NA ----> NA

Intron 1 GTATGTACGG ... TGTAGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.32	211	278	185
A2	43.54	166	171	85
A3	42.86	182	173	100
A4	46.69	133	153	80
A5	34.68	129	84	53
A6	24.44	473	216	90
A7	30.62	921	575	238
A8	28.53	1052	613	227
A9	27.08	618	325	134
A10	27.88	666	356	159

>TRINITY_DN128158_c3_g1_i2 from sample A1 / Example spliced isoform

TTTGGGAATTATTTGAATTTCAGAGTCTTTTTGAGTTTTTTTTTTCTTCTTCTTAGATTAAGCATGGCCACGCTTTTATTTGTTTCTACTTGTCAATATGTTTGTGTTTTCTTCTGCTTTTTCATTTTTAATTGTTTAGTTTTAATGTGATTATGTTTTGTTTGTATCTTTTCGTTTATGATTTTTTGTCTTGTTTTGGA
TAGAAAAGATACTGCCACAGAAAACTGAATATGAGAGCTCATGAGGAGGAACGCTAGCAAGTGAATTTCAGCCACCGCCGAATTTGAAGATAGAATTCGCATAAACATCTACACATAGAGTTTGGATATGTTAAGTT

>TRINITY_DN128158_c3_g1_i1 from sample A1 / Example unspliced isoform:

TTTGGGAATTATTTGAATTTCAGAGTCTTTTTGAGTTTTTTTTTTCTTCTTCTTAGATTAAGCATGGCCACGCTTTTATTTGTTTCTACTTGTCAATATGTTTGTGTTTTCTTCTGCTTTTTCATTTTTAATTGTTTAGTTTTAATGTGATTATGTTTTGTTTGTATCTTTTCGTTTATGACTTTTTGTCTCGTTTTGGA
GAGAAAAGATACTGCCACAGAAAACTGAATATGAGAGCTCATGAGgtatgtacggttttaatttctgaacaacactgtgcttttaattcttgtttgagtaatattttctgctttgttgtgtagtgtagGAGGAACGCTAGCAAGTGAATTTCAGCCACCGCCGAATTTGAAGATAGAATTCGCATAAACATCTACACATA
GAGTTTGGATATGTTAAGTT

Intron 2 GTATGTACGG ... TGTAGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.9	211	194	88
A2	45.21	166	137	33
A3	42.95	182	137	47
A4	48.45	133	125	43
A5	36.14	129	73	20
A6	28.12	473	185	49
A7	36.79	921	536	125
A8	36.01	1052	592	111
A9	30.56	618	272	58
A10	30.7	666	295	70

>TRINITY_DN128158_c3_g1_i2 from sample A1 / Example spliced isoform

TTTGGGAATTATTTGAATTTCAGAGTCTTTTTGAGTTTTTTTTTTCTTCTTCTTAGATTAAGCATGGCCACGCTTTTATTTGTTTCTACTTGTCAATATGTTTGTGTTTTCTTCTGCTTTTTCATTTTTAATTGTTTAGTTTTAATGTGATTATGTTTTGTTTGTATCTTTTCGTTTATGATTTTTTGTCTTGTTTTGGA
TAGAAAAGATACTGCCACAGAAAACTGAATATGAGAGCTCATGAGGAGGAACGCTAGCAAGTGAATTTCAGCCACCGCCGAATTTGAAGATAGAATTCGCATAAACATCTACACATAGAGTTTGGATATGTTAAGTT

>TRINITY_DN128158_c3_g1_i4 from sample A1 / Example unspliced isoform:

TTTGGGAATTATTTGAATTTCAGAGTCTTTTTGAGTTTTTTTTTTCTTCTTCTTAGATTAAGCATGGCCACGCTTTTATTTGTTTCTACTTGTCAATATGTTTGTGTTTTCTTCTGCTTTTTCATTTTTAATTGTTTAGTTTTAATGTGATTATGTTTTGTTTGTATCTTTTCGTTTATGACTTTTTGTCTCGTTTTGGA
GAGAAAAGATACTGCCACAGAAAACTGAATATGAGAGCTCATGAGgtatgtacggttttaatttcttaacaacactgtgctttatccatggaatacattatagggttttaatttctgaacaacactgtgcttttaattcttgtttgagtaatattgtctgctttgttgtgtagtgtagGAGGAACGCTAGCAAGTGAATT
TCAGCCACCGCCGAATTTGAAGATAGAATTCGCATAAACATCTACACATAGAGTTTGGATATGTTAAGTT

Intron 3 GTATGTACGA ... TGTAGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.48	236	305	158.5
A2	50.41	193	194.5	74
A3	48.08	211	193.5	83
A4	52.45	150.5	165	64
A5	45.08	136	111.5	47
A6	31.57	544.5	248	69.5
A7	42.3	1004.5	733.5	191.5
A8	39.63	1185.5	772	190.5
A9	35.7	700	385	106.5
A10	37	745.5	434.5	130.5

>TRINITY_DN128158_c3_g1_i2 from sample A1 / Example spliced isoform

TTTGGGAATTATTTGAATTTCAGAGTCTTTTTGAGTTTTTTTTTTCTTCTTCTTAGATTAAGCATGGCCACGCTTTTATTTGTTTCTACTTGTCAATATGTTTGTGTTTTCTTCTGCTTTTTCATTTTTAATTGTTTAGTTTTAATGTGATTATGTTTTGTTTGTATCTTTTCGTTTATGATTTTTTGTCTTGTTTTGGA
TAGAAAAGATACTGCCACAGAAAACTGAATATGAGAGCTCATGAGGAGGAACGCTAGCAAGTGAATTTCAGCCACCGCCGAATTTGAAGATAGAATTCGCATAAACATCTACACATAGAGTTTGGATATGTTAAGTT

>TRINITY_DN128158_c3_g1_i3 from sample A1 / Example unspliced isoform:

TTTGGGAATTATTTGAATTTCAGAGTCTTTTTGAGTTTTTTTTTTCTTCTTCTTAGATTAAGCATGGCCACGCTTTTATTTGTTTCTACTTGTCAATATGTTTGTGTTTTCTTCTGCTTTTTCATTTTTAATTGTTTAGTTTTAATGTGATTATGTTTTGTTTGTATCTTTTCGTTTATGATTTTTTGTCTTGTTTTGGA
TAGAAAAGATACTGCCACAGAAAACTGAATATGAGAGCTCATGAGgtatgtacgattttaatttcttaacaacactgtgctttatccatggaatacattatagggttttgagtaatattttctgctttgttgtgtagtgtagGAGGAACGCTAGCAAGTGAATTTCAGCCACCGCCGAATTTGAAGATAGAATTCGCATA
AACATCTACACATAGAGTTTGGATATGTTAAGTT

Intron 4 GTATGTACCA ... TGTATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	59	96
A2	100	0	32	59
A3	100	0	37	48
A4	100	0	29	46
A5	100	0	14	16
A6	100	0	35	52
A7	100	0	127	241
A8	100	0	192	291
A9	100	0	47	67
A10	100	0	74	149

>TRINITY_DN121464_c2_g2_i7 from sample A2 / Example spliced isoform

TTTGAATTTCAGAGTCTTTTTGAGCGTTTTTTTCTCTTCTTCTTAGATTAAGCATGGCCACGCTTTTATTTGTTTCTACTTGTCAATATGTTTGTGTTTTCTTTTAGTTTTTCATTTATAATTGTTTAGTTTTAATTTGATTATGTTTTGTTTGCATCTTTTCGTTTATGATTTTTTGTTTTGTTTGGGAGAGAAAAGAT
GCTGCTACAGAAAACTGAATATGGCTTTTTCATTTTTAATCGTTTAGTTTTAATGTGATTATGTTTTGTTTGTATCTTTTCGTTTATGAAGAGCTGATAAGGAGGGGAACGCTAGCAGGTGAATTTCAGCCACCGCCGAATTTCCGAATTTGAAAATAGCAGATGCTGCTACATAAAACTGAA

>TRINITY_DN121464_c2_g2_i9 from sample A2 / Example unspliced isoform:

TTTGAATTTCAGAGTCTTTTTGAGCGTTTTTTTCTCTTCTTCTTAGATTAAGCATGGCCACGCTTTTATTTGTTTCTACTTGTCAATATGTTTGTGTTTTCTTTTAGTTTTTCATTTATAATTGTTTAGTTTTAATTTGATTATGTTTTGTTTGCATCTTTTCGTTTATGATTTTTTGTTTTGTTTGGGAGAGAAAAGAT
GCTGCTACAGAAAACTGAATATGGCTTTTTCATTTTTAATCGTTTAGTTTTAATGTGATTATGTTTTGTTTGTATCTTTTCGTTTATGAAGAGCTGATAAGgtatgtaccattttaatttcttaacaacactgtgctttgtccatggaatacattatagggttcgttgttactatttaatttttgttttagtaatatttt
cttctttgtcgtgtattgtagGAGGGGAACGCTAGCAGGTGAATTTCAGCCACCGCCGAATTTCCGAATTTGAAAATAGCAGATGCTGCTACATAAAACTGAA