sp|O04350|TBCA_ARATH ----> Tubulin-folding cofactor A

Intron 1 GTTAGCACGG ... TTATTCGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.36	18.3	18.6	39.7
A2	81.29	15.6	37.6	92
A3	74.03	29.3	57	104.6
A4	78.71	16.2	33	75.3
A5	69.33	16.7	19	52.3
A6	70.71	164.4	247.3	507.9
A7	72.47	144.6	219.6	480.4
A8	73.04	94.4	139.8	331.3
A9	73.72	130.9	211.1	484.1
A10	74.25	103	194.2	374.3

>TRINITY_DN117138_c0_g1_i8 from sample A1 / Example spliced isoform

TGGTAATATCAACTTAAGTTATAAACGACGCCGGGGAAGAGAAAGAACTAGCGACTCCAGCTGTATTATTATACAGGGTTAAGGGTGTTTTAGAAAAGCTCTTCTCTGAACGAGGAGACCTTTACCTGCTCAAATTAGATGCAGCTAAGTTCCCGGAACGACTAAGTCATGGCAACTCTGAAAACTCTCAAGATCAAGAC
AGGCACCTGTAAGCGAGTCCTCAAAGAGCTTCATTCATATGAAAAGGAGGTTGACAGGGAAGCTGCAAAGACTGCTGACATGAAAGAGAAGGGTGCTGATCCATATGACCTTAAGCAACAGGAGAATGTACTAGCAGAATCAAGGATGATGCTTCCTGATTGTCGGAAACGCCTTGAAGCTGCCCTTATTGACTTGCAGG
GAATGCTGGCAGAGCTAGAGCAGTCAGGTCAGGAAGGTGATGAGGTTCGTGAAGCCAAAACTGTAGTAAAAGACATAGAACCTTTGTTTGAGATTCCTGCATCATGAGGGCACTCAGTTTTTCTATGAAAGTAACATACTTTATACAAAGTTGCTTTGAGAATTTTTACTGATTCTTGGAGGCCTGTTTGAGAACATAGC
ATTACTATGCCTTGCTGTTTACTTGAAAACACAACCTTCGAGCTGCTTTTTAGATGTTTGTACTAAAGCCCTCAAAAGAGGTATCTTGACTCTTATTGGTTAGGATGTGTGCTTTGACAATGGGTATATGATATTTCTATCTTTTGTTTATAAGATCAGCCGCGGACTACGAATACTGATTTTATCCTTATTAAGCACCA
TAGTTGAAGTGCTTCTTTGTGTTTTCAATGTTTTTTAATTATTGCA

>TRINITY_DN117138_c0_g1_i1 from sample A1 / Example unspliced isoform:

TGGTAATATCAACTTAAGTTATAAACGACGCCGGGGAAGAGAAAGAACTAGCGACTCCAGCTGTATTATTATACAGGgttagcacggaaagtagctttctttgtttttaagccataccctccaatttcaagcgctgaaagtgtttgttgaattcactgttttattcgtagGTTAAGGGTGTTTTAGAAAAGCTCTTCTCT
GAACGAGGAGACCTTTACCTGCTCAAATTAGATGCAGCTAAGGTTGCCAACTTGTGTGTCACTCGAGTAAAGTATACTAGACTTTTTTTCTATACAGTTCCCGGAACGACTAAGTCATGGCAACTCTGAAAACTCTCAAGATCAAGACAGGCACCTGTAAGCGAGTCCTCAAAGAGCTTCATTCATATGAAAAGGAGGTT
GACAGGGAAGCTGCAAAGACTGCTGACATGAAAGAGAAGGGTGCTGATCCATATGACCTTAAGCAACAGGAGAATGTACTAGCAGAATCAAGGATGATGCTTCCTGATTGTCGGAAACGCCTTGAAGCTGCCCTTATTGACTTGCAGGGAATGCTGGCAGAGCTAGAGCAGTCAGGTCAGGAAGGTGATGAGGTTCGTGA
AGCCAAAACTGTAGTAAAAGACATAGAACCTTTGTTTGAGATTCCTGCATCATGAGGGCACTCAGTTTTTCTATGAAAGTAACATACTTTATACAAAGTTGCTTTGAGAATTTTTACTGATTCTTGGAGGCCTGTTTGAGAACATAGCATTACTATGCCTTGCTGTTTACTTGAAAACACAACCTTCGAGCTGCTTTTTA
GATGTTTGTACTAAAGCCCTCAAAAGAGGTATCTTGACTCTTATTGGTTAGGATGTGTGCTTTGACAATGGGTATATGATATTTCTATCTTTTGTTTATAAGATCAGCCGCGGACTACGAATACTGATTTTATCCTTATTAAGCACCATAGTTGAAGTGCTTCTTTGTGTTTTCAATGTTTTTTAATTATTGCA

Intron 2 GTATGATTCA ... AGATACACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.44	148.3	39	12.1
A2	15.24	135	25	9.2
A3	21.9	123.6	35.6	14.6
A4	16.5	89	18.3	8.9
A5	23.51	62.9	19.6	10.9
A6	17.98	1261.5	283.3	131.6
A7	16.74	1054.9	217.2	92.3
A8	14.75	982.7	173.8	86
A9	15.77	1065.3	205	61.3
A10	19.45	680.4	168.8	41

>TRINITY_DN117138_c0_g1_i7 from sample A1 / Example spliced isoform

TGGTAATATCAACTTAAGTTATAAACGACGCCGGGGAAGAGAAAGAACTAGCGACTCCAGCTGTATTATTATACAGGGTTAGCACGGAAAGTAGCTTTCTTTGTTTTTAAGCCATACCCTCCAATTTCAAGCGCTGAAAGTGTTTGTTGAATTCACTGTTTTATTCGTAGGTTAAGGGTGTTTTAGAAAAGCTCTTCTCT
GAACGAGGAGACCTTTACCTGCTCAAATTAGATGCAGCTAAGGTTGCCAACTTGTGTGTCACTCGAGTAAAGTATACTAGACTTTTTTTCTATACAGTTCCCGGAACGACTAAGTCATGGCAACTCTGAAAACTCTCAAGATCAAGACAGGCACCTGTAAGCGAGTCCTCAAAGAGCTTCATTCATATGAAAAGGAGGTT
GACAGGGAAGCTGCAAAGACTGCTGACATGAAAGAGAAGGGTGCTGATCCATATGACCTTAAGCAACAGGAGAATGTACTAGCAGAATCAAGGATGATGCTTCCTGATTGTCGGAAACGCCTTGAAGCTGCCCTTATTGACTTGCAGGGAATGCTGGCAGAGCTAGAGCAGTCAGGTCAGGAAGGTGATGAGGTTCGTGA
AGCCAAAACTGTAGTAAAAGATGTAGAACCTTTGTTTGAGACTCATGCGTCATGAGGGCACTCAGTTTTTCTATGAGAATAACATACTTTATACAAAGTTACCTTGAGAATTTTTACTGATTCTTGGAGGCCTGTTTGAGAACACAGTATTACTCTAATCTCAAATTTAAAATGTTTGGCTTGTTGGGAATCTGGGATAA
GCC

>TRINITY_DN117138_c0_g1_i11 from sample A1 / Example unspliced isoform:

TGGTAATATCAACTTAAGTTATAAACGACGCCGGGGAAGAGAAAGAACTAGCGACTCCAGCTGTATTATTATACAGGATTAGCACGGAAAGTAGCTTTCTTTGTTTTTAAGCCATACCCTCCAATTTCAAGCGCTGAAAGTGTTTGTTGAATTCACTGTTTTATTCGTAGGTTAAGGGTGTTTTAGAAAAGTTCTTCTCT
GAACGAGGAGACCTTTACCTGCTCAAATTAGATGCAGCTAAGGTTGCCAACTTGTGTGTCACTCGAGTAAAGTATACTAGACTTTTTTTCTATACAGTTCCCGGAACGACTAAGTCATGGCAACTCTGAAAACTCTCAAGATCAAGACAGGCACCTGTAAGCGAGTCCTCAAAGAGCTTCATTCATATGAAAAGGAGGTT
GACAGGGAAGCTGCAAAGACTGCTGACATGAAAGAGAAGGGTGCTGATCCATATGACCTTAAGCAACAGGAGAATGTACTAGCAGAATCAAGGATGATGCTTCCTGATTGTCGGAAACGCCTTGAAGCTGCCCTTATTGACTTGCAGGGAATGCTGgtatgattcagtgtcctctctctaaaggaaaaaagatacacagG
CAGAGCTAGAGCAGTCAGGTCAGGAAGGTGATGAGGTTCGTGAAGCCAAAACTGTAGTAAAAGATGTAGAACCTTTGTTTGAGACTCATGCGTCATGAGGGCACTCAGTTTTTCTATGAGAATAACATACTTTATACAAAGTTACCTTGAGAATTTTTACTGATTCTTGGAGGCCTGTTTGAGAACACAGTATTACTCTA
ATCTCAAATTTAAAATGTTTGGCTTGTTGGGAATCTGGGATAAGCC

Intron 3 GTTGCCAACT ... TTCTATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.5	43.1	51.8	99.6
A2	65.1	55	71.3	102.3
A3	61.55	67	69.4	107.7
A4	68.13	41.8	58.9	88.9
A5	64.92	28	42.2	53.7
A6	65.67	304.1	449.8	580.7
A7	65.05	283.1	384.9	524.8
A8	63.47	255.6	288.6	449.1
A9	67.27	272.9	401.3	561.1
A10	67.72	186.2	305.2	393.9

>TRINITY_DN117138_c0_g1_i8 from sample A1 / Example spliced isoform

TGGTAATATCAACTTAAGTTATAAACGACGCCGGGGAAGAGAAAGAACTAGCGACTCCAGCTGTATTATTATACAGGGTTAAGGGTGTTTTAGAAAAGCTCTTCTCTGAACGAGGAGACCTTTACCTGCTCAAATTAGATGCAGCTAAGTTCCCGGAACGACTAAGTCATGGCAACTCTGAAAACTCTCAAGATCAAGAC
AGGCACCTGTAAGCGAGTCCTCAAAGAGCTTCATTCATATGAAAAGGAGGTTGACAGGGAAGCTGCAAAGACTGCTGACATGAAAGAGAAGGGTGCTGATCCATATGACCTTAAGCAACAGGAGAATGTACTAGCAGAATCAAGGATGATGCTTCCTGATTGTCGGAAACGCCTTGAAGCTGCCCTTATTGACTTGCAGG
GAATGCTGGCAGAGCTAGAGCAGTCAGGTCAGGAAGGTGATGAGGTTCGTGAAGCCAAAACTGTAGTAAAAGACATAGAACCTTTGTTTGAGATTCCTGCATCATGAGGGCACTCAGTTTTTCTATGAAAGTAACATACTTTATACAAAGTTGCTTTGAGAATTTTTACTGATTCTTGGAGGCCTGTTTGAGAACATAGC
ATTACTATGCCTTGCTGTTTACTTGAAAACACAACCTTCGAGCTGCTTTTTAGATGTTTGTACTAAAGCCCTCAAAAGAGGTATCTTGACTCTTATTGGTTAGGATGTGTGCTTTGACAATGGGTATATGATATTTCTATCTTTTGTTTATAAGATCAGCCGCGGACTACGAATACTGATTTTATCCTTATTAAGCACCA
TAGTTGAAGTGCTTCTTTGTGTTTTCAATGTTTTTTAATTATTGCA

>TRINITY_DN117138_c0_g1_i1 from sample A1 / Example unspliced isoform:

TGGTAATATCAACTTAAGTTATAAACGACGCCGGGGAAGAGAAAGAACTAGCGACTCCAGCTGTATTATTATACAGGGTTAGCACGGAAAGTAGCTTTCTTTGTTTTTAAGCCATACCCTCCAATTTCAAGCGCTGAAAGTGTTTGTTGAATTCACTGTTTTATTCGTAGGTTAAGGGTGTTTTAGAAAAGCTCTTCTCT
GAACGAGGAGACCTTTACCTGCTCAAATTAGATGCAGCTAAGgttgccaacttgtgtgtcactcgagtaaagtatactagactttttttctatacagTTCCCGGAACGACTAAGTCATGGCAACTCTGAAAACTCTCAAGATCAAGACAGGCACCTGTAAGCGAGTCCTCAAAGAGCTTCATTCATATGAAAAGGAGGTT
GACAGGGAAGCTGCAAAGACTGCTGACATGAAAGAGAAGGGTGCTGATCCATATGACCTTAAGCAACAGGAGAATGTACTAGCAGAATCAAGGATGATGCTTCCTGATTGTCGGAAACGCCTTGAAGCTGCCCTTATTGACTTGCAGGGAATGCTGGCAGAGCTAGAGCAGTCAGGTCAGGAAGGTGATGAGGTTCGTGA
AGCCAAAACTGTAGTAAAAGACATAGAACCTTTGTTTGAGATTCCTGCATCATGAGGGCACTCAGTTTTTCTATGAAAGTAACATACTTTATACAAAGTTGCTTTGAGAATTTTTACTGATTCTTGGAGGCCTGTTTGAGAACATAGCATTACTATGCCTTGCTGTTTACTTGAAAACACAACCTTCGAGCTGCTTTTTA
GATGTTTGTACTAAAGCCCTCAAAAGAGGTATCTTGACTCTTATTGGTTAGGATGTGTGCTTTGACAATGGGTATATGATATTTCTATCTTTTGTTTATAAGATCAGCCGCGGACTACGAATACTGATTTTATCCTTATTAAGCACCATAGTTGAAGTGCTTCTTTGTGTTTTCAATGTTTTTTAATTATTGCA