAGCAGCTGAACTTGTTGACGAGTCTCAGCTCAAAAACCCAAATTACTATAGCACGTATCACATAAGGTATGTTTTAAAGAGGGGCAGTGATAGAAGGT
GGAAATTTTGTGGAGCTGGCATTCAATCTCCAGTCTAGAGGCTCAAATGGGGGGGTGCTGACTGATTTGATTTCTTGAAGATTTTTACATGATCTCTGTACGCATAGATGTTTCAAGAGCTTTTCTTGCTTGTGGACATCCTAAACCCATAAAATTTGGGATGAGAACGGCGAAACAGGATTGAAACCAGGGATAAGTCC
TTTCTAGATTAATTGCTTTTGGTCTCTTAAGAGAGAATGGAAGGGAACAGAGTAGGAGTGAGGAATAGTTTAATCCAGATTGTCCATTCTTTGGTGTCATTTGCAGGTGTTTTTTTAGTCGACCTGCAGAATGTTGTATATTACATGGCTCCTCTTAGAACATGGAGTTGTTGATTGTTTCTTCTAACTCGATCGCTGCA
ACTGGAGAGAGTCTCCATTGTTTCCTGTATTAGCAATTAGAGTTGAGAATAATTTTGTGTGTTAATATTAGAGTCTGGCTTGTGGGTGCTACAATCTATATATCCAGTGACACCACACAAATGGGCCAGACAGGGTTGTGGTACCCAGAATCAATTTGTATTTATAAAGGATTTGCCAGGCTTTATATACTTTGACTGCG
GTCTCCAAAGTTGTTGATTATGTTCTTTTCCTGTTACCTGCTCATGGATTATAAGAACTGAACGGCTTTCAGGAATTTTCACTGAAAGACAATTTTGTTTGCATCGCCACAGACCAAAAAGGAGAATCTCTATTCAGATTTTGAGTTTTTGACTTTGCCAAAATATTTTTGGAACTTCTCATTCTTGAAGATAACATGCC
GTTGGACACAACGAGGGCAATTTCAGGGCTTTTTATTTTGGAACACATTTGGCAAATATATACGTTCTGAAGTTTATCATGTGTGGCAACATTGTTGCAGATTTCATTGTACTAATGACCAATTGAGACATGTCCAATCTTATTCAACTCTCGTGGACATTGTTTTGATCCTGGGTGTGTTATTTTTTTCTTAATCAATT
TCAGAAGCCG



Intron 17 GTAAAGAACC ... GATGATACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A141.8323.71024
A247.4251530
A337.4938.318.727
A436.5120320
A543.7112.76.713
A634.0910.729
A747.1513.76.318
A840.741196
A950.4210.75.715
A1048.7212.7717



>TRINITY_DN127426_c0_g1_i15 from sample A1 / Example spliced isoform
AGTTGTTGATAGATATACGGGATAAACTTCTCTTTGAGCCAGAGTTTGCAGGAGATGTAAAGGAGCATGTTCCCCCAAAACCTTCCCTTATTCTTCCATGGCAATGGCTGCCTGCTGCAATGTCTCTCCTGCAAGAAGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAG
CCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGGAGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAG
AGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTGTCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGC
GCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGTAAGTTTCCTTAGCAATCCAAGCTATTTAAAAAATTGTCTAATTTTTTGATATATTCTTACTATTGTCCAGGGCAAACTTGCACTCACTGAG
GATCTCCAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTG


>TRINITY_DN127426_c0_g1_i12 from sample A1 / Example unspliced isoform:
TGCGTTTTCTTTGCTTTTGGGATGCTTTCTCAAATTGGTTGTTAGGAATGGGGATGATTGTAATTAGTTAATCACAAATGGAGAAATATGCAGGTCGGGGAGGAGAAAATTGTCATGGACATAGGCAGAGCAGTTAGGCAACAACCCAATGCCAAGCCCTACATTCATGATTTCCTTCTTTCCATGGCATTGGCTGAGgt
aaagaaccttcaaacctctttcttgcccatttttaatagtaggtggctcctctatatctgatcgtgtgtgaagaaaattttattgtaattttttatgggtagtgctcgatagcgagaagaggttttgaaaggggcacagtttcagaaggattcgaagctcttgctcgggctcagttgctgctgaggagcaagagatcttt
ggcaaagctgattctgttagcagaggtacattctatgattctacctcttcatgcacttctttttgagtacttgtaacgccattgggatatttctttctgatgatacagATTGAGGCATCTCTCGAGGAACTTGCACCAATATGCTTGCTAGAAAATCTAAGCAAGCCGCGAACACCGGAGAATGTAGAAAGAAGAGAGGG
AGCCTTAACAGCGTTGCGTGAGTTGCTGAGACAAGGCCTAGAAGGTGAAGTTTCATGTGCGGTTAGAGATTGGTCAGAGTTTCTTAGTCAAGCACTGAAGAAATTGCTGGCAATTGAAGTCGTTGGTTTGCTTCCTTGGGAAACGATTGCTCTAATTCGAAAGAACAATAACTCTTCAGAAGCTCAGCAACAACGGTTTG
TCATAGATTGTAATTGCTTCTACATAGCTCTGTTAGCTCATATTGCGTTGGGCTTCTCAAACAAGCGCCCTGAACTGATTGAGAAGGCCAAGAAGATCACAGAGAGCTTAGAAAAGACTGATGCAGTGAACTTGGGTTTTGAGAGAATAATCTGTAAAACACTTCTAGGAGAGGGCAAACTTGCACTCACTGAGGATCTC
CAAAAGCTTGGCCTGAACGGGAACTCCTTAAATCAACATTTTGTTCAAGATGTTTCACGCCAAGCAGATAAAGGAAATGCAGAATTCTATTCATCCTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTG
GCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCACAAATAGGTGCAAGACACACATCTTCATCCTATGCTGCTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGGTGGTCCAGATCAAATTTCTCCTTCA
AAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGG
TCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCA
TCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG



Intron 18 GTATCTTTCA ... CTTATGCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A155.3111015
A251.199111.5
A341.66706.5
A448.7510010.5
A525502.5
A629.161136
A728.891618
A838.811017.5
A955.8418123.5
A1044.4827224



>TRINITY_DN45214_c0_g1_i15 from sample A6 / Example spliced isoform
GGGATTTACATCGACGGCGGACAGTGTCCCCGTACGTCATCCCGTCACCATCGTTTCAGCCTGCGAAGTTAATGGTGGGTTTTAAAACTCCGGTGGGTTCTGTGCATAGCGTTTGAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCT
TTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGGCTCTGTATTTA
CAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTT
ACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGATTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGAT
TCTTGCAGAGCCCATGCTCTCTCAG


>TRINITY_DN45214_c0_g1_i16 from sample A6 / Example unspliced isoform:
GGGATTTACATCGACGGCGGACAGTGTCCCCGTACGTCATCCCGTCACCATCGTTTCAGCCTGCGAAGTTAATGGTGGGTTTTAAAACTCCGGTGGGTTCTGTGCATAGCGTTTGgtatctttcatcgctaacctgtgcaattttgtttgcctagcagatttggcttttcttttggataatattgcacattgccgtggaa
cggtatggcaatgcttgtgttgcttaaagttggaactgtgtcttatgccagAAGACGTGCTGAAATTGATCAGGAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTTAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTG
AACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCTTCCTCAGCTGAAATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGGCTCTGTATTTACAACCTATAAGTTGCATGTGCAATCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACA
GCCAGATTGCACAATGCGAACATCCATGGATGCTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAGGAGGATAACCATGGCAATCGCAAGGTCTTTGATACCATTTACACAGCAGAGCCAAAATCATAATGAACAAAATGCTTGGCCGGTCAATGATCTTTCCCTGTTGA
TTAGGAAGTCAGGAAATAATGCATTTCGTAGAAAACAAATGGAATTCAGGGAAGCAGAGAGTCTGGTCAGGCGGTGGCAAAGTATCAAAGCTGAAGCCTTGGGACCAAACCATGAAATCCATCGCCTTTCGGAGATTCTTGCAGAGCCCATGCTCTCTCAG



Intron 19 GCTCTGTATT ... GCATGTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A183.8752329
A272.6102231
A355.17131121
A451.52898
A584.62147
A660.92171934
A770153733
A856.86222434
A959.73303554
A1066.04183040



>TRINITY_DN45090_c0_g2_i16 from sample A8 / Example spliced isoform
GAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCT
TCCTCAGCTGAAATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGTCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATCGCACAATCCGAACATCCATGGATGTTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCACAGTCTTAGTGGAAACATTGGAAG


>TRINITY_DN45090_c0_g2_i6 from sample A8 / Example unspliced isoform:
GAAAAATGGCTGAAGGATGCTGCACTTGGGCTCTTTGTGGATACTCGAGATTGTACGCCTTCTTTGGTAAACTACTTTGGCGGCAATATGAAGATGTCTGTAAATGGCTTGAAGAAAAAAGGGTTTGAACATCCTATGTTGTTTCATCACAGGCGCAGAGTTTAATGCCTACTAATCCTGTTCAAGCTTTCACTTTTTCT
TCCTCAGCTGAAATCCCTGAAGCTGGTCCAGATCAAATTTCCCCTTCAAAGgctctgtatttacaacctataagttgcatgtgcagTCTAGACCAATTACACGTCTATTTGAAAGTCATACTGCACAGCCAGATCGCACAATCCGAACATCCATGGATGTTGTAAATCAGCGGCCAAGCTGTTCAACTCGTAATGCCCAC
AGTCTTAGTGGAAACATTGGAAG