NA ----> NA

Intron 1 GCAAGCGAGA ... TGTACCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.92	3	12	8
A2	100	0	3	6
A3	52.38	5	5	6
A4	33.33	1	1	0
A5	100	0	2	1
A6	76.47	14	30	61
A7	72.63	13	25	44
A8	73.98	16	23	68
A9	64.89	33	51	71
A10	75	7	16	26

>TRINITY_DN38966_c0_g1_i6 from sample A9 / Example spliced isoform

CTCCAAATGTTTTGTTCACTTCAAATGCATTGTTTATGTACTTGCCTGAATTATTAGAAAGCTGGAAAACGATTTATAGTTTCAAGTTTTGGGTTTAATGGCACCAGGATGGTCAGATGGAAGCAAGTATGGAGGAAAGTTTCGAATTATGTGCGTTATGATCTGAAAGAGATTGCATTTCCATCCTCTCTGCCAGACCC
CCCTCATTTAAAACCGCGGAAGAAGCTAACATGGCCCGAGTGGTTAGAGGTATTAAAGAAAGCTTCCAAACTGTATGTAGAAAGCTGGAAGCGCGACATTAGTGATGAGATTGATGTGCTGGAAGGCAGAGCCCCACCACCCAAAACAGGAAATGGTTCAACGACAGAAAATGGAAGGCGTGACAGGGAACCCTCAACAC
TGGAGGATGTAGCTGTGGCAGCAAGAGGCGGCATGGAAACCTTACGTCCTGTACTACAGCGAATCTATATGACTAGAGCGACAGCTTATAAAGATGCCCTTAAAAACTTTATTCAAGGTTATCAAGAAGGTATATCAGAAACAATGGCTTCAAAAGAATTTCTGAAAACAGCCGTACATGAAGAAGATAGGAATTCAACA
AAAGAAGAAAAGAAAGAAGCTTTAGTATGGATCTCGACACACAAGCAGTGTTGCAACCCCCATTAAACAAGTTTTCTAACATGCTGCATATTTCTGTTAGTTGGCATGAACCCTTTTAATTACAAAACTTAGAGATCTGTTAACTGAAATCTATTCATGCTTTATGTCTTAAATATAGGTATAATCCCTTTAGGCAAAGT
AATTTCAGATGTTTTGTATTTACTATTAATTATTGTAGAAAGACAGCTTTTATTCCCTCCATCAAAGAACCAAATTGCCATTCAGAACATAAAATATGAAATATGAAAGCATGACCTTTCTACAAAAGGTAAAATGAATGTATGGGAAAGGGATAACTTATGCAACTTTGTTACAACCTGATTTTTTTCATTGGCTAGAG
CAGAAATTGCTTTATCAAAAATTTGTTCTTTAATAAGTTAGTTTTGATGAAGAGGAATTTCAAGAAGTTGCTTGCCTCACTGAAGTGGCTGAAAATATGCACGATGCTTTCAATTTTATTATCAATCTGGTTTACTCATTTTGGGACTTTGAAGCCAATGTCTTTTTACTGATGGTAATGCCAAACAAATTTTTTTGAAG
CCGTGAATTGCTTTAATTTATAAAAAATTCCCG

>TRINITY_DN38966_c0_g1_i2 from sample A9 / Example unspliced isoform:

ATTGGAGGACTTGTGCAATGATGAAACTCTCTGTACAATTCTGTTTTCACAGGTATTAAAGAAAGCTTCCAAACTGTATGTAGAAAGCTGGAAGCGCGACATTAGTGATGAGATTGATGTGCTGGAAGGCAGAGCCCCACCACCCAAAACAGGAAATGGTTCAACGACAGAAAATGGAAGGCGTGACAGGGAACCCTCAA
CACTGGAGGATGTAGCTGTGGCAGCAAGAGGCGGCATGGAAACCTTACGTCCTGTACTACAGCGAATCTATATGACTAGAGCGACAGCTTATAAAGATGCCCTTAAAAACTTTATTCAAGGTTATCAAGAAGGTATATCAGAAACAATGGCTTCAAAAGAATTTCTGAAAACAGCCGTACATGAAGAAGATAGGAATTCA
ACAAAAGAAGAAAAGAAAGAAGgcaagcgagagcaatgaaacttaagttttgtatgttgcatcaaatggtttgctttatttattgagattttagacggagtggaattgaaaaatttcctcccttgaaagattaatttttcaattgttcttgtacctcagCTTTAGTATGGATCTCGACACACAAGCAGTGTTGCAACCCC
CATTAAACAAGTTTTCTAACATGCTGCATATTTCTGTTAGTTGGCATGAACCCTTTTAATTACAAAACTTAGAGATCTGTTAACTGAAATCTATTCATGCTTTATGTCTTAAATATAGGTATAATCCCTTTAGGCAAAGTAATTTCAGATGTTTTGTATTTACTATTAATTATTGTAGAAAGACAGCTTTTATTCCCTCC
ATCAAAGAACCAAATTGCCATTCAGAACATAAAATATGAAATATGAAAGCATGACCTTTCTACAAAAGGTAAAATGAATGTATGGGAAAGGGATAACTTATGCAACTTTGTTACAACCTGATTTTTTTCATTGGCTAGAGCAGAAATTGCTTTATCAAAAATTTGTTCTTTAATAAGTTAGTTTTGATGAAGAGGAATTT
CAAGAAGTTGCTTGCCTCACTGAAGTGGCTGAAAATATGCACGATGCTTTCAATTTTATTATCAATCTGGTTTACTCATTTTGGGACTTTGAAGCCAATGTCTTTTTACTGATGGTAATGCCAAACAAATTTTTTTGAAGCCGTGAATTGCTTTAATTTATAAAAAATTCCCG

Intron 2 GTATTAAAGA ... CTGTATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.9	8	12	14
A2	79.17	5	19	19
A3	73.68	5	15	13
A4	47.37	10	12	6
A5	64.29	5	8	10
A6	64.89	33	65	57
A7	63.17	58	106	93
A8	66	34	72	60
A9	63.57	53	95	90
A10	69.11	38	88	82

>TRINITY_DN39419_c0_g1_i2 from sample A7 / Example spliced isoform

CTAAAATAAAAGACAAAATTTGAACACAAACCACGAAGCAAGAGTAATGCCTCGTAACAATTCACCTTGATCCCATTCCTGCTATATGGGATATAAACATTTTGGTTGGAATCGGCATTGAGTTTGTGACTATGGATTACTGAAGTCGAGTGTTGAGTAAAGCAAGATTATAAATACAGCCTCGACTTGAAAGCCCATTG
ACAATAGCTCTCTTATGTTTGCAGGATGGTCAGATGGAAGCAAGTATGGAGGAAAGTTTCGAATTATGTGCGTTATGATCTGAAAGAGATTGCATTTCCATCCTCTCTGCCAGACCCCCCTCATTTAAAACCGCGGAAGAAGCTAACATGGCCCGAGTGGTTAGAGAAAGCTGGAAGCGCGACATTAGTGATGAGATTGA
TGTGCTGGAAGGCAGAGCCCCACCACCCAAAACAGGAAATGGTTCAACGACAGAAAATGGAGGGCGTGACAGGGAACCCTCAACACTGGAGGATGTAGCTGTGGCAGCAAGAGGCGGCATGGAAACCTTACGTCCTGTACTACAGCGAATCTATATGACTAGAGCGACAGCTTATAAAGATGCCCTTAAAAACTTTATTC
AAGGTTATCAAGAAGGTATATCAGAAACAATGGCTTCAAAAGAATTTCTGAAAACAGCCGTACATGAAGAAGATAGGAATTCAACAAAAGAAGAAAAGAAAGAAGGCAAGCGAGAGCAATGAAACTTAAGTTTTGTATGTTGCATCAAATGGTTTGCTTTATTTATTGAGATTTTAGACGGAGTGGAATTGAAAAATTTC
CTCCCTTGAAAGATTAATTTTTCAATTGTTCTTGTACCTCAGCTTTAGTATGGATCTCGACACACAAGCAGTGTTGCAACCCCCATTAAACAAGTTTTCTAACATGCTGCATATTTCTGTTAGTTGGCATGAACCCTTTTAATTACAAAACTTAGAGATCTGTTAACTGAAATCTATTCATGCTTTATGTCTTAAATATA
GGTATAATCCCTTTAGGCAAAGTAATTTCAGATGTTTTGTATTTACTATTAATTATTGTAGAAAGACAGCTTTTATTCCCTCCATCAAAGAACCAAATTGCCATTCAGAACATAAAATATGAAATATGAAAGCATGAGCTTTCTACAAAAGGTAAAATGAATGTATGGGAAAGGGATAACTTATGCAACTTTGTTACAAC
CTGATTTTTTTCATTGGCTAGAGCAGAAATTGCTTTATCAAAAATTTGTTCTTTAATAAGTTAGTTTTGATGAAGAGGAATTTCAAGAAGTTGCTTGCCTCACTGAAGTGGCTGAAAATATGCACGATGCTTTCAATTTTATTATCAATCTGGTTTACTCATTTTGGGACTTTGAAGCCAATGTCTTTTAACTGATGGTA
ATGCCAAACAAAATTTTTTGAAGCCGTGAATTGCTTTAATTTGTAAAAAATTCCCTGG

>TRINITY_DN39419_c0_g1_i1 from sample A7 / Example unspliced isoform:

CTAAAATAAAAGACAAAATTTGAACACAAACCACGAAGCAAGAGTAATGCCTCGTAACAATTCACCTTGATCCCATTCCTGCTATATGGGATATAAACATTTTGGTTGGAATCGGCATTGAGTTTGTGACTATGGATTACTGAAGTCGAGTGTTGAGTAAAGCAAGATTATAAATACAGCCTCGACTTGAAAGCCCATTG
ACAATAGCTCTCTTATGTTTGCAGGATGGTCAGATGGAAGCAAGTATGGAGGAAAGTTTCGAATTATGTGCGTTATGATCTGAAAGAGATTGCATTTCCATCCTCTCTGCCAGACCCCCCTCATTTAAAACCGCGGAAGAAGCTAACATGGCCCGAGTGGTTAGAGgtattaaagaaagcttccaaactgtatgtagAAA
GCTGGAAGCGCGACATTAGTGATGAGATTGATGTGCTGGAAGGCAGAGCCCCACCACCCAAAACAGGAAATGGTTCAACGACAGAAAATGGAGGGCGTGACAGGGAACCCTCAACACTGGAGGATGTAGCTGTGGCAGCAAGAGGCGGCATGGAAACCTTACGTCCTGTACTACAGCGAATCTATATGACTAGAGCGACA
GCTTATAAAGATGCCCTTAAAAACTTTATTCAAGGTTATCAAGAAGGTATATCAGAAACAATGGCTTCAAAAGAATTTCTGAAAACAGCCGTACATGAAGAAGATAGGAATTCAACAAAAGAAGAAAAGAAAGAAGGCAAGCGAGAGCAATGAAACTTAAGTTTTGTATGTTGCATCAAATGGTTTGCTTTATTTATTGA
GATTTTAGACGGAGTGGAATTGAAAAATTTCCTCCCTTGAAAGATTAATTTTTCAATTGTTCTTGTACCTCAGCTTTAGTATGGATCTCGACACACAAGCAGTGTTGCAACCCCCATTAAACAAGTTTTCTAACATGCTGCATATTTCTGTTAGTTGGCATGAACCCTTTTAATTACAAAACTTAGAGATCTGTTAACTG
AAATCTATTCATGCTTTATGTCTTAAATATAGGTATAATCCCTTTAGGCAAAGTAATTTCAGATGTTTTGTATTTACTATTAATTATTGTAGAAAGACAGCTTTTATTCCCTCCATCAAAGAACCAAATTGCCATTCAGAACATAAAATATGAAATATGAAAGCATGAGCTTTCTACAAAAGGTAAAATGAATGTATGGG
AAAGGGATAACTTATGCAACTTTGTTACAACCTGATTTTTTTCATTGGCTAGAGCAGAAATTGCTTTATCAAAAATTTGTTCTTTAATAAGTTAGTTTTGATGAAGAGGAATTTCAAGAAGTTGCTTGCCTCACTGAAGTGGCTGAAAATATGCACGATGCTTTCAATTTTATTATCAATCTGGTTTACTCATTTTGGGA
CTTTGAAGCCAATGTCTTTTAACTGATGGTAATGCCAAACAAAATTTTTTGAAGCCGTGAATTGCTTTAATTTGTAAAAAATTCCCTGG