NA ----> NA

Intron 1 GCTATCTTGG ... AGTATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.48	19	154	89
A2	83	17	95	71
A3	84.8	19	137	75
A4	84.19	17	126	55
A5	81.67	11	70	28
A6	87.53	26	210	155
A7	83.41	38	231	151
A8	81.89	47	263	162
A9	81.85	45	267	139
A10	75.9	50	177	138

>TRINITY_DN44238_c2_g1_i9 from sample A9 / Example spliced isoform

CTCACTTACGCCAAGGTGCAAAGAACGAAGTGAAAATTATATTTCACAGTAAAATAAATTAAATTTAAGGCAAATGAGACCGTTGGAGAAGTTTCATTTCACTCTCTCGTTTTTCGCACTGCGGAGACATACAAAGGTTTTCACTGGAGAAATCATTCTCATCATGTTGTGGTAGGGCACTGTAGCGATTTCAGCACAGA
GAATGTCTTCAAAATCACGACGGCACGGCATCGTTGAAAATGGTGGGAAATTGTTTATCAAGAAAGGGTTTGTTCTCTGCCCCTCATTTCCACTTCTCAGCCGGAGAAACCCTGAACAAGCGGTACACCGAGTACTGTTTCCAAATCTTCGTAAGTTACATTCATGACCTCCTTGAAATCGAGGGCTGTTTCAGAAGTTT
CATTTCACACTCTCGTTTTTCGCAGTGCGGAGAGAAGAAGCGGTACACCGAGGGTTGTTTCCAAATCTGCGTATGCAATTCTAGAAATATTCCTCTAGCCAAAGTTCCACAAAAGTTCCATTTTCATGCGGAAGAGTTATTTCTGACTCATCGAGGAGAATATTATCAAGCTTTTCCACAGGTATTCTGATGCTAATTTT
TGGCCAGATTTGTCATTTCTGTAATGTTATCGGATGAACTAGCAGGACTAGTTTCCGATGACAAATATGAGAGA

>TRINITY_DN44238_c2_g1_i24 from sample A9 / Example unspliced isoform:

CTCACTTACGCCAAGGTGCAAAGAACGAAGTGAAAATTATATTTCACAGTAAAATAAATTAAATTTAAGGCAAATGAGACCGTTGGAGAAGTTTCATTTCACTCTCTCGTTTTTCGCACTGCGGAGACATACAAAGGTTTTCACTGGAGAAATCATTCTCATCATGTTGTGGTAGGGCACTGTAGCGATTTCAGCACAGA
GAATGTCTTCAAAATCACGACGGCACGGCATCGTTGAAAATGGTGGGAAATTGTTTATCAAGAAAGGGTTTGTTCTCTGCCCCTCATTTCCACTTCTCAGCCGGAGAAACCCTGAAGAAGCGGTACACCGAGGGTTGTTTCCAAATCTGCgctatcttggtcagggcagatcatctggaagttggtcagggcagatcatc
tggaagtacattcatatgctctttagtcaatggcttcagtatttcagGTATGCAATTCTAGAAATATTCCTCTAGCCAAAGTTCCACAAAAGTTCCATTTTCATGCGGAAGAGTTATTTCTGACTCATCGAGGAGAATATTATCAAGCTTTTCCACAGGTATTCTGATGCTAATTTTTGGCCAGATTTGTCATTTCTGTA
ATGTTATCGGATGAACTAGCAGGACTAGTTTCCGATGACAAATATGAGAGA

Intron 2 GCAATGGAGT ... TTCAACATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.95	86	155	92
A2	49.87	97	111	82
A3	55.61	95	144	94
A4	59.52	85	150	100
A5	60.25	32	55	42
A6	54.11	67	91	67
A7	51.31	121	160	95
A8	60.47	50	104	49
A9	62.58	90	202	99
A10	64	72	169	87

>TRINITY_DN44238_c2_g1_i3 from sample A9 / Example spliced isoform

CCGTTGGAGAAGTTTCATTTCACGGGAACAAGCGGTACACCGAGTACTGTTTCCAAATCTTCGTAAGTTACATTCATGACCTCCTTGAAATCGAGGGCTGTTTCAGAAGTTTCATTTCACACTCTCGTTTTTCGCAGTGCGGAGACATACAAAGGTTTTCACTGCACAAATCCGTCTCATTTGCGGTTGAGCAATGGCGC
GATTTCAGCACAGAGAAATCCGTTTTTGTCCATCTCATCTTGTGGTAGGGCAATGGAGCGATTTTGTCCATCTCATCTTGTGGTAGGCAATGGAGCGATTTCAACAGAGGAATCCGTTTTTGTCCTTCTCATCTTGTGGTAGGGGAATGGAGCGATTTCAGCACAGGGAAATCTGTATGATTTTCGTTGTGCGGAGACAT
ACAAAGTCTTCAAAATCACGACGACACGACATCATTGAAAATTGTCGGAAATTGTTTATCAAGAAAGGGGTTGTTCTCTGCCCCTCATTTTCACTTCTCAGCCGGAGAAACCCTGAAGAAGCAGTACTCCTGCGGTAGGGCAATGTATCGATTTCAGCACAGTGAATGTCTTCAAATCACGATGGCATGGCATCATTGGA
AAGTGTGGGAAATTGTTTATGAAGTGAGGGTTTGTGGTAGGGCAATGTAGCGATTTCAGCACGGAGAATGTTTTCAAAATCACGACGGTACGACATCATTGAAAATCGTCGAAATTGTTTATCAAGAAAGGGGTTGTTCTCTGCCCCTCAAAATTGTTTATCAAGAAAGGGTTTATTCTCTGCCCCTCGTTTCCAGTTCT
CAGCCGGAGAAGCCCTGAAGAAGCGGTACACCGAGGGTTGTTTCCAAATCTGCGCTATCTTGGTCAGGGCATATCCTCTGGAAGTACATTCATATGCTCTTTAGTCAATGGCTTCAGTATTTCAGGTATGCAATTCTAGAAATATTCCTCTAGCCAAAGTTCCACAAAAGTTCCATTTTCA

>TRINITY_DN44238_c2_g1_i26 from sample A9 / Example unspliced isoform:

CCGTTGGAGAAGTTTCATTTCACGGGAACAAGCGGTACACCGAGTACTGTTTCCAAATCTTCGTAAGTTACATTCATGACCTCCTTGAAATCGAGGGCTGTTTCAGAAGTTTCATTTCACACTCTCGTTTTTCGCAGTGCGGAGACTTAGAAAGGTTTTCACTGCACAAATAAATCCATCTCATCTTGCGGTAGAGCAAT
GGCGCGATTTCAACACAGAGAAATCCGTTTTTGTCCATCTCATCTTGTGGTAGGgcaatggagtgatttcagcatagagaagtccggttttgtccatcttatcttgcggtagggcaatggagcgatttcaacatagGCAATGGAGCGATTTTGTCCATCTCATCTTGTGGTAGGCAATGGAGCGATTTCAACACAGAGGA
ATCCGTTTTTGTCCTTCTCATCTTGTGGTAGGGGAATGGAGCGATTTCAGCACAGGGAAATCTGTATGATTTTCGTTGTGCGGAGACATACAAAGTCTTCAAAATCACGACGAGTTTTTGTCCATCTCATCTTGTGGTAGGGCAATGGAGCGATTTCAGCACAGAGAAATCACGACATCATTGAAAATTGTCGGAAATTG
TTTATCAAGAAAGGGGTTGTTCTCTGCCCCTCATTTTCACTTCTCAGCCGGAGAAACCCTGAAGAAGCAGTACTCCTGCGGTAGGGCAATGTATCGATTTCAGCACAGTGAATGTCTTCAAATCACGATGGCATGGCATCATTGGAAAGTGTGGGAAATTGTTTATGAAGTGAGGGTTTGTGGTAGGGCAATGTAGCGAT
TTCAGCACGGAGAATGTTTTCAAAATCACGACGGTACGACATCATTGAAAATCGTCGAAATTGTTTATCAAGAAAGGGGTTGTTCTCTGCCCCTCAAAATTGTTTATCAAGAAAGGGTTTATTCTCTGCCCCTCGTTTCCAGTTCTCAGCCGGAGAAGCCCTGAAGAAGCGGTACACCGAGGGTTGTTTCCAAATCTGCG
CTATCTTGGTCAGGGCATATCCTCTGGAAGTACATTCATATGCTCTTTAGTCAATGGCTTCAGTATTTCAGGTATACAACTTAATTGGCTTGCATCAGCAGATTTTTCACAACCATTGGTCATGGACAACAACAGGTATGCAATTCTAGAAATATTCCTCTAGCCAAAGTTCCACAAAAGTTCCATTTTCACAAGGAAGA
GTTATTTTCATACGGAAGAGTTATTTCTGACTCAT

Intron 3 GTATACAACT ... ACAACAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.96	24	63	30
A2	80.95	12	65	37
A3	72.41	20	62	43
A4	78.1	15	63	44
A5	73.91	6	21	13
A6	74.49	31	114	67
A7	62.45	52	118	55
A8	68.24	47	124	78
A9	70.08	38	109	69
A10	70.37	28	79	54

>TRINITY_DN44238_c2_g1_i3 from sample A9 / Example spliced isoform

CCGTTGGAGAAGTTTCATTTCACGGGAACAAGCGGTACACCGAGTACTGTTTCCAAATCTTCGTAAGTTACATTCATGACCTCCTTGAAATCGAGGGCTGTTTCAGAAGTTTCATTTCACACTCTCGTTTTTCGCAGTGCGGAGACATACAAAGGTTTTCACTGCACAAATCCGTCTCATTTGCGGTTGAGCAATGGCGC
GATTTCAGCACAGAGAAATCCGTTTTTGTCCATCTCATCTTGTGGTAGGGCAATGGAGCGATTTTGTCCATCTCATCTTGTGGTAGGCAATGGAGCGATTTCAACAGAGGAATCCGTTTTTGTCCTTCTCATCTTGTGGTAGGGGAATGGAGCGATTTCAGCACAGGGAAATCTGTATGATTTTCGTTGTGCGGAGACAT
ACAAAGTCTTCAAAATCACGACGACACGACATCATTGAAAATTGTCGGAAATTGTTTATCAAGAAAGGGGTTGTTCTCTGCCCCTCATTTTCACTTCTCAGCCGGAGAAACCCTGAAGAAGCAGTACTCCTGCGGTAGGGCAATGTATCGATTTCAGCACAGTGAATGTCTTCAAATCACGATGGCATGGCATCATTGGA
AAGTGTGGGAAATTGTTTATGAAGTGAGGGTTTGTGGTAGGGCAATGTAGCGATTTCAGCACGGAGAATGTTTTCAAAATCACGACGGTACGACATCATTGAAAATCGTCGAAATTGTTTATCAAGAAAGGGGTTGTTCTCTGCCCCTCAAAATTGTTTATCAAGAAAGGGTTTATTCTCTGCCCCTCGTTTCCAGTTCT
CAGCCGGAGAAGCCCTGAAGAAGCGGTACACCGAGGGTTGTTTCCAAATCTGCGCTATCTTGGTCAGGGCATATCCTCTGGAAGTACATTCATATGCTCTTTAGTCAATGGCTTCAGTATTTCAGGTATGCAATTCTAGAAATATTCCTCTAGCCAAAGTTCCACAAAAGTTCCATTTTCA

>TRINITY_DN44238_c2_g1_i26 from sample A9 / Example unspliced isoform:

CCGTTGGAGAAGTTTCATTTCACGGGAACAAGCGGTACACCGAGTACTGTTTCCAAATCTTCGTAAGTTACATTCATGACCTCCTTGAAATCGAGGGCTGTTTCAGAAGTTTCATTTCACACTCTCGTTTTTCGCAGTGCGGAGACTTAGAAAGGTTTTCACTGCACAAATAAATCCATCTCATCTTGCGGTAGAGCAAT
GGCGCGATTTCAACACAGAGAAATCCGTTTTTGTCCATCTCATCTTGTGGTAGGGCAATGGAGTGATTTCAGCATAGAGAAGTCCGGTTTTGTCCATCTTATCTTGCGGTAGGGCAATGGAGCGATTTCAACATAGGCAATGGAGCGATTTTGTCCATCTCATCTTGTGGTAGGCAATGGAGCGATTTCAACACAGAGGA
ATCCGTTTTTGTCCTTCTCATCTTGTGGTAGGGGAATGGAGCGATTTCAGCACAGGGAAATCTGTATGATTTTCGTTGTGCGGAGACATACAAAGTCTTCAAAATCACGACGAGTTTTTGTCCATCTCATCTTGTGGTAGGGCAATGGAGCGATTTCAGCACAGAGAAATCACGACATCATTGAAAATTGTCGGAAATTG
TTTATCAAGAAAGGGGTTGTTCTCTGCCCCTCATTTTCACTTCTCAGCCGGAGAAACCCTGAAGAAGCAGTACTCCTGCGGTAGGGCAATGTATCGATTTCAGCACAGTGAATGTCTTCAAATCACGATGGCATGGCATCATTGGAAAGTGTGGGAAATTGTTTATGAAGTGAGGGTTTGTGGTAGGGCAATGTAGCGAT
TTCAGCACGGAGAATGTTTTCAAAATCACGACGGTACGACATCATTGAAAATCGTCGAAATTGTTTATCAAGAAAGGGGTTGTTCTCTGCCCCTCAAAATTGTTTATCAAGAAAGGGTTTATTCTCTGCCCCTCGTTTCCAGTTCTCAGCCGGAGAAGCCCTGAAGAAGCGGTACACCGAGGGTTGTTTCCAAATCTGCG
CTATCTTGGTCAGGGCATATCCTCTGGAAGTACATTCATATGCTCTTTAGTCAATGGCTTCAGTATTTCAGgtatacaacttaattggcttgcatcagcagatttttcacaaccattggtcatggacaacaacagGTATGCAATTCTAGAAATATTCCTCTAGCCAAAGTTCCACAAAAGTTCCATTTTCACAAGGAAGA
GTTATTTTCATACGGAAGAGTTATTTCTGACTCAT

Intron 4 ATGCTCTTTG ... CATCAAAAAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.64	35	45	24
A2	38.36	45	15	41
A3	43.36	32	27	22
A4	41.46	36	25	26
A5	35	26	11	17
A6	31.17	85	33	44
A7	27.17	63	25	22
A8	40	60	48	32
A9	37.35	52	37	25
A10	28.95	27	11	11

>TRINITY_DN44238_c2_g1_i12 from sample A9 / Example spliced isoform

ATGTGAAGTTAAATCATAGTTTGAGCTTCGACTACAGTGCAATTGCATAAAAAACATGCTCTTTGTGCACTTGTATCTTGTTCTCTGCGAATCTTGGGGCTGCAATATTTTGCCAAGAAGAATATCTAAAAATGGATTTGATCTCAAGGTTGTGCTGAAAGAGGTGCCCAGATTCGACAAATGTCCTCACTGTTGTTCAA
AATTCCAATATTATTTCTTCTGGGTGCATCTAATCTCTTCAATTATCAAGCTGGGTCATCGTCCAGGTGGGGTGCATCAGTTAAGTCATCATTTCCATGAACTGCAGAAGTTTCCTCACACATGAACTTGTGTTCTAGGTATTTTTGGCTCTAAGAATTTGAACACAAGTTCCTCACTTTCCAGTTTCTGTCACTAAATC
TACCCCAATCATGTTAGTTGACATAACCTCTTTGATGATATGATCCCATGCTTCAAGAATTTGAACACAAGTTCCTCACTTGCCAGTTTCTGTCACTAAATCTATCCCAATCATGTTAGTTGACTACTATTTCTTTTTTGCGGGTTACATGTTGCACTCCAAGTATGTGAAGTTAAATCATAGTTTGAGCTTCGACTACA
GTGCAATTGCATAAAAAACATGCTCTTTGTGCACTTGTATCTTGTTCTCTGCTAATCTTGGGGCTGCAATATTCTGCCAAGAAGAATATCTAAAAATGGATTTGATCTCAAGGTTGTGCTGAAAGAGGTGCCCAGATTCGACAAATGTCCTCACTGTTGTTCAAAATTCCAATATTATTTCTTCTGGGTGCA

>TRINITY_DN44238_c2_g1_i7 from sample A9 / Example unspliced isoform:

ACCGAGGGTTGTTTCCAAATCTGCGCTATCTTGGTCAGGGCAGATCATCTGGAAGTTGGTCAGGGCAGATCATCTGGAAGTACATTCATATGCTCTTTAGTCAATGGCTTCAGTATTTCAGGTATACAATTTAATTGGCTTGAATCAGCAGATTTTTCACAACCATTGGTCATGGACAACAACAGGTTCTTCTTTGTCAT
TGACCTCAATATATTCTTATATTGCCATGACATGGGTGTCTACTATTTCTTTTTTGCGGGTTACATGTTGCACTCCAAGTATGTGAAGTTAAATCATAGTTTGAGCTTCGACTACAGTGCAATTGCATAAAAAACatgctctttgtgcactgaattgcatcaaaaacATGCTCTTTGTGCACATGTATCTTGTTCTCTGC
GAATCTTGGGGCTGCAATATTTTGCCAAGAAGAATATCTAAAAATGGATTTGATCTCAAGGTTGTGCTGAAAGAGGTGCCCAGATTCGACAAATGTCCTCACTGTTGTTCAAAATTCCAATATTATTTCTTCTGGGTGCATCTAATCTCTTCAATTATCAAGCTGGGTCATCGTCCAGGTGGGGTGCATCAGTTAAGTCA
TCATTTCCATGAACTGCAGAAGTTTCCTCACACATGAACTTGTGTTCTAGGTATTTTTGGCTCTAAGAATTTGAACACAAGTTCCTCACTTTCCAGTTTCTGTCACTAAATCTACCCCAATCATGTTAGTTGACATAACCTCTTTGATGATATGATCCCATGCTTCAAGAATTTGAACACAAGTTCCTCACTTGCCAGTT
TCTGTCACTAAATCTATCCCAATCATGTTAGTTGACTACTATTTCTTTTTTGCGGGTTACATGTTGCACTCCAAGTATGTGAAGTTAAATCATAGTTTGAGCTTCGACTACAGTGCAATTGCATAAAAAACATGCTCTTTGTGCACTTGTATCTTGTTCTCTGCTAATCTTGGGGCTGCAATATTCTGCCAAGAAGAATA
TCTAAAAATGGATTTGATCTCAAGGTTGTGCTGAAAGAGGTGCCCAGATTCGACAAATGTCCTCACTGTTGTTCAAAATTCCAATATTATTTCTTCTGGGTGCA