sp|F4JYC8|PTM_ARATH ----> DDT domain-containing protein PTM
Intron 1 GTAAGTTAAA ... ACTGTCACAG
| Sample | Retention score (%) | Exon junction reads | 5'ss reads | 3'ss reads |
| A1 | 18.89 | 146 | 41 | 27 |
| A2 | 20.17 | 95 | 23 | 25 |
| A3 | 20.92 | 121 | 34 | 30 |
| A4 | 24.22 | 97 | 34 | 28 |
| A5 | 22.99 | 72 | 25 | 18 |
| A6 | 21.43 | 11 | 0 | 6 |
| A7 | 12.5 | 21 | 2 | 4 |
| A8 | 33.33 | 3 | 2 | 1 |
| A9 | 17.95 | 16 | 3 | 4 |
| A10 | 14.75 | 26 | 5 | 4 |
>TRINITY_DN126397_c1_g2_i4 from sample A1 / Example spliced isoformTCTTGCTTGATTGATTGTTGAGGCTGGAAAATAAAAATGGAAGGCGATGAATACCTCTACTCAGTTTGCGCTAGTGCCATTTTGACGATCATCCGACTAGGTCGCGAGCGATGGGAGCCAACAGGTTCGAATCCGGGGAGGACATCGTGGGTAAGTCCGTGCAGAAGAAATTCAAGGGTTATGGGGTTTTCACGGGCAAA
GTCGTTTCCTACAACTCGGCCCGGGGGTTTTACAGGGTCAGGTATGAGGACGGGGACGCAGAGGAGCTAGACATGGACGATGTCCGTTGCATTCTGGTGCCAGAAAATGAAAATGGCAGGAAGAGGAAGCGCGACGATCAGACTCCGAAGCGGCGAAAGAGGAGGAGAAAGGATGGCGTCGGCCGGCCCAGAAAGACTAC
AAAGGAGGCTGAGGCTTCGAATAAGGGAGCTAGAGGGAGGAGTAGAAAAAAGGAAACTGAGGTTTCGAACACGGGACTTATTGGGGATAGTGGAGGAAAGGAGGTCGAGGTTTCGAACACGGGACTTATTGGGGATAGTAGAGGAAAGGAGGCAGAGGTTTCGAACACGGAACTAATTGGGGATAGTATAGGAAAGGAGG
CCGAGGTTTCGGACACGGGACCTCCTCTTGGAGGGAGTAGAGGAAAGGAGTTCGAGGATTCGAACACGGGACCTCATCTCGGAGGGAGCAGAGGAAAGGAGGTCAAGGATTCGAACACGGGAGATCTCGTAGGGAGCAGAGAAAAGGAGGCGGAGTTCTCGAACATGGAACCTCGTAAGAGGTTTAGAAGAAAGATGTTA
GAGATTTGGAAGACGGGACCTGTTAGGAGGAGTCCTCGTGTGAGGGAATCAGCGGAGAATGCTATCGAAAAGCCCACTCCGCCTAAGCAGCGCAGGTCCAGGGTTAGGGTTCGACATGACGATTTGGCACGGTCAATGCCCTCGGTGCCCCAACTGCCCCTTTCTTCCATCGATTTATGTGTGCCGGAGGAATCGGTGCC
GAATTTATTATATGTTTACAATTCTTTGAGGTCTTTTAGTAAATTGTTGTTTTTGAGTCCGTTCGGGCTTGATGATTTCGTTGGGGCATTGAACTGCAAGTCTGCTAATTCTTTACTAGATGCCGTGCACGTCAAGCTTCTGCAAGCGTTGAGGAGGCATCTTTATTCTCTTTCCAAAGAAGAGTGTTCTGCTGCTGCAG
ATGCGGCTAGATGTTTGAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGAT
GAGATCATGGAAGCCGAAGAAATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCAT
TTCAAGTCAAATCCCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGAT
GTGTTGGTGTTTCAAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTGTCAGATTCTTCCTTTGATCGAACTACA
ACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATG
CTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAG
CAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCT
GGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAA
CATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGA
ATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTACATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCA
GCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTACAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTC
AGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCAAGGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGCATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAGATAAGGAATCTTG
ATGCTTGCATTAGATGGGATGATCTTCTAAATTCAGAAGATTTCTGTCAACCTAAAATGGTAGGAGACAAAGAGTCTGCAGTCTTCAAGAAGGCTGTTATTTGTGCCAAGAATTCAGAAAATGGTCTGATAAAGTACTTGATAGACTTTGGGAAACAGAAAGCCATTCCAACCTGCATTGTTAAATATGGAACAAAGCTT
GAAGAGGCTTTAGATGGTACATGCAAGTATTGGCTAAGCGAGTCTCATGTGCCTCTGTGCTTGCTGAAGGATTATGAAGAGGAAGTGCGATTACATGCCCACCCAAACAAAGGTTCAAAGAGATTCCGTAAGGTTCATTATAGACTCTCAAAAACTTCCAAGTTGGACGTATTTACCATCCTCTTGGCAAAGGCAGAGAA
TCGTGAGAAATGTTCATGTACCCATTGCAACAAGGATGTACCAATCCGGAAAGCAGTTCGATGCCATTTTTGTCAAGGTTCATTTCATCGGAAGTGTTCGATATCTTCCCATTTCTTGAATAAACTGCAGTACACATTTACCTGTTACAAGTGCCACAGTGTTAAGCTGCTAAAGAGCAAGCCTGAAATTAGAGTGCAAT
CAGGGAAGAAGATTTTGGTGCAATCAGGGAAGAAGATTTTGTCAACATGCAAAAGGCGCAGGCTTTCAAAAAAAGTAGAGGAAATTGGGATGTCTACACGAGATGTAAAGCTAAAGAAACCTAAAAGGCATAGGTTGCATAATGATAATGTTGCAATAAAAAAGCATGACAGTGTTGGGAAGGTCACAGTTCGGAGAAAG
AAAATCAAAGGAGATGTTCTTTCGGATGGTTTTTTATGGAAGAAAAATGCGACCGATGAATTAAGAAAGGATTCTAGGCAACGCATGCTTCTCCTTCCAGGCCAGAATGGCATGGATGCCTCTCTATGTCCTAAATGTATTCTTTGTGGCGAGGCATACGACCCTCAATTTTTATATGTTGGATGTGAATATTGTAAAGA
TTGGTTTCATGGAGATGCTTTTGGGCTTACCGTGGAGAATATCATTGATGTGGTTGCTTTCAAGTGTTACAAATGCTGCAAGAATATTGCACCCATCTGTCCTTGTGCCAAGAAGGATAATCTGTGTGATAAAAGGCTTGGTTTGAAGATTACAACGGATAATGAAGTGCTGGATGTAGCCAAAAATGGCAAGCCCTTAG
ATGTGATATACAGCAGATCTAAACTTGTTTCTGATGAGGAGCTTCATGCTAAGTTAGCCAGGGAAGAAGAGCATATATTGACTCAAGTAGAATATGATTCAGAGAATCGTCCTCAAGATCCTAAAGTAGGGAACAATAGGCCAGTTCCAGAAGCAGAAGAGATTCCAAATTCAGCATGCGTTATAGTCAGTGAACAGTTG
CTAACCCCCAAAATAGACCGTGACCCATCTGTTAACAATTATCTGCAGGAAGAAATGGTGAATTGCCAGCAATATGAGCAAGATGACAGGTCAAGGGAGGAAACTGTTGGATCTGAAGTACATAAAACAGAGCCCATCCAGATTTATCCTGATACACAACCCCAATCCATGCTTTCCTGTACTGAATTATTGGCTTCTGA
GGATGATCAAGTAGATCTTATTGATCCATCACCAAATGTTGCTGGAAATTATTCTGAAAATTTACAGCCTGTTGACAAGGGAGAATATGCATTTTCTCAACAATTAAATGTTCAAAATTGTTCCGAAAATTTACTGCCTGTCGAGGAGGTAAATGCATTTTCTCAACTACCAAATGTTCAAAATTGTTCTGAAAATTTAC
TGCCTTTTGAGGGAGAAGATACATTTTCTCAACTACCAAATGTTCAAAATTGTTCTGAAACTTTTCTGCTTGTTGAGGAGGGAGAAGTTGCATTTTCTCAACTACCAATTGTTCAAAATTGTTCTGAAACTTTAGTGCCTGCTGACGAGGAAGAAAAGACATTATCTCAACTACAGGGCATAAATGATGGTGTTCATCAT
TCCTCAGGTGATGTTATTTCACCAGACCTTTCAGAAAACAAAGGGTGCCATCTAGCAAATAGATTTTCATTGGAAAATTTCATAAATCAACCATATAGAATAGGTGGTGAGATTACTTCAGAAGAGATGGAAAAGAAGATATGTTCAACGTGCAACTCAATAGAGGTGCCTCCTGATCTTCTGTGCAGTGGATGTGGCCT
TGCCATTCATTGCCATTGTTATGAACAAGCACCCACTGGCCTTTGGACTGCATCTGGATGGATTTGTGGTTGTTGTCAGGATGGTTTTGTACCAGGCTAAGGCATCCTTTGGGGAATGCCAGAAGGCTCTGGTTAGATCAATACTTTTTGCCCAACAAAGGCCTGATCCCATATTATGATGATCAAGTCGACGAGGACTT
TCATATATGAACGGGAATCGATCTTCTCTATTATTCTCCATGAACCTTTCCAGTAGCTTCCTTCTGCAAGGGTGAAACAATGGGAGCAATGGTGTTCCGACTTGTTCATCCCTGATTAGAAGACTACAATTGTGGAACTATTTTCTTGGATACCATTTGTAAATTGTTATTGTTTTGCAGCATCCACTAGCTTTTCCTGT
CAAGGTGGAATTCACTGAGATAGAACAAACACTCGAATAGAACGAAAAAAATGAAAGTGCCTGGTGTTTGTGTATATGTTTTTGTAACACCCAGATTTCTCTGGCATTACAAAAGTGGACGACTGTCTCATTAAAACTGTAAGTGTATCCCATATTCATAAGCTCATTCTTTTTCCATCCATTGTTCCTGATGATCTCTG
CTTTTAAGAGTCAAGCAGTTTGTGTCTTGAACAATCTAAAAAGAGCTATTTATTGTGAAGCTTCCATACATATCTTTAAATAGTGATGACGTAACAGAGCTTTGGCTTCTCTCTGTTAACCATTTTCTTACATTCTCTATACATATCATTAAATAGTGATGACGTAACAGAGCTTTGGCTTCTCTCTGTTAACCATTTTC
TTACATTCTCTATACATATCATTAAATAGTGATGACGTAACAGA
>TRINITY_DN126397_c1_g2_i8 from sample A1 / Example unspliced isoform:AGAAATTGGGCATATGGCAGACTGGATCTACAAGGTTTAGTAATATGATTATTGTCAATGACTTCCACAGTTTGAACTGGTACAAAGTCCTTGCTTTTAGCAAGCTGTCTGGTTCTGTTGATATCAATTGATTTAGATCTGCTAACTGCCTCTTAGCTGAGGAATAGATATTCACATTCTTTGTAATAAATGCTATTATG
AGAAACTTAGATAATCGATCACATGTTGGGTGAGCTATTATTTGTGAGGATTATCACAATGTGGGAGTTTTAGCCCTCCACATGAAGTTCTGTGATTAAGAAGTTGCTTATGGGTTTAATGAATAAACTAGGAGTTCCAAATGGAAATAATTCAACATGGAGGTCATCTTTCTATAGGAGGAAGCACTAGAAACAAAAAA
ATTGCAATTGCCTCTGTCCAGCGACTGCTATTCTCATGGACTGACTGCTTTTCTGCATGTGTTTGTGGCCCTTAATTACTATAATGAAATAAGCTATGCCCTTATTAAGCCCACCCATTTGTCTGTCACTCTGCATTATAGGAAGTCTTACTGGTTTGCTCCATATGCACCTTTACCCTTGCAGTGTTTTCAACCATGGT
TGTCTGATTAGGCTACTGTCCTTATGACTCTGCTAAATCCCCACTATAACCATGAATAGGAAATAGACATCCAGGACTTTTGTCCCTATTCTTATCTCTAAATTGTTTGATCCTTTGAGGTATTTTTTATTGCTTTGTTTCAATATTCATATTTCCAAGCTTGTTGAATTTATAATGTTTAATTAATCTGCATTTTCACT
GTGTAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGATGAGATCATGGAAG
CCGAAGAAATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCATTTCAAGTCAAATC
CCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGATGTGTTGGTGTTTC
AAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTgtaagttaaacttctgatggcttgaattactggtcttctgt
tactttttctgtatgcatatttaactgaggttctttatgattataatgccgttatattattagactgcttttaccgaagaaactaattgtataaaaactaaaaagacatcattcatgcatggtccattgatcaatttcacgctttttttgatatcgtgtttgggaatctctgcttattcagatttgtttcaaggtccttg
tcatttgatggaatctttgttatttcttgcattaaaaagcttttgaaatttcaaaactatttattttttccctcccaattacattgtttgcaaatgccaattatatatctttccctttatcaggactttcgagacatttcaatattccacattcgtagcaatcttctggttctgaatggaagctttcaatggagccaata
atttatagcttgccaagctggatagtttcattccttaaattgtatgcactttcttttggtttgatagagctttatattttcgttttagtatagttctaaggatggatgttttacttcaagatgccttttggtcacatcatgtttggagcgagtcgtaggcttgtcacattttaggagaagaatctttataccattatttt
ccattgttaatgatgcataagaaagtagattcagagttacaatgattttgagaccaatttgtattggattttatttaattcaaagcatgtctttcaactatactgtcacagGTCAGATTCTTCCTTTGATCGAACTACAACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAG
CTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATGCTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAG
GGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAGCAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGG
TTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCTGGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCAT
ATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAACATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAA
GTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGAATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTACA
TGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCAGCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTA
CAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTCAGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCA
Intron 2 GTAAGAATTT ... TTATTGGCAG
| Sample | Retention score (%) | Exon junction reads | 5'ss reads | 3'ss reads |
| A1 | 21.3 | 109 | 36 | 23 |
| A2 | 22.4 | 97 | 23 | 33 |
| A3 | 20.41 | 78 | 18 | 22 |
| A4 | 22.12 | 81 | 20 | 26 |
| A5 | 26.44 | 32 | 10 | 13 |
| A6 | 14.29 | 6 | 1 | 1 |
| A7 | 23.71 | 37 | 12 | 11 |
| A8 | 24.44 | 17 | 4 | 7 |
| A9 | 30 | 7 | 3 | 3 |
| A10 | 21.31 | 24 | 7 | 6 |
>TRINITY_DN109106_c0_g1_i4 from sample A5 / Example spliced isoformATACTCTTGCTTGATTGATTGTTGAGGCTGGAAAATAAAAATGGAAGGCGATGAATACCTCTACTCAGTTTGCGCTAGTGCCATTTTGACGATCATCCGACTAGGTCGCGAGCGATGGGAGCCAACAGGTTCGAATCCGGGGAGGACATCGTGGGTAAGTCCGTGCAGAAGAAATTCAAGGGTTATGGGGTTTTCACGGG
CAAAGTCGTTTCCTACAACTCGGCCCGGGGGTTTTACAGGGTCAGGTATGAGGACGGGGACGCAGAGGAGCTAGACATGGACGATGTCCGTTGCATTCTGGTGCCAGAAAATGAAAATGGCAGGAAGAGGAAGCGCGACGATCAGACTCCGAAGCGGCGAAAGAGGAGGAGAAAGGATGGCGTCGGCCGGCCCAGAAAGA
CTACAAAGGAGGCTGAGGCTTCGAATAAGGGAGCTAGAGGGAGGAGTAGAAAAAAGGAAACTGAGGTTTCGAACACGGGACTTATTGGGGATAGTGGAGGAAAGGAGGTCGAGGTTTCGAACACGGGACTTATTGGGGATAGTGGAGGAAAGGAGGTCGAGGTTTCGAACACGGGACTTATTGGGGATAGTAGAGGAAAG
GAGGCAGAGGTTTCGAACACGGAACTAATTGGGGATAGTATAGGAAAGGAGGCCGAGGTTTCGGACACGGGACCTGCTCTTGGAGGGAGTAGAGGAAAGGAGTTCGAGGATTCGAACACGGGACCTCATCTCGGAGGGAGCAGAGGAAAGGAGGTCAAGGATTCGAACACGGGAGATCTCGTAGGGAGCAGAGAAAAGGA
GGCGGAGTTCTCGAACATGGAACCTCGTAAGAGGTTTAGAAGAAAGATGTTAGAGATTTGGAAGACGGGACCTGTTAGGAGGAGTCCTCGTGTGAGGGAATCAGCGGAGAATGCTATCGAAAAGCCCACTCCGCCTAAGCAGCGCAGGTCCAGGGTTAGGGTTCGACATGACGATTTGGCACGGTCAATGCCCTCGGTGC
CCCAACTGCCCCTTTCTTCCATCGATTTATGTGTGCCGGAGGAATCGGTGCCGAATTTATTATATGTTTACAATTCTTTGAGGTCTTTTAGTAAATTGTTGTTTTTGAGTCCGTTCGGGCTTGATGATTTCGTTGGGGCATTGAACTGCAAGTCTGCTAATTCTTTACTAGATGCCGTGCACGTCAAGCTTCTGCAAGCG
TTGAGGAGGCATCTTTATTCTCTTTCCAAAGAAGAGTGTTCTGCTGCTGCAGATGCGGCTAGATGTTTGAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTA
CTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGATGAGATCATGGAAGCCGAAGAGATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGA
AAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCATTTCAAGTCAAATCCCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATG
GATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGATGTGTTGGTGTTTCAAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTT
CTCTGCCTGCGGTTACTTGTTAGTGTCAGATTCTTCCTTTGATCGAACTACAACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGG
GCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATGCTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAA
GAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAGCAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGC
TGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCTGGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGTTGCTCAACTTCGACGAGTCAATG
TACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAACATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGA
AATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGAATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTATATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGT
CAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCAGCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTACAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTT
CAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTCAGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCAAGGTTTTTTTTTCATTCTTTTCTGAGTTCCTTATTGTCACTATATCTTTGTTCATTTTGGTTTAGTTTGACTGAGGTTTTTAAATGCA
C
>TRINITY_DN109106_c0_g1_i3 from sample A5 / Example unspliced isoform:CGAGGTTTCGAACACGGGACTTATTGGGGATAGTAGAGGAAAGGAGGCAGAGGTTTCGAACACGGAACTAATTGGGGATAGTATAGGAAAGGAGGCCGAGGTTTCGGACACGGGACCTGCTCTTGGAGGGAGTAGAGGAAAGGAGTTCGAGGATTCGAACACGGGACCTCATCTCGGAGGGAGCAGAGGAAAGGAGGTCA
AGGATTCGAACACGGGAGATCTCGTAGGGAGCAGAGAAAAGGAGGCGGAGTTCTCGAACATGGAACCTCGTAAGAGGTTTAGAAGAAAGATGTTAGAGATTTGGAAGACGGGACCTGTTAGGAGGAGTCCTCGTGTGAGGGAATCAGCGGAGAATGCTATCGAAAAGCCCACTCCGCCTAAGCAGCGCAGGTCCAGGGTT
AGGGTTCGACATGACGATTTGGCACGGTCAATGCCCTCGGTGCCCCAACTGCCCCTTTCTTCCATCGATTTATGTGTGCCGGAGGAATCGGTGCCGAATTTATTATATGTTTACAATTCTTTGAGGTCTTTTAGTAAATTGTTGTTTTTGAGTCCGTTCGGGCTTGATGATTTCGTTGGGGCATTGAACTGCAAGTCTGC
TAATTCTTTACTAGATGCCGTGCACGTCAAGCTTCTGCAAGCGTTGAGGAGGCATCTTTATTCTCTTTCCAAAGAAGAGTGTTCTGCTGCTGCAGATGCGGCTAGATGTTTGAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCT
ATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGATGAGATCATGGAAGCCGAAGAGATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTT
GAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCATTTCAAGTCAAATCCCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACC
TGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGATGTGTTGGTGTTTCAAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAG
GAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTGTCAGATTCTTCCTTTGATCGAACTACAACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGG
GATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATGCTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCG
AAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAGCAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCAT
TACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCTGGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCT
GCAAATTTAGCAGTCAAAGTTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAACATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATC
TCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGAATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTATATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAAT
ATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGgtaagaatttatgagattgatttttttgcagtgtagattgttcgagaatttgcattctgaaactcctagaatcaaacacccaattcatttccttaagctggtttggtttcattctttgtgcaacgtcttctttcttaa
catgtgaacagtataacaattgtaaatatctgaattatttcttattcatgacatcgtatggtgattttggttagctgaattattttgcccttttgttattggcagCTAGAGACGAATATCAGACTTGTTGCACTTTCAGCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTAC
AACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTCAGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCAAG
GTTTTTTTTTCATTCTTTTCTGAGTTCCTTATTGTCACTATATCTTTGTTCATTTTGGTTTAGTTTGACTGAGGTTTTTAAATGCAC
Intron 3 GTATGCACTT ... ACTGTCACAG
| Sample | Retention score (%) | Exon junction reads | 5'ss reads | 3'ss reads |
| A1 | 40.91 | 39 | 13 | 27 |
| A2 | 47.17 | 28 | 0 | 25 |
| A3 | 43.48 | 39 | 4 | 30 |
| A4 | 46.67 | 32 | 9 | 28 |
| A5 | 50 | 18 | 0 | 18 |
| A6 | 50 | 6 | 0 | 6 |
| A7 | 50 | 4 | 0 | 4 |
| A8 | 50 | 1 | 0 | 1 |
| A9 | 50 | 4 | 0 | 4 |
| A10 | 50 | 4 | 0 | 4 |
>TRINITY_DN126397_c1_g2_i5 from sample A1 / Example spliced isoformCTGCCTGCGGTTACTTGTTAGTGACTTTCGAGACATTTCAATATTCCACATTCGTAGCAATCTTCTGGTTCTGAATGGAAGCTTTCAATGGAGCCAATAATTTATAGCTTGCCAAGCTGGATAGTTTCATTCCTTAAATTGTCAGATTCTTCCTTTGATCGAACTACAACTCACAAGTATTACAACAGAAATGATACATT
GAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATGCTCATCAGAATTATCTGCTATATCTGTCTCGC
AGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAGCAAGTTCTGAAGGTTCTTTGGAAATAACATCA
TTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCTGGCCGAAAGCATTTTAATAGCAATCCTTCAAA
TAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAACATATTTTCCAGGGAAGCTGTGCAATTCTGCT
GGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGAATGGTGATGGTCATTTTCCAGCCATTGTAGCA
TATATACTGTACATGGAGGGCCAATTACATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCAGCAGCTTGGACTAAGCCTGTTGACGATTCCTC
CACTGTGGTCTCTGGCAGTCGAAATGGTACAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTCAGACCAAGCACTTTACTGGAAGGTCTTACCTT
GCAGTATAGCTCGGCGAGCTGGACGCCAAGGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGCATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAG
>TRINITY_DN126397_c1_g2_i8 from sample A1 / Example unspliced isoform:AGAAATTGGGCATATGGCAGACTGGATCTACAAGGTTTAGTAATATGATTATTGTCAATGACTTCCACAGTTTGAACTGGTACAAAGTCCTTGCTTTTAGCAAGCTGTCTGGTTCTGTTGATATCAATTGATTTAGATCTGCTAACTGCCTCTTAGCTGAGGAATAGATATTCACATTCTTTGTAATAAATGCTATTATG
AGAAACTTAGATAATCGATCACATGTTGGGTGAGCTATTATTTGTGAGGATTATCACAATGTGGGAGTTTTAGCCCTCCACATGAAGTTCTGTGATTAAGAAGTTGCTTATGGGTTTAATGAATAAACTAGGAGTTCCAAATGGAAATAATTCAACATGGAGGTCATCTTTCTATAGGAGGAAGCACTAGAAACAAAAAA
ATTGCAATTGCCTCTGTCCAGCGACTGCTATTCTCATGGACTGACTGCTTTTCTGCATGTGTTTGTGGCCCTTAATTACTATAATGAAATAAGCTATGCCCTTATTAAGCCCACCCATTTGTCTGTCACTCTGCATTATAGGAAGTCTTACTGGTTTGCTCCATATGCACCTTTACCCTTGCAGTGTTTTCAACCATGGT
TGTCTGATTAGGCTACTGTCCTTATGACTCTGCTAAATCCCCACTATAACCATGAATAGGAAATAGACATCCAGGACTTTTGTCCCTATTCTTATCTCTAAATTGTTTGATCCTTTGAGGTATTTTTTATTGCTTTGTTTCAATATTCATATTTCCAAGCTTGTTGAATTTATAATGTTTAATTAATCTGCATTTTCACT
GTGTAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGATGAGATCATGGAAG
CCGAAGAAATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCATTTCAAGTCAAATC
CCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGATGTGTTGGTGTTTC
AAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTGTAAGTTAAACTTCTGATGGCTTGAATTACTGGTCTTCTGT
TACTTTTTCTGTATGCATATTTAACTGAGGTTCTTTATGATTATAATGCCGTTATATTATTAGACTGCTTTTACCGAAGAAACTAATTGTATAAAAACTAAAAAGACATCATTCATGCATGGTCCATTGATCAATTTCACGCTTTTTTTGATATCGTGTTTGGGAATCTCTGCTTATTCAGATTTGTTTCAAGGTCCTTG
TCATTTGATGGAATCTTTGTTATTTCTTGCATTAAAAAGCTTTTGAAATTTCAAAACTATTTATTTTTTCCCTCCCAATTACATTGTTTGCAAATGCCAATTATATATCTTTCCCTTTATCAGGACTTTCGAGACATTTCAATATTCCACATTCGTAGCAATCTTCTGGTTCTGAATGGAAGCTTTCAATGGAGCCAATA
ATTTATAGCTTGCCAAGCTGGATAGTTTCATTCCTTAAATTgtatgcactttcttttggtttgatagagctttatattttcgttttagtatagttctaaggatggatgttttacttcaagatgccttttggtcacatcatgtttggagcgagtcgtaggcttgtcacattttaggagaagaatctttataccattatttt
ccattgttaatgatgcataagaaagtagattcagagttacaatgattttgagaccaatttgtattggattttatttaattcaaagcatgtctttcaactatactgtcacagGTCAGATTCTTCCTTTGATCGAACTACAACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAG
CTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATGCTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAG
GGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAGCAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGG
TTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCTGGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCAT
ATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAACATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAA
GTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGAATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTACA
TGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCAGCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTA
CAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTCAGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCA
Intron 4 GTTTTTTTTT ... AAATTTGCAG
| Sample | Retention score (%) | Exon junction reads | 5'ss reads | 3'ss reads |
| A1 | 13.86 | 155.4 | 1 | 25 |
| A2 | 18.01 | 132 | 0 | 29 |
| A3 | 8 | 115 | 0 | 10 |
| A4 | 15.53 | 136 | 0 | 25 |
| A5 | 11.29 | 55 | 0 | 7 |
| A6 | 0 | 15 | 0 | 0 |
| A7 | 9.09 | 40 | 0 | 4 |
| A8 | 13.33 | 13 | 0 | 2 |
| A9 | 13.33 | 39 | 0 | 6 |
| A10 | 12.08 | 72.8 | 0 | 10 |
>TRINITY_DN126397_c1_g2_i4 from sample A1 / Example spliced isoformTCTTGCTTGATTGATTGTTGAGGCTGGAAAATAAAAATGGAAGGCGATGAATACCTCTACTCAGTTTGCGCTAGTGCCATTTTGACGATCATCCGACTAGGTCGCGAGCGATGGGAGCCAACAGGTTCGAATCCGGGGAGGACATCGTGGGTAAGTCCGTGCAGAAGAAATTCAAGGGTTATGGGGTTTTCACGGGCAAA
GTCGTTTCCTACAACTCGGCCCGGGGGTTTTACAGGGTCAGGTATGAGGACGGGGACGCAGAGGAGCTAGACATGGACGATGTCCGTTGCATTCTGGTGCCAGAAAATGAAAATGGCAGGAAGAGGAAGCGCGACGATCAGACTCCGAAGCGGCGAAAGAGGAGGAGAAAGGATGGCGTCGGCCGGCCCAGAAAGACTAC
AAAGGAGGCTGAGGCTTCGAATAAGGGAGCTAGAGGGAGGAGTAGAAAAAAGGAAACTGAGGTTTCGAACACGGGACTTATTGGGGATAGTGGAGGAAAGGAGGTCGAGGTTTCGAACACGGGACTTATTGGGGATAGTAGAGGAAAGGAGGCAGAGGTTTCGAACACGGAACTAATTGGGGATAGTATAGGAAAGGAGG
CCGAGGTTTCGGACACGGGACCTCCTCTTGGAGGGAGTAGAGGAAAGGAGTTCGAGGATTCGAACACGGGACCTCATCTCGGAGGGAGCAGAGGAAAGGAGGTCAAGGATTCGAACACGGGAGATCTCGTAGGGAGCAGAGAAAAGGAGGCGGAGTTCTCGAACATGGAACCTCGTAAGAGGTTTAGAAGAAAGATGTTA
GAGATTTGGAAGACGGGACCTGTTAGGAGGAGTCCTCGTGTGAGGGAATCAGCGGAGAATGCTATCGAAAAGCCCACTCCGCCTAAGCAGCGCAGGTCCAGGGTTAGGGTTCGACATGACGATTTGGCACGGTCAATGCCCTCGGTGCCCCAACTGCCCCTTTCTTCCATCGATTTATGTGTGCCGGAGGAATCGGTGCC
GAATTTATTATATGTTTACAATTCTTTGAGGTCTTTTAGTAAATTGTTGTTTTTGAGTCCGTTCGGGCTTGATGATTTCGTTGGGGCATTGAACTGCAAGTCTGCTAATTCTTTACTAGATGCCGTGCACGTCAAGCTTCTGCAAGCGTTGAGGAGGCATCTTTATTCTCTTTCCAAAGAAGAGTGTTCTGCTGCTGCAG
ATGCGGCTAGATGTTTGAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGAT
GAGATCATGGAAGCCGAAGAAATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCAT
TTCAAGTCAAATCCCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGAT
GTGTTGGTGTTTCAAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTGTCAGATTCTTCCTTTGATCGAACTACA
ACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATG
CTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAG
CAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCT
GGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAA
CATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGA
ATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTACATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCA
GCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTACAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTC
AGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCAAGGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGCATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAGATAAGGAATCTTG
ATGCTTGCATTAGATGGGATGATCTTCTAAATTCAGAAGATTTCTGTCAACCTAAAATGGTAGGAGACAAAGAGTCTGCAGTCTTCAAGAAGGCTGTTATTTGTGCCAAGAATTCAGAAAATGGTCTGATAAAGTACTTGATAGACTTTGGGAAACAGAAAGCCATTCCAACCTGCATTGTTAAATATGGAACAAAGCTT
GAAGAGGCTTTAGATGGTACATGCAAGTATTGGCTAAGCGAGTCTCATGTGCCTCTGTGCTTGCTGAAGGATTATGAAGAGGAAGTGCGATTACATGCCCACCCAAACAAAGGTTCAAAGAGATTCCGTAAGGTTCATTATAGACTCTCAAAAACTTCCAAGTTGGACGTATTTACCATCCTCTTGGCAAAGGCAGAGAA
TCGTGAGAAATGTTCATGTACCCATTGCAACAAGGATGTACCAATCCGGAAAGCAGTTCGATGCCATTTTTGTCAAGGTTCATTTCATCGGAAGTGTTCGATATCTTCCCATTTCTTGAATAAACTGCAGTACACATTTACCTGTTACAAGTGCCACAGTGTTAAGCTGCTAAAGAGCAAGCCTGAAATTAGAGTGCAAT
CAGGGAAGAAGATTTTGGTGCAATCAGGGAAGAAGATTTTGTCAACATGCAAAAGGCGCAGGCTTTCAAAAAAAGTAGAGGAAATTGGGATGTCTACACGAGATGTAAAGCTAAAGAAACCTAAAAGGCATAGGTTGCATAATGATAATGTTGCAATAAAAAAGCATGACAGTGTTGGGAAGGTCACAGTTCGGAGAAAG
AAAATCAAAGGAGATGTTCTTTCGGATGGTTTTTTATGGAAGAAAAATGCGACCGATGAATTAAGAAAGGATTCTAGGCAACGCATGCTTCTCCTTCCAGGCCAGAATGGCATGGATGCCTCTCTATGTCCTAAATGTATTCTTTGTGGCGAGGCATACGACCCTCAATTTTTATATGTTGGATGTGAATATTGTAAAGA
TTGGTTTCATGGAGATGCTTTTGGGCTTACCGTGGAGAATATCATTGATGTGGTTGCTTTCAAGTGTTACAAATGCTGCAAGAATATTGCACCCATCTGTCCTTGTGCCAAGAAGGATAATCTGTGTGATAAAAGGCTTGGTTTGAAGATTACAACGGATAATGAAGTGCTGGATGTAGCCAAAAATGGCAAGCCCTTAG
ATGTGATATACAGCAGATCTAAACTTGTTTCTGATGAGGAGCTTCATGCTAAGTTAGCCAGGGAAGAAGAGCATATATTGACTCAAGTAGAATATGATTCAGAGAATCGTCCTCAAGATCCTAAAGTAGGGAACAATAGGCCAGTTCCAGAAGCAGAAGAGATTCCAAATTCAGCATGCGTTATAGTCAGTGAACAGTTG
CTAACCCCCAAAATAGACCGTGACCCATCTGTTAACAATTATCTGCAGGAAGAAATGGTGAATTGCCAGCAATATGAGCAAGATGACAGGTCAAGGGAGGAAACTGTTGGATCTGAAGTACATAAAACAGAGCCCATCCAGATTTATCCTGATACACAACCCCAATCCATGCTTTCCTGTACTGAATTATTGGCTTCTGA
GGATGATCAAGTAGATCTTATTGATCCATCACCAAATGTTGCTGGAAATTATTCTGAAAATTTACAGCCTGTTGACAAGGGAGAATATGCATTTTCTCAACAATTAAATGTTCAAAATTGTTCCGAAAATTTACTGCCTGTCGAGGAGGTAAATGCATTTTCTCAACTACCAAATGTTCAAAATTGTTCTGAAAATTTAC
TGCCTTTTGAGGGAGAAGATACATTTTCTCAACTACCAAATGTTCAAAATTGTTCTGAAACTTTTCTGCTTGTTGAGGAGGGAGAAGTTGCATTTTCTCAACTACCAATTGTTCAAAATTGTTCTGAAACTTTAGTGCCTGCTGACGAGGAAGAAAAGACATTATCTCAACTACAGGGCATAAATGATGGTGTTCATCAT
TCCTCAGGTGATGTTATTTCACCAGACCTTTCAGAAAACAAAGGGTGCCATCTAGCAAATAGATTTTCATTGGAAAATTTCATAAATCAACCATATAGAATAGGTGGTGAGATTACTTCAGAAGAGATGGAAAAGAAGATATGTTCAACGTGCAACTCAATAGAGGTGCCTCCTGATCTTCTGTGCAGTGGATGTGGCCT
TGCCATTCATTGCCATTGTTATGAACAAGCACCCACTGGCCTTTGGACTGCATCTGGATGGATTTGTGGTTGTTGTCAGGATGGTTTTGTACCAGGCTAAGGCATCCTTTGGGGAATGCCAGAAGGCTCTGGTTAGATCAATACTTTTTGCCCAACAAAGGCCTGATCCCATATTATGATGATCAAGTCGACGAGGACTT
TCATATATGAACGGGAATCGATCTTCTCTATTATTCTCCATGAACCTTTCCAGTAGCTTCCTTCTGCAAGGGTGAAACAATGGGAGCAATGGTGTTCCGACTTGTTCATCCCTGATTAGAAGACTACAATTGTGGAACTATTTTCTTGGATACCATTTGTAAATTGTTATTGTTTTGCAGCATCCACTAGCTTTTCCTGT
CAAGGTGGAATTCACTGAGATAGAACAAACACTCGAATAGAACGAAAAAAATGAAAGTGCCTGGTGTTTGTGTATATGTTTTTGTAACACCCAGATTTCTCTGGCATTACAAAAGTGGACGACTGTCTCATTAAAACTGTAAGTGTATCCCATATTCATAAGCTCATTCTTTTTCCATCCATTGTTCCTGATGATCTCTG
CTTTTAAGAGTCAAGCAGTTTGTGTCTTGAACAATCTAAAAAGAGCTATTTATTGTGAAGCTTCCATACATATCTTTAAATAGTGATGACGTAACAGAGCTTTGGCTTCTCTCTGTTAACCATTTTCTTACATTCTCTATACATATCATTAAATAGTGATGACGTAACAGAGCTTTGGCTTCTCTCTGTTAACCATTTTC
TTACATTCTCTATACATATCATTAAATAGTGATGACGTAACAGA
>TRINITY_DN126397_c1_g2_i6 from sample A1 / Example unspliced isoform:CTCGGCGAGCTGGACGCCAAGgtttttttttcattcttttctgagttccttattgtcactatatctttgttcattttggtttagtttgactgaggtttttaaatgcacacatattataaatttgcagGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGC
ATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAGGTCTGCAGTCAGTTTGCAATTCCATTATTTTTCTGAGGTTTGAAGCAATTGGCCTTATATTATTCTTATATTCTTAAAATGCCTTACAGATAAGGAATCTTGATGCTTGCATTAGATGGGATGATCTTCTAAATTCAGAAGATTTCTG
TCAACCTAAAATGGTAGGAGACAAAGAGTCTGCAGTCTTCAAGAAGGCTGTTATTTGTGCCAAGAATTCAGAAAATGGTCTGATAAAGTACTTGATAGACTTTGGGAAACAGAAAGCCATTCCAACCTGCATTGTTAAATATGGAACAAAGCTTGAAGAGGCTTTAGATGGTACATGCAAGTATTGGCTAAGCGAGTCTC
ATGTGCCTCTGTGCTTGCTGAAGGATTATGAAGAGGAAGTGCGATTACATGCCCACCCAAACAAAGGTTCAAAGAGATTCCGTAAGGTTCATTATAGACTCTCAAAAACTTCCAAGTTGGACGTATTTACCATCCTCTTGGCAAAGGCAGAGAATCGTGAGAAATGTTCATGTACCCATTGCAACAAGGATGTACCAATC
CGG
Intron 5 GTATGATGTT ... TTGTTTGTAG
| Sample | Retention score (%) | Exon junction reads | 5'ss reads | 3'ss reads |
| A1 | 29.25 | 75 | 31 | 28.4 |
| A2 | 22.5 | 62 | 18 | 12 |
| A3 | 15.79 | 64 | 12 | 22.4 |
| A4 | 29.03 | 66 | 27 | 15.4 |
| A5 | 8.33 | 11 | 1 | 3.4 |
| A6 | 27.14 | 51 | 19 | 18.8 |
| A7 | 22.05 | 99 | 28 | 35.4 |
| A8 | 33.33 | 48 | 24 | 16.2 |
| A9 | 18.82 | 69 | 16 | 34.4 |
| A10 | 23.81 | 80 | 25 | 24.8 |
>TRINITY_DN126397_c1_g2_i4 from sample A1 / Example spliced isoformTCTTGCTTGATTGATTGTTGAGGCTGGAAAATAAAAATGGAAGGCGATGAATACCTCTACTCAGTTTGCGCTAGTGCCATTTTGACGATCATCCGACTAGGTCGCGAGCGATGGGAGCCAACAGGTTCGAATCCGGGGAGGACATCGTGGGTAAGTCCGTGCAGAAGAAATTCAAGGGTTATGGGGTTTTCACGGGCAAA
GTCGTTTCCTACAACTCGGCCCGGGGGTTTTACAGGGTCAGGTATGAGGACGGGGACGCAGAGGAGCTAGACATGGACGATGTCCGTTGCATTCTGGTGCCAGAAAATGAAAATGGCAGGAAGAGGAAGCGCGACGATCAGACTCCGAAGCGGCGAAAGAGGAGGAGAAAGGATGGCGTCGGCCGGCCCAGAAAGACTAC
AAAGGAGGCTGAGGCTTCGAATAAGGGAGCTAGAGGGAGGAGTAGAAAAAAGGAAACTGAGGTTTCGAACACGGGACTTATTGGGGATAGTGGAGGAAAGGAGGTCGAGGTTTCGAACACGGGACTTATTGGGGATAGTAGAGGAAAGGAGGCAGAGGTTTCGAACACGGAACTAATTGGGGATAGTATAGGAAAGGAGG
CCGAGGTTTCGGACACGGGACCTCCTCTTGGAGGGAGTAGAGGAAAGGAGTTCGAGGATTCGAACACGGGACCTCATCTCGGAGGGAGCAGAGGAAAGGAGGTCAAGGATTCGAACACGGGAGATCTCGTAGGGAGCAGAGAAAAGGAGGCGGAGTTCTCGAACATGGAACCTCGTAAGAGGTTTAGAAGAAAGATGTTA
GAGATTTGGAAGACGGGACCTGTTAGGAGGAGTCCTCGTGTGAGGGAATCAGCGGAGAATGCTATCGAAAAGCCCACTCCGCCTAAGCAGCGCAGGTCCAGGGTTAGGGTTCGACATGACGATTTGGCACGGTCAATGCCCTCGGTGCCCCAACTGCCCCTTTCTTCCATCGATTTATGTGTGCCGGAGGAATCGGTGCC
GAATTTATTATATGTTTACAATTCTTTGAGGTCTTTTAGTAAATTGTTGTTTTTGAGTCCGTTCGGGCTTGATGATTTCGTTGGGGCATTGAACTGCAAGTCTGCTAATTCTTTACTAGATGCCGTGCACGTCAAGCTTCTGCAAGCGTTGAGGAGGCATCTTTATTCTCTTTCCAAAGAAGAGTGTTCTGCTGCTGCAG
ATGCGGCTAGATGTTTGAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGAT
GAGATCATGGAAGCCGAAGAAATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCAT
TTCAAGTCAAATCCCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGAT
GTGTTGGTGTTTCAAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTGTCAGATTCTTCCTTTGATCGAACTACA
ACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATG
CTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAG
CAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCT
GGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAA
CATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGA
ATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTACATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCA
GCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTACAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTC
AGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCAAGGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGCATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAGATAAGGAATCTTG
ATGCTTGCATTAGATGGGATGATCTTCTAAATTCAGAAGATTTCTGTCAACCTAAAATGGTAGGAGACAAAGAGTCTGCAGTCTTCAAGAAGGCTGTTATTTGTGCCAAGAATTCAGAAAATGGTCTGATAAAGTACTTGATAGACTTTGGGAAACAGAAAGCCATTCCAACCTGCATTGTTAAATATGGAACAAAGCTT
GAAGAGGCTTTAGATGGTACATGCAAGTATTGGCTAAGCGAGTCTCATGTGCCTCTGTGCTTGCTGAAGGATTATGAAGAGGAAGTGCGATTACATGCCCACCCAAACAAAGGTTCAAAGAGATTCCGTAAGGTTCATTATAGACTCTCAAAAACTTCCAAGTTGGACGTATTTACCATCCTCTTGGCAAAGGCAGAGAA
TCGTGAGAAATGTTCATGTACCCATTGCAACAAGGATGTACCAATCCGGAAAGCAGTTCGATGCCATTTTTGTCAAGGTTCATTTCATCGGAAGTGTTCGATATCTTCCCATTTCTTGAATAAACTGCAGTACACATTTACCTGTTACAAGTGCCACAGTGTTAAGCTGCTAAAGAGCAAGCCTGAAATTAGAGTGCAAT
CAGGGAAGAAGATTTTGGTGCAATCAGGGAAGAAGATTTTGTCAACATGCAAAAGGCGCAGGCTTTCAAAAAAAGTAGAGGAAATTGGGATGTCTACACGAGATGTAAAGCTAAAGAAACCTAAAAGGCATAGGTTGCATAATGATAATGTTGCAATAAAAAAGCATGACAGTGTTGGGAAGGTCACAGTTCGGAGAAAG
AAAATCAAAGGAGATGTTCTTTCGGATGGTTTTTTATGGAAGAAAAATGCGACCGATGAATTAAGAAAGGATTCTAGGCAACGCATGCTTCTCCTTCCAGGCCAGAATGGCATGGATGCCTCTCTATGTCCTAAATGTATTCTTTGTGGCGAGGCATACGACCCTCAATTTTTATATGTTGGATGTGAATATTGTAAAGA
TTGGTTTCATGGAGATGCTTTTGGGCTTACCGTGGAGAATATCATTGATGTGGTTGCTTTCAAGTGTTACAAATGCTGCAAGAATATTGCACCCATCTGTCCTTGTGCCAAGAAGGATAATCTGTGTGATAAAAGGCTTGGTTTGAAGATTACAACGGATAATGAAGTGCTGGATGTAGCCAAAAATGGCAAGCCCTTAG
ATGTGATATACAGCAGATCTAAACTTGTTTCTGATGAGGAGCTTCATGCTAAGTTAGCCAGGGAAGAAGAGCATATATTGACTCAAGTAGAATATGATTCAGAGAATCGTCCTCAAGATCCTAAAGTAGGGAACAATAGGCCAGTTCCAGAAGCAGAAGAGATTCCAAATTCAGCATGCGTTATAGTCAGTGAACAGTTG
CTAACCCCCAAAATAGACCGTGACCCATCTGTTAACAATTATCTGCAGGAAGAAATGGTGAATTGCCAGCAATATGAGCAAGATGACAGGTCAAGGGAGGAAACTGTTGGATCTGAAGTACATAAAACAGAGCCCATCCAGATTTATCCTGATACACAACCCCAATCCATGCTTTCCTGTACTGAATTATTGGCTTCTGA
GGATGATCAAGTAGATCTTATTGATCCATCACCAAATGTTGCTGGAAATTATTCTGAAAATTTACAGCCTGTTGACAAGGGAGAATATGCATTTTCTCAACAATTAAATGTTCAAAATTGTTCCGAAAATTTACTGCCTGTCGAGGAGGTAAATGCATTTTCTCAACTACCAAATGTTCAAAATTGTTCTGAAAATTTAC
TGCCTTTTGAGGGAGAAGATACATTTTCTCAACTACCAAATGTTCAAAATTGTTCTGAAACTTTTCTGCTTGTTGAGGAGGGAGAAGTTGCATTTTCTCAACTACCAATTGTTCAAAATTGTTCTGAAACTTTAGTGCCTGCTGACGAGGAAGAAAAGACATTATCTCAACTACAGGGCATAAATGATGGTGTTCATCAT
TCCTCAGGTGATGTTATTTCACCAGACCTTTCAGAAAACAAAGGGTGCCATCTAGCAAATAGATTTTCATTGGAAAATTTCATAAATCAACCATATAGAATAGGTGGTGAGATTACTTCAGAAGAGATGGAAAAGAAGATATGTTCAACGTGCAACTCAATAGAGGTGCCTCCTGATCTTCTGTGCAGTGGATGTGGCCT
TGCCATTCATTGCCATTGTTATGAACAAGCACCCACTGGCCTTTGGACTGCATCTGGATGGATTTGTGGTTGTTGTCAGGATGGTTTTGTACCAGGCTAAGGCATCCTTTGGGGAATGCCAGAAGGCTCTGGTTAGATCAATACTTTTTGCCCAACAAAGGCCTGATCCCATATTATGATGATCAAGTCGACGAGGACTT
TCATATATGAACGGGAATCGATCTTCTCTATTATTCTCCATGAACCTTTCCAGTAGCTTCCTTCTGCAAGGGTGAAACAATGGGAGCAATGGTGTTCCGACTTGTTCATCCCTGATTAGAAGACTACAATTGTGGAACTATTTTCTTGGATACCATTTGTAAATTGTTATTGTTTTGCAGCATCCACTAGCTTTTCCTGT
CAAGGTGGAATTCACTGAGATAGAACAAACACTCGAATAGAACGAAAAAAATGAAAGTGCCTGGTGTTTGTGTATATGTTTTTGTAACACCCAGATTTCTCTGGCATTACAAAAGTGGACGACTGTCTCATTAAAACTGTAAGTGTATCCCATATTCATAAGCTCATTCTTTTTCCATCCATTGTTCCTGATGATCTCTG
CTTTTAAGAGTCAAGCAGTTTGTGTCTTGAACAATCTAAAAAGAGCTATTTATTGTGAAGCTTCCATACATATCTTTAAATAGTGATGACGTAACAGAGCTTTGGCTTCTCTCTGTTAACCATTTTCTTACATTCTCTATACATATCATTAAATAGTGATGACGTAACAGAGCTTTGGCTTCTCTCTGTTAACCATTTTC
TTACATTCTCTATACATATCATTAAATAGTGATGACGTAACAGA
>TRINITY_DN126397_c1_g2_i1 from sample A1 / Example unspliced isoform:TCTTGCTTGATTGATTGTTGAGGCTGGAAAATAAAAATGGAAGGCGATGAATACCTCTACTCAGTTTGCGCTAGTGCCATTTTGACGATCATCCGACTAGGTCGCGAGCGATGGGAGCCAACAGGTTCGAATCCGGGGAGGACATCGTGGGTAAGTCCGTGCAGAAGAAATTCAAGGGTTATGGGGTTTTCACGGGCAAA
GTCGTTTCCTACAACTCGGCCCGGGGGTTTTACAGGGTCAGGTATGAGGACGGGGACGCAGAGGAGCTAGACATGGACGATGTCCGTTGCATTCTGGTGCCAGAAAATGAAAATGGCAGGAAGAGGAAGCGCGACGATCAGACTCCGAAGCGGCGAAAGAGGAGGAGAAAGGATGGCGTCGGCCGGCCCAGAAAGACTAC
AAAGGAGGCTGAGGCTTCGAATAAGGGAGCTAGAGGGAGGAGTAGAAAAAAGGAAACTGAGGTTTCGAACACGGGACTTATTGGGGATAGTGGAGGAAAGGAGGTCGAGGTTTCGAACACGGGACTTATTGGGGATAGTAGAGGAAAGGAGGCAGAGGTTTCGAACACGGAACTAATTGGGGATAGTATAGGAAAGGAGG
CCGAGGTTTCGGACACGGGACCTCCTCTTGGAGGGAGTAGAGGAAAGGAGTTCGAGGATTCGAACACGGGACCTCATCTCGGAGGGAGCAGAGGAAAGGAGGTCAAGGATTCGAACACGGGAGATCTCGTAGGGAGCAGAGAAAAGGAGGCGGAGTTCTCGAACATGGAACCTCGTAAGAGGTTTAGAAGAAAGATGTTA
GAGATTTGGAAGACGGGACCTGTTAGGAGGAGTCCTCGTGTGAGGGAATCAGCGGAGAATGCTATCGAAAAGCCCACTCCGCCTAAGCAGCGCAGGTCCAGGGTTAGGGTTCGACATGACGATTTGGCACGGTCAATGCCCTCGGTGCCCCAACTGCCCCTTTCTTCCATCGATTTATGTGTGCCGGAGGAATCGGTGCC
GAATTTATTATATGTTTACAATTCTTTGAGGTCTTTTAGTAAATTGTTGTTTTTGAGTCCGTTCGGGCTTGATGATTTCGTTGGGGCATTGAACTGCAAGTCTGCTAATTCTTTACTAGATGCCGTGCACGTCAAGCTTCTGCAAGCGTTGAGGAGGCATCTTTATTCTCTTTCCAAAGAAGAGTGTTCTGCTGCTGCAG
ATGCGGCTAGATGTTTGAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGAT
GAGATCATGGAAGCCGAAGAAATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCAT
TTCAAGTCAAATCCCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGAT
GTGTTGGTGTTTCAAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTGTCAGATTCTTCCTTTGATCGAACTACA
ACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATG
CTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAG
CAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCT
GGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAA
CATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGA
ATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTACATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCA
GCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTACAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTC
AGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCAAGGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGCATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAGATAAGGAATCTTG
ATGCTTGCATTAGATGGGATGATCTTCTAAATTCAGAAGATTTCTGTCAACCTAAAATGGTAGGAGACAAAGAGTCTGCAGTCTTCAAGAAGGCTGTTATTTGTGCCAAGAATTCAGAAAATGGTCTGATAAAGTACTTGATAGACTTTGGGAAACAGAAAGCCATTCCAACCTGCATTGTTAAATATGGAACAAAGCTT
GAAGAGGCTTTAGATGGTACATGCAAGTATTGGCTAAGCGAGTCTCATGTGCCTCTGTGCTTGCTGAAGGATTATGAAGAGGAAGTGCGATTACATGCCCACCCAAACAAAGGTTCAAAGAGATTCCGTAAGGTTCATTATAGACTCTCAAAAACTTCCAAGTTGGACGTATTTACCATCCTCTTGGCAAAGGCAGAGAA
TCGTGAGAAATGTTCATGTACCCATTGCAACAAGGATGTACCAATCCGGAAAGCAGTTCGATGCCATTTTTGTCAAGGTTCATTTCATCGGAAGTGTTCGATATCTTCCCATTTCTTGAATAAACTGCAGTACACATTTACCTGTTACAAGTGCCACAGTGTTAAGCTGCTAAAGAGCAAGCCTGAAATTAGAGTGCAAT
CAGGGAAGAAGATTTTGGTGCAATCAGGGAAGAAGATTTTGTCAACATGCAAAAGGCGCAGGCTTTCAAAAAAAGTAGAGGAAATTGGGATGTCTACACGAGATGTAAAGCTAAAGAAACCTAAAAGGCATAGGTTGCATAATGATAATGTTGCAATAAAAAAGCATGACAGTGTTGGGAAGGTCACAGTTCGGAGAAAG
AAAATCAAAGGAGATGTTCTTTCGGATGGTTTTTTATGGAAGAAAAATGCGACCGATGAATTAAGAAAGGATTCTAGGCAACGCATGCTTCTCCTTCCAGGCCAGAATGGCATGGATGCCTCTCTATGTCCTAAATGTATTCTTTGTGGCGAGGCATACGACCCTCAATTTTTATATGTTGGATGTGAATATTGTAAAGg
tatgatgttgggtggtccacttgattaatgcttttttcttattgatttatcaagtatcattttgaaatgggctatggtgtggagcttttttcagccccaaatggtagtactatacatcatctgtggatagatagaagaggatggtgaaaatgttccgttccaagaagtatttagctaactaggacagccataaatcaaga
ttttacatcataccagtgaaattaattacgtagagtacattaccacatgcatgaccacgttctgactcttaaaatctcactttcaatcttccagttaatttctaacgttttaattttgtttgtagATTGGTTTCATGGAGC
Intron 6 GTAAGTTAAA ... CCTTTATCAG
| Sample | Retention score (%) | Exon junction reads | 5'ss reads | 3'ss reads |
| A1 | 55.92 | 35.4 | 41 | 11 |
| A2 | 56.8 | 21.6 | 23 | 0 |
| A3 | 60.1 | 26 | 34 | 6 |
| A4 | 68 | 17.8 | 34 | 7 |
| A5 | 66.51 | 14.4 | 25 | 0 |
| A6 | 0 | 0.8 | 0 | 0 |
| A7 | 46.66 | 3.2 | 2 | 0 |
| A8 | 73.34 | 0.8 | 2 | 0 |
| A9 | 60 | 2.4 | 3 | 0 |
| A10 | 77.14 | 1.6 | 5 | 1 |
>TRINITY_DN126397_c1_g2_i5 from sample A1 / Example spliced isoformCTGCCTGCGGTTACTTGTTAGTGACTTTCGAGACATTTCAATATTCCACATTCGTAGCAATCTTCTGGTTCTGAATGGAAGCTTTCAATGGAGCCAATAATTTATAGCTTGCCAAGCTGGATAGTTTCATTCCTTAAATTGTCAGATTCTTCCTTTGATCGAACTACAACTCACAAGTATTACAACAGAAATGATACATT
GAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATGCTCATCAGAATTATCTGCTATATCTGTCTCGC
AGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAGCAAGTTCTGAAGGTTCTTTGGAAATAACATCA
TTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCTGGCCGAAAGCATTTTAATAGCAATCCTTCAAA
TAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAACATATTTTCCAGGGAAGCTGTGCAATTCTGCT
GGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGAATGGTGATGGTCATTTTCCAGCCATTGTAGCA
TATATACTGTACATGGAGGGCCAATTACATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCAGCAGCTTGGACTAAGCCTGTTGACGATTCCTC
CACTGTGGTCTCTGGCAGTCGAAATGGTACAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTCAGACCAAGCACTTTACTGGAAGGTCTTACCTT
GCAGTATAGCTCGGCGAGCTGGACGCCAAGGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGCATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAG
>TRINITY_DN126397_c1_g2_i8 from sample A1 / Example unspliced isoform:AGAAATTGGGCATATGGCAGACTGGATCTACAAGGTTTAGTAATATGATTATTGTCAATGACTTCCACAGTTTGAACTGGTACAAAGTCCTTGCTTTTAGCAAGCTGTCTGGTTCTGTTGATATCAATTGATTTAGATCTGCTAACTGCCTCTTAGCTGAGGAATAGATATTCACATTCTTTGTAATAAATGCTATTATG
AGAAACTTAGATAATCGATCACATGTTGGGTGAGCTATTATTTGTGAGGATTATCACAATGTGGGAGTTTTAGCCCTCCACATGAAGTTCTGTGATTAAGAAGTTGCTTATGGGTTTAATGAATAAACTAGGAGTTCCAAATGGAAATAATTCAACATGGAGGTCATCTTTCTATAGGAGGAAGCACTAGAAACAAAAAA
ATTGCAATTGCCTCTGTCCAGCGACTGCTATTCTCATGGACTGACTGCTTTTCTGCATGTGTTTGTGGCCCTTAATTACTATAATGAAATAAGCTATGCCCTTATTAAGCCCACCCATTTGTCTGTCACTCTGCATTATAGGAAGTCTTACTGGTTTGCTCCATATGCACCTTTACCCTTGCAGTGTTTTCAACCATGGT
TGTCTGATTAGGCTACTGTCCTTATGACTCTGCTAAATCCCCACTATAACCATGAATAGGAAATAGACATCCAGGACTTTTGTCCCTATTCTTATCTCTAAATTGTTTGATCCTTTGAGGTATTTTTTATTGCTTTGTTTCAATATTCATATTTCCAAGCTTGTTGAATTTATAATGTTTAATTAATCTGCATTTTCACT
GTGTAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGATGAGATCATGGAAG
CCGAAGAAATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCATTTCAAGTCAAATC
CCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGATGTGTTGGTGTTTC
AAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTgtaagttaaacttctgatggcttgaattactggtcttctgt
tactttttctgtatgcatatttaactgaggttctttatgattataatgccgttatattattagactgcttttaccgaagaaactaattgtataaaaactaaaaagacatcattcatgcatggtccattgatcaatttcacgctttttttgatatcgtgtttgggaatctctgcttattcagatttgtttcaaggtccttg
tcatttgatggaatctttgttatttcttgcattaaaaagcttttgaaatttcaaaactatttattttttccctcccaattacattgtttgcaaatgccaattatatatctttccctttatcagGACTTTCGAGACATTTCAATATTCCACATTCGTAGCAATCTTCTGGTTCTGAATGGAAGCTTTCAATGGAGCCAATA
ATTTATAGCTTGCCAAGCTGGATAGTTTCATTCCTTAAATTGTATGCACTTTCTTTTGGTTTGATAGAGCTTTATATTTTCGTTTTAGTATAGTTCTAAGGATGGATGTTTTACTTCAAGATGCCTTTTGGTCACATCATGTTTGGAGCGAGTCGTAGGCTTGTCACATTTTAGGAGAAGAATCTTTATACCATTATTTT
CCATTGTTAATGATGCATAAGAAAGTAGATTCAGAGTTACAATGATTTTGAGACCAATTTGTATTGGATTTTATTTAATTCAAAGCATGTCTTTCAACTATACTGTCACAGGTCAGATTCTTCCTTTGATCGAACTACAACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAG
CTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATGCTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAG
GGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAGCAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGG
TTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCTGGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCAT
ATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAACATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAA
GTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGAATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTACA
TGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCAGCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTA
CAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTCAGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCA
Intron 7 GTCTGCAGTC ... TGCCTTACAG
| Sample | Retention score (%) | Exon junction reads | 5'ss reads | 3'ss reads |
| A1 | 25.8 | 105 | 39 | 34 |
| A2 | 19.4 | 81 | 28 | 11 |
| A3 | 19.55 | 72 | 14 | 21 |
| A4 | 18.97 | 79 | 29 | 8 |
| A5 | 16.44 | 61 | 9 | 15 |
| A6 | 0 | 2 | 0 | 0 |
| A7 | 22.22 | 35 | 4 | 16 |
| A8 | 23.81 | 8 | 4 | 1 |
| A9 | 17.81 | 30 | 7 | 6 |
| A10 | 23.29 | 28 | 6 | 11 |
>TRINITY_DN126397_c1_g2_i4 from sample A1 / Example spliced isoformTCTTGCTTGATTGATTGTTGAGGCTGGAAAATAAAAATGGAAGGCGATGAATACCTCTACTCAGTTTGCGCTAGTGCCATTTTGACGATCATCCGACTAGGTCGCGAGCGATGGGAGCCAACAGGTTCGAATCCGGGGAGGACATCGTGGGTAAGTCCGTGCAGAAGAAATTCAAGGGTTATGGGGTTTTCACGGGCAAA
GTCGTTTCCTACAACTCGGCCCGGGGGTTTTACAGGGTCAGGTATGAGGACGGGGACGCAGAGGAGCTAGACATGGACGATGTCCGTTGCATTCTGGTGCCAGAAAATGAAAATGGCAGGAAGAGGAAGCGCGACGATCAGACTCCGAAGCGGCGAAAGAGGAGGAGAAAGGATGGCGTCGGCCGGCCCAGAAAGACTAC
AAAGGAGGCTGAGGCTTCGAATAAGGGAGCTAGAGGGAGGAGTAGAAAAAAGGAAACTGAGGTTTCGAACACGGGACTTATTGGGGATAGTGGAGGAAAGGAGGTCGAGGTTTCGAACACGGGACTTATTGGGGATAGTAGAGGAAAGGAGGCAGAGGTTTCGAACACGGAACTAATTGGGGATAGTATAGGAAAGGAGG
CCGAGGTTTCGGACACGGGACCTCCTCTTGGAGGGAGTAGAGGAAAGGAGTTCGAGGATTCGAACACGGGACCTCATCTCGGAGGGAGCAGAGGAAAGGAGGTCAAGGATTCGAACACGGGAGATCTCGTAGGGAGCAGAGAAAAGGAGGCGGAGTTCTCGAACATGGAACCTCGTAAGAGGTTTAGAAGAAAGATGTTA
GAGATTTGGAAGACGGGACCTGTTAGGAGGAGTCCTCGTGTGAGGGAATCAGCGGAGAATGCTATCGAAAAGCCCACTCCGCCTAAGCAGCGCAGGTCCAGGGTTAGGGTTCGACATGACGATTTGGCACGGTCAATGCCCTCGGTGCCCCAACTGCCCCTTTCTTCCATCGATTTATGTGTGCCGGAGGAATCGGTGCC
GAATTTATTATATGTTTACAATTCTTTGAGGTCTTTTAGTAAATTGTTGTTTTTGAGTCCGTTCGGGCTTGATGATTTCGTTGGGGCATTGAACTGCAAGTCTGCTAATTCTTTACTAGATGCCGTGCACGTCAAGCTTCTGCAAGCGTTGAGGAGGCATCTTTATTCTCTTTCCAAAGAAGAGTGTTCTGCTGCTGCAG
ATGCGGCTAGATGTTTGAGGCACCTGGACTGGAATTTTCTAGATATGATAACCTGGCCAGTTTATTTGGTAGAATACCTAACAATGCAAAGGTTTAGTAAAACCTATGGCCAAAAGGTTGGTCATCTGAGGCTTTTGGATGGGGAGTACTATAGGATACCGGTGTCTATAAAACTTACTATTTTACAGTTCCTCTGTGAT
GAGATCATGGAAGCCGAAGAAATAAGGGCAGAAATTAGCATGCGGCTAAATGATGAGCCAGATGCAGAGACTGAGATAAAGGTTTCCCCTGGTGTGCGGTCAGTTGAAAATAAATTGAGGTCTGGGAGGGTACGCAGACGTCTATGGAAAGATGGTGAGGAATTGGACTATACTAAAATAGATGATCATGGAAGCTCCAT
TTCAAGTCAAATCCCTGTTGAGAATGTAAATTATGTGGGAGAAAAGACAACAGTTCAGCGCAAATTACGTGGCAGACCGCCCACTAAAGTTGAAAAAATAAGACCTGTAGAAAATTTGAACAGTGATGAGTGCTGCCTTTGTGGGATGGATGGTACTTTAATTTGTTGCGACAGTTGTCCGTCTGCATATCATTCCAGAT
GTGTTGGTGTTTCAAAAGCCCATTTACCTGAAGGGGATTGGTATTGTCCTGAGTGTGTTGTGAAAGAAAGCAGCAAAGGACTTGAGGCATTGAGTGGCTTACAAGGAGCAGAGATATTGGGTTTTGATCCACATGGACGTGTATACTTCTCTGCCTGCGGTTACTTGTTAGTGTCAGATTCTTCCTTTGATCGAACTACA
ACTCACAAGTATTACAACAGAAATGATACATTGAAGGTTTTGAAGATATTAGACTCATCAGCTCCTCTATATGATAATATACAGAATGCTATAGTACAATGCCGGGATATGCCAATCAGTAATGGTGGTGCGCCAAAGCATAAAATGGGCACAATGGATCCATCATGGCTTACAAAAGCAGATAATTCTTCTGATAAATG
CTCATCAGAATTATCTGCTATATCTGTCTCGCAGTCTTTTGTTCAACAGGATCAGAAGAAGGGATGTCCCCAAAAGGATGTAAGCCTAAAAAAAGTGCCATCTCGAAAATTGGAGGCTCTCCAGCATCATGATGCAGCGGGACAGAAAGAAGATTATGAAATGCTTGTCATGGATCAGAGTAATGATAATACTCAGTTAG
CAAGTTCTGAAGGTTCTTTGGAAATAACATCATTGAATGATGTAAAAGGACATCAGAAAGGTTCGGGTGCTCATTGTAGTCTTGGATTTACTAAAAGCAGTGCATTACAAGAAGAACTAATTACTGAAACGGATGCTGGCAGTGCAGCTGTCCAAGTGAAACAAGTAAAGCAGTCTGATATGGGCACTGGCTTTAAAGCT
GGCCGAAAGCATTTTAATAGCAATCCTTCAAATAGGGAAGTGACCTTCCATCCAAATGCATATATCAATCAGTATGTTTTAGGTGATATGGCTGCATCAGCAGCTGCAAATTTAGCAGTCAAAGGTGCTCAACTTCGACGAGTCAATGTACGAGAATCTTTGAAGAAATCTTCATCAGCAGCACTTGCAGAACAAGTAAA
CATATTTTCCAGGGAAGCTGTGCAATTCTGCTGGCCATGTTTTACAAAGAAAGTTATGGAAGTGCCAAAGGAAGGATGTGGGTGGTGCTTTGTGTGCAAGACATCTCCAGGTAGCCGCAGCCGTTGTTTGTTGAATATAGCTGCTGGAAATATTATGACAGGAGCTGCACGGATGGCAGGTGGCCTCCGTCCAACAAAGA
ATGGTGATGGTCATTTTCCAGCCATTGTAGCATATATACTGTACATGGAGGGCCAATTACATGGCCTTTTGGAGGGTCCATGGGAGAATATATCCTACAGAAAATATTGGCGCAAGTCTATTGAACAAGCTTCAACTGTTGCAGAAGTCAAATATTATCTTTTGGAGCTAGAGACGAATATCAGACTTGTTGCACTTTCA
GCAGCTTGGACTAAGCCTGTTGACGATTCCTCCACTGTGGTCTCTGGCAGTCGAAATGGTACAACTGTAGCTGGCCTAACACCAAAACGTGGGGGTCGTTTTCAACGTGGTCAAAGGCATCCTAGTGCTGCAAAAATAATCTCTGCTTCAATGGATGCTAAAATGTTTGGAGTTAATTGGTGGAGGGGCGGAAAATTTTC
AGACCAAGCACTTTACTGGAAGGTCTTACCTTGCAGTATAGCTCGGCGAGCTGGACGCCAAGGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGCATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAGATAAGGAATCTTG
ATGCTTGCATTAGATGGGATGATCTTCTAAATTCAGAAGATTTCTGTCAACCTAAAATGGTAGGAGACAAAGAGTCTGCAGTCTTCAAGAAGGCTGTTATTTGTGCCAAGAATTCAGAAAATGGTCTGATAAAGTACTTGATAGACTTTGGGAAACAGAAAGCCATTCCAACCTGCATTGTTAAATATGGAACAAAGCTT
GAAGAGGCTTTAGATGGTACATGCAAGTATTGGCTAAGCGAGTCTCATGTGCCTCTGTGCTTGCTGAAGGATTATGAAGAGGAAGTGCGATTACATGCCCACCCAAACAAAGGTTCAAAGAGATTCCGTAAGGTTCATTATAGACTCTCAAAAACTTCCAAGTTGGACGTATTTACCATCCTCTTGGCAAAGGCAGAGAA
TCGTGAGAAATGTTCATGTACCCATTGCAACAAGGATGTACCAATCCGGAAAGCAGTTCGATGCCATTTTTGTCAAGGTTCATTTCATCGGAAGTGTTCGATATCTTCCCATTTCTTGAATAAACTGCAGTACACATTTACCTGTTACAAGTGCCACAGTGTTAAGCTGCTAAAGAGCAAGCCTGAAATTAGAGTGCAAT
CAGGGAAGAAGATTTTGGTGCAATCAGGGAAGAAGATTTTGTCAACATGCAAAAGGCGCAGGCTTTCAAAAAAAGTAGAGGAAATTGGGATGTCTACACGAGATGTAAAGCTAAAGAAACCTAAAAGGCATAGGTTGCATAATGATAATGTTGCAATAAAAAAGCATGACAGTGTTGGGAAGGTCACAGTTCGGAGAAAG
AAAATCAAAGGAGATGTTCTTTCGGATGGTTTTTTATGGAAGAAAAATGCGACCGATGAATTAAGAAAGGATTCTAGGCAACGCATGCTTCTCCTTCCAGGCCAGAATGGCATGGATGCCTCTCTATGTCCTAAATGTATTCTTTGTGGCGAGGCATACGACCCTCAATTTTTATATGTTGGATGTGAATATTGTAAAGA
TTGGTTTCATGGAGATGCTTTTGGGCTTACCGTGGAGAATATCATTGATGTGGTTGCTTTCAAGTGTTACAAATGCTGCAAGAATATTGCACCCATCTGTCCTTGTGCCAAGAAGGATAATCTGTGTGATAAAAGGCTTGGTTTGAAGATTACAACGGATAATGAAGTGCTGGATGTAGCCAAAAATGGCAAGCCCTTAG
ATGTGATATACAGCAGATCTAAACTTGTTTCTGATGAGGAGCTTCATGCTAAGTTAGCCAGGGAAGAAGAGCATATATTGACTCAAGTAGAATATGATTCAGAGAATCGTCCTCAAGATCCTAAAGTAGGGAACAATAGGCCAGTTCCAGAAGCAGAAGAGATTCCAAATTCAGCATGCGTTATAGTCAGTGAACAGTTG
CTAACCCCCAAAATAGACCGTGACCCATCTGTTAACAATTATCTGCAGGAAGAAATGGTGAATTGCCAGCAATATGAGCAAGATGACAGGTCAAGGGAGGAAACTGTTGGATCTGAAGTACATAAAACAGAGCCCATCCAGATTTATCCTGATACACAACCCCAATCCATGCTTTCCTGTACTGAATTATTGGCTTCTGA
GGATGATCAAGTAGATCTTATTGATCCATCACCAAATGTTGCTGGAAATTATTCTGAAAATTTACAGCCTGTTGACAAGGGAGAATATGCATTTTCTCAACAATTAAATGTTCAAAATTGTTCCGAAAATTTACTGCCTGTCGAGGAGGTAAATGCATTTTCTCAACTACCAAATGTTCAAAATTGTTCTGAAAATTTAC
TGCCTTTTGAGGGAGAAGATACATTTTCTCAACTACCAAATGTTCAAAATTGTTCTGAAACTTTTCTGCTTGTTGAGGAGGGAGAAGTTGCATTTTCTCAACTACCAATTGTTCAAAATTGTTCTGAAACTTTAGTGCCTGCTGACGAGGAAGAAAAGACATTATCTCAACTACAGGGCATAAATGATGGTGTTCATCAT
TCCTCAGGTGATGTTATTTCACCAGACCTTTCAGAAAACAAAGGGTGCCATCTAGCAAATAGATTTTCATTGGAAAATTTCATAAATCAACCATATAGAATAGGTGGTGAGATTACTTCAGAAGAGATGGAAAAGAAGATATGTTCAACGTGCAACTCAATAGAGGTGCCTCCTGATCTTCTGTGCAGTGGATGTGGCCT
TGCCATTCATTGCCATTGTTATGAACAAGCACCCACTGGCCTTTGGACTGCATCTGGATGGATTTGTGGTTGTTGTCAGGATGGTTTTGTACCAGGCTAAGGCATCCTTTGGGGAATGCCAGAAGGCTCTGGTTAGATCAATACTTTTTGCCCAACAAAGGCCTGATCCCATATTATGATGATCAAGTCGACGAGGACTT
TCATATATGAACGGGAATCGATCTTCTCTATTATTCTCCATGAACCTTTCCAGTAGCTTCCTTCTGCAAGGGTGAAACAATGGGAGCAATGGTGTTCCGACTTGTTCATCCCTGATTAGAAGACTACAATTGTGGAACTATTTTCTTGGATACCATTTGTAAATTGTTATTGTTTTGCAGCATCCACTAGCTTTTCCTGT
CAAGGTGGAATTCACTGAGATAGAACAAACACTCGAATAGAACGAAAAAAATGAAAGTGCCTGGTGTTTGTGTATATGTTTTTGTAACACCCAGATTTCTCTGGCATTACAAAAGTGGACGACTGTCTCATTAAAACTGTAAGTGTATCCCATATTCATAAGCTCATTCTTTTTCCATCCATTGTTCCTGATGATCTCTG
CTTTTAAGAGTCAAGCAGTTTGTGTCTTGAACAATCTAAAAAGAGCTATTTATTGTGAAGCTTCCATACATATCTTTAAATAGTGATGACGTAACAGAGCTTTGGCTTCTCTCTGTTAACCATTTTCTTACATTCTCTATACATATCATTAAATAGTGATGACGTAACAGAGCTTTGGCTTCTCTCTGTTAACCATTTTC
TTACATTCTCTATACATATCATTAAATAGTGATGACGTAACAGA
>TRINITY_DN126397_c1_g2_i6 from sample A1 / Example unspliced isoform:CTCGGCGAGCTGGACGCCAAGGTTTTTTTTTCATTCTTTTCTGAGTTCCTTATTGTCACTATATCTTTGTTCATTTTGGTTTAGTTTGACTGAGGTTTTTAAATGCACACATATTATAAATTTGCAGGTGGCTTTAGAAGGATACCCGGTATCATATACAATGAAGGTTCAGAAGTCCCCAGAAGAAGTAGACAGTATGC
ATGGCGAGCTGCTGTTGAAATGTCAAGGAGTGTATCACAACTAGCTGTACAGgtctgcagtcagtttgcaattccattatttttctgaggtttgaagcaattggccttatattattcttatattcttaaaatgccttacagATAAGGAATCTTGATGCTTGCATTAGATGGGATGATCTTCTAAATTCAGAAGATTTCTG
TCAACCTAAAATGGTAGGAGACAAAGAGTCTGCAGTCTTCAAGAAGGCTGTTATTTGTGCCAAGAATTCAGAAAATGGTCTGATAAAGTACTTGATAGACTTTGGGAAACAGAAAGCCATTCCAACCTGCATTGTTAAATATGGAACAAAGCTTGAAGAGGCTTTAGATGGTACATGCAAGTATTGGCTAAGCGAGTCTC
ATGTGCCTCTGTGCTTGCTGAAGGATTATGAAGAGGAAGTGCGATTACATGCCCACCCAAACAAAGGTTCAAAGAGATTCCGTAAGGTTCATTATAGACTCTCAAAAACTTCCAAGTTGGACGTATTTACCATCCTCTTGGCAAAGGCAGAGAATCGTGAGAAATGTTCATGTACCCATTGCAACAAGGATGTACCAATC
CGG