sp|Q8W4K1|TYW23_ARATH ----> tRNA wybutosine-synthesizing protein 2/3/4

Intron 1 GTGTGGTGGA ... GAGGTTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.37	65	51	66
A2	46.46	102	77	100
A3	45.04	97	66	93
A4	44.89	97	82	76
A5	43.08	37	24	32
A6	46.15	14	8	16
A7	46.28	119	84	121
A8	49.54	82	76	85
A9	44.4	72	47	68
A10	47.83	102	85	102

>TRINITY_DN45264_c0_g1_i1 from sample A10 / Example spliced isoform

CGATATTGATGTAAGGTTGTTGAGGAAAACACACCCACCCACGTAAAGAGGGCAAGGAATGAATCGGCAGGAATCTTTAATTACAACCTCCTTAGTGTTTAGGGCAGCGCAGTGTTTAGGGCAGCGCAGCTTGCGCAATCAAATTTGGAACTTTGGGGTGGGGAAACAAAGCAAGAGATTAAAAAGAGAGATCAATTTAT
GAAGTGAGGAGGTGGCAAGTGTCTCACTTCATGAGGCAGGGAGTCGGCTATGTAAAAGGAATGCTTTGCAAGATTTCCAGAGTCAATTTTCAGAGTATTTTGATTTCAGAGTCAATACATTTCCAGGGCGAGGGAGGGGGAGGATACAATGGGCAAGAAACAGAAGAAGCCCGGAACGGGAAAACAGAAAACTGAACGAA
AGACGGCCAAAGCCGAGGAGAAGCGGGGTCGCAGAGAAGAGAAGAAGATTTCGCAGGAGGACGACATTGATGCCATTCTGGCCAATATACAAAAGGAAGAGGCAAAAAGGAAAGAAGTTCATGTTGAAGAATATGTGCCACCGCCTTCACCTCGTTGCAACTGTTCAGTAACTGTGAATCCTCTAAAGGAGACCGAGTTG
ATTATATATGGGGGTGAATTTTATAATGGGGATAAGACTTTTGTATATGGAGATCTTTATAGGTACAATGCTGACAAAAGAGAATGGAAGCTGATTTCAAGTCCCAATAGTCCACCCCCTCGCAGTGCTCATCAGGCAGTTGCATGGAAAAATTCATTGTTTATTTTTGGTGGTGAATTCACTTCTCCAAATCAAGAAAG
GTTTCACCATTACAAGGATTTTTGGCGGCTTGACCTGAATACAAATCAATGGGAACAATTACAGTTAAAAGGCTCTCCCAGTGCACGATCAGGGCATCGTATGGTATTGTACAAGCACAAGATTATTATCTTTGGTGGTTTCTATGATACCTTAAGAGAAGTGAGGTATTACAATGATTTGCATGTTTTTGATCTTGATG
ATTTTAAGTGGCAAGAAATCAAACCAAAACTCGGAAGCTTATGGCCTAGTCCCAGGAGTGGTTTTCAATTTGTCGTTTACCAAGACGAGATCTTTTTATATGGTGGATATTTTAAGGACCACGTCTCAGATAAAGATGGTTCTGAGAAGGGAATTGTTCATGCAGACATGTGGGTCCTTGATCCAAAGAGTGGAGAGTGG
AATAAGGTGAAGAAGTTGGGTTTGCCTCCTGGACCACGTGCTGGATTTTCTATGTGTGTTCACAAAAAGCGGGCTCTTCTATTTGGAGGTGATGTTCTACGCAGTTTGTTCTTGAATGAGCTTTATGGCTTCCAGTTGGACAATCGGCGCTGGTATCCTTTGGAGTTAAGAAAGGGGAAAGCAGCTAAAGATAAGACACG
GGGGCAGGCAAAGACTGACCTACAATCTGGAAGACTGTCTACAAAAGGGAATGATGATGGGGATGCTCCATGTGAGTTCAAAGAAACACAGGCTTTTGGAGCTTTTGACCTTGAAGATGTGTGCCATAATAGTAGCCTTGAAATGGCAGAGGTGGATGAAGATGACTGTCAGCAATTGGTTAATAAAGTGACTGGCAAGC
TTTCTATTGGTGATCATTCTGACATCACAGAGTACAGTTCTAATGAAAAATGGGATAGCCAGAATACGAATGCTGAAGCAATGCCATTAGTTGCAACTGAGGTGGTAAAACCATGTGGACGGATCAATGCTTGCATGGTTGTTGGGAAAGATACACTCTATCTATATGGTGGCATGATGGAAATTGGTGATCAGGAAGTG
ACCTTGGATGATCTTTACTCTCTTGATCTCAACAAACTTGATGAATGGAAATGCATCATAGAGGCTTCAAAAACAGAGTGGGTAGAAGCTAGTGATGATGAAGATGATGAAAGTAATGAAGACGGAGATGTCAAAGAAAGTGATACAGAGGAGGAAGAGGAGGATGGAAGTGAGGAAGAGAAAAAAGAATCTGACAAGGG
CACTTTGGGAGTAGAAGATGCTGCTGCTATCCTAAGAGGAGAAGGTAGAAAGCTTCGCCGAAAGGAAAGACGAGCAAAAATAGACCAGATACGAGCTGAGCTGGGCCTTGCTGATTCACAGCGTACACCACTGCCGGGAGAGACACTGAAAGAGTTTTTCTCACGTACCAACTTGTACTGGCAAATGGCTGCATATGAGC
ATACTCAACACACTGGAAAGGAACTGAGAAAAGATGGCTTTGATCTTGCGGAGAGTCGCTTCAAGGAACTAAAACCTGTACTTGATGAGTTAGCTAAGCTAGAAGCAGAGCAAAAGACAGAAGAAGAAGAGGATGCAAAACACATGCTCAAGAAAAAAGGCAAAGAAAAGGCCAAGCATTCAGTTTTACATAGGTAGCTT
GGATCTGGCGTTGCCGCTTCAATTAACATTCTAATCATTTTGTTATAAGTTAAAAGCAGAACGGTCTTTGGCCTACATTCTCTGTTCCTTGGTGACTTGAATTCACAAGTCAAATGAAAGTACTTCTCTCAAGTAGTCCTCTGTCATGTAAAAAAAGAGAGGCATGCATATAGTTAAATGCTTTTTATATTCTATGTCCT
ATCTATTTTAAATGAAACGAGAGAAATAGTTCCTTGAAATGTTTACGAAAGTTCCTATAGGGATCCTGCTTTATATTTATTTCAGCTAATGGAAAAAATGTGCTGGAAGAAATATAATTAATTTTCACGAGGTTTAAGTTTGTTTGGATAGCAATATGCAGAGCAAATCCAACACTTGCATTCCAAGTCCTGAAATATGC
TCAAAAGCATCATGCAACTAGTGATGTTGAGGAACTATTTTACATATGTTTTAAGAAAGATCTCGCTAAAAGACACAATTAAAATATATTTAGAAATGCATCTTGTACACTCCAAGCATGTTGGAAGGCGGTGGC

>TRINITY_DN45264_c0_g1_i3 from sample A10 / Example unspliced isoform:

CGATATTGATGTAAGGTTGTTGAGGAAAACACACCCACCCACGTAAAGAGGGCAAGGAATGAATCGGCAGGAATCTTTAATTACAACCTCCTTAGTGTTTAGGGCAGCGCAGTGTTTAGGGCAGCGCAGCTTGCGCAATCAAATTTGGAACTTTGGGGTGGGGAAACAAAGCAAGAGATTAAAAAGAGAGATCAATTTAT
GAAGTGAGGAGGTGGCAAGTGTCTCACTTCATGAGGCAGGGAGTCGGCTATGTAAAAGGAATGCTTTGCAAGATTTCCAGAGTCAATTTTCAGAGTATTTTGATTTCAGAGTCAATACATTTCCAGGGCGAGGGAGGGGGAGGATACAATGGGCAAGAAACAGAAGAAGCCCGGAACGGGAAAACAGAAAACTGAACGAA
AGACGGCCAAAGCCGAGGAGAAGCGGGGTCGCAGAGAAGAGAAGAAGATTTCGCAGGAGGACGACATTGATGCCATTCTGGCCAATATACAAAAGGAAGAGGCAAAAAGGAAAGAAGTTCATGTTGAAGAATATGTGCCACCGCCTTCACCTCGTTGCAACTGTTCAGTAACTGTGAATCCTCTAAAGGAGACCGAGTTG
ATTATATATGGGGGTGAATTTTATAATGGGGATAAGACTTTTGTATATGGAGATCTTTATAGGTACAATGCTGACAAAAGAGAATGGAAGCTGATTTCAAGTCCCAATAGTCCACCCCCTCGCAGTGCTCATCAGGCAGTTGCATGGAAAAATTCATTGTTTATTTTTGGTGGTGAATTCACTTCTCCAAATCAAGAAAG
GTTTCACCATTACAAGGATTTTTGGCGGCTTGACCTGAATACAAATCAATGGGAACAATTACAGTTAAAAGGCTCTCCCAGTGCACGATCAGGGCATCGTATGGTATTGTACAAGCACAAGATTATTATCTTTGGTGGTTTCTATGATACCTTAAGAGAAGTGAGGTATTACAATGATTTGCATGTTTTTGATCTTGATG
ATTTTAAGTGGCAAGAAATCAAACCAAAACTCGGAAGCTTATGGCCTAGTCCCAGGAGTGGTTTTCAATTTGTCGTTTACCAAGACGAGATCTTTTTATATGGTGGATATTTTAAGGACCACGTCTCAGATAAAGATGGTTCTGAGAAGGGAATTGTTCATGCAGACATGTGGGTCCTTGATCCAAAGAGTGGAGAGTGG
AATAAGGTGAAGAAGTTGGGTTTGCCTCCTGGACCACGTGCTGGATTTTCTATGTGTGTTCACAAAAAGCGGGCTCTTCTATTTGGAGgtgtggtggatatggaggttcaagGTGATGTTCTACGCAGTTTGTTCTTGAATGAGCTTTATGGCTTCCAGTTGGACAATCGGCGCTGGTATCCTTTGGAGTTAAGAAAGGG
GAAAGCAGCTAAAGATAAGACACGGGGGCAGGCAAAGACTGACCTACAATCTGGAAGACTGTCTACAAAAGGGAATGATGATGGGGATGCTCCATGTGAGTTCAAAGAAACACAGGCTTTTGGAGCTTTTGACCTTGAAGATGTGTGCCATAATAGTAGCCTTGAAATGGCAGAGGTGGATGAAGATGACTGTCAGCAAT
TGGTTAATAAAGTGACTGGCAAGCTTTCTATTGGTGATCATTCTGACATCACAGAGTACAGTTCTAATGAAAAATGGGATAGCCAGAATACGAATGCTGAAGCAATGCCATTAGTTGCAACTGAGGTGGTAAAACCATGTGGACGGATCAATGCTTGCATGGTTGTTGGGAAAGATACACTCTATCTATATGGTGGCATG
ATGGAAATTGGTGATCAGGAAGTGACCTTGGATGATCTTTACTCTCTTGATCTCAACAAACTTGATGAATGGAAATGCATCATAGAGGCTTCAAAAACAGAGTGGGTAGAAGCTAGTGATGATGAAGATGATGAAAGTAATGAAGACGGAGATGTCAAAGAAAGTGATACAGAGGAGGAAGAGGAGGATGGAAGTGAGGA
AGAGAAAAAAGAATCTGACAAGGGCACTTTGGGAGTAGAAGATGCTGCTGCTATCCTAAGAGGAGAAGGTAGAAAGCTTCGCCGAAAGGAAAGACGAGCAAAAATAGACCAGATACGAGCTGAGCTGGGCCTTGCTGATTCACAGCGTACACCACTGCCGGGAGAGACACTGAAAGAGTTTTTCTCACGTACCAACTTGT
ACTGGCAAATGGCTGCATATGAGCATACTCAACACACTGGAAAGGAACTGAGAAAAGATGGCTTTGATCTTGCGGAGAGTCGCTTCAAGGAACTAAAACCTGTACTTGATGAGTTAGCTAAGCTAGAAGCAGAGCAAAAGACAGAAGAAGAAGAGGATGCAAAACACATGCTCAAGAAAAAAGGCAAAGAAAAGGCCAAG
CATTCAGTTTTACATAGGTAGCTTGGATCTGGCGTTGCCGCTTCAATTAACATTCTAATCATTTTGTTATAAGTTAAAAGCAGAACGGTCTTTGGCCTACATTCTCTGTTCCTTGGTGACTTGAATTCACAAGTCAAATGAAAGTACTTCTCTCAAGTAGTCCTCTGTCATGTAAAAAAAGAGAGGCATGCATATAGTTA
AATGCTTTTTATATTCTATGTCCTATCTATTTTAAATGAAACGAGAGAAATAGTTCCTTGAAATGTTTACGAAAGTTCCTATAGGGATCCTGCTTTATATTTATTTCAGCTAATGGAAAAAATGTGCTGGAAGAAATATAATTAATTTTCACGAGGTTTAAGTTTGTTTGGATAGCAATATGCAGAGCAAATCCAACACT
TGCATTCCAAGTCCTGAAATATGCTCAAAAGCATCATGCAACTAGTGATGTTGAGGAACTATTTTACATATGTTTTAAGAAAGATCTCGCTAAAAGACACAATTAAAATATATTTAGAAATGCATCTTGTACACTCCAAGCATGTTGGAAGGCGGTGGC