sp|Q6Q4D0|TONS_ARATH ----> Protein TONSOKU

Intron 1 GTATGGTTTC ... TAAGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.1	13	0	8
A2	38.1	13	1	8
A3	31.58	13	0	6
A4	0	5	0	0
A5	11.11	8	0	1
A6	0	1	0	0
A7	0	5	0	0
A8	0	3	0	0
A9	7.14	13	0	1
A10	11.11	8	0	1

>TRINITY_DN111504_c2_g1_i4 from sample A2 / Example spliced isoform

GAGCAAGTTGTGCGAGGTTGAGACTCAGTACGGCGTTTTTAGAGTCCAGAAGCTCAAATGGAATGCATGGGAACACTGCATTCATTTCGTACTAACTTCTGATTTTGCGTTTGCTAAGCAAATCCCTGAATATACACGCACCCCATGTCCTCTTGTGGGATCGACCACGAGCCTTGATCCACCCTGACTACAAAGTCTCT
CGGTCTTCAACGTGGGTGGATGATGAAACCCTATCTAACTTCTTAATATTATTTTATCTTTTAAACCCTGACGCTATTCTACTCTCTCTTCAGTTCTGTTTATTTCAATCTCACCGCGTAGTGCGTACATCCATATCTTAAAATTCGTTTCTGCAAATGCTTTGGATGGAGTTTTGGAGCCGGGCCTTTGTTGACCAAGG
AGGTGGGGTAAAGATGCTGGAAACGGGCCGTATGGAATGGGTGGAGTCGGGAAGAAGTGGGTGGAGTCGGAAGCAAATGGGATGGCTTCATATAACATTGGCAGATTGATATTTAGTTTTTTGCAAAAGATGCAATAAGACCCCCAATCCAAAGCCCTTGAGCAAATTGTACAGCTTGAAGTATTGTGAAGAAGTTGTGG
CATCTGATTGGGAGCTGGAAGACAGGTCTGCGTTGCCTCTGCTTAAGGGTCTTGTTGCAGATGGTGTATTCTCAGTCTTGGATTTGTCTTACAATTCTCTAGAGCATTATTTGATATTTATTATAGTATTTTCTCATTGCAATGGAAGAAAGTTCATCAGCTGCACAAAGCAACACACCCATTTCCAAACAGAGACCCCC
CACGGCAATGCCAAAAGACCCCCAAAACAGAGAATACAAAGAAGCAAAGAAGACTTTTGAAGCTAGGCTATGGGCAGCAGTCAAATGCACTCAATCATACAGTGGAGGAACTAGTGGGACAACTGGTTCAAAGGTTTGGTTTTGCCCTTACTATAAGGTGCAAAAGTCAGGGAGTGTGGCAAGAATTCGATGGCATTTGC
TTGGAATGGGGAAAAAAGAACAAATCAGTGTATGCATGAGTGTGCCTAATACAGAGAAAGCTTCAATACAAAAACAGTTTAATGAATTGGAAAAGTTGAAACAGTTTGCTGAAGGTTCACTTCAAACCAACAATCCAGCCACTAGAGCAATGCTAGGTGGTCCGCAAACTTCTTCTCAACTTCATGCTTCTACTTCCCAG
CTTAACAACCTATGGGATGTAAAAGGGAGAGGAAATCTTGATGCATCTGTTGGGCGTTTTTTTTTGTTGCAAATGGCATAGCTTTTAACGTCTGTAGGTCTCCTTATTGGAAGGACATGGTTAGAGATCTGGTTAACATTGCCCCGAAAGGTTACAAACCTCCTAGCTATGAAAAGATGATGACAGTAGTGTTGTCTGAA
GAGAGGACTCGGATAGAAGCTAGTCTTCATGACATTAAGGATGGTTGGAAGGACTCTGGGGTCTCCATTGTTTCAGATGGTTGGACAGATGTCAATCATCGTCCTCTTATCAATATTTTGGTTTCTAGTCCAAAAGGGGTTATGTTCTTAAAATGCCATGATACCACAGGGAAAACAAAAGATGGTCCATATTTGATGGG
TCTCTTCAAAGAAGCA

>TRINITY_DN111504_c2_g1_i7 from sample A2 / Example unspliced isoform:

GCAAATTGTACAGCTTGAAGgtatggtttcaaaagtttggtagctagagttttagctgtatagattttcctgaattgtactttcagttagtttgaaacctgtgttttacacgaatgcttgttttacagcctatttcagtgtctttgcttttgtttattaagttgcagTATTGTGAAGAAGTTGTGGCATCTGATTGGGAG
CTGGAAGACAGGTCTGCGTTGCCTCTGCTTAAGGGTCTTGTTGCAGATGGTGTATTCTCAGTCTTGGATTTGTCTTACAATTCTCTAGAGCATTATTTGATATTTATTATAGTATTTTCTCATTGCAATGGAAGAAAGTTCATCAGCTGCACAAAGCAACACACCCATTTCCAAACAGAGACCCCCCACGGCAATGCCAA
AAGACCCCCAAAACAGAGAATACAAAGAAGCAAAGAAGACTTTTGAAGCTAGGCTATGGGCAGCAGTCAAATGCACTCAATCATACAGTGGAGGAACTAGTGGGACAACTGGTTCAAAGGTTTGGTTTTGCCCTTACTATAAGGTGCAAAAGTCAGGGAGTGTGGCAAGAATTCGATGGCATTTGCTTGGAATGGGGAAA
AAAGAACAAATCAGTGTATGCATGAGTGTGCCTAATACAGAGAAAGCTTCAATACAAAAACAGTTTAATGAATTGGAAAAGTTGAAACAGTTTGCTGAAGGTTCACTTCAAACCAACAATCCAGCCACTAGAGCAATGCTAGGTGGTCCGCAAACTTCTTCTCAACTTCATGCTTCTACTTCCCAGCTTAACAACCTATG
GGATGTAAAAGGGAGAGGAAATCTTGATGCATCTGTTGGGCGTTTTTTTTTGTTGCAAATGGCATAGCTTTTAACGTCTGTAGGTCTCCTTATTGGAAGGACATGGTTAGAGATCTGGTTAACATTGCCCCGAAAGGTTACAAACCTCCTAGCTATGAAAAGATGATGACAGTAGTGTTGTCTGAAGAGAGGACTCGGAT
AGAAGCTAGTCTTCATGACATTAAGGATGGTTGGAAGGACTCTGGGGTCTCCATTGTTTCAGATGGTTGGACAGATGTCAATCATCGTCCTCTTATCAATATTTTGGTTTCTAGTCCAAAAGGGGTTATGTTCTTAAAATGCCATGATACCACAGGGAAAACAAAAGATGGTCCATATTTGATGGGTCTCTTCAAAGAAG
CA

Intron 2 GTATGGTTTC ... GTTTATTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.1	13	0	8
A2	38.1	13	1	8
A3	33.33	12	0	6
A4	0	5	0	0
A5	0	5	0	0
A6	0	1	0	0
A7	0	5	0	0
A8	0	3	0	0
A9	7.14	13	0	1
A10	11.11	8	0	1

>TRINITY_DN111504_c2_g1_i6 from sample A2 / Example spliced isoform

GAGCAAGTTGTGCGAGGTTGAGACTCAGTACGGCGTTTTTAGAGTCCAGAAGCTCAAATGGAATGCATGGGAACACTGCATTCATTTCGTACTAACTTCTGATTTTGCGTTTGCTAAGCAAATCCCTGAATATACACGCACCCCATGTCCTCTTGTGGGATCGACCACGAGCCTTGATCCACCCTGACTACAAAGTCTCT
CGGTCTTCAACTTCTGTTTATTTCAATCTCACCGCGTAGTGCGTACATCCATATCTTAAAATTCGTTTCTGCAAATGCTTTGGATGGAGTTTTGGAGCCGGGCCTTTGTTGACCAAGGAGGTGGGGTAAAGATGCTGGAAACGGGCCGTATGGAATGGGTGGAGTCGGGAAGAAGTGGGTGGAGTCGGAAGCAAATGGGA
TGGCTTCATATAACATTGGCAGATTGATATTTAGTTTTTTGCAAAAGATGCAATAAGACCCCCAATCCAAAGCCCTTGAGCAAATTGTACAGCTTGAAGTTGCAGTATTGTGAAGAAGTTGTGGCATCTGATTGGGAGCTGGAAGACAGGTCTGCGTTGCCTCTGCTTAAGGGTCTTGTTGCAGATGGTGTATTCTCAGT
CTTGGATTTGTCTTACAATTCTCTAGGTGAGGACTTGTTAAAGCTTCTCATTAATCAGGAATCTGAAATAATGTATCTGTACTTTGAAGATAAACCTACAGTAGAATATGTCTTCTTGTTTCATATTCATTTAGATAAGAACTGCATCTAAGCCTATTATCTATCTATCATATTCATAAGTTCATTCAGAGGAGACCCTA
TAACTTGAAATCAACCCTCTGTAATGCAAAATCATACATCTGCAACTCTGAATCAGACCTCTGCAGCAACTGCTAGGCAAAAGCAGTTCTTGCACATTTTATAATGGTTTTTAAACATAGTTTGCGGGTTTGCAAATCATCCTTCTGACCAAAACTGAAGACCAGACTCTATAGCTGAAAATCAACTCTCTGCAACGCAT
AATGAGACACCTACAACACATAATCAAATATTTGCAGTAACTTTTATGGAAAAATTGAAA

>TRINITY_DN111504_c2_g1_i7 from sample A2 / Example unspliced isoform:

GCAAATTGTACAGCTTGAAGgtatggtttcaaaagtttggtagctagagttttagctgtatagattttcctgaattgtactttcagttagtttgaaacctgtgttttacacgaatgcttgttttacagcctatttcagtgtctttgcttttgtttattaagTTGCAGTATTGTGAAGAAGTTGTGGCATCTGATTGGGAG
CTGGAAGACAGGTCTGCGTTGCCTCTGCTTAAGGGTCTTGTTGCAGATGGTGTATTCTCAGTCTTGGATTTGTCTTACAATTCTCTAGAGCATTATTTGATATTTATTATAGTATTTTCTCATTGCAATGGAAGAAAGTTCATCAGCTGCACAAAGCAACACACCCATTTCCAAACAGAGACCCCCCACGGCAATGCCAA
AAGACCCCCAAAACAGAGAATACAAAGAAGCAAAGAAGACTTTTGAAGCTAGGCTATGGGCAGCAGTCAAATGCACTCAATCATACAGTGGAGGAACTAGTGGGACAACTGGTTCAAAGGTTTGGTTTTGCCCTTACTATAAGGTGCAAAAGTCAGGGAGTGTGGCAAGAATTCGATGGCATTTGCTTGGAATGGGGAAA
AAAGAACAAATCAGTGTATGCATGAGTGTGCCTAATACAGAGAAAGCTTCAATACAAAAACAGTTTAATGAATTGGAAAAGTTGAAACAGTTTGCTGAAGGTTCACTTCAAACCAACAATCCAGCCACTAGAGCAATGCTAGGTGGTCCGCAAACTTCTTCTCAACTTCATGCTTCTACTTCCCAGCTTAACAACCTATG
GGATGTAAAAGGGAGAGGAAATCTTGATGCATCTGTTGGGCGTTTTTTTTTGTTGCAAATGGCATAGCTTTTAACGTCTGTAGGTCTCCTTATTGGAAGGACATGGTTAGAGATCTGGTTAACATTGCCCCGAAAGGTTACAAACCTCCTAGCTATGAAAAGATGATGACAGTAGTGTTGTCTGAAGAGAGGACTCGGAT
AGAAGCTAGTCTTCATGACATTAAGGATGGTTGGAAGGACTCTGGGGTCTCCATTGTTTCAGATGGTTGGACAGATGTCAATCATCGTCCTCTTATCAATATTTTGGTTTCTAGTCCAAAAGGGGTTATGTTCTTAAAATGCCATGATACCACAGGGAAAACAAAAGATGGTCCATATTTGATGGGTCTCTTCAAAGAAG
CA

Intron 3 GTGGGTGGAT ... CTCTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.05	3	3	5.7
A2	56.54	5.3	3	9.3
A3	40.85	6	6	2.3
A4	69.53	5.3	4	14
A5	55.11	6	7	7.3
A6	66.67	0.7	1	1
A7	-1	0	0	0
A8	16.67	2.3	0	1.3
A9	-1	0	0	0
A10	-1	0	0	0

>TRINITY_DN115441_c0_g4_i3 from sample A1 / Example spliced isoform

AGCAAGTTGTGCGAGGTTGAGACTCAGTACGGCGTTTTTAGAGTCCAGAAGCTCAAATGGAATGCATGGGAACACTGCATTCATTTCGTACTAACTTCTGATTTTGCGTTTGCTAAGCAAATCCCTGAATATACACGCACCCCATGTCCTCTTGTGGGATCGACCACGAGCCTTGATCCACCCTGACTACAAAGTCTCTC
GGTCTTCAACTTCTGTTTATTTCAATCTCACCGCGTAGTGCGTACATCCATATCTTAAAATTCGTTTCTGCAAATGCTTTGGATGGAGTTTTGGAGCCGGGCCTTTGTTGACCAAGGTTAAATTCCAAGATGTTTTCTTGTAATTCACTACCAATGTCTGTTGTGAC

>TRINITY_DN115441_c0_g4_i11 from sample A1 / Example unspliced isoform:

AGCAAGTTGTGCGAGGTTGAGACTCAGTACGGCGTTTTTAGAGTCCAGAAGCTCAAATGGAATGCATGGGAACACTGCATTCATTTCGTACTAACTTCTGATTTTGCGTTTGCTAAGCAAATCCCTGAATATACACGCACCCCATGTCCTCTTGTGGGATCGACCACGAGCCTTGATCCACCCTGACTACAAAGTCTCTC
GGTCTTCAACgtgggtggatgatgaaaccctatctaacttcttaatattattttatcttttaaaccctgacgctattctactctctcttcagTTCTGTTTATTTCAATCTCACCGCGTAGTGCGTACATCCATATCTTAAAATTCGTTTCTGCAAATGCTTTGGATGGAGTTTTGGAGCCGGGCCTTTGTTGACCAAGGT
TAAATTCCAAGATGTTTTCTTGTAATTCACTACCAATGTCTGTTGTGAC