NA ----> NA

Intron 1 GTATTGTGGT ... TTCTCTCCAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40	3	1	3
A2	-1	0	0	0
A3	0	2	0	0
A4	100	0	2	0
A5	100	0	0	1
A6	33.33	4	4	0
A7	14.29	3	0	1
A8	23.08	5	1	2
A9	16.67	5	2	0
A10	-1	0	0	0

>TRINITY_DN42651_c1_g2_i5 from sample A6 / Example spliced isoform

CAAGATTGAGATATTGTCAAAGAAAGAAGCGGTTATCACATGCAAATGATAGTAGCATTCAATTGCGAATGAAGCGGATCAATGAGGTTTTAAAACCGAGAAAGCTGAGTATGATCTAACCATTTCATGGAAGATAAAACATAGAGATGAAATCGCTGGGAATAAGGAGATTGTATTCTGCATACGAGAAGGGGGATTTT
CTATCTTTTCACTATTTACAGTTTACATTACTAGAATTTTAACAACATTCCACCTTCGTAAATGAACGCTGATTGATATAACTTTTGCAAGTTTGCAGGGATGCTAAGCCATGGAGGTTACGATATCAATGAGGATAACATCATCGAAGAAAAGAGAGGGAACGTTACTGTGATTTGGCAGAATCATCGAGATAATCTCA
ATCGTTCGCATAAAATGAAGTTCCAAAAAATTACTTGCTCAATGATCCCCATCTTCCTACTATTTTGTTAACAATGCGATTACTTATTATTATCTGTCATTATAAGTGAAAGCGGAAATGGCAAAGATACGATGAAACCGTTGCAGGTAAGTCTCGTGGAATATGACACGTCAATTCAATAGAAGGAAATAAGGATGATA
GAGAAATGTTCAACAGAAACTATGAACCAGCTCATCAAATGAAAGCAGAGATCGTGGAGGAGTTCCACTAAAGAAGCAAAGCTCAGTCATAGTTTGTCTACGTGCCTCAATTTCCAGAAAAGGAGGCAGGATAGAATGATCGGTGTTCATCCCCATCATATCACTCATAATGAAGAAAGTTCGTTTTGTAGATTTGACAG
ATGATGCGTGTACATATTTCTGAGAAGCCAGCTATTAAGAGAACTTAATGCGACTGAAAAGGAGAAAGTATGTTCTTCTAATGGAAATGGGATTCATTACATAGCTTATTTTAGTAGCATAGAATCGACTGTCTTTGGGGGGGCGGGTTTCTCAGCAGAATAGAGAGGACTTTGTTATTCTCAAGTTATGTTTCTGTTTA
TAAATCTTTTATGTTTATAGATAGGAGGGGAGACGCTGCCAGAGAGGAGAGAGCTCTGCAGTTTTGATGGCCTTTACCTGGGAACTGTGGATATTCATTTTTCTGTAATGTTTATTCCAGAAGAAGAATCAGTCATCTTCTCTGCCACTGTTTTTGGATATCGATGGAAGCCTTGTGTTGTTTCTACCCAGACATAATTT
GAGACCAGAGATTAGTGATTGTGCAATCATCTTCGTACTTGATTGGGATCTGTAAGATTTTATTTTTTACAG

>TRINITY_DN42651_c1_g2_i9 from sample A6 / Example unspliced isoform:

CAAGATTGAGATATTGTCAAAGAAAGAAGCGGTTATCACATGCAAATGATAGTAGCATTCAATTGCGAATGAAGCGGATCAATGAGGTTTTAAAACCGAGAAAGCTGAGTATGATCTAACCATTTCATGGAAGATAAAACATAGAGATGAAATCGCTGGGAATAAGGAGATTGTATTCTGCATACGAGAAGGGGGATTTT
CTATCTTTTCACTATTTACAGTTTACATTACTAGAATTTTAACAACATTCCACCTTCGTAAATGAACGCTGATTGATATAACTTTTGCAAGTTTGCAGGGATGCTAAGCCATGGAGGTTACGATATCAATGAGGATAACATCATCGAAGAAAAGAGAGGGAACGTTACTGTGATTTGGCAGAATCATCGAGATAATCTCA
ATCGTTCGCATAAAATGAAGTTCCAAAAAATTACTTGCTCAATGATCCCCATCTTCCTACTATTTTGTTAACAATGCGgtattgtggtggattttatattttaaatggatatcttcacgaatttaattttcttgcattttattttaacatatgagctagattaagcttggtttagattttgtttacaaagcatcaaaatt
gagcaatcttaataatggttattctcacggcttctctccagATTACTTATTATTATCTGTCATTATAAGTGAAAGCGGAAATGGCAAAGATACGATGAAACCGTTGCAGGTAAGTCTCGTGGAATATGACACGTCAATTCAATAGAAGGAAATAAGGATGATAGAGAAATGTTCAACAGAAACTATGAACCAGCTCATCA
AATGAAAGCAGAGATCGTGGAGGAGTTCCACTAAAGAAGCAAAGCTCAGTCATAGTTTGTCTACGTGCCTCAATTTCCAGAAAAGGAGGCAGGATAGAATGATCGGTGTTCATCCCCATCATATCACTCATAATGAAGAAAGTTCGTTTTGTAGATTTGACAGATGATGCGTGTACATATTTCTGAGAAGCCAGCTATTA
AGAGAACTTAATGCGACTGAAAAGGAGAAAGTATGTTCTTCTAATGGAAATGGGATTCATTACATAGCTTATTTTAGTAGCATAGAATCGACTGTCTTTGGGGGGGCGGGTTTCTCAGCAGAATAGAGAGGACTTTGTTATTCTCAAGTTATGTTTCTGTTTATAAATCTTTTATGTTTATAGATAGGAGGGGAGACGCT
GCCAGAGAGGAGAGAGCTCTGCAGTTTTGATGGCCTTTACCTGGGAACTGTGGATATTCATTTTTCTGTAATGTTTATTCCAGAAGAAGAATCAGTCATCTTCTCTGCCACTGTTTTTGGATATCGATGGAAGCCTTGTGTTGTTTCTACCCAGACATAATTTGAGACCAGAGATTAGTGATTGTGCAATCATCTTCGTA
CTTGATTGGGATCTGTAAGATTTTATTTTTTACAG

Intron 2 GTAAAGACCC ... CCTTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.58	15	45	43
A2	65.85	14	27	27
A3	55.56	22	29	26
A4	77.53	10	39	30
A5	60	3	7	2
A6	63.19	67	130	100
A7	65.18	117	221	217
A8	71.63	100	240	265
A9	65.06	87	155	169
A10	63.14	61	111	98

>TRINITY_DN121956_c1_g1_i5 from sample A1 / Example spliced isoform

GGCCGGAAGCTCAACGATAATTACATCTGCAGAATATGTGGTAAATCAGTAAAACTAAAAGATATGAAGTTGCAATATGATCTATCTTGGAGAAGCCTTCGCAATTTTGTGAAACGACCACAATCATTGAGAATATGAAACTGCAGAAGTATTGCCATTAGACCCGCATAAGTTTCCTTGGTAAAAGATAAACGCATATA
CCAGTAAGATAATGCCTGGGGATATCTTAACCAAAATGTGCCAGTAAAAGAAGATTTCACGCGGAAGAATCAAAAGTAAAGAGTTGGAGATCGTGGAGGAGTTCCACTAAAGAAGCAAAGCTCAGTTACAGTTTGTCTACGTGCCTCAATTTTCAGAAAAGGAGGCAGGATAGAATGATCGGTGTTCATCCTTATCATAT
CACTCAGAATGAAGAAAAGTTCGTTTTGTAGATTCGACAGATGATGCGTGTACATATTTCTGAGAAGCCAGCTATTAAGAGAACTTAATGCGACTGAAAAGGAGAAAGTATGTTCTTCTAATGGAAATGGGATTCATTACATAGCTTATTTTAGTAGCATAGAATCGACTGTCTTTGGGGGGGCGGGTTTCTCAGCAGAA
TAGAGAGGACTTTGTTATTCTCA

>TRINITY_DN121956_c1_g1_i2 from sample A1 / Example unspliced isoform:

GGCCGGAAGCTCAACGATAATTACATCTGCAGAATATGTGGTAAATCAGTAAAACTAAAAGATATGAAGTTGCAATATGATCTATCTTGGAGAAGCCTTCGCAATTTTGTGAAACGACCACAATCATTGAGAATATGAAACTGCAGAAGTATTGCCATTAGACCCGCATAAgtaaagaccctgccagagaaagtcggaat
tcgactcgatgcgttactgaagaaaatgcagaatcttttaaaaagatcgttggaaatttactatgaaggaagtattattgtttcatttatctttatggttttgatttataataaaatgatattctgtctccttatgcagGTTTCCTTGGTAAAAGATAAACGCATATACCAGTAAGATAATGCCTGGGGATATCTTAACC
AAAATGTGCCAGTAAAAGAAGATTTCACGCGGAAGAATCAAAAGTAAAGAGTTGGAGATCGTGGAGGAGTTCCACTAAAGAAGCAAAGCTCAGTTACAGTTTGTCTACGTGCCTCAATTTTCAGAAAAGGAGGCAGGATAGAATGATCGGTGTTCATCCTTATCATATCACTCAGAATGAAGAAAAGTTCGTTTTGTAGA
TTCGACAGATGATGCGTGTACATATTTCTGAGAAGCCAGCTATTAAGAGAACTTAATGCGACTGAAAAGGAGAAAGTATGTTCTTCTAATGGAAATGGGATTCATTACATAGCTTATTTTAGTAGCATAGAATCGACTGTCTTTGGGGGGGCGGGTTTCTCAGCAGAATAGAGAGGACTTTGTTATTCTCA