sp|Q93Y08|AB1K8_ARATH ----> Protein ACTIVITY OF BC1 COMPLEX KINASE 8, chloroplastic

Intron 1 GTAAGTAGAC ... GTGTAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.19	12	9	10
A2	41.94	9	6	7
A3	42.86	12	10	8
A4	63.64	2	3	4
A5	33.33	3	2	1
A6	49.55	28	23	32
A7	44.24	46	35	38
A8	47.37	30	21	33
A9	51.02	41.8	49	38
A10	42.86	56	57	27

>TRINITY_DN103685_c0_g1_i3 from sample A1 / Example spliced isoform

TACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAGAATGCTTCCCAAATATTTGTTGC
AAAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTT
CTTTTTGAAGTTGGTGTTCATGGGAAGAAAATGTAGGAAAAATTAAACACAGACGATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGGCGAGTATGAAAGTTAAGTATTTTGAAATCAAGCAATTGACTGTAATGCTGTACTTGCTTTAAAATTTGAGATATGTATATGTCATTGAAACTGCGATTT
TTTTGTACTTTCTCCCTTGATCCCCATTCATGGGCACTAATATCGATATTTTGCCAGTAATATCTTTCTATTTTCTTATTGAAGAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGGGATTGGGACCTAGATTGTGTGAA
GAGAAATGCTATCAAGTTATTGATACAAGAATCGACCATCAGATTCGACCAGAGGTTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATATTCAGAAAGGTATATTAATGTTTC
AGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTAATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGATCAATTCAGTAGGCAATCATTACA
CC

>TRINITY_DN103685_c0_g1_i1 from sample A1 / Example unspliced isoform:

TACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAAATGCTTCCCAAATATTTGTTGCA
AAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAgtaagtagactctttgatagcatgttttgataagctagaaatcgttgcagcaatcccaattgaacaaagccattatgacctaatcggcttggtgaataacagttgatggttgt
cataattgaatgatgcaagagttaatagaaagcttcaagatcactgtctacaactatgaaatttttgtgattgttgacatcgttgtatggttaaaatctgttgccatcttctttctggttttcttgtttatttgatatttgtcatgtgggatcagctggcctgaccactgcccaagtaattaaagcaaaattgatatggt
aaagtgaaagaagttggaattccgtgtaagcagAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTTCTTTTTGAAGTTGGTGTTCATGGGAAGAAAATGTAGGAAAAATTAAACACAGACG
ATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGGCGAGTATGAAAGTTAAGTATTTTGAAATCAAGCAATTGACTGTAATGCTGTACTTGCTTTAAAATTTGAGATATGTATATGTCATTGAAACTGCGATTTTTTTGTACTTTCTCCCTTGATCCCCATTCATGGGCACTAATATCGATATTTTGCC
AGTAATATCTTTCTATTTTCTTATTGAAGAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGGGATTGGGACCTAGATTGTGTGAAGAGAAATGCTATCAAGTTATTGATACAAGAATCGACCATCAGATTCGACCAGAGG
TTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATATTCAGAAAGGTATATTAATGTTTCAGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTA
ATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGATCAATTCAGTAGGCAATCATTACACC

Intron 2 GCGAGTATGA ... ATTTTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.06	9	8	8
A2	44.44	5	5	3
A3	40	3	4	0
A4	100	0	2	1
A5	100	0	1	1
A6	57.01	23	38	23
A7	60.43	28.8	51.9	36
A8	63.79	21	41	33
A9	54.05	34	43	37
A10	54.84	28	52	16

>TRINITY_DN103685_c0_g1_i4 from sample A1 / Example spliced isoform

TACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAGAATGCTTCCCAAATATTTGTTGC
AAAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTT
CTTTTTGAAGTTGGTGTTCATGGGAAGAAAATGTAGGAAAAATTAAACACAGACGATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGTAATATCTTTCTATTTTCTTATTGAAGAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGAT
TGTGTAGAAGACATTGAAGGGATTGGGACCTAGATTGTGTGAAGAGAAATGCTATCAAGTTATTGATACAAGAATCGACCATCAGATTCGACCAGAGGTTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAG
GAATGAAGCCTGCACGATATTCAGAAAGGTATATTAATGTTTCAGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTAATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGC
AGATGAACGTTGTGAAGGATCAATTCAGTAGGCAATCATTACACC

>TRINITY_DN103685_c0_g1_i1 from sample A1 / Example unspliced isoform:

TACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAAATGCTTCCCAAATATTTGTTGCA
AAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAGTAAGTAGACTCTTTGATAGCATGTTTTGATAAGCTAGAAATCGTTGCAGCAATCCCAATTGAACAAAGCCATTATGACCTAATCGGCTTGGTGAATAACAGTTGATGGTTGT
CATAATTGAATGATGCAAGAGTTAATAGAAAGCTTCAAGATCACTGTCTACAACTATGAAATTTTTGTGATTGTTGACATCGTTGTATGGTTAAAATCTGTTGCCATCTTCTTTCTGGTTTTCTTGTTTATTTGATATTTGTCATGTGGGATCAGCTGGCCTGACCACTGCCCAAGTAATTAAAGCAAAATTGATATGGT
AAAGTGAAAGAAGTTGGAATTCCGTGTAAGCAGAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTTCTTTTTGAAGTTGGTGTTCATGGGAAGAAAATGTAGGAAAAATTAAACACAGACG
ATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGgcgagtatgaaagttaagtattttgaaatcaagcaattgactgtaatgctgtacttgctttaaaatttgagatatgtatatgtcattgaaactgcgatttttttgtactttctcccttgatccccattcatgggcactaatatcgatattttgcc
agTAATATCTTTCTATTTTCTTATTGAAGAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGGGATTGGGACCTAGATTGTGTGAAGAGAAATGCTATCAAGTTATTGATACAAGAATCGACCATCAGATTCGACCAGAGG
TTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATATTCAGAAAGGTATATTAATGTTTCAGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTA
ATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGATCAATTCAGTAGGCAATCATTACACC

Intron 3 GCGAGTATGA ... CTTATTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45	11	9	9
A2	44.44	5	4	4
A3	50	2	3	1
A4	0	1	0	0
A5	50	1	1	1
A6	38.46	28	19	16
A7	42.86	32	19	29
A8	41.07	33	13	33
A9	38.46	36	21	24
A10	34.35	43	26	19

>TRINITY_DN46673_c0_g3_i2 from sample A7 / Example spliced isoform

GCTGATTTGATACCGTGGGCCTGTACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAA
ATGCTTCCCAAATATTTGTTGCAAAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAGTAAGTAGACTCTTTGATAGCATGTTTTGATAAGCTAGAAATCGTTGCAGCAATCCCAATTGAACAAAGCCATTATGACCTAATCGGCTT
GGTGAATAACAGTTGATGGTTGTCATAATTGAATGATGCAAGAGTTAATAGAAAGCTTCAAGATCACTGTCTACAACTATGAAATTTTTGTGATTGTTGACATCGTTGTATGGTTAAAATCTGTTGCCATCTTCTTTCTGGTTTTCTTGTTTATTTGATATTTGTCATGTGGGATCAGCTGGCCTGACCACTGCCCAAGT
AATTAAAGCAAAATTGATATGGTAAAGTGAAAGAAGTTGGAATTCCGTGTAAGCAGAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTTCTTTTTGAAGTTGGTGTTCATGGGAAGAAAAT
GTAGGAAAAATTAAACACAGACGATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGGGATTGGGACCTAGATTGTGTGAAGAGAAATGCTATCAAG
TTATTGATACAAGAATCGACCATCAGATTCGACCAGAGGTTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATATTCAGAAAGGTATATTAATGTTTCAGCATTTGTACTTGTC
ACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTAATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGATCAATTCAGTAGGCAATCATTACACCCTTTTTTATGGGTG
CAGTTTAATTATTTTATGTTTTTATTTTTTTCG

>TRINITY_DN46673_c0_g3_i7 from sample A7 / Example unspliced isoform:

GCTGATTTGATACCGTGGGCCTGTACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAA
ATGCTTCCCAAATATTTGTTGCAAAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAGTAAGTAGACTCTTTGATAGCATGTTTTGATAAGCTAGAAATCGTTGCAGCAATCCCAATTGAACAAAGCCATTATGACCTAATCGGCTT
GGTGAATAACAGTTGATGGTTGTCATAATTGAATGATGCAAGAGTTAATAGAAAGCTTCAAGATCACTGTCTACAACTATGAAATTTTTGTGATTGTTGACATCGTTGTATGGTTAAAATCTGTTGCCATCTTCTTTCTGGTTTTCTTGTTTATTTGATATTTGTCATGTGGGATCAGCTGGCCTGACCACTGCCCAAGT
AATTAAAGCAAAATTGATATGGTAAAGTGAAAGAAGTTGGAATTCCGTGTAAGCAGAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTTCTTTTTGAAGTTGGTGTTCATGGGAAGAAAAT
GTAGGAAAAATTAAACACAGACGATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGgcgagtatgaaataatatctttctattttcttattgaagAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGG
GATTGGGACCTAGATTGTGTGAAGAGAAATGCTATCAAGTTATTGATACAAGAATCGACCATCAGATTCGACCAGAGGTTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATAT
TCAGAAAGGTATATTAATGTTTCAGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTAATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGAT
CAATTCAGTAGGCAATCATTACACCCTTTTTTATGGGTGCAGTTTAATTATTTTATGTTTTTATTTTTTTCG

Intron 4 GCGAGTATGA ... CTTATTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50.79	10.1	8	12
A2	51.96	4.5	5	4
A3	56.67	1.7	4	0
A4	65.33	0.9	2	1
A5	60	1	1	2
A6	55.54	25.9	38	25
A7	62.4	29.3	51.7	44
A8	57.31	31.8	41	44
A9	55.97	32.9	43	38
A10	50.03	39.7	52	25

>TRINITY_DN103685_c0_g1_i5 from sample A1 / Example spliced isoform

TACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAAATGCTTCCCAAATATTTGTTGCA
AAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAGTAAGTAGACTCTTTGATAGCATGTTTTGATAAGCTAGAAATCGTTGCAGCAATCCCAATTGAACAAAGCCATTATGACCTAATCGGCTTGGTGAATAACAGTTGATGGTTGT
CATAATTGAATGATGCAAGAGTTAATAGAAAGCTTCAAGATCACTGTCTACAACTATGAAATTTTTGTGATTGTTGACATCGTTGTATGGTTAAAATCTGTTGCCATCTTCTTTCTGGTTTTCTTGTTTATTTGATATTTGTCATGTGGGATCAGCTGGCCTGACCACTGCCCAAGTAATTAAAGCAAAATTGATATGGT
AAAGTGAAAGAAGTTGGAATTCCGTGTAAGCAGAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTTCTTTTTGAAGTTGGTGTTCATGGGAAGAAAATGTAGGAAAAATTAAACACAGACG
ATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGGGATTGGGACCTAGATTGTGTGAAGAGAAATGCTATCAAGTTATTGATACAAGAATCGACCAT
CAGATTCGACCAGAGGTTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATATTCAGAAAGGTATATTAATGTTTCAGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTT
CTCGTCGAAGGTTTTAATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGATCAATTCAGTAGGCAATCATTACACC

>TRINITY_DN103685_c0_g1_i3 from sample A1 / Example unspliced isoform:

TACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAGAATGCTTCCCAAATATTTGTTGC
AAAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTT
CTTTTTGAAGTTGGTGTTCATGGGAAGAAAATGTAGGAAAAATTAAACACAGACGATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGgcgagtatgaaagttaagtattttgaaatcaagcaattgactgtaatgctgtacttgctttaaaatttgagatatgtatatgtcattgaaactgcgattt
ttttgtactttctcccttgatccccattcatgggcactaatatcgatattttgccagtaatatctttctattttcttattgaagAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGGGATTGGGACCTAGATTGTGTGAA
GAGAAATGCTATCAAGTTATTGATACAAGAATCGACCATCAGATTCGACCAGAGGTTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATATTCAGAAAGGTATATTAATGTTTC
AGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTAATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGATCAATTCAGTAGGCAATCATTACA
CC

Intron 5 GTTAAGTATT ... ATTTTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.39	8	7	8
A2	50	3	3	3
A3	66.67	1	4	0
A4	100	0	2	1
A5	50	1	1	1
A6	68.18	14	37	23
A7	66.67	23	56	36
A8	68.97	18	47	33
A9	60	30	53	37
A10	69.41	13	43	16

>TRINITY_DN46673_c0_g3_i7 from sample A7 / Example spliced isoform

GCTGATTTGATACCGTGGGCCTGTACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAA
ATGCTTCCCAAATATTTGTTGCAAAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAGTAAGTAGACTCTTTGATAGCATGTTTTGATAAGCTAGAAATCGTTGCAGCAATCCCAATTGAACAAAGCCATTATGACCTAATCGGCTT
GGTGAATAACAGTTGATGGTTGTCATAATTGAATGATGCAAGAGTTAATAGAAAGCTTCAAGATCACTGTCTACAACTATGAAATTTTTGTGATTGTTGACATCGTTGTATGGTTAAAATCTGTTGCCATCTTCTTTCTGGTTTTCTTGTTTATTTGATATTTGTCATGTGGGATCAGCTGGCCTGACCACTGCCCAAGT
AATTAAAGCAAAATTGATATGGTAAAGTGAAAGAAGTTGGAATTCCGTGTAAGCAGAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAAGATGTCAAAGCTGAGGAAGCTTCTTTTTGAAGTTGGTGTTCATGGGAAGAAAAT
GTAGGAAAAATTAAACACAGACGATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGGCGAGTATGAAATAATATCTTTCTATTTTCTTATTGAAGAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGG
GATTGGGACCTAGATTGTGTGAAGAGAAATGCTATCAAGTTATTGATACAAGAATCGACCATCAGATTCGACCAGAGGTTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATAT
TCAGAAAGGTATATTAATGTTTCAGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTAATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGAT
CAATTCAGTAGGCAATCATTACACCCTTTTTTATGGGTGCAGTTTAATTATTTTATGTTTTTATTTTTTTCG

>TRINITY_DN46673_c0_g3_i3 from sample A7 / Example unspliced isoform:

GCTGATTTGATACCGTGGGCCTGTACGGGTGTAAGGTTGAAGAAAAGCGATCTGTTGTTGGGGGTTTGAATTGGAGGGTTTGAAGGTTTGTTCCATTGATCATCGGCAGCCACAGAAACCTGAAGATTTTCTCGTCCGCCCTACTTAAACCACGACTCCTCTGGCTCGAGCTCATTAACCCTGGCAGAAGCCGTATGAAA
ATGCTTCCCAAATATTTGTTGCAAAATCGCTACAAGTCATCAATGGGAAAACGTATCAGTCTACGCGCGGTTCAAAGATTTGGATAAAGGTTCCATGATTCAAGGTTTCAAAGGAAAGAGGAACGCTGCCCAAGTAAAGAAAATTGAGCATTTGCCAACGGAAGACATTTCAGAAATTGTGAAAATAATTTGATGCGGAA
GATGTCAAAGCTGAGGAAGCTTCTTTTTGAAGTTGGTGTTCATGGGAAGAAAATGTAGGAAAAATTAAACACAGACGATTTAAGCTGCAAGTCATACTTCAACGTGTTTTTCCTGGGCCCAGGCGAGTATGAAAgttaagtattttgaaatcaagcaattgactgtaatgctgtacttgctttaaaatttgagatatgta
tatgtcattgaaactgcgatttttttgtactttctcccttgatccccattcatgggcactaatatcgatattttgccagTAATATCTTTCTATTTTCTTATTGAAGAAGAAATCTCATGAAAAGTCCAACACCCTGTGAATCGTAGTCTTTGAAGGAAGCAATCGGTGCTTGTTTTGATTGTGTAGAAGACATTGAAGGG
ATTGGGACCTAGATTGTGTGAAGAGAAATGCTATCAAGTTATTGATACAAGAATCGACCATCAGATTCGACCAGAGGTTGCTTGTGTGCGTACTTCTCCTGCGGTTTGTGTTGGGTTTCGTGGTTTGTAAATGGAGAAGAAGCAGCTCGAAGTTGCGAAATGAAGTAGCTAAACACCAGGAATGAAGCCTGCACGATATT
CAGAAAGGTATATTAATGTTTCAGCATTTGTACTTGTCACAACAGATCTCAGTTTCTCTTTCTCGTCGAAGGTTTTAATAGTAATGGACTTCTGGCCTGTGAAGTGGTCTTTCTCTTCTCGGTCGATTGAAGAATATTAAGGTATCAGTCTGTCTTTGTTATAGATGCGACATTCTGGCAGATGAACGTTGTGAAGGATC
AATTCAGTAGGCAATCATTACACCCTTTTTTATGGGTGCAGTTTAATTATTTTATGTTTTTATTTTTTTCG