sp|Q84WS8|U1125_ARATH ----> U11/U12 small nuclear ribonucleoprotein 25 kDa protein

Intron 1 GTATCATTTA ... CTTCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.8	32.5	8.4	1.4
A2	37.68	18.1	10.9	4
A3	36.3	46.6	26.4	8.8
A4	16.62	25	5.2	0
A5	21.45	6	1.6	0.3
A6	13.52	99.7	15.5	1.1
A7	11.9	147	19.7	0
A8	9.62	140.8	15.1	0.3
A9	15.11	167.5	29.8	0.3
A10	14.47	140.8	23.7	0.5

>TRINITY_DN104417_c1_g1_i1 from sample A2 / Example spliced isoform

ATGAAATCCATAGAAGCAGCTGCACTGGGGGCTGGTGATGAATCACCCCTGTTATCTAAAGAAGAAGAGTGCACTTCTGGTTCAATTCGCCTATGTATTTTCAGAATGGATAATACATCGTTTGGTAAGTTTTGAGCATAATATGAAATTTAACTTTGGAGTTGCAGATGGGTTGTTGAGAACTATTGGCTGAATTCTAC
TACAGATGTAAGTGTGCCTGGAACAGCTTCTGTTCTTAAACTAAAGAAGGCCATCGAGGAAAAGTTCGGTGACTCGCAGATGGAGGAAGACACAATTTCCTGGTGTCATGTCTGGGGAAACTTCTGCTTGACCTACCAAGATCAAGAGCTTATTAATGACGGGTCGTTGTTGAGCAGTTTTGGTATCAAGGACAGCGACG
AGCTTTTTTTTGTACGGCATATGTCTTCAGCAAGTCAAGGGGAGAAACTTCATCGAAGAAAACATGGCACATTTGGAGTAATGAAAAAGAAAACATGGCACATTTGGAG

>TRINITY_DN104417_c1_g1_i5 from sample A2 / Example unspliced isoform:

ATGAAATCCATAGAAGCAGCTGCACTGGGGGCTGGTGATGAATCACCCCTGTTATCTAAAGAAGAAGAGTGCACTTCTGGTTCAATTCGCCTATGTATTTTCAGAATGGATAATACATCGTTTGATGTAAGTGTGCCTGGAACAGCTTCTGTTCTTAAACTAAAGAAGGCCATCGAGGAAAAGTTCGGTGACTCGCAGAT
GGAGGAAGACACAATTTCCTGgtatcatttattcacagccatactgcattttgtttttctgtcttaggaattgttgctgttgttgggttttgagtttgaagaattgggtgcttcttgcagGTGTCATGTCTGGG

Intron 2 GTATGGACTT ... TATCTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.85	46.2	23.4	16
A2	30.4	38.5	15.6	18
A3	33.33	33.2	17.2	16
A4	25.42	22	4	11
A5	27.03	26.7	14.8	5
A6	25.42	203.4	84.6	54
A7	24.93	281.5	118	69
A8	30.22	207	121.4	58
A9	24.08	309.9	124.8	72
A10	19.56	266.3	79.8	50

>TRINITY_DN104417_c1_g1_i7 from sample A2 / Example spliced isoform

ATGAAATCCATAGAAGCAGCTGCACTGGGGGCTGGTGATGAATCACCCCTGTTATCTAAAGAAGAAGAGTGCACTTCTGGTTCAATTCGCCTATGTATTTTCAGAATGGATAATACATCGTTTGATGTAAGTGTGCCTGGAACAGCTTCTGTTCTTAAACTAAAGAAGGCCATCGAGGAAAAGTTCGGTGACTCGCAGAT
GGAGGAAGACACAATTTCCTGGTGTCATGTCTGGGGAAACTTCTGCTTGACCTACCAAGATCAAGAGCTTATTAATGACGGGTCGTTGTTGAGCAGTTTTGGTATCAAGGACAGCGACGAGCTTTTTTTTGTACGGCATATGTCTTCAGCAAGTCAAGGGGAGAAACTTCATCGAAGAAAACATGGCACATTTGGAGTAA
TGAAAAAGAAGTCTTCTGATGGTGCAAATATGAATGGACGCAAAGCCTTTACCCAAATTGAGCTCCATTGGACACGCACCATAAGAACGTGGTTTGTCTATACAAAACTTTGGATTATTGGTATGAAAGCAGATTCCAAGAGAATACAACTAAGGCACTGAAACATGACAAGTGACAAATCAAATGTTCGAGAGATGTGT
ATATATATATATATATATATATATTTAAATCAGTAATTCACCTGCTTCCCTGAAATAATATATATTTATGAAGAATTGTTTCACTCCACTCCAATGAAACACAGGAGTACTGTTGATAGGATGGCTTAAAATTGTAGAACCTAAATTCCACCACCTTGTTAGCTCTAAGAGGTGATACAATTTTCAGGAGACACGACACT
CTGGGTCCCATTCTTTGAAATGGCATGAGCCAGATGTAAACTAGTTCTTTAGATATTGACAGATAGGATTACAAAGAGCTGGGATGGGTCACTGAAATTATGACCATTCATCAATGGTATGGAAATTTTTATTAAAGGTGTCACTATCTATCAAGTTTGTAGTGGTTTGGAGTTTCGTGACTGCAACAGCGAATGTATCT
ACTCAAGATATAAATTTTAGCTTGTACAGTACATTAAGCATTTGGTGGTATATCCAGACTAGAAACCAACAAAAGGACTTTCCTTTTTGTTTGATTACAGTTGCAATCTTCATCTGAGGAACACTCCAGTTTCATGTACTATTTTGACTACTCTCTTGACATTAATTATATTTATTCTTGTTCAATGTTGAAAGTAAAAG
GTTGGAAGTCCATTGTGCTGACAAGGTGCACATTTCACTGATACCGTTAAGATTTGAATCT

>TRINITY_DN104417_c1_g1_i3 from sample A2 / Example unspliced isoform:

ATGAAATCCATAGAAGCAGCTGCACTGGGGGCTGGTGATGAATCACCCCTGTTATCTAAAGAAGAAGAGTGCACTTCTGGTTCAATTCGCCTATGTATTTTCAGAATGGATAATACATCGTTTGATGTAAGTGTGCCTGGAACAGCTTCTGTTCTTAAACTAAAGAAGGCCATCGAGGAAAAGTTCGGTGACTCGCAGAT
GGAGGAAGACACAATTTCCTGGTGTCATGTCTGGGGAAACTTCTGCTTGACCTACCAAGATCAAGAGCTTATTAATGACGGGTCGTTGTTGAGCAGTTTTGGTATCAAGGACAGCGACGAGCTTTTTTTTGTACGGCATATGTCTTCAGCAAGTCAAGGGGAGAAACTTCATCGAAGAAAACATGGCACATTTGGAGTAA
TGAAAAAGAAgtatggacttggtgacttgttttttcattattgtttgtccattattttgtattgtcaataattcctgatcttcttcctctatcaaaatatatggccaatcagagtaaccttctatatcagtcttaacaataatttaaattggcatcacaagaatctttagaggaaataattgtcagtaaaggtgaaggat
tatgcatgcattttgaatttttgtatttcatctttgtgataactttgtcgatgtgtggagaatggtaggatgctgcagtgtctttcgaaatctactttaaatatttagctggatttcccctttatgtaccaatatgcccttggtctctcgcattatgattacgagtaattctcattaatggtaaatttttggttgtgtca
tttgttttgtaacttgttgcctctttcattggcttttagttagtgaattattcacattttatttacaaattacttttatgcttctattgagtggaaatttgttacctgatcctatcattttggttttaagaatacttcttaacatcattagttaatcacttttttattgtgcattgtctgtgtattacgttgggggaaat
cgttaggtttgtatgcataattgttgactgcttgcttgaacggacagtttttttgttagtttcagccactgtttaatcataaaaccaatattatatttgatgggtgatgttcagaaatggtataaaaagtaccgtgggctaagttttcaaatctatgaagatcgttctttactcattctaacagattctatctaacagGT
CTTCTGATGGTGCAAATATGAATGGACGCAAAGCCTTTACCCAAATTGAGCTCCATTGGACACGCACCATAAGAACGTGGTTTGTCTATACAAAACTTTGGATTATTGGTATGAAAGCAGATTCCAAGAGAATACAACTAAGGCACTGAAACATGACAAGTGACAAATCAAATGTTCGAGAGATGTGTATATATATATAT
ATATATATATATTTAAATCAGTAATTCACCTGCTTCCCTGAAATAATATATATTTATGAAGAATTGTTTCACTCCACTCCAATGAAACACAGGAGTACTGTTGATAGGATGGCTTAAAATTGTAGAACCTAAATTCCACCACCTTGTTAGCTCTAAGAGGTGATACAATTTTCAGGAGACACGACACTCTGGGTCCCATT
CTTTGAAATGGCATGAGCCAGATGTAAACTAGTTCTTTAGATATTGACAGATAGGATTACAAAGAGCTGGGATGGGTCACTGAAATTATGACCATTCATCAATGGTATGGAAATTTTTATTAAAGGTGTCACTATCTATCAAGTTTGTAGTGGTTTGGAGTTTCGTGACTGCAACAGCGAATGTATCTACTCAAGATATA
AATTTTAGCTTGTACAGTACATTAAGCATTTGGTGGTATATCCAGACTAGAAACCAACAAAAGGACTTTCCTTTTTGTTTGATTACAGTTGCAATCTTCATCTGAGGAACACTCCAGTTTCATGTACTATTTTGACTACTCTCTTGACATTAATTATATTTATTCTTGTTCAATGTTGAAAGTAAAAGGTTGGAAGTCCA
TTGTGCTGACAAGGTGCACATTTCACTGATACCGTTAAGATTTGAATCT

Intron 3 GTAAGTTTTG ... TCTACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.15	36	20.6	26.2
A2	41.14	39.9	30.3	27.9
A3	51.49	36.3	28.4	38.5
A4	52.27	23.4	14.2	25.6
A5	44.62	9.9	16	8
A6	31.12	135.2	66.6	61.1
A7	25.14	239.1	84.2	80.5
A8	23.91	167	77.1	52.7
A9	22.52	250	111.5	72.8
A10	22.44	195.8	87.7	56.7

>TRINITY_DN124155_c0_g1_i6 from sample A1 / Example spliced isoform

CAGCACACCGCCCATTTGCACGGCCAAGATGAAATCCATAGAAGCAGCTGCACTGGGGGCTGGTGATGAATCACCCCTGTTATCTAAAGAAGAAGAGTGCACTTCTGGTTCAATTCGCCTATGTATTTTCAGAATGGATAATACATCGTTTGATGTAAGTGTGCCTGGAACAGCTTCTGTTCTTAAACTAAAGAAGGCCA
TCGAGGAAAAGTTCGGTGACTCGCAGATGGAGGAAGACACAATTTCCTGGTGTCATGTCTGGGGAAACTTCTGCTTGACCTACCAAGATCAAGAGCTTATTAATGACGGGTCGTTGTTGAGCAGTTTTGGTATCAAGGACAGCGACGAGCTTTTTTTTGTACGGCATATGTCTTCAGCAAGTCAAGGGGAGAAACTTCAT
CGAAGAAAACATGGCACATTTGGAGTAATGAAAAAGAAGTCTTCTGATGGTGCAAATATGAATGGACGCAAAGCCTTTACCCAAATTGAGCTCCATTGGACACGCACCATAAGAACGTGGTTTGTCTATACAAAACTTTGGATTATTGGTATGAAAGCAGATTCCAAGAGAATACAACTAAGGCACTGAAACATGACAAG
TGACAAATCAAATGTTCGAGAGATGTGTATATATATATATATATATATATATTTAAATCAGTAATTCACCTGCTTCCCTGAAATAATATATATTTATGAAGAATTGTTTCACTCCACTCCAATGAAACACAGGAGTACTGTTGATAGGATGGCTTAAAATTGTAGAACCTAAATTCCACCACCTTGTTAGCTCTAAGAGG
TGATACAATTTTCAGGAGACACGACACTCTGGGTCCCATTCTTTGAAATGGCATGAGCCAGATGTAAACTAGTTCTTTAGATATTGACAGATAGGATTACAAAGAGCTGGGATGGGTCACTGAAATTATGACCATTCATCAATGGTATGGAAATTTTTATTAAAGGTGTCACTATCTATCAAGTTTGTAGTGGTTTGGAG
TTTCGTGACTGCAACAGCGAATGTATCTACTCAAGATATAAATTTTAGCTTGTACAGTACATTAAGCATTTGGTGGTATATCCAGACTAGAAACCAACAAAAGGACTTTCCTTTTTGTTTGATTACAGTTGCAATCTTCATCTGAGGAACACTCCAGTTTCATGTACTATTTTGACTACTCTCTTGACATTAATTATATT
TATTCTTGTTCAATGTTGAAAGTAAAAGGTTGGAAGTCCATTGTGCTGACAAGGTGCACATTTCACTGATACCGTTAAGATTTGAATCTGCTGCAGGCCTGAAATGATGAACATGAACATCACATATAAGCTAAGCTTGGAGATGAAGTAATGTCAATATTAATAATATATATTAACATTACCTCAAAATGAGGTGGTAC
ATCTAAATTTTAATGGTGATTACTTAAGATAATCT

>TRINITY_DN124155_c0_g1_i7 from sample A1 / Example unspliced isoform:

CAGCACACCGCCCATTTGCACGGCCAAGATGAAATCCATAGAAGCAGCTGCACTGGGGGCTGGTGATGAATCACCCCTGTTATCTAAAGAAGAAGAGTGCACTTCTGGTTCAATTCGCCTATGTATTTTCAGAATGGATAATACATCGTTTGgtaagttttgagcataatatgaaatttaactttggagttgcagatggg
ttgttgagaactattggctgaattctactacagATGTAAGTGTGCCTGGAACAGCTTCTGTTCTTAAACTAAAGAAGGCCATCGAGGAAAAGTTCGGTGACTCGCAGATGGAGGAAGACACAATTTCCTGGTGTCATGTCTGGGGAAACTTCTGCTTGACCTACCAAGATCAAGAGCTTATTAATGACGGGTCGTTGTTG
AGCAGTTTTGGTATCAAGGACAGCGACGAGCTTTTTTTTGTACGGCATATGTCTTCAGCAAGTCAAGGGGAGAAACTTCATCGAAGAAAACATGGCACATTTGGAGTAATGAAAAAGAAGTATGGACTTGGTGACTTGTTTTTTCATTATTGTTTGTCCATTATTTTGTATTGTCAATAATTCCTGATCTTCTTCCTCTA
TCAAAATATATGGCCAATCAGAGTAACCTTCTATATCAGTCTTAACAATAATTTAAATTGGCATCACAAGAATCTTTAGAGGAAATAATTGTCAGTAAAGGTGAAGGATTATGCATGCATTTTGAATTTTTGTATTTCATCTTTGTGATAACTTTGTCGATGTGTGG

Intron 4 GTAAAAGTTT ... ATGAACACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.29	12	2	19
A2	55.56	4	0	5
A3	50	2	0	2
A4	50	1	0	1
A5	45.45	6	2	5
A6	44.9	54	2	44
A7	46.94	78	4	69
A8	47.62	55	3	50
A9	49.31	73	4	71
A10	50.99	74	0	77

>TRINITY_DN42707_c0_g1_i1 from sample A7 / Example spliced isoform

CGGGGGAAAGAAAGATGGTATTTTTCATTGGTCTAAAGTATATATCTAGTGTGTATATATGATATTCTTTTGCATGGACATGGCTTGATATAAAATATAGCACAGCACACCGCCCATTTGCACGGCCAAGATGAAATCCATAGAAGCAGCTGCACTGGGGGCTGGTGATGAATCACCCCTGTTATCTAAAGAAGAAGAGT
GCACTTCTGGTTCAATTCGCCTATGTATTTTCAGAATGGATAATACATCGTTTGATGTAAGTGTGCCTGGAACAGCTTCTGTTCTTAAACTAAAGAAGGCCATCGAGGAAAAGTTCGGTGACTCGCAGATGGAGGAAGACACAATTTCCTGGTGTCATGTCTGGGGAAACTTCTGCTTGACCTACCAAGATCAAGAGCTT
ATTAATGACGGGTCGTTGTTGAGCAGTTTTGGTATCAAGGACAGCGACGAGGAGGACATCCCGAGTTGAATAACTCATCATCCTGAGTTGAAGCATTTCTCCTTACAAATCCTCTTGTAAACAAATAGCTTTTTTTTGTACGGCATATGTCTTCAGCAAGTCAAGGGGAGAAACTTCATCGAAGAAAACATGGCACATTT
GGAGTAATGAAAAAGAAGTCTTCTGATGGTGCAAATATGAATGGACGCAAAGCCTTTACCCAAATTGAGCTCCATTGGACACGCACCATAAGAACGTGGTTTGTCTATACAAAACTTTGGATTATTGGTATGAAAGCAGATTCCAAGAGAATACAACTAAGGCACTGAAACATGACAAGTGACAAATCAAATGTTCGAGA
GATGTGTATATATATATATATATATATATATTTAAATCAGTAATTCACCTGCTTCCCTGAAATAATATATATTTATGAAGAATTGTTTGACTCAACTC

>TRINITY_DN42707_c0_g1_i6 from sample A7 / Example unspliced isoform:

ATGTAAGTGTGCCTGGAACAGCTTCTGTTCTTAAACTAAAGAAGGCCATCGAGGAAAAGTTCGGTGACTCGCAGATGGAGGAAGACACAATTTCCTGGTGTCATGTCTGGGGAAACTTCTGCTTGACCTACCAAGATCAAGAGCTTATTAATGACGGGTCGTTGTTGAGCAGTTTTGGTATCAAGGACAGCGACGAGGAG
GACATCCCGAGTTGAATAACTCATCATCCTGAGTTGAAGCATTTCTCCTTACAAATCCTCTTGTAAACAAATAGgtaaaagttttatgtggtatttttataggtttatatttttgtcaatcattgtcatgaacttgatccactgggttcatcagcacaaatcccaaatcttttttatttgataatttcttaattccttac
ttcctatatatgttgagaccactatacatgtgaatatcctattcaaacttgcaaggtcaatgtgatgataaatggctcgtggcttcatggcttctcaaagttgcatttctttcaatgaatgattgctcaatggattcctatgtttataaactttagtttttcatgaccgactctaatccagtactccttgctgggatgaa
cacagCTTTTTTTTGTACGGCATATGTCTTCAGCAAGTCAAGGGGAGAAACTTCATCGAAGAAAACATGGCACATTTGGAGTAATGAAAAAGAAGTCTTCTGATGGTGCAAATATGAATGGACGCAAAGCCTTTACCCAAATTGAGCTCCATTGGACACGCACCATAAGAACGTGGTTTGTCTATACAAAACTTTGGATT
ATTGGTATGAAAGCAGATTCCAAGAGAATACAACTAAGGCACTGAAACATGACAAGTGACAAATCAAATGTTCGAGAGATGTGTATATATATATATATATATATATATTTAAATCAGTAATTCACCTGCTTCCCTGAAATAATATATATTTATGAAGAATTGTTTGACTCAACTC