NA ----> NA

Intron 1 GTACTGTTCA ... TGTGTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.45	6	5	2
A2	31.25	11	5	1
A3	40	3	2	0
A4	28.57	5	2	0
A5	100	0	3	3
A6	26.09	17	6	3
A7	11.11	16	2	0
A8	41.67	7	5	1
A9	20	8	2	0
A10	0	10	0	0

>TRINITY_DN45039_c3_g1_i1 from sample A8 / Example spliced isoform

TATCCAATTGATTGAATTGTAAGGTTAGCTTTTATTCTGACGGTTATCTGAATCATTCTTCCTGTATCGTTGTTATGCTTAAGGCGATACATTTTCCTGACTGGCTGGTGCTTTGTTCTTGGATTTTGCTTTGCTACGACTAACCAGATCTGTATTATCGTTCTTCAAGAGGAGCTATGAATCCATCGTATGCTCGCCAC
TATCCATCAGGGCAAGAACAGGCGTTGCATTCTCCTCCTTCGACAGCTCAGAAGGAGGTAACTTCATGGCCTTCAATGAAGAGATCTCCTTCCACAGACTCGATCGGAGGAGCAAAGACCTGTGTTTGCTCTCCTACGACACATGCGGGTTCCTTCCGCTGCCGACTGCATAGAAATTCGGAGTCTTTTTCTTCAGCACA
CGGTCACCCGCCGCCTCCTGCTCCATCTCAGGATTCTTCTCCTCCTCTTCCTCCTTCCGCTGCGCCTCCATCAATGGCAAGTACGGCTTCCAGGACGGTCGAAGCGCAGTGAATCTTACTATAAAGCCTTTTTTTTCATCGAGTACCGCTTCTTGATACGATGCAAAGCCAGTCCGCAGCACAGCAAGGCTCATCAGTAG
TAATCTTTTTGTCAATTTTGTGTAAATATAGTATGGTCTTATCCACTATTTACATGAATTACTTGCAAAAGTTTCAGGTATTGCTATTCTTTACCCTATTTTGTGTGTACTAATGGTTGCCTGTAGGTTTCGAAGCAAATTGTTGTCTTGTACTAGACAGTGCGAAGATAATATAATTATCGAGATAGATATTATTTATA
TATATTCTTCTCTTAAGGGTTTATAAGCTCTGTTCTGGATTGAATTGCTTCTACTCGAAAGTACAATGTTTTCAGTTGAAATTCAGAGCCGTTTCTTCTCACCCATTAATAAATCTTTTTCTCAAGATGTTTAAGAGCTTCTTTTGCACAAAAACATTGCATCCTATTACAGTCACTGGATTCAACTTTTTCTGTACATT
CAATATGATGCAAGTCTTCATTAAGTCTTGATGCCCTCGATTTGTAAACAAATTGTAGAATTTTCTGATATATAATTTTTTCGAAATCAAATACTTGGC

>TRINITY_DN45039_c3_g1_i2 from sample A8 / Example unspliced isoform:

TATCCAATTGATTGAATTGTAAGGTTAGCTTTTATTCTGACGGTTATCTGAATCATTCTTCCTGTATCGTTGTTATGCTTAAGGCGATACATTTTCCTGACTGGCTGGTGCTTTGTTCTTGGATTTTGCTTTGCTACGACTAACCAGATCTGTATTgtactgttcatgtgtgcttgtgtgtatatatagttagatctgca
gatgcttgttcgtgttcttttcccgaaaccctagcggtgatgaatcgtgaaccctaatggtgtattttttgttgtgtatcagATCGTTCTTCAAGAGGAGCTA

Intron 2 GTTAGCTTTT ... TGTGTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	54.72	5	5	7.8
A2	31.63	17	8	8.5
A3	42.25	5	2.8	5
A4	23.57	16	5	5
A5	53.44	2	0	4.7
A6	13.65	68	7.2	14.7
A7	17.63	44	7.7	11.7
A8	8.54	45	1.7	6.8
A9	18.84	29	6.3	7.5
A10	18.94	17	0	8.3

>TRINITY_DN101769_c0_g1_i1 from sample A1 / Example spliced isoform

GGAAGCTTCTTGGCAATCTGATTCCTTTCCTTCGTTTTCTTCGCATTTGCTCTTTTCTCGTTGTGGTCGTGGCTTTTTCGTTTTTAATTTTCTGGCGGTTTCTGTTCGATCCGTTCAGTTGACCGTTCCGTTGGGGCTGGATTGCCTGATTATCCAATTGATTGAATTGTAAGATCGTTCTTCAAGAGGAGCTATGAATC
CATCGTATGCTCGCCACTATCCATCAGGGCAAGAACAGGCGTTGCATTCTCCTCCTTCGACAGCTCAGAAGGAGGTAACTTCATGGCCTTCAATGAAGAGATCTCCTTCCACAGACTCGATCGGAGGAGCAAAGACCTGTGTTTGCTCTCCTACGACACATGCGGGTTCCTTCCGCTGCCGACTGCATAGAAATTCGGAG
TCTTTTTCTTCAGCACACGGTCACCCGCCGCCTCCTGCTCCATCTCAGGATTCTTCTCCTCCTCTTCCTCCTTCCGCTGCGCCTCCATCAATG

>TRINITY_DN101769_c0_g1_i3 from sample A1 / Example unspliced isoform:

GGAAGCTTCTTGGCAATCTGATTCCTTTCCTTCGTTTTCTTCGCATTTGCTCTTTTCTCGTTGTGGTCGTGGCTTTTTCGTTTTTAATTTTCTGGCGGTTTCTGTTCGATCCGTTCAGTTGACCGTTCCGTTGGGGCTGGATTGCCTGATTATCCAATTGATTGAATTGTAAGgttagcttttattctgacggttatctg
aatcattcttcctgtatcgttgttatgcttaaggcgatacattttcctgactggctggtgctttgttcttggattttgctttgctacgactaaccagatctgtattgtactgttcatgtgtgcttgtgtgtatatatagttagatctgcagatgcttgttcgtgttcttttcccgaaaccctagcggtgatgaatcgtga
accctaatggtgtattttttgttgtgtatcagATCGTTCTTCAAGAGGAGCTATGAATCCATCGTATGCTCGCCACTATCCATCAGGGCAAGAACAGGCGTTGCATTCTCCTCCTTCGACAGCTCAGAAGGAGGTAACTTCATGGCCTTCAATGAAGAGATCTCCTTCCACAGACTCGATCGGAGGAGCAAAGACCTGTG
TTTGCTCTCCTACGACACATGCGGGTTCCTTCCGCTGCCGACTGCATAGAAATTCGGAGTCTTTTTCTTCAGCACACGGTCACCCGCCGCCTCCTGCTCCATCTCAGGATTCTTCTCCTCCTCTTCCTCCTTCCGCTGCGCCTCCATCAATG

Intron 3 GTATTGTACT ... TGTGTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60	6	5	9
A2	47.62	11	4	10
A3	66.67	3	2	6
A4	60	4	2	6
A5	100	0	2	5
A6	53.13	15	7	17
A7	46.67	16	2	14
A8	57.14	6	5	8
A9	52.94	8	2	9
A10	55.56	8	0	10

>TRINITY_DN33888_c0_g1_i2 from sample A6 / Example spliced isoform

GGGACGGAGGATCAAGTGGCTTCTGCCGGCCGTTAAGGCGGGCAGTAGGTCCCTCCGTTCGGTCAGGATGCCAACGCCTCCTCCACCGCCTCACCACCGAAACCCTCCGCTCCCTCTACCCAAACTCGCCGCTCCATCCTCATTCCCATCTATAAATAGCAGAGCCTACCAGTGTAATGCAGTTTGCCGCTCTGTCTCTG
GAAGCTTCTTGGCAATCTGATTCCTTTCCTTCGTTTTCTTCGCATTTGCTCTTTTCTCGTTGTGGTCGTGGCTTTTTCGTTTTTAATTTTCTGGCGGTTTTTGTTCGATCCGTTCAGTTGACCGTTCCGTTGGGGCTGGATTGCCTGATTATCCAATTGATTGAATTGTAAGGTTAGCTTTTATTCTGACGGTTATCTGA
ATCATTCTTCCTGTATCGTTGTTATGCTTAAGGCGATACATTTTCCTGACTGGCTGGTGCTTTGTTCTTGGATTTTGCTTTGCTACGACTAACCAGATCTATCGTTCTTCAAGAGGAGCTATGAATCCATCGTATGCTCGCCACTATCCATCAGGGCAAGAACAGGCGTTGCATTCTCCTCCTTCGACAGCTCAGAAGGA
GGTAACTTCATGGCCTTCAATGAAGAGATCTCCTTCCACAGACTCGATCGGAGGAGCAAAGACCTGTGTTTGCTCTCCTACGACACATGCGGGTTCCTTCCGCTGCCGACTGCATAGAAATTCGGAGTCTTTTTCTTCAGCACACGGTCACCCGCCGCCTCCTGCTCCATCTCAGGATTCTTCTCCTCCTCTTCCTCCTT
CCGCTGCGCCTCCATCAATGGCAAGTACGGCTTCCAGGACGGTCGAAGCGCAGTGAATCTTACTATAAAGCCTTTTTTTTCATCGAGTACCGCTTCTTGATACGATGCAAAGCCAGTCCGCAGCACAGCAAGGCTCATCAGTAGTAATCTTTTTGTCAATTTTGTGTAAATATAGTATGGTCTTATCCACTATTTACATG
AATTACTTGCAAAAGTTTCAGGTATTGCTATTCTTTACCCTATTTTGTGTGTACTAATGGTTGCCTGTAGGTTTCGAAGCAAATTGTTGTCTTGTACTAGACAGTGCGAAGATAATATAATTATCGAGATAGATATTATTTATATATATTCTTCTCTTAAGGGTTTATAAGCTCTGTTCTGGATTGAATTGCTTCTACTC
GAAAGTACAATGTTTTCAGTTGAAATTCAGAGCCGTTTCTTCTCACCCATTAATAAATCTTTTTCTCAAGATGTTTAAGAGCTTCTTTTGCACAAAAAAATTGCATCCTATTACAGTCACTGGATTCAACTTTTTCTGTACATTCAATATGATGCAAGTCTTCATTAAGTCTTGAT

>TRINITY_DN33888_c0_g1_i1 from sample A6 / Example unspliced isoform:

GGGACGGAGGATCAAGTGGCTTCTGCCGGCCGTTAAGGCGGGCAGTAGGTCCCTCCGTTCGGTCAGGATGCCAACGCCTCCTCCACCGCCTCACCACCGAAACCCTCCGCTCCCTCTACCCAAACTCGCCGCTCCATCCTCATTCCCATCTATAAATAGCAGAGCCTACCAGTGTAATGCAGTTTGCCGCTCTGTCTCTG
GAAGCTTCTTGGCAATCTGATTCCTTTCCTTCGTTTTCTTCGCATTTGCTCTTTTCTCGTTGTGGTCGTGGCTTTTTCGTTTTTAATTTTCTGGCGGTTTTTGTTCGATCCGTTCAGTTGACCGTTCCGTTGGGGCTGGATTGCCTGATTATCCAATTGATTGAATTGTAAGGTTAGCTTTTATTCTGACGGTTATCTGA
ATCATTCTTCCTGTATCGTTGTTATGCTTAAGGCGATACATTTTCCTGACTGGCTGGTGCTTTGTTCTTGGATTTTGCTTTGCTACGACTAACCAGATCTgtattgtactgttcatgtgtgcttgtgtgtatatatagttagatctgcagatgcttgttcgtgttcttttcccgaaaccctagcggtgatgaatcgtgaa
ccctaatggtgtattttttgttgtgtatcagATCGTTCTTCAAGAGGAGCTATGAATCCATCGTATGCTCGCCACTATCCATCAGGGCAAGAACAGGCGTTGCATTCTCCTCCTTCGACAGCTCAGAAGGAGGTAACTTCATGGCCTTCAATGAAGAGATCTCCTTCCACAGACTCGATCGGAGGAGCAAAGACCTGTGT
TTGCTCTCCTACGACACATGCGGGTTCCTTCCGCTGCCGACTGCATAGAAATTCGGAGTCTTTTTCTTCAGCACACGGTCACCCGCCGCCTCCTGCTCCATCTCAGGATTCTTCTCCTCCTCTTCCTCCTTCCGCTGCGCCTCCATCAATGGCAAGTACGGCTTCCAGGACGGTCGAAGCGCAGTGAATCTTACTATAAA
GCCTTTTTTTTCATCGAGTACCGCTTCTTGATACGATGCAAAGCCAGTCCGCAGCACAGCAAGGCTCATCAGTAGTAATCTTTTTGTCAATTTTGTGTAAATATAGTATGGTCTTATCCACTATTTACATGAATTACTTGCAAAAGTTTCAGGTATTGCTATTCTTTACCCTATTTTGTGTGTACTAATGGTTGCCTGTA
GGTTTCGAAGCAAATTGTTGTCTTGTACTAGACAGTGCGAAGATAATATAATTATCGAGATAGATATTATTTATATATATTCTTCTCTTAAGGGTTTATAAGCTCTGTTCTGGATTGAATTGCTTCTACTCGAAAGTACAATGTTTTCAGTTGAAATTCAGAGCCGTTTCTTCTCACCCATTAATAAATCTTTTTCTCAA
GATGTTTAAGAGCTTCTTTTGCACAAAAAAATTGCATCCTATTACAGTCACTGGATTCAACTTTTTCTGTACATTCAATATGATGCAAGTCTTCATTAAGTCTTGAT