NA ----> NA

Intron 1 GTTGGTCGTT ... GCACCGAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	1	1
A2	66.67	1	2	0
A3	50	2	2	2
A4	-1	0	0	0
A5	0	2	0	0
A6	29.17	17	7	0
A7	14.29	18	3	0
A8	24	19	6	1
A9	13.16	33	5	0
A10	20	16	4	0

>TRINITY_DN41388_c0_g1_i13 from sample A8 / Example spliced isoform

GCGTACAAAGGTTGTGGTAGAGTTGGTGTTTGAGGAAGGTTTTATGGAGATTGACGAGAGCGTGACTCTCCCATCGTTAGGAAAAGAGGACAATTCTCGTGCTGTAAGCGTCAGCTCCAGAGAAAGTTCTGTTTCGTCTTCTTCTCGTCGTTCTTCCAAGTCTGGGGTTTCTACCAGTACTGGGGTTTCTGTCACTACAG
CTCTCCCCACGCTTGGTCAGGATCAAAATGCCTGCAGACCCCTTACCAGAGAGGAGGAGGAAAAAAGAATCACTGATTTTGTTGAGAATGCAGCTGAAGAATACGAACAGTAAGTTTTTATATTTATTCATGCTTGCAACTCTAACTGATTTCAAGTTGTTTTTTTTCCATGTAACTAAGCACCCCACCCTCTAGTCCAT
ATGCAAGTGTAGGATATTCTTCATGGTTCATAACCTCAATAACAATCGACTCACAAAAAGCAAAACATATCACAAGCAGGATTTGAACCCGTGGAGAATAACATTTCTCCTCCCTGACCATTCAAGCTAAAACCCAATTGACCATTGATTTCAAGTTGTGTTTGTTAATACAACATTAATATTAATCATCATATTGTTTC
ACAACTACAGACAAGTGGCTTACCACATAAATCAACAGCAAGATTGCAGAATATGCAAATGTTATAGTCTCCCTCGAGATGATCCAGGAAACATCGTATTAAAGATTGTAGAATAGGACTTCACCCTGGCTATGTTTGCAAACTTTGCCTAAAAAGAGAAGATCATGTGATTCAGAATTGTCACAAGCCAATAAATCAAG
CAGTCAAGTGTAATAAATGCAACAGAGGAACACATGTTGTCTATGCCTGTCCCTTCACTTGATAAATAAACAAGGGAGCGAAGGTATGGATGTAATTAGTTGCATTTACATGTCATTACTGGTTTACACCCGAGTTCACATGGAGGAGCCTGCTGGATTTCACATCTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGA
TTATTGCATAATATCTATTTTCTTGTGTCATATGACTAAGTAGAGTATGTTTGTTCTTTATCCCAAGTAGAGTAGAGGACAAACCAGGTAGAGTATGGGATCAACAGTTTAGTCTATTTTACATGTATTGATCTGAGCAAATCAGGAGACTCATTTCAAAATTAATCACATGCCCATTTTCAAAACATGTTTTTAAGCTT
TGTTTACTTTGATTATTGCGTAATATATATTTTAATATCTATTTTCTTGTG

>TRINITY_DN41388_c0_g1_i12 from sample A8 / Example unspliced isoform:

GCGTACAAAGGTTGTGGTAGAGTTGGTGTTTGAGGAAGGTTTTATGGAGATTGACGAGAGCGTGACTCTCCCATCGTTAGGAAAAGAGGACAATTCTCGTGCTGTAAGCGTCAGCTCCAGAGAAAGTTCTGTTTCGTCTTCTTCTCGTCGTTCTTCCAAGTCTGGGGTTTCTACCAGTACTGGGGTTTCTGTCACTACAG
CTCTCCCCACGCTTGGTCAGGATCAAAATGCCTGCAGACCCCTTACCAGAGAGGAGGAGGAAAAAAGAATCACTGATTTTGTTGAGAATGCAGCTGAAGAATACGAACAGTAAGTTTTTATATTTATTCATGCTTGCAACTCTAACTGATTTCAAGTTGTTTTTTTTCCATGTAACTAAGCACCCCACCCTCTAGTCCAT
ATGCAAGTGTAGGATATTCTTCATGGTTCATAACCTCAATAACAATCGACTCACAAAAAGCAAAACATATCACAAGCAGGATTTGAACCCGTGGAGAATAACATTTCTCCTCCCTGACCATTCAAGCTAAAACCCAATTGACCATTGATTTCAAGTTGTGTTTGTTAATACAACATTAATATTAATCATCATATTGTTTC
ACAACTACAGACAAGTGGCTTACCACATAAATCAACAGCAAGATTGCAGAATATGCAAATGTTATAGTCTCCCTCGAGATGATCCAGgttggtcgttggatattatgatctcactctttttgtgctccctcatctcatttgttttgtgcttccttaactgttttatgttggttgtacttttatgatcccatgtggtcatt
tgtgcgcacacacacacacatataccagcatagatccatctttaagcaccgagagGAAACATCGTATTAAAGATTGTAGAATAGGACTTCACCCTGGCTATGTTTGCAAACTTTGCCTAAAAAGAGA

Intron 2 GTAAGTTTTT ... CTAAAAAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	2	3	0
A2	0	2	2	0
A3	100	0	0	1
A4	50	2	0	2
A5	100	0	0	2
A6	62.5	15	3	25
A7	62.26	20	12	33
A8	69.05	13	6	29
A9	57.58	28	12	38
A10	60	16	6	24

>TRINITY_DN41388_c0_g1_i1 from sample A8 / Example spliced isoform

GCGTACAAAGGTTGTGGTAGAGTTGGTGTTTGAGGAAGGTTTTATGGAGATTGACGAGAGCGTGACTCTCCCATCGTTAGGAAAAGAGGACAATTCTCGTGCTGTAAGCGTCAGCTCCAGAGAAAGTTCTGTTTCGTCTTCTTCTCGTCGTTCTTCCAAGTCTGGGGTTTCTACCAGTACTGGGGTTTCTGTCACTACAG
CTCTCCCCACGCTTGGTCAGGATCAAAATGCCTGCAGACCCCTTACCAGAGAGGAGGAGGAAAAAAGAATCACTGATTTTGTTGAGAATGCAGCTGAAGAATACGAACAAAGATCATGTGATTCAGAATTGTCACAAGCCAATAAATCAAGCAGTCAAGTGTAATAAATGCAACAGAGGAACACATGTTGTCTATGCCTG
TCCCTTCACTTGATAAATAAACAAGGGAGCGAAGGTATGGATGTAATTAGTTGCATTTACATGTCATTACTGGTTTACACCCGAGTTCACATGGAGGAGCCTGCTGGATTTCACATCTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGATTATTGCATAATATCTATTTTCTTGTGTCATATGACTAAGTAGAGTATG
TTTGTTCTTTATCCCAAGTAGAGTAGAGGACAAACCAGGTAGAGTATGGGATCAACAGTTTAGTCTATTTTACATGTATTGATCTGAGCAAATCAGGAGACTCATTTCAAAATTAATCACATGCCCATTTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGATTATTGCGTAATATATATTTTAATATCTATTTTCTTG
TG

>TRINITY_DN41388_c0_g1_i13 from sample A8 / Example unspliced isoform:

GCGTACAAAGGTTGTGGTAGAGTTGGTGTTTGAGGAAGGTTTTATGGAGATTGACGAGAGCGTGACTCTCCCATCGTTAGGAAAAGAGGACAATTCTCGTGCTGTAAGCGTCAGCTCCAGAGAAAGTTCTGTTTCGTCTTCTTCTCGTCGTTCTTCCAAGTCTGGGGTTTCTACCAGTACTGGGGTTTCTGTCACTACAG
CTCTCCCCACGCTTGGTCAGGATCAAAATGCCTGCAGACCCCTTACCAGAGAGGAGGAGGAAAAAAGAATCACTGATTTTGTTGAGAATGCAGCTGAAGAATACGAACAgtaagtttttatatttattcatgcttgcaactctaactgatttcaagttgttttttttccatgtaactaagcaccccaccctctagtccat
atgcaagtgtaggatattcttcatggttcataacctcaataacaatcgactcacaaaaagcaaaacatatcacaagcaggatttgaacccgtggagaataacatttctcctccctgaccattcaagctaaaacccaattgaccattgatttcaagttgtgtttgttaatacaacattaatattaatcatcatattgtttc
acaactacagacaagtggcttaccacataaatcaacagcaagattgcagaatatgcaaatgttatagtctccctcgagatgatccaggaaacatcgtattaaagattgtagaataggacttcaccctggctatgtttgcaaactttgcctaaaaagagAAGATCATGTGATTCAGAATTGTCACAAGCCAATAAATCAAG
CAGTCAAGTGTAATAAATGCAACAGAGGAACACATGTTGTCTATGCCTGTCCCTTCACTTGATAAATAAACAAGGGAGCGAAGGTATGGATGTAATTAGTTGCATTTACATGTCATTACTGGTTTACACCCGAGTTCACATGGAGGAGCCTGCTGGATTTCACATCTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGA
TTATTGCATAATATCTATTTTCTTGTGTCATATGACTAAGTAGAGTATGTTTGTTCTTTATCCCAAGTAGAGTAGAGGACAAACCAGGTAGAGTATGGGATCAACAGTTTAGTCTATTTTACATGTATTGATCTGAGCAAATCAGGAGACTCATTTCAAAATTAATCACATGCCCATTTTCAAAACATGTTTTTAAGCTT
TGTTTACTTTGATTATTGCGTAATATATATTTTAATATCTATTTTCTTGTG

Intron 3 GTACCCATTT ... GCAACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.86	4	0	3
A2	77.04	1.2	3.5	4
A3	28.7	2.6	0.3	1
A4	32.41	2.3	1	1
A5	0	2.6	0.5	0
A6	28.77	40.6	8.7	16
A7	30.94	40.3	5.6	18
A8	30.3	39.9	9.3	17
A9	31.97	40.7	14.7	19
A10	24.93	22.2	6.3	7

>TRINITY_DN40618_c0_g1_i7 from sample A6 / Example spliced isoform

GGGAGTTTTTATCTCTGATTGACTTAAACTGCATCGGGTAGTTCTGATTAGTGAGAAACTGCATCGGTAGTTATAATTAGTTAAGCCTCCAGGGCCATTTTCTATCAGGCTCTGCAAATTTCCACACGGCGGGATGTTTAATAATAATACAGTTCTTGTAATTTGCCTCTGCATGCGTGTGAAGAAGAGGGATAAATTCA
ATTCAATCCATAAAAATAGAGATCATGGCAGAGCGGCAGTACATTTCATATAAATTCAGAGACTGGCAAAACTTCCGACATATGGAAATTTCAGTAAAGGATGAAGAAGCCGGTATGCTGTTGACGGATCTCAAAGAAGCAATCATAGAAGACCGTAATTTTAACAAGCCCAAATTCAAGGTCACCGATTATGAAACCAG
CCGAGAGTATGAGGACAATGACAAGATAGCGGCGCGTACAAAGGTTGTGGTAGAGTTGGTGTTTGAGGAAGGTTTTATGGAGATTGACGAGAGCGTGACTCTCCCATCGTTAGGAAAAGAGGACAATTCTCGTGCTGTAAGCGTCAGCTCCAGAGAAAGTTCTGTTTCGTCTTCTTCTCGTCGTTCTTCCAAGTCTGGGG
TTTCTACCAGTACTGGGGTTTCTGTCACTACAGCTCTCCCCACGCTTGGTCAGGATCAAAATGCCTGCAGACCCCTTACCAGAGAGGAGGAGGAAAAAAGAATCACTGATTTTGTTGAGAATGCAGCTGAAGAATACGAACATTATTCTACATTGCAGGGAAACATCGTATTAAAGATTGTAGAATAGGACTTCACCCTG
GCTATGTTTGCAAACTTTGCCTAAAAAGAGAAGATCATGTGATTCAGAATTGTCACAAGCCAATAAATCAAGCAGTCAAGTGTAATAAATGCAACAGAGGAACACATGTTGTCTATGCCTGTCCCTTCACTTGATAAATAAACAAGGGAGCGAAGGTATGGATGTAATTAGTTGCATTTACATGTCATTACTGGTTTACA
CCCGAGTTCACATGGAGGAGCCTGCTGGATTTCACATCTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGATTATTGCATAATATCTATTTTCTTGTGTCATATGACTAAGTAGAGTATGTTTGTTCTTTATCCCAAGTAGAGTAGAGGACAAACCAGGTAGAGTATGGGATCAACAGTTTAGTCTATTTTACATGTAT
TGATCTGAGCAAATCAGGAGACTCATTTCAAAATTAATCACATGCCCATTTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGATTATTGCATAATATCTATTTTCTTGTGTCATATGACTAAGTAGAGTATGTTTGTTCTTTATCCCAAGTAGAGTAGAGGACAAACC

>TRINITY_DN40618_c0_g1_i9 from sample A6 / Example unspliced isoform:

GGGAGTTTTTATCTCTGATTGACTTAAACTGCATCGGGTAGTTCTGATTAGTGAGAAACTGCATCGGTAGTTATAATTAGTTAAGCCTCCAGGGCCATTTTCTATCAGGCTCTGCAAATTTCCACACGGCGGGATGTTTAATAATAATACAGTTCTTGTAATTTGCCTCTGCATGCGTGTGAAGAAGAGGGATAAATTCA
ATTCAATCCATAAAAATAGAGATCATGGCAGAGCGGCAGTACATTTCATATAAATTCAGAGACTGGCAAAACTTCCGACATATGGAAATTTCAGTAAAGGATGAAGAAGCCGGTATGCTGTTGACGGATCTCAAAGAAGCAATCATAGAAGACCGTAATTTTAACAAGCCCAAATTCAAGGTCACCGATTATGAAACCAG
CCGAGAGTATGAGGACAATGACAAGATAGCGGCGCGTACAAAGGTTGTGGTAGAGTTGGTGTTTGAGGAAGGTTTTATGGAGATTGACGAGAGCGTGACTCTCCCATCGTTAGGAAAAGAGGACAATTCTCGTGCTGTAAGCGTCAGCTCCAGAGAAAGTTCTGTTTCGTCTTCTTCTCGTCGTTCTTCCAAGTCTGGGG
TTTCTACCAGTACTGGGGTTTCTGTCACTACAGCTCTCCCCACGCTTGGTCAGGATCAAAATGCCTGCAGACCCCTTACCAGAGAGGAGGAGGAAAAAAGAATCACTGATTTTGTTGAGAATGCAGCTGAAGAATACGAACAGGAAACATCGTATTAAAGATTGTAGAATAGGACTTCACCCTGGCTATGTTTGCAAACT
TTGCCTAAAAAGAGAAGATCATGTGATTCAGAATTGTCACAAGCCAATAAATCAAGCAGTCAAGTGTAATAAATGCAACAGAGgtacccatttcacattccaattgaatttcatcaataatattatatgttttaaataaatgttttaatttcatattcatataatgcaactacagGAACACATGTTGTCTATGCCTGTCC
CTTCACTTGATAAATAAACAAGGGAGCGAAGGTATGGATGTAATTAGTTGCATTTACATGTCATTACTGGTTTACACCCGAGTTCACATGGAGGAGCCTGCTGGATTTCACATCTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGATTATTGCATAATATCTATTTTCTTGTGTCATATGACTAAGTAGAGTATGTTT
GTTCTTTATCCCAAGTAGAGTAGAGGACAAACCAGGTAGAGTATGGGATCAACAGTTTAGTCTATTTTACATGTATTGATCTGAGCAAATCAGGAGACTCATTTCAAAATTAATCACATGCCCATTTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGATTATTGCATAATATCTATTTTCTTGTGTCATATGACTAAG
TAGAGTATGTTTGTTCTTTATCCCAAGTAGAGTAGAGGACAAACC

Intron 4 GTAAGTTTTA ... ACAACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.27	4	3	0
A2	14.34	6.4	2	0.1
A3	42.86	2	0	3
A4	-1	0	0	0
A5	4.76	1.4	0	0.1
A6	33.08	10.9	3.6	7.1
A7	30.34	16.7	12	2.4
A8	31.38	17	6	9.6
A9	33.48	20.6	12	8.4
A10	50.69	5.7	6	5.4

>TRINITY_DN41388_c0_g1_i6 from sample A8 / Example spliced isoform

GCGTACAAAGGTTGTGGTAGAGTTGGTGTTTGAGGAAGGTTTTATGGAGATTGACGAGAGCGTGACTCTCCCATCGTTAGGAAAAGAGGACAATTCTCGTGCTGTAAGCGTCAGCTCCAGAGAAAGTTCTGTTTCGTCTTCTTCTCGTCGTTCTTCCAAGTCTGGGGTTTCTACCAGTACTGGGGTTTCTGTCACTACAG
CTCTCCCCACGCTTGGTCAGGATCAAAATGCCTGCAGACCCCTTACCAGAGAGGAGGAGGAAAAAAGAATCACTGATTTTGTTGAGAATGCAGCTGAAGAATACGAACAACAAGTGGCTTACCACATAAATCAACAGCAAGATTGCAGAATATGCAAATGTTATAGTCTCCCTCGAGATGATCCAGGTTGGTCGTTGGAT
ATTATGATCTCACTCTTTTTGTGCTCCCTCATCTCATTTGTTTTGTGCTTCCTTAACTGTTTTATGTTGGTTGTACTTTTATGATCCCATGTGGTCATTTGTGCGCACACACACACACATATACCAGCATAGATCCATCTTTAAGCACCGAGAG

>TRINITY_DN41388_c0_g1_i13 from sample A8 / Example unspliced isoform:

GCGTACAAAGGTTGTGGTAGAGTTGGTGTTTGAGGAAGGTTTTATGGAGATTGACGAGAGCGTGACTCTCCCATCGTTAGGAAAAGAGGACAATTCTCGTGCTGTAAGCGTCAGCTCCAGAGAAAGTTCTGTTTCGTCTTCTTCTCGTCGTTCTTCCAAGTCTGGGGTTTCTACCAGTACTGGGGTTTCTGTCACTACAG
CTCTCCCCACGCTTGGTCAGGATCAAAATGCCTGCAGACCCCTTACCAGAGAGGAGGAGGAAAAAAGAATCACTGATTTTGTTGAGAATGCAGCTGAAGAATACGAACAgtaagtttttatatttattcatgcttgcaactctaactgatttcaagttgttttttttccatgtaactaagcaccccaccctctagtccat
atgcaagtgtaggatattcttcatggttcataacctcaataacaatcgactcacaaaaagcaaaacatatcacaagcaggatttgaacccgtggagaataacatttctcctccctgaccattcaagctaaaacccaattgaccattgatttcaagttgtgtttgttaatacaacattaatattaatcatcatattgtttc
acaactacagACAAGTGGCTTACCACATAAATCAACAGCAAGATTGCAGAATATGCAAATGTTATAGTCTCCCTCGAGATGATCCAGGAAACATCGTATTAAAGATTGTAGAATAGGACTTCACCCTGGCTATGTTTGCAAACTTTGCCTAAAAAGAGAAGATCATGTGATTCAGAATTGTCACAAGCCAATAAATCAAG
CAGTCAAGTGTAATAAATGCAACAGAGGAACACATGTTGTCTATGCCTGTCCCTTCACTTGATAAATAAACAAGGGAGCGAAGGTATGGATGTAATTAGTTGCATTTACATGTCATTACTGGTTTACACCCGAGTTCACATGGAGGAGCCTGCTGGATTTCACATCTTCAAAACATGTTTTTAAGCTTTGTTTACTTTGA
TTATTGCATAATATCTATTTTCTTGTGTCATATGACTAAGTAGAGTATGTTTGTTCTTTATCCCAAGTAGAGTAGAGGACAAACCAGGTAGAGTATGGGATCAACAGTTTAGTCTATTTTACATGTATTGATCTGAGCAAATCAGGAGACTCATTTCAAAATTAATCACATGCCCATTTTCAAAACATGTTTTTAAGCTT
TGTTTACTTTGATTATTGCGTAATATATATTTTAATATCTATTTTCTTGTG