sp|P92939|ECA1_ARATH ----> Calcium-transporting ATPase 1, endoplasmic reticulum-type

Intron 1 GTAAGAATTT ... TTTTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	98.5	2	144	12
A2	100	0	152	16
A3	97.16	6	216.5	13
A4	93.09	9	129	11
A5	98.33	2	124.5	3
A6	77.7	19	67.5	7
A7	89.2	16	134	16
A8	77.97	8	29	5
A9	90.63	10	99	17
A10	91.42	13	140.5	22

>TRINITY_DN45175_c0_g2_i2 from sample A6 / Example spliced isoform

ATCGCGTGGTTGATGTGATAAATTCTGATCTGGTGTGGTTCTTCCTGGCGTGTCTTTCACAAAGTTTTCTTTACACGCTGTCTTGCGCTACTTCCTATCGTATTCATGGCATGTTCAGAGAGTTTCCTGCGCGGTACATATGGAAGGGAACCACCCACCGACTCTGTCTTCCTCGATATCCATGACATCACCAAACTCGA
TGTGCTCTTCCTTAGGCTCGAGCCTACAAGTTTTGTCTATGGCAGCGAGTTGGTGAAAGCGCTCCAAATGCATTTTCAAAGAGAAAGGGTTTCTACATAACGACCTCGGACCCAAAAACTTTTCCCGCCTAGGCTAGAGATGAAGAGGATTGCATTTAAGAATTTGAAGTAGATATTAGACGAGGGCTGAGTAACTTTTC
CCGCCTAGGCTAGAGATGAAGAGGGTTGCATTCACGAATTTGAAGTAGATATTAGACGAGGGCTTAGTAGCGAGGATGTCGAGAGACGGTGGAAAAAATGGGGCTGGAACAAGCTGGAAAAGCATCCCAAGCCGTTAGTTTGGAAGCTCATCTTGGACCAATTTAGTGACACGCTGGGGGTGGCCCAAGCATGTGATCAT
TTTTCATCATATATGCCATCCAATATGGGAAGAGAGGGGCGCTCAAATAATCCTCCAATAAGCTGCTGTTGAAAGCAACTCCAAATACTGATCATGAGTCAAATCTTCCCAAGGAATGAAGCATGCACCTAGTGTTTCAGTGATGTTCCAAAATTTCAGTCTAGAAGAAGTGCTTCTATGTATCCCAGAACAACAAACTA
TGAGGGGCGTTTCCCTAAAGAACCCATTTAACTCTACTAGTTCAGAACAGTGCCCTGGAAAATGCAACATTGGGGTATTATCTTCGGTTTCATATGCTGTCATGCACCTTCAACCTGCTATCATAATGCTACAGGACCCATTTCACTGAGATTATGCCTTCATTCATGGGCTTTGACTGGCTGGAAACTTCCCCTTTTTC
CTGAGTGTTGATCGGCATACGATTCATCCCATGGTAGATATTGATTTAGTTTTTCATCCTATAGTTCACTACAGAAGACAGAGCAGGCAGTGCAGACAAGCTTAGAAGGCATCTTAGGTAGGATTCTGGATGTAGGGTAACAGTTTCACGAAGTTGTTTCTCATGTTCCTAAATATTTGAAGGTAATCATAGACATCGGA
AAAGAGATTTTCAATGCTTGGCTTTTCCATGCGCTCAACAAATTGTATTGCCCCTAAAATTTATACAGACCAAAAGTAAAGTTAATTTCTTGAACATGGTTCAAGTGGATTATTGATGGCTTAAAGTTGTGAATACCATAAAACACTATTCTATACAAAATGAGGCTTCGCTGCTCAAGTATACCTTGTTTTGTATTTCA
GTCGGGAGGTTCGATTCAGCCTTCTCAGTCCACCAATTGTTTTGATTCATCCCATTTCTATACCTTGACACAGGGTCATGTTTCAACTTCCAATATTCATTTTGTTGAATCATCGGAGCACATTTTATCTGCAATTACTATCAGGACAAACATCAAGGAAGAAAGAAAATAAATATGTTGACACTTTCTTGGAATTATTG
AGCTCAGATTCTAGAGATAGTCAACAAATTGGTGGTTAGCATTTTTGTTGAGTGTTTTGTTATGACTTTTGTTGTTGCATTTCAATATAGAGTTTCC

>TRINITY_DN45175_c0_g2_i4 from sample A6 / Example unspliced isoform:

ATCGCGTGGTTGATGTGATAAATTCTGATCTGGTGTGGTTCTTCCTGGCGTGTCTTTCACAAAGTTTTCTTTACACGCTGTCTTGCGCTACTTCCTATCGTATTCATGGCATGTTCAGAGAGTTTCCTGCGCGGTACATATGGAAGGGAACCACCCACCGACTCTGTCTTCCTCGATATCCATGACATCACCAAACTCGA
TGTGCTCTTCCTTAGGCTCGAGCCTACAAGTTTTGTCTATGGCAGCGAGTTGGTGAAAGCGCTCCAAATGCATTTTCAAAGAGAAAGGGTTTCTACATAACGACCTCGGACCCAAAAACTTTTCCCGCCTAGGCTAGAGATGAAGAGGATTGCATTTAAGAATTTGAAGTAGATATTAGACGAGGGCTGAGTAACTTTTC
CCGCCTAGGCTAGAGATGAAGAGGGTTGCATTCACGAATTTGAAGTAGATATTAGACGAGGGCTTAGTAGCGAGGATGTCGAGAGACGGTGGAAAAAATGGGGCTGGAACAAGCTGGAAAAGCATCCCAAGCCGTTAGTTTGGAAGCTCATCTTGGACCAATTTAGTGACACGCTGgtaagaatttttatagtgcccact
tttgtttcatttgtttttgcttggattgatggcgatgaaggcggtgaaatggatatcactgctttcgtcgagcagctatttattttcttgactttgattgtcaatgctaccatcggtgtttagcaagagagtaatgcggagaaagccttggaggctttgaaggaaatgcagtcggaacatgccatggtgagaagggatga
taatttcattacgaatttttctgcagGGGGTGGCCCAAGCATGTGATCATTTTTCATCATATATGCCATCCAATATGGGAAGAGAGGGGCGCTCAAATAATCCTCCAATAAGCTGCTGTTGAAAGCAACTCCAAATACTGATCATGAGTCAAATCTTCCCAAGGAATGAAGCATGCACCTAGTGTTTCAGTGATGTTCCA
AAATTTCAGTCTAGAAGAAGTGCTTCTATGTATCCCAGAACAACAAACTATGAGGGGCGTTTCCCTAAAGAACCCATTTAACTCTACTAGTTCAGAACAGTGCCCTGGAAAATGCAACATTGGGGTATTATCTTCGGTTTCATATGCTGTCATGCACCTTCAACCTGCTATCATAATGCTACAGGACCCATTTCACTGAG
ATTATGCCTTCATTCATGGGCTTTGACTGGCTGGAAACTTCCCCTTTTTCCTGAGTGTTGATCGGCATACGATTCATCCCATGGTAGATATTGATTTAGTTTTTCATCCTATAGTTCACTACAGAAGACAGAGCAGGCAGTGCAGACAAGCTTAGAAGGCATCTTAGGTAGGATTCTGGATGTAGGGTAACAGTTTCACG
AAGTTGTTTCTCATGTTCCTAAATATTTGAAGGTAATCATAGACATCGGAAAAGAGATTTTCAATGCTTGGCTTTTCCATGCGCTCAACAAATTGTATTGCCCCTAAAATTTATACAGACCAAAAGTAAAGTTAATTTCTTGAACATGGTTCAAGTGGATTATTGATGGCTTAAAGTTGTGAATACCATAAAACACTATT
CTATACAAAATGAGGCTTCGCTGCTCAAGTATACCTTGTTTTGTATTTCAGTCGGGAGGTTCGATTCAGCCTTCTCAGTCCACCAATTGTTTTGATTCATCCCATTTCTATACCTTGACACAGGGTCATGTTTCAACTTCCAATATTCATTTTGTTGAATCATCGGAGCACATTTTATCTGCAATTACTATCAGGACAAA
CATCAAGGAAGAAAGAAAATAAATATGTTGACACTTTCTTGGAATTATTGAGCTCAGATTCTAGAGATAGTCAACAAATTGGTGGTTAGCATTTTTGTTGAGTGTTTTGTTATGACTTTTGTTGTTGCATTTCAATATAGAGTTTCC

Intron 2 GTAAGAATTT ... CGGTGTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	99.54	1	188	244
A2	99.19	2	184	305
A3	98.2	6	267	388
A4	99.54	1	162	275
A5	99.42	1	154	191
A6	94.31	7	78	154
A7	97.82	5	151	297
A8	96.04	2	34	63
A9	94.67	10	115	240
A10	95.46	11	158	305

>TRINITY_DN48361_c1_g2_i2 from sample A10 / Example spliced isoform

AACTTTTCCCGCCTAGGCTAGAGATGAAGAGGGTTGCATTCACGAATTTGAAGTAGATATTAGACGAGGGCTGAGTAACTTTTCCCGCCTAGGCTAGAGATGAAGAGGGTTGCATTCACGAATTTGAAGTAGATATTAGACGAGGGCTTAGTAGCGAGGATGTCGAGAGACGGTGGAAAAAATGGGGCTGGAACAAGCTG
GAAAAGCATCCCAAGCCGTTAGTTTGGAAGCTCATCTTGGACCAATTTAGTGACACGCTGCAAGAGAGTAATGCGGAGAAAGCCTTGGAGGCTTTGAAGGAAATGCAGTCGGAACATGCCATGGTGAGAAGGGATGATAATTTCATTACGAATTTTTCTGCAGGGGGTGGCCCAAGCATGTGATCATTTTTCATCATATA
TGCCATCCAATATGGGAAGAGAGGGGCGCTCAAATAATCCTCCAATAAGCTGCTGTTGAAAGCAACTCCAAATACTGATCATGAGTCAAATCTTCCCAAGGAATGAAGCATGCACCTAGTGTTTCAGTGATGTTCCAAAATTTCAGTCTAGAAGAAGTGCTTCTATGTATCCCAGAACAACAAACTATGAGGGGCGTTTC
CCTAAAGAACCCATTTAACTCTACTAGTTCAGAACAGTGCCCTGGAAAATGCAACATTGGGGTATTATCTTCGGTTTCATATGCTGTCATGCACCTTCAACCTGCTATCATAATGCTACAGGACCCATTTCACTGAGATTATGCCTTCATTCATGGGCTTTGACTGGCTGGAAACTTCCCCTTTTTCCTGAGTGTTGATC
GGCATACGATTCATCCCATGGTAGATATTGATTTAGTTTTTCATCCTATAGTTCACTACAGAAGACAGAG

>TRINITY_DN48361_c1_g2_i1 from sample A10 / Example unspliced isoform:

AACTTTTCCCGCCTAGGCTAGAGATGAAGAGGGTTGCATTCACGAATTTGAAGTAGATATTAGACGAGGGCTGAGTAACTTTTCCCGCCTAGGCTAGAGATGAAGAGGGTTGCATTCACGAATTTGAAGTAGATATTAGACGAGGGCTTAGTAGCGAGGATGTCGAGAGACGGTGGAAAAAATGGGGCTGGAACAAGCTG
GAAAAGCATCCCAAGCCGTTAGTTTGGAAGCTCATCTTGGACCAATTTAGTGACACGCTGgtaagaatttttatagtgcccacttttgtttcatttgtttttgcttggattgatggcgatgaaggcggtgaaatggatatcactgctttcgtcgagcagctatttattttcttgactttgattgtcaatgctaccatcgg
tgtttagCAAGAGAGTAATGCGGAGAAAGCCTTGGAGGCTTTGAAGGAAATGCAGTCGGAACATGCCATGGTGAGAAGGGATGATAATTTCATTACGAATTTTTCTGCAGGGGGTGGCCCAAGCATGTGATCATTTTTCATCATATATGCCATCCAATATGGGAAGAGAGGGGCGCTCAAATAATCCTCCAATAAGCTGC
TGTTGAAAGCAACTCCAAATACTGATCATGAGTCAAATCTTCCCAAGGAATGAAGCATGCACCTAGTGTTTCAGTGATGTTCCAAAATTTCAGTCTAGAAGAAGTGCTTCTATGTATCCCAGAACAACAAACTATGAGGGGCGTTTCCCTAAAGAACCCATTTAACTCTACTAGTTCAGAACAGTGCCCTGGAAAATGCA
ACATTGGGGTATTATCTTCGGTTTCATATGCTGTCATGCACCTTCAACCTGCTATCATAATGCTACAGGACCCATTTCACTGAGATTATGCCTTCATTCATGGGCTTTGACTGGCTGGAAACTTCCCCTTTTTCCTGAGTGTTGATCGGCATACGATTCATCCCATGGTAGATATTGATTTAGTTTTTCATCCTATAGTT
CACTACAGAAGACAGAG