sp|Q9ZUZ2|CAMK3_ARATH ----> CDPK-related kinase 3

Intron 1 GCTCTGCGAA ... AAAGCCAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.03	16	12	16
A2	51.52	10	9	11
A3	56.4	8.5	8	16
A4	48.68	12	5.5	16.5
A5	53.89	9.5	5.5	17
A6	44.71	18	20	12
A7	43.85	26.5	19	24.5
A8	41.66	16.5	11	14
A9	51.22	29	10	42.5
A10	52.7	21.5	17	34.5

>TRINITY_DN98143_c1_g2_i6 from sample A4 / Example spliced isoform

TTCCCCAAAAGCGCCTCCAAATCTGTTCCCGTCTCGTCCCAAAACCCCGGCAACAAATGGGCGGCATTCCTGCAGTTGGTCCCCTCACTTTTTTTATAATACAGCAGTATCCATGCCAGGCAGCGAGAATGAAGACTCGGAGAGTCGGAGCTCTGCGCCGGGTCTCCAAATTCATCCTCTATGGGGAATTTCCGGTTTTT
CTTTCTGTTCAGTACCTTACATCTCGGTACCATCCCAAGAAACCGGTCAAACGGCAAGGCTGAGCAACCTTCCGTTGGCAGGATGCTCAGAAAACTCGACGGAAACCTTCCTATTTCTCTAATCCAGTAAGTGTTGAAAACCTTTAGTTCAATTTGAAGATTAAAAAAAAAGCCCGATTAAGCTTGAAATTCTCCCAGGC
CTGTTATGGCACAATCGGTAGAGCAGAGAGAAATGTACGGCCTCGGTTTTCCCCGACCTTCAACGCCTCACTGAAAAAAATAAGGTTTCTATCGTAAGACGTCCAGGGTTTGGATATTAAAGAATCACACGACATGTGAATTGATTCAGATATAGACGTAAAAATATACTTGGCCTGGATTTTCCCCGAACTTTGATGGT
GAACTGAAAAATTATCGGGTTTCCAAGGTCACGTTCTCAATACGTCTAGGGTTTATCAAGGAATTTCCGGACCTGTTTGAATCAATTCGGAGATATATGTAAAACAAGCGCATTTGGATTCAATGGGCCAATGCTATGGGAAAGATGTCGAGGAGCATACATTTGTAGTTCAAAAAGCGGTCACTGATACGTCCCAGGAT
CAACCGTTGTCCACTCCATTGCATTACTTAAATAACACGTATTCATACGCTCCCAGTCCTTTGACAAGCCCGCTTCCCGTAGGCGCTTCTCCTTCCCCCGCTAGAGCGACCCCCCGGAGGTTTTTTCGCAGGCCTTTCCCCCCTCCTTCTCCTGCCAAGCATATATACTCTTCTCTTGTGAAGAGACATGGAGTTCCCAA
GCGCAAGGAGGCCTCAGTACCCGAGGTGGCCCCTGTGGAGGGCGAGAAACCCCTTGATAAGAGTTTTGGTTACAGTAAAAGTTTTGGTGCCAAGTATGAGCTTCGGCAGGAAATTGGGCGTGGACATTTTGGGCATACGTGTTATGCAAAAGTGTGCAAGGGGGAGCTTAAGGGGCAGTCTGTGGCCGTGAAGATCATCT
CCAAAGAGAAGATGACGACAGCTATTGCAATCGAAGATGTGCGGAGAGAAGTTAAGATTTTGAAAGCATTGTCAGGGCATCATAATTTGGTCATATTTCACGATGTATGCGAGGATGACAACAATGTCTACATTATCATGGAGCTCTGCGAAGGTGGGGAACTGTTGGACAGAATTTTGTCCAGTGGTGGAAGGTTCTCA
GAGGAAGATGCCAAGGGTATTGTTGTGCAGATTTTGAGCGTAGTTGCCTTTTGTCACCTTCAGGGGGTGGTGCATCGTGATTTAAAGCCAGAGAATTTTCTCTTCACAACAAAAGATGAAAATGCACCTTTGAAGGCTATTGATTTTGGGCTGTCAGATTTCATTAGACCAGATGAAAGACTGAATGATATTGTAGGCAG
CGCATATTATGTAGCACCAGAAGTCCTTCATCAATCGTATAGTATGGAAGCGGATGTATGGAGTATTGGTGTAATAACATACATCCTACTATGTGGTAGTCGACCTTTCTGGGCACAGGCGGAATCAGGAATTTTTCGTGCGGTTTTGAGAGCTGAACCTAATTTTGATGATGCACCATGGCCTTCTATCTCTCCTGAGG
CTAAGGATTTTGTGAAAAGGCTTCTTAACAAAGATTTGCGAAAACGAATGAGTGCGGCTCAGGCCATTTCACATCCATGGCTTCGTGGGAAACATGTAATTCCATTAGATATTTCGTTGTACAAACAAGTTAAAGCTTATCTCCGCTACTTGCCATTAAAACGTGCAGCTCTCAAGGCACTGGTGAAATCATTAAGAGAA
GATGACTTGGTATATCTCAGAGCTCAGTTTAAGCTCCTGCAACCTGATAACAATGCCAGGATTTCTCTAGAGAATTTTAAATCCGCTCTAATGAGGAATTCAACAGAGGCCATGAAGGAATCCAAGGTTCCAGATATACTAAATTCGATGAATCTGCTTGCTTTCAGAAAGATGAACTTTGAGGAGTTCTGTGCTGCTGC
AACAAGTGTATATCAACTAGAAGCCCTTGATCACTGGGAGGAAGTCGCACGGTCTGCATTTGCATTCTTTGAACAACAGGGCAATAGAGTTATTTCAGTTGAAGAGTTGGCTCAGGAGTTGAACCTTGGGCCAACTGTCTATGCAGCTTTGCATGATTGGATTAGGCACACAGATGGGAAACTCAGCTTTTTTGGCTTCA
CCAAGTTTCTGCATGGTGTTACACTGCGATCAAATATGAGGACACAACGCTTACACTGCTAGTTATTTAACATTTGTAATTTTTTAATTTAACTATAGACAACTTTGGGGTCCCTTTTTCCTGTTTTGGAGTGTGTTTCATCAGTTTCCACAGGGATAACCACAGTAGTCTCCTCTTAGAATTCCTCGTGGGAGTATTTT
CCTTCGAAT

>TRINITY_DN98143_c1_g2_i3 from sample A4 / Example unspliced isoform:

TTTCTCTTCACAACAAAAGATGAAAATGCACCTTTGAAGGCTATTGATTTTGGGCTGTCAGATTTCATTAGACCAGATGAAAGACTGAATGATATTGTAGGCAGCGCATATTATGTAGCACCAGAAGTCCTTCATCAATCGTATAGTATGGAAGCGGATGTATGGAGTATTGGTGTAATAACATACATCCTACTATGTGG
TAGTCGACCTTTCTGGGCACAGGCGGAATCAGGAATTTTTCGTGCGGTTTTGAGAGCTGAACCTAATTTTGATGATGCACCATGGCCTTCTATCTCTCCTGAGGCTAAGGATTTTGTGAAAAGGCTTCTTAACAAAGATTTCCGAAAACGAATGAGTGCGGCTCAGGCCATTTCACATCCATGGCTTCGTGGGAAACATG
TAATTCCATTAGATATTTCGTTGTACAAACAAGTTAAAGCTTATCTCCGCTACTTGCCATTAAAACGTGCAGCTCTCAAGgctctgcgaaggtggggaactgttggacagaattttgtccagtggtggaaggttctcagaggaagatgccaagggtattgttgtgcagattttgagcgtagttgccttttgtcaccttca
gggggtggtgcatcgtgatttaaagccagagGCACTGGTGAAATCATTAAGAGAAGATGACTTGGTATATCTCAGAGCTCAGTTTAAGCTCCTGCAACCTGATAACAATGCCAGGATTTCTCTAGAGAATTTTAAATCCGCTCTAATGAGGAATTCAACAGAGGCCATGAAGGAATCCAAGGTTCCAGATATACTAAATT
CGATGAATCTGCTTGCTTTCAGAAAGATGAACTTTGAGGAGTTCTGTGCTGCTGCAACAAGTGTATATCAACTAGAAGCCCTTGATCACTGGGAGGAAGTCGCACGGTCTGCATTTGCATTCTTTGAACAACAGGGCAATAGAGTTATTTCAGTTGAAGAGTTGGCTCAGGAGTTGAACCTTGGGCCAACTGTCTATGCA
GCTTTGCATGATTGGATTAGGCACACAGATGGGAAACTCAGCTTTTTTGGCTTCACCAAGTTTCTGCATGGTGTTACACTGCGATCAAATATGAGGACACAACGCTTACACTGCTAGTTATTTAACATTTGTAATTTTTTAATTTAACTATAGACAACTTTGGGGTCCCTTTTTCCTGTTTTGGAGTGTGTTTCATCAGT
TTCCACAGGGATAACCACAGTAGTCTCCTCTTAGAATTCCTCGTGGGAGTATTTTCCTTCGAAT

Intron 2 GTGCAGCGAA ... ATACTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.64	29	3.3	10
A2	18.18	18	6.3	4
A3	20	32	3.7	8
A4	16.14	19	4	3.7
A5	7.14	13	0.7	1
A6	27.25	56	12.7	21
A7	28.53	85	11.7	34
A8	35.29	55	9.3	30
A9	23.89	70	14.7	22
A10	23.96	73	6.7	23

>TRINITY_DN41641_c0_g2_i3 from sample A6 / Example spliced isoform

GTTCCCCAAAAGCGCCTCCAAATCTGTCCCCGTCTCGTCCCAAAACCCCGGCAACAAATGGGCGGCATTCCTGCAGTTGGTCCCCTCACTTTTTTTATAATACAGCAGTATCCATGCCAGGCAGCGAGAATGAAGACTCGGAGAGTCGGAGCTCTGCGCCGGGTCTCCAAATTCATCCTCTATGGGGAATTTCCGGTTTT
TCTTTCTGTTCAGTACCTTACATCTCGGTACCATCCCAAGAAACCGGTCAAACGGCAAGGCTGAGCAACCTTCCGTTGGCAGGATGCTCAGAAAACTCGACGGAAACCTTCCTATTTCTCTAATCCAGTAAGTGTTGAAAACCTTTAGTTCAATTTGAAGATTAAAAAAAAAGCCCGATTAAGCTTGAAATTCTCCCAGG
CCTGTTATGGCACAATCGGTAGAGCAGAGAGAAATGTACGGCCTCGGTTTTCCCCGACCTTCAACGCCTCACTGAAAAAAATAAGGTTTCTATCGTAAGACGTCCAGGGTTTGGATATTAAAGAATCACGCGACATGTGAATTGATTCAGATATAGACGTAAAAATATACTTGGCCTGGATTATCCCCGAACTTTGATGG
TGAACTGAAAAATTATCGGGTTTCCAAGGTCACGTTCTCAATACGTCCAGGGTTTATCAAGGAATTTCCGGACCTGTTTGAATCAATTCGGAGATATATGTAAAACAAGCGCATTTGGATTCAATGGGCCAATGCTATGGGAAAGATGTCGAGGAGCATACATTTGTAGTTCAAAAAGCGGTCACTGACACGTCCCAGGA
TCAACCGTTGTCCACTCCATTGCATTACTTAAATAACACGTATTCATACGCTCCCAGCCCTTTGACAAGCCCGCTTCCCGTAGGCGCTTCTCCTTCCCCCGCTAGAGCGACCCCCCGGAGGTTTTTTCGCAGGCCTTTCCCCCCTCCTTCTCCTGCCAAGCATATATACTCTTCTCTTGTGAAGAGACATGGAGTTCCCA
AGCGCAAGGAGGCCTCAGTACCCGAGGTGGCCCCTGTGGAGGGCGAGAAACCCCTTGATAAGAGTTTTGGTTACAGTAAAAGTTTTGGTGCCAAGTATGAGCTTCGGCAGGAGATTGGGCGTGGACATTTTGGGCATACGTGTTATGCAAAAGTGTGCAAGGGGGAGCTTAAGGGGCAGTCTGTGGCCGTGAAGATCATC
TCCAAAGAGAAGATGACGACAGCTATTGCAATCGAAGATGTGCGGAGAGAAGTTAAGATTTTGAAAGCATTGTCAGGGCATCATAATTTGGTCATATTTCACGATGTATGCGAGGATGACAACAATGTCTACATTATCATGGAGTATTACTTTATTCCTTACATAAGTGTAAATTTGTTTTTCTCTTATGACTTGACTCT
TATGTTCTAGATTCAGGAAGAAGATGCAAATTTGATATTGAAATATTGTCAGAAAACTTAATTATAAGACTTGGGTATTTTTGGTAGTTGCG

>TRINITY_DN41641_c0_g2_i2 from sample A6 / Example unspliced isoform:

AGGGTTTGGATATTAAAGAATCACGCGACATGTGAATTGATTCAGATATAGACGTAAAAATATACTTGGCCTGGATTATCCCCGAACTTTGATGGTGAACTGAAAAATTATCGGGTTTCCAAGGTCACGTTCTCAATACGTCCAGGGTTTATCAAGGAATTTCCGGACCTGTTTGAATCAATTCGGAGATATATGTAAAA
CAAGCGCATTTGGATTCAATGGGCCAATGCTATGGGAAAGATGTCGAGGAGCATACATTTGTAGTTCAAAAAGCGGTCACTGACACGTCCCAGGATCAACCGTTGTCCACTCCATTGCATTACTTAAATAACACGTATTCATACGCTCCCAGCCCTTTGACAAGCCCGCTTCCCGTAGGCGCTTCTCCTTCCCCCGCTAG
AGCGACCCCCCGGAGGTTTTTTCGCAGGCCTTTCCCCCCTCCTTCTCCTGCCAAGCATATATACTCTTCTCTTGTGAAGAGACATGGAGTTCCCAAGCGCAAGGAGGCCTCAGTACCCGAGGTGGCCCCTGTGGAGGGCGAGAAACCCCTTGATAAGAGTTTTGGTTACAGTAAAAGTTTTGGTGCCAAGTATGAGCTTC
GGCAGGAGATTGGGCGTGGACATTTTGGGCATACGTGTTATGCAAAAGTGTGCAAGGGGGAGCTTAAGGGGCAGTCTGTGGCCGTGAAGATCATCTCCAAAGAGAAGgtgcagcgaatggataatgctgttatactgacagATGACGACAGCTATTGCAATCGAAGATGTGCGGAGAGAAGTTAAGATTTTGAAAGCATT
GTCAGGGCATCATAATTTGGTCATATTTCACGATGTATGCGAGGATGACAACAATGTCTACATTATCATGGAGTATTACTTTATTCCTTACATAAGTGTAAATTTGTTTTTCTCTTATGACTTGACTCTTATGTTCTAGATTCAGGAAGAAGATGCAAATTTGATATTGAAATATTGTCAGAAAACTTAATTATAAGACT
TGGGTATTTTTGGTAGTTGCG