NA ----> NA

Intron 1 GTGAGTAATA ... TAAAACCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.53	27	26	21
A2	44.23	29	24	22
A3	45.71	38	30	34
A4	46.51	23	20	20
A5	43.86	16	14	11
A6	42.74	211	161	154
A7	45.33	243	214	189
A8	44.36	153	133	111
A9	39.38	197	153	103
A10	46.16	291	255	244

>TRINITY_DN43311_c1_g2_i2 from sample A7 / Example spliced isoform

CGGGTCAGGGCAGGATCACCGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGAC
GGCGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAATTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGA
AACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAA
ACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACC
TATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCT
GGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCATATTTCTGCTGTGATTTCTGTCATTTAATTTAATTCAGAAACTTAATTTTACTCTAT

>TRINITY_DN43311_c1_g2_i5 from sample A7 / Example unspliced isoform:

CGGGTCAGGGCAGGATCACCGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGAC
GGCGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAAgtgagtaatacggtgttttaaaacccagTTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAA
GATCGGCATGGTCTCCACCAGTCCCGGAAACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAA
AGGTGCTTTTAATATCCCGCCCAAGAAAACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGA
AAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGT
TTTCCAATTTCAAACTGATTTGGATTCTGGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCATATTTCTGCTGTGATTTCTGTCATTTAATTTAATTCAGAAACTTAATTTTACTCTAT

Intron 2 GTGAGTAATA ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.03	27	11	8
A2	26.58	29	13	8
A3	34.48	38	26	14
A4	31.34	23	12	9
A5	28.89	16	12	1
A6	32.05	211	118	81
A7	31.93	243	153	75
A8	28.67	153	89	34
A9	33.89	197	135	67
A10	28.59	291	157	76

>TRINITY_DN43311_c1_g2_i2 from sample A7 / Example spliced isoform

CGGGTCAGGGCAGGATCACCGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGAC
GGCGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAATTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGA
AACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAA
ACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACC
TATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCT
GGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCATATTTCTGCTGTGATTTCTGTCATTTAATTTAATTCAGAAACTTAATTTTACTCTAT

>TRINITY_DN43311_c1_g2_i1 from sample A7 / Example unspliced isoform:

CGGGTCAGGGCAGGATCACCGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGAC
GGCGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAAgtgagtaatacggtgttttaaaacccaggtttgcttccaaaatggcgatcttagtttcattttcatgtaaa
tagtttgaaggcaagctgctgatttacattgatggttgtttgcagTTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGAAACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAA
TGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAAACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAA
GCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCC
CGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCTGGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAAT
TTGCATATTTCTGCTGTGATTTCTGTCATTTAATTTAATTCAGAAACTTAATTTTACTCTAT

Intron 3 GTTTGCTTCC ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25	21	6	8
A2	26.67	22	8	8
A3	33.98	34	21	14
A4	27.27	20	6	9
A5	31.25	11	9	1
A6	33.91	154	77	81
A7	31.52	189	99	75
A8	30.41	111	63	34
A9	39.77	103	69	67
A10	28.13	244	115	76

>TRINITY_DN43311_c1_g2_i5 from sample A7 / Example spliced isoform

CGGGTCAGGGCAGGATCACCGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGAC
GGCGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAAGTGAGTAATACGGTGTTTTAAAACCCAGTTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAA
GATCGGCATGGTCTCCACCAGTCCCGGAAACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAATGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAA
AGGTGCTTTTAATATCCCGCCCAAGAAAACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAAGCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGA
AAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCCCGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGT
TTTCCAATTTCAAACTGATTTGGATTCTGGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAATTTGCATATTTCTGCTGTGATTTCTGTCATTTAATTTAATTCAGAAACTTAATTTTACTCTAT

>TRINITY_DN43311_c1_g2_i1 from sample A7 / Example unspliced isoform:

CGGGTCAGGGCAGGATCACCGAAAGTTTGGGGTGTGGGGTAGCGAAGGAAGAAAAATTCGACGGGATTCTTTGTATGGACGGTGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTGGATTCTTTGTATGGAC
GGCGGGTGTGGGGTAGCGAAGAAAGAAAAAAGACCGACGGGATTCTATAAATTGACGGCAGAGGAAACTGAATCGCCAATGTGGAATGAGAGATATTTTGGTTTAGTAATGCTAGATACCATTTTGGAAGTGAGTAATACGGTGTTTTAAAACCCAGgtttgcttccaaaatggcgatcttagtttcattttcatgtaaa
tagtttgaaggcaagctgctgatttacattgatggttgtttgcagTTGTGTTACTGAGAGCGGTGCCGTATAATCTGTGCCTGGAAAAGATCGGCATGGTCTCCACCAGTCCCGGAAACCCCAGGAGATTGTGCCTGACACAATCTCTTTCGTGTGTGAGAATTACGAACGATTCAACAATAACGATGGGCAAGACGAAA
TGCAGAAGAATATCCGGCTCAAGAAAGTCTCACTGGAGACTTTCCCTGAGCGATTCAACAACAGCGATGGCGAAAGATAAAAGCAGAAAGGTGCTTTTAATATCCCGCCCAAGAAAACCACTTCGGAGATTTTCGCTAAGCGATTCAACAACAGCGATGGCGAAAGGCGGATCCCTGTTTTCAACGTCGAGCACAAGAAA
GCCCCACTGGAGACCTTCCTTCAGCGATTCAAGAACAGCGGTGGCGAAGGTGAAAAGCAGAAGGATGCTTTCAATTTCAAGCCCAAGAAAGCCTCCGCTGTAGATTTTCCCTGACCTATTCAACAATATCGACGGGGAAGGTGAAATGCAAGACGATGTTTTCAATATCCCACCCAAGAAAGCTTCACCAGATATTTTCC
CGGAGCGATTCAACAACAGCGATGACGATGTTTCCAATATCCCGCCCAATAAAACCCCAAGTTTCATGTTAAGATATTTTAGAGATGTTTTCCAATTTCAAACTGATTTGGATTCTGGAACAATACAGGTTTGATTACTGTTACTTGTATCATTTTATTATCAGGATCTTTCTTTAAATATGATTTCAAGAATGTGGAAT
TTGCATATTTCTGCTGTGATTTCTGTCATTTAATTTAATTCAGAAACTTAATTTTACTCTAT