sp|P19242|HSP21_PEA ----> 17.1 kDa class II heat shock protein

Intron 1 GCTAGCGATT ... TTCTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	4	5
A2	13.92	34	6	5
A3	39.02	25	13	19
A4	51.22	20	23	19
A5	33.33	12	2	10
A6	23	77	20	26
A7	27.04	85	28	35
A8	33.79	48	28	21
A9	24.41	48	17	14
A10	17.81	60	15	11

>TRINITY_DN44317_c0_g1_i1 from sample A6 / Example spliced isoform

CGAATGCTCCCGAAGCGGCTCGACAGTTCTGGACGCTTCCAGAATGGATCGAAAGCCTCCATGTATTTCCTCTCCTTCTGCTTCTTCTGCATCGAGTTGTGAAGATCATTTCGCATTCTTACTCTGCAATCAATCTGCTATTTTTCAATCGTTTTTTCCAGAGCGACTGTTCGAGTTAAACTCAGAGCAAAGGATTTGAA
ATTTGGCAATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGGGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACTCGCTCTTATGTGCGTGACACCGAGGCCGTGGCTTCGACTCCCGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCCGGCCTTAAATCCAACGACATC
AAGGTTCAAGTGGAGGACGAGAACACTCTGAACATCAGTGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGTGGCGAAGTTCATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGT

>TRINITY_DN44317_c0_g1_i5 from sample A6 / Example unspliced isoform:

CGAATGCTCCCGAAGCGGCTCGACAGTTCTGGACGCTTCCAGAATGGATCGAAAGCCTCCATGTATTTCCTCTCCTTCTGCTTCTTCTGCATCGAGTTGTGAAGATCATTTCGCATTCTTACTCTGCAATCAATCTGCTATTTTTCAATCGTTTTTTCCAGAGCTACTGTTCGAGTTAAACTCAGgctagcgatttgcga
tttggttgttttcttttttatgtttttaagcaaatttcttcacaatttgaagcttgtcattgaagattatgcgggcaaataactgtattttctgtgcagAGCAAAGGATTTGAAATTTGGCAATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGGGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACT
CGCTCTTATGTGCGTGACACCGAGGCCGTGGCTTCGACTCCCGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCCGGCCTTAAATCCAACGACATCAAGGTTCAAGTGGAGGACGAGAACACTCTGAACATCAGTGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACAT
TCGCATGGAACGCAGAGTGGCGAAGTTCATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGT

Intron 2 GTATCTCATT ... TGATGCGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	8.82	62	0	6
A2	2.63	74	1	2
A3	16.67	70	3	14
A4	26.85	79	4	29
A5	10.71	25	0	3
A6	7.16	389	1	30
A7	6.35	398	0	27
A8	6.75	387	0	28
A9	6.94	362	0	27
A10	6.3	372	0	25

>TRINITY_DN44317_c0_g1_i3 from sample A6 / Example spliced isoform

CGAATGCTCCCGAAGCGGCTCGACAGTTCTGGACGCTTCCAGAATGGATCGAAAGCCTCCATGTATTTCCTCTCCTTCTGCTTCTTCTGCATCGAGTTGTGAAGAGCATTTCGCATTCTTACTCTGCAATCAGTCTGCTATTCTGCAATCGTTTTTTCCAGAGCTACTGTTCGAGTTAAACTCAGGGCAAGAGATTTGAA
ATTTGGCAATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGGGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACTCGCTCTTATGTGCGTGACACCGAGGCCGTGGCTTCGACTCCCGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCCGGCCTTAAATCCAACGACATC
AAGGTTCAAGTGGAGGACGAGAACACTCTGAACATCAGTGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGTGGCGAAGTTCATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGT

>TRINITY_DN44317_c0_g1_i2 from sample A6 / Example unspliced isoform:

CTTAAATCCAACGACATCAAGgtatctcattgcgcttgaatctccgttaagatcgctaaattttgccctattttctttcttttgttttggcagtctgctaggattttgttgaaatgtgtgttgatgcgaagGTTCAAGTGGAGGACGAGAACACTCTGAACATCAGTGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGG
GAAGTAAAATACATTCGCATGGAACGCAGAGTGGCGAAGTTCATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGT

Intron 3 GTATTTCATC ... TTAAACTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	3	0
A2	81.48	5	11	33
A3	62.26	10	6	27
A4	76.19	5	8	24
A5	57.89	4	2	9
A6	68.98	29	57	72
A7	68	28	36	83
A8	71.43	14	25	45
A9	79.44	11	37	48
A10	84.27	7	22	53

>TRINITY_DN45190_c0_g1_i8 from sample A8 / Example spliced isoform

ATTCCGAATCCGAATTCTCCCGAAGCGGCTCGACAGTACTGGACGCTTCCAGAATGGATCGAAAGCCTCCATGACAAGAGATTTGAAATTTGGCAATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGTGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACTCGCTCTTATGTGCGTGACACCGAGGCC
GTGGCTTCGACTCCCGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCCGGCCTTAAATCCAACGACATCAAGGTTCAAGTGGAGGACGAGAACACTCTGAACATCAGTGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGTGGCGAAGTT
CATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCGTCACCGTTCCCAAGCTTCCCCCGCCGGAGCCGAGGAAACCTAAGACGATTGAAGTCAAAAGTGGATAAATCCTCCGGTGTGTATGCAGAGCTCGAAATCTTTGGTGAATTTTGTGGGGTTTTG
TTGATTTTGCCGTAGCTAG

>TRINITY_DN45190_c0_g1_i2 from sample A8 / Example unspliced isoform:

ATTCCGAATCCGAATTCTCCCGAAGCGGCTCGACAGTACTGGACGCTTCCAGAATGGATCGAAAGCCTCCATgtatttcatctccttctgctgcttctgcatcgagttgtgaagagaatttcgcattcttactctgcaatcaatctgctattctgcaatcgttttttccagagctactgttcgagttaaactcagGACAA
GAGATTTGAAATTTGGCAATGGATCCTCTTTTGAGCACCGTACAGCAGCTGATCGGCGTGCCGGATGACCTGCACAGGCTCCTTAACGCCCCGACTCGCTCTTATGTGCGTGACACCGAGGCCGTGGCTTCGACTCCCGTGGATGTTAAAGAATACCCTAACTCCTACGTCTTTATAATTGACATGCCCGGCCTTAAATC
CAACGACATCAAGGTTCAAGTGGAGGACGAGAACACTCTGAACATCAGTGGTGAGCGGAAGAGGAACGAAAAGGAAGAAGGGGAAGTAAAATACATTCGCATGGAACGCAGAGTGGCGAAGTTCATGAGGAAATTCACTCTGCCTCCTGACTGCAACTTAGAGGCCATCTCTGCCGCGTGCCAGGATGGAGTGCTGACCG
TCACCGTTCCCAAGCTTCCCCCGCCGGAGCCGAGGAAACCTAAGACGATTGAAGTCAAAAGTGGATAAATCCTCCGGTGTGTATGCAGAGCTCGAAATCTTTGGTGAATTTTGTGGGGTTTTGTTGATTTTGCCGTAGCTAGATTTTGCACAATTTTGCTTTCAGGTTTGAGAAATATCCATGCAATGCAATCAATCCAT
CTAACCTGGTGGCGCACGAATAAAAAGTTTCTATATTTCTGCCATATGTTGAGTTTCCTGTTAATGGCAGAACAAAGCTCTCCATCTAAATATAATTTCCTTATTTGAAAGCTT