sp|O48700|PUB6_ARATH ----> U-box domain-containing protein 6

Intron 1 GTTTCGGATA ... GTTTTCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.95	28.7	24.8	39.6
A2	60.35	21	29.4	33.9
A3	52.03	30.2	32.8	31.8
A4	37.7	16.2	4	15
A5	61.08	5.3	8	8.6
A6	61.52	48.8	70.3	84.6
A7	50.29	114.1	108.5	117.3
A8	49.22	66.6	55.7	69.8
A9	61.42	80.7	121	133.5
A10	59.39	71.4	98	107.5

>TRINITY_DN126166_c4_g1_i9 from sample A1 / Example spliced isoform

TTCATATGTAACTCCATTCGATGCCTTACAGCGCATAGTTCTGATCAATGAGCACTTGACATGGATGCGGCATTCTATCCGCTGGAAGGTTTGAACTATCAAGGATGAAAATCCGACCAGATCATGCATGCGGGAATTGGAAGACCAATGCGCAGGCAGAGCTGCACAGAAAATTTCAGAGTCTTTTGGGGATTCCAGGC
AGCAGAAATTCATGCAACCATAGCAATGGAGCAAGTCAATTTAAGATCTTCAAATGTTTTCTCACAAGACTTTACACATACCACTTCCAGGAAGACATCAACCTTCAACTGCTTTGTCAAATAGCCCCAAAACTCCCAGGAGACTCAGTTTTGATCAGATTTGAGAAGGCCAAGCATTCTTTGGATCATTGTTTGAGACG
TGTTGAATGCATAGTGCCACAAATATTATGGGAGCAGTCTTTACAAGTTGTAGGTACCTGATGGAGTTGCTGATGGTTGAAAGTCGAAAATTTTAGCAAAAAGTGTTCCCAGAGAATAACATATATACGAACAGGCATCCACATATATACGAACAGAAAATTGTTTGAATGAATCGAGAGAGTGTCGGCCAATCTTTAAT
CCCGTGAATTGTCGCAGATGGAAGACACGTCACCCCATGTGAATAGCAGATTCTTATCCTTTATTGGTAGGGTTCGTTGTTCAGCGGCCCTATATGTTTGTCTTATCTTTGCCACGTGGTAAAGGAATATATGGTAGGTCATGGGTGGTGTAACTTCATCCGTATGGCAGGGTGCAGTGGAGTGGGGCTAAGACTATAAC
AATCTCACGGTCGAGTTTCTTAATGTTTTTTAAAATTAAATACTCCGTAG

>TRINITY_DN126166_c4_g1_i2 from sample A1 / Example unspliced isoform:

TTCATATGTAACTCCATTCGATGCCTTACAGCGCATAGTTCTGATCAATGAGCACTTGACATGGATGCGGCATTCTATCCGCTGGAAGGTTTGAACTATCAAGGATGAAAATCCGACCAGATCATGCATGCGGGAATTGGAAGACCAATGCGCAGGCAGAGCTGCACAGAAAATTTCAGAGTCTTTTGGGGATTCCAGGC
AGCAGAAATTCATGCAACCATAGCAATGGAGCAAGTCAATTTAAGgtttcggatataaaatggatgaatggattaataacagatcttcaaatgttttctcagATCTTCAAATGTTTTCTCACAAGACTTTACACATACCACTTCCAGGAAGACATCAACCTTCAACTGCTTTGTCAAATAGCCCCAAAACTCCCAGGAGA
CTCAGTTTTGATCAGATTTGAGAAGGCCAAGCATTCTTTGGATCATTGTTTGAGACGTGTTGAATGCATAGTGCCACAAATATTATGGGAGCAGGTGGGTAGCTGAGAAATCACTATCTTTTTTGGAGTTTTCGTTCTTAACTTTATACATTCATCTAGGAAATTTGTCAATGTGCATTGAATATTCAATACAGTGGAGA
AGTGATATAATTTTTTTCTTTTTTATATATGCGTACGAGGACAGGGGCATCTACTATAT

Intron 2 GTATTTTTTT ... CTCTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.68	26.6	2.4	7.9
A2	15.46	26.8	4.8	5
A3	16.56	53	12.7	10.5
A4	32.4	9.7	1	3.9
A5	23.83	12.3	3.4	3.8
A6	23.49	45.3	6.3	13.9
A7	24.39	117.9	13	38.1
A8	21.07	71.6	15.1	18.9
A9	22.1	123.4	15.4	35.7
A10	20.04	81.8	13.6	20.7

>TRINITY_DN107397_c9_g1_i1 from sample A3 / Example spliced isoform

CTTCTTTAATTTGTGGTTGTCATATATGTTGAGATCCCATTCACATTAATGTTAATCTCCTTTCTCCTTAAAAGCTCACGAGATCTACATTTGATTTTTCTCCCTCCTGTTTGTCTTCATATGTAACTCCATTCGATGCCTTACAGCGCATAGTTCTGATCAATGAGCACTTGACATGGATGCGGCATTCTATCCGCTGG
AAGGTTTGAACTATCAAGGATGAAAATCCGACCAGATCATGCATGCGGGAATTGGAAGACCAATGCGCAGGCAGAGCTGCACAGAAAATTTCAGAGTCTTTTGGGGATTCCAGGCAGCAGAAATTCATGCAACCATAGCAATGGAGCAAGTCAATTTAAGATCTTCAAATGTTTTCTCACAAGACTTTACACATACCACT
TCCAGGAAGACATCAACCTTCAACTGCTTTGTCAAATAGCCCCAAAACTCCCAGGAGACTCAGTTTTGATCAGATTTGAGAAGGCCAAGCATTCTTTGGATCATTGTTTGAGACGTGTTGAATGCATAGTGCCACAAATATTATGGGAGCAGTCTTTACAAGTTGTAGGTACCTGATGGAGTTGCTGATGGTTGAAAGTC
GAAAATTTTAGCAAAAAGTGTTCCCAGAGAATAACATATATACGAACAGGCATCCACATATATACGAACAGAAAATTGTTTGAATGAATCGAGAGAGTGTCGGCCAATCTTTAATCCCGTGAATTGTCGCAGATGGAAGACACGTCACCCCATGTGAATAGCAGATTCTTATCCTTTATTGGTAGGGTTCGTTGTTCAGC
GGCCCTATATGTTTGTCTTATCTTTGCCACGTGGTAAAGGAATATATGGTAGGTCATGGGTGGTGTAACTTCATCCGTATGGCAGGGTGCAGTGGAGTGGGGCTAAGACTATAACAATCTCACGGTCGAGTTTCTTAATGTTTTTTAAAATTAAATACTCCGTAGCTTTTCGTTTTAAAAATGTCATCCAAAAACCACTC
TTAAACAATTTTGCCAATTATGAAAGATGGTAAATATTATAAAATTCAAAAAATCATATTTTGAAGCATTCAATAAAAT

>TRINITY_DN107397_c9_g1_i9 from sample A3 / Example unspliced isoform:

CTTCTTTAATTTGTGGTTGTCATATATGTTGAGATCCCATTCACATTAATGTTAATCTCCTTTCTCCTTAAAAGCTCACGAGATCTACATTTGATTTTTCTCCCTCCTGTTTGTCTTCATATGTAACTCCATTCGATGCCTTACAGCGCATAGTTCTGATCAATGAGCACTTGACATGGATGCGGCATTCTATCCGCTGG
AAGGTTTGAACTATCAAGGATGAAAATCCGACCAGATCATGCATGCGGGAATTGGAAGACCAATGCGCAGGCAGAGCTGCACAGAAAATTTCAGAGTCTTTTGGGgtattttttttagggttgcattcagcgcaatttgacagtgattcttcaatcttccatagccattgtctagggaaatatttatgctaggtgcatgg
tgaaggatatatacattatatttaacctccgaaggaattttttcaagtttgcaaaatgtatcaatgctctgtacagGATTCCAGGCAGCAGAAATTCATGCAACCATAGCAATGGAGCAAGTCAATTTAAGGTTTCGGATATAAAATGGATGAATGGATTAATAACAGATCTTCAAATGTTTTCTCAGATCTTCAAATGT
TTTCTCACAAGACTTTACACATACCACTTCCAGGAAGACATCAACCTTCAACTGCTTTGTCAAATAGCCCCAAAACTCCCAGGAGACTCAGTTTTGATCAGATTTGAGAAGGCCAAGCATTCTTTGGATCATTGTTTGAGACGTGTTGAATGCATAGTGCCACAAATATTATGGGAGCAGGTGGGTAGCTGAGAAATCAC
TATCTTTTTTGGAGTTTTCGTTCTTAACTTTATACATTCATCTAGGAAATTTGTCAATGTGCATTGAATATTCAATACAGTGGAGAAGTGATATAATTTTTTTCTTTTTTATATATGCGTACGAGGACATGGGCATCTACTATATTTAAATAAAAAATGTACATTTTAGCTTTGTTCCACTTGAAACCATCCAACATAAT
TGAGCCAAGGGGATCTAAGTTTAGTTGAATGGATTGTTCTG