sp|P45986|IMT1_MESCR ----> Inositol 4-methyltransferase

Intron 1 GTCTAGGCAC ... ATTATTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	92.66	3	18	58.5
A2	100	0	16	41
A3	94.97	2	19	56.5
A4	100	0	16	60.5
A5	100	0	8	19.5
A6	74.36	5	7	22
A7	80.4	12	38	60.5
A8	68.94	17	35	40.5
A9	70.62	13	37	25.5
A10	68.95	17	28	47.5

>TRINITY_DN43443_c5_g3_i1 from sample A9 / Example spliced isoform

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGG
GAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

>TRINITY_DN43443_c5_g3_i2 from sample A9 / Example unspliced isoform:

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGg
tctaggcactggatccgccttgaagaaccttcacacactggtggcataaatcttgatctccaacacgttattgatctaaatttgttgatgtattaggatagaaactggatgataatttttagaaattattacagGGAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATG
ATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

Intron 2 GTCTAGGCAC ... CATGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	95.67	1.5	18	49
A2	98.11	0.5	16	35.5
A3	94.51	2	19	50
A4	90.11	4	16	57
A5	100	0	8	17
A6	76.71	5	7	23
A7	76.92	14.5	38	58.5
A8	72.73	14	35	38
A9	68.05	15	37	25.5
A10	68.67	17	28	44.5

>TRINITY_DN43443_c5_g3_i12 from sample A9 / Example spliced isoform

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGG
ATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

>TRINITY_DN43443_c5_g3_i2 from sample A9 / Example unspliced isoform:

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGg
tctaggcactggatccgccttgaagaaccttcacacactggtggcataaatcttgatctccaacacgttattgatctaaatttgttgatgtattaggatagaaactggatgataatttttagaaattattacagggaataagtgcatgttgcagGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATG
ATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

Intron 3 GTGTGTTTTG ... CATGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	96.4	1.5	8	40
A2	98.38	0.5	26	30
A3	95.83	2	7	46
A4	93.1	4	12	54
A5	100	0	7	16
A6	83.66	5	3	24
A7	78.87	14.5	8	54
A8	73	14	9	37
A9	65.68	15	3	28
A10	73.48	17	9	46

>TRINITY_DN43443_c5_g3_i12 from sample A9 / Example spliced isoform

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGG
ATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

>TRINITY_DN43443_c5_g3_i13 from sample A9 / Example unspliced isoform:

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGg
tgtgttttgtttacactctccctgtctttaattttcattttcattggtgtgtttttgtttgcatgtccaggcactttgttttctttagttttcttttctttcgattctttttgtttgcaggtctaggcactggatccgccttgaagaaccttcacacactggtggcataaatcttgatctccaacacgttattgatctaa
atttgttgatgtattaggatagaaactggatgataatttttagaaattattacagggaataagtgcatgttgcagGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATT
GCTTATGAATCCACGGCCAAA

Intron 4 GTGTGTTTTG ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.86	18	8	87
A2	75	16	26	48
A3	72.46	19	7	50
A4	76.47	16	12	52
A5	75.76	8	7	25
A6	80	7	3	28
A7	64.49	38	8	69
A8	50.7	35	9	36
A9	55.95	37	3	47
A10	56.25	28	9	36

>TRINITY_DN43443_c5_g3_i2 from sample A9 / Example spliced isoform

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGG
TCTAGGCACTGGATCCGCCTTGAAGAACCTTCACACACTGGTGGCATAAATCTTGATCTCCAACACGTTATTGATCTAAATTTGTTGATGTATTAGGATAGAAACTGGATGATAATTTTTAGAAATTATTACAGGGAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATG
ATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

>TRINITY_DN43443_c5_g3_i13 from sample A9 / Example unspliced isoform:

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGg
tgtgttttgtttacactctccctgtctttaattttcattttcattggtgtgtttttgtttgcatgtccaggcactttgttttctttagttttcttttctttcgattctttttgtttgcagGTCTAGGCACTGGATCCGCCTTGAAGAACCTTCACACACTGGTGGCATAAATCTTGATCTCCAACACGTTATTGATCTAA
ATTTGTTGATGTATTAGGATAGAAACTGGATGATAATTTTTAGAAATTATTACAGGGAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATT
GCTTATGAATCCACGGCCAAA

Intron 5 GTGTGTTTTG ... ATTATTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	94.44	3	8	51
A2	100	0	26	36
A3	96.43	2	7	54
A4	100	0	12	58
A5	100	0	7	19
A6	81.48	5	3	22
A7	82.86	12	8	58
A8	69.64	17	9	39
A9	65.79	13	3	25
A10	73.85	17	9	48

>TRINITY_DN43443_c5_g3_i1 from sample A9 / Example spliced isoform

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGG
GAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

>TRINITY_DN43443_c5_g3_i13 from sample A9 / Example unspliced isoform:

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGg
tgtgttttgtttacactctccctgtctttaattttcattttcattggtgtgtttttgtttgcatgtccaggcactttgttttctttagttttcttttctttcgattctttttgtttgcaggtctaggcactggatccgccttgaagaaccttcacacactggtggcataaatcttgatctccaacacgttattgatctaa
atttgttgatgtattaggatagaaactggatgataatttttagaaattattacagGGAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATT
GCTTATGAATCCACGGCCAAA

Intron 6 GTGAGGAAAT ... TTTTTGCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.17	3.8	40.5	35
A2	94.91	1.3	27	19
A3	84.98	5.5	32	24
A4	80.08	6.5	22	18
A5	93.73	0.5	5	9
A6	34.81	12	10	3
A7	54.86	15	16.5	21
A8	62.58	13.8	17	27
A9	53.08	16	17.5	19
A10	59.62	15.8	22.5	23

>TRINITY_DN43443_c5_g3_i1 from sample A9 / Example spliced isoform

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGG
GAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

>TRINITY_DN43443_c5_g3_i3 from sample A9 / Example unspliced isoform:

GAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAATATGTTCGAGCAGTTACCTTCCGCGGACGCAATGATGATGAAGgtgaggaaatcatctcatttgcataacaaatttacatatgattgtgttattattcctacatgatgaaggtgaccatatcctgattacaatggattatacatgattgggaggacgaa
gactgtcaaattgctgagaaaagctacgaagaaacactagcaaacggaaatgtcttcatttcaaccctggcgagggagtggtgaaattaagactcaaccaatttttttcatagagaaattgacatggcaaaagctcccatgacatgggaaacatagagaaattgacatggcaaaagagtgactcgaatgtgcgtggctgc
aatatgtagtgtaggggaaatcaccaccttaataaaaacgttttttcattctttgctttttgctagCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

Intron 7 GTGAGGAAAT ... TTTTTGCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.95	3.8	48	35
A2	95.42	1.3	32	19
A3	86.41	5.5	39	24
A4	82.19	6.5	29	18
A5	94.12	0.5	6	9
A6	40.1	12	13	3
A7	60.55	15	25	21
A8	64.14	13.8	20	27
A9	56.16	16	22	19
A10	60.92	15.8	25	23

>TRINITY_DN43443_c5_g3_i1 from sample A9 / Example spliced isoform

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGG
GAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATGATGAAGCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCATGCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA

>TRINITY_DN43443_c5_g3_i9 from sample A9 / Example unspliced isoform:

CAAGACCGTTGTGATTGAACTTTCTAAATTTGTGATCGAACATTCTAGAAAGGGTAAATCACCACCTTAATAAAAACGTTTTTTCATTCTTTTTGCTCTGGTGAGGTGAGGGATGGCCACGACACCCCAACCAAGAGAAATCGATCTGTCATGGAGCCCAAATTGATCCGTCGCTTCATAGAAAACCTACAATCCCACGG
TCTAGGCACTGGATCCGCCTTGAAGAACCTTCACACACTGGTGGCATAAATCTTGATCTCCAACACGTTATTGATCTAAATTTGTTGATGTATTAGGATAGAAACTGGATGATAATTTTTAGAAATTATTACAGGGAATAAGTGCATGTTGCAGGATTGGAGCATTTAGAGGGCAGTTACCTTCCGCGGACGCAATGATG

ATGAAGgtgaggaaatcatctcatttgcataacaaatttacatatgattgtgttattattcctacatgatgaaggtgaccatatcctgattgggaggacgaagactgtcaaattgctgagaaaagctacgaagaaacactagcaaacggaaatgtcttcatttcaaccctggcgagggagtggtgaaattaagactcaac
caatttttttctattggtttataactctaaaagctcccatgacatgggaaacatagagaaattgacatggcaaaagagtgactcgaatgtgcgtggctgcaatatgtagtgtaggggaaatcaccaccttaataaaaacgttttttcattctttgctttttgctagCTCGGCCGCTCCTCTCTTTGTGTTCGGCTTCCAT
GCTGTGAACGCTCTGGTAGTGAATTCATCCGTAGAAATTGCTTATGAATCCACGGCCAAA