NA ----> NA

Intron 1 GTAAGGCATT ... CCCTTTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.47	29	27	88
A2	61.38	28	31	58
A3	62.91	28	32	63
A4	69.73	28	41	88
A5	54.72	24	29	29
A6	80.65	3	10	15
A7	62.32	13	9	34
A8	63.27	9	15	16
A9	48	26	27	21
A10	73.13	9	14	35

>TRINITY_DN49107_c0_g1_i6 from sample A7 / Example spliced isoform

CGCTCCTCTCTCTTCCTTATTATGTGGTTGCTCTCCTGGATAGAAGATGTCTCGGCTCCGCTCGAGGAGGACTTGCGAAGTCTTGAGAGGGATTTACTCTGCTCGAGAAGGACTTGCAGAAGTAAAGCTTCACGTAGATCTTGCAACGAAGGTCCACATGACAAGAGCTATGCTACATCGACACGACACGGCAGAAAAAC
GTACTTCTTCTGCAGATTGTACTGCCAACCGTTTTAGAAAATCAGTGTATTTCGATGCAAATCATTTGCCACTGCAAGACATTTGCAATAGTCTGTGTTCTGATGAGGCATTCAAGCTCTCACGTCTCTTCTGCATTCAACTTAATTTGAGCAAGGGTTTTCGAATCTCACATACTCTGCCGAACCCATCGGTGTATCGA
TGCAAATTATTTGCCGCTGCAAGATATTTGCAATAGGCTGTGTTCTGATGAGGCATTCAAGCTCTCACTTGTGTTCTGATGAGGCATGCGATATATAATTTATTTAGCTCTGCTGTGGCTTCGAGAAAAAAAATTCGAAAAAAGAGAGCAGGTACCGAAAATGCAGGAAAGGAAAATGAAAAATGAATGGAGAGCAGGAC
TTGCCCACTGATTTACACATTTTATTAAACCCAAGGTTCGAAGCTTGGCCTAGCACGGAATAACTGGGTTAAGGTTTGAAGTCAGAGCTTTGGTGCCATTTTAGAGGCGTTTCGGGAATTGATTAGCTTTAATGGGGCGAGAAGTGGAAGAATTGCGGATGATATTTCTTGGTAGTAAGGATTTCGGGGACCATTTTTGG
CCCAGCCGCATTGTTGCTTCTTCTTTTCAAGAGCTACTAACGAATCATCATCATCCTTGCAAGAATTACGACCGGATTTTCCGAGAAATTACAACAACGATAATCCAAGGTGGTGGTATTATGCCCCACCTTTACAGTTTACCAGGAGCTCAAAAGATACACAAGCCCCCTTAATAACTAAGCTGGGCTTAAGGACTGGT
TCTTCCCATATGCCCCCGTTCTAAAAGAGTGCGCGGGCTTGGCCGGGCATGGAGACGGCCAAGCTGCTGGATTTCGATGGCGTGAAAGTTGTGAGGAAGCGGCGGACATTTTCGTCGAGAAAGCCGAAATCGGGAGGATCACATACCTGGTTTTTCAATGAACAACATTGTTCATCGCCGTCGTCTTCCCCTTCCTCGGA
TCCTCGAAACGTTGTAAATACTGACAAGGGCTTGAACGATGCAGCCGGTAAGTCTTTAAATTCTCATTCTCACGG

>TRINITY_DN49107_c0_g1_i2 from sample A7 / Example unspliced isoform:

CGCTCCTCTCTCTTCCTTATTATGTGGTTGCTCTCCTGGATAGAAGATGTCTCGGCTCCGCTCGAGGAGGACTTGCGAAGTCTTGAGAGGGATTTACTCTGCTCGAGAAGGACTTGCAGAAGTAAAGCTTCACGTAGATCTTGCAACGAAGGTCCACATGACAAGAGCTATGCTACATCGACACGACACGGCAGAAAAAC
GTACTTCTTCTGCAGATTGTACTGCCAACCGTTTTAGAAAATCAGTGTATTTCGATGCAAATCATTTGCCACTGCAAGACATTTGCAATAGTCTGTGTTCTGATGAGGCATTCAAGCTCTCACGTCTCTTCTGCATTCAACTTAATTTGAGCAAGGGTTTTCGAATCTCACATACTCTGCCGAACCCATCGGTGTATCGA
TGCAAATTATTTGCCGCTGCAAGATATTTGCAATAGGCTGTGTTCTGATGAGGCATTCAAGCTCTCACTTGTGTTCTGATGAGGCATGCGATATATAgtaaggcattgtggattaaggtatttttctttgtggattaagttttttttaaagtttggagttaacaaaaatgataagcatctcatcttgttgaaacccttta
tagATTTATTTAGCTCTGCTGTGGCTTCGAGAAAAAAAATTCGAAAAAAGAGAGCAGGTACCGAAAATGCAGGAAAGGAAAATGAAAAATGAATGGAGAGCAGGACTTGCCCACTGATTTCCACATTCTATTAAACCCAAGGTTCGAATCCTGGCCTAGCACGGAATAACTGGGTTAAGGTTTGAAGTCAGAGCTTTGGT
GCCATTTTAGAGGCGTTTCGGGAATTGATTAGCTTTAATGGGGCGAGAAGTGGAAGAATTGCGGATGATATTTCTTGGTAGTAAGGATTTCGGGGACCATTTTTGGCCCAGCCGCATTGTTGCTTCTTCTTTTCAAGAGCTACTAACGAATCATCATCATCCTTGCAAGAATTACGACCGGATTTTCCGAGAAATTACAA
CAACGATAATCCAAGGTGGTGGTATTATGCCCCACCTTTACAGTTTACCAGGAGCTCAAAAGATACACAAGCCCCCTTAATAACTAAGCTGGGCTTAAGGACTGGTTCTTCCCATATGCCCCCGTTCTAAAAGAGTGCGCGGGCTTGGCCGGGCATGGAGACGGCCAAGCTGCTGGATTTCGATGGCGTGAAAGTTGTGA
GGAAGCGGCGGACATTTTCGTCGAGAAAGCCGAAATCGGGAGGATCACATACCTGGTTTTTCAATGAACAACATTGTTCATCGCCGTCGTCTTCCCCTTCCTCGGATCCTCGAAACGTTGTAAATACTGACAAGGGCTTGAACGATGCAGCCGGTAAGTCTTTAAATTCTCATTCTCACGG

Intron 2 GTATTTTTCT ... CCCTTTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.69	15	28	88
A2	75.22	14	27	58
A3	65.57	21	17	63
A4	82.18	14	41	88
A5	72.73	9	19	29
A6	100	0	12	15
A7	77.05	7	13	34
A8	84.92	2.5	12	16
A9	73.75	6.5	15	21
A10	78.88	6.5	13	35

>TRINITY_DN106178_c1_g1_i4 from sample A5 / Example spliced isoform

CATTTGCCGCTCCAAGATATTTGTAATACTCTGTGTTCTGATGCGGCATTCAAGCTCTCACTTGTGTTCTGATGAGGCATGCGATATATAGTAAGGCATTGTGGATTAAGATTTATTTAGCTCTGCTGTGGCTTCGAGAAAAAAAATTCGAAAAAAGAGAGCAGGTACCGAAAATGCAGGAAAGGAAAATGAAAAATGAA
TGGAGAGCAGGACTTGCCCACTGATTTCCACATTCTATTAAACCCAAGGTTCGAATCCTGGCCTAGCACGGAATAACTGGGTTAAGGTTTGAAGTCAGAGCTTTGGTGCCATTTTAGAGGCGTTTCGGGAATTGATTAGCTTTAATGGGGCGAGAAGTGGAAGAATTGCGGATGATATTTCTTGGTAGTAAGGATTTCGG
GGACCATTTTTGGCCCAGCCGCATTGTTGCTTCTTCTTTTCAAGAGCTACTAACGAATCATCATCATCCTTGCAAGAATTACGACCGGATTTTCCGAGAAATTACAACAACGATAATCCAAGGTGGTGGTATTATGCCCCACCTTTACAGTTTACCAGGAGCTCAA

>TRINITY_DN106178_c1_g1_i3 from sample A5 / Example unspliced isoform:

GGTAAGTGAATTTACATCTATGAATGCTGATCTCCTCTCACACAACGCTCCTCTCTCTTCCTTATTATGTGGTTGCTCTCCTGGATAGAAGATGTCTCGGCTCCGCTCGAGGAGGACTTGCGAAGTCTTGAGAGGGATTTACTCTGCTCGAGAAGGACTTGCAGAAGTAAAGCTTCACGTAGATCTTGCAACGAAGGTCC
ACATGACAAGAGCTATGCTACATCGACACGACACGGCAGAAAAACGTACTTCTTCTGCAGATTGTACTGCCAACCGTTTTAGAAAATCAGTGTATTTCGATGCAAATCATTTGCCACTGCAAGACATTTGCAATTGTCTGTGTTCTGATGAGGCATTCAAGCTCTCACGTCTCTTCTGCATTCAACTTAATTTGAGCAAG
GGTTTTCGAATCTCACATACTCTGCCGAACCCATCGGTGTATCGATGCAAATTATTTGCCGCTGCAAGATATTTGCAATAGGCTGTGTTCTGATGAGGCATTCAAGCTCTCACTTGTGTTCTGATGAGGCATGCGATATATAGTAAGGCATTGTGGATTAAGgtatttttctttgtggattaagttttttttaaagtttg
gagttaacaaaaatgataagcatctcatcttgttgaaaccctttatagATTTATTTAGCTCTGCTGTGGCTTCGAGAAAAAAAATTCGAAAAAAGAGAGCAGGTACCGAAAATGCAGGAAAGGAAAATGAAAAATGAATGGAGAGCAGGACTTGCCCACTGATTTCCACATTCTATTAAACCCAAGGTTCGAATCCTGGC
CTAGCACGGAATAACTGGGTTAAGGTTTGAAGTCAGAGCTTTGGTGCCATTTTAGAGGCGTTTCGGGAATTGATTAGCTTTAATGGGGCGAGAAGTGGAAGAATTGCGGATGATATTTCTTGGTAGTAAGGATTTCGGGGACCATTTTTGGCCCAGCCGCATTGTTGCTTCTTCTTTTCAAGAGCTACTAACGAATCATC
ATCATCCTTGCAAGAATTACGACCGGATTTTCCGAGAAATTACAACAACGATAATCCAAGGTGGTGGTATTATGCCCCACCTTTACAGTTTACCAGGAGCTCAA

Intron 3 GTAAGGCATT ... AAATGATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.45	32	27	75
A2	57.58	28	31	45
A3	60.61	26	31	49
A4	70.45	26	41	83
A5	65.52	15	29	28
A6	80.65	3	10	15
A7	61.76	13	9	33
A8	62.26	10	15	18
A9	60	15	27	18
A10	60.49	16	14	35

>TRINITY_DN45178_c1_g3_i1 from sample A9 / Example spliced isoform

TCTCACACAACGCTCCTCTCTCTTCCTTATTATGTGGTTGCTCTCCTGGATAGAAGATGTCTCGGCTCCGCTCGAGGAGGACTTGCGAAGTCTTGAGAGGGATTTACTCTGCTCGAGAAGGACTTGCAGAAGTAAAGCTTCACGTAGATCTTGCAACGAAGGTCCACATGACAAGAGCTATGCTACATCGACACGACACG
GCAGAAAAACGTACTTCTTCTGCAGATTGTACTGCCAACCGTTTTAGAAAATCAGTGTATTTCGATGCAAATCATTTGCCACTGCAAGACATTTGCAATTGTCTGTGTTCTGATGAGGCATTCAAGCTCTCACGTCTCTTCTGCATTCAACTTAATTTGAGCAAGGGTTTTCGAATCTCACATACTCTGCCGAACCCATC
AGTGTATCGATGCAAATTATTTGCCGCTGCAAGATATTTGCAATAGGCTGTGTTCTGATGAGGCATTCAAGCTCTCACTTGTGTTCTGATGAGGCATGCGATATATACATCTCATCTTGTTGAAACCCTTTATAGATTTATTTAGCTCTGCTGTGGCTTCGAGAAAAAAAATTCGAAAAAAGAGAGCAGGTACCGAAAAT
GCAGGAAAGGAAAATGAAAAATGAATGGAGAGCAGGACTTGCCCACTGATTT

>TRINITY_DN45178_c1_g3_i2 from sample A9 / Example unspliced isoform:

TCTCACACAACGCTCCTCTCTCTTCCTTATTATGTGGTTGCTCTCCTGGATAGAAGATGTCTCGGCTCCGCTCGAGGAGGACTTGCGAAGTCTTGAGAGGGATTTACTCTGCTCGAGAAGGACTTGCAGAAGTAAAGCTTCACGTAGATCTTGCAACGAAGGTCCACATGACAAGAGCTATGCTACATCGACACGACACG
GCAGAAAAACGTACTTCTTCTGCAGATTGTACTGCCAACCGTTTTAGAAAATCAGTGTATTTCGATGCAAATCATTTGCCACTGCAAGACATTTGCAATTGTCTGTGTTCTGATGAGGCATTCAAGCTCTCACGTCTCTTCTGCATTCAACTTAATTTGAGCAAGGGTTTTCGAATCTCACATACTCTGCCGAACCCATC
AGTGTATCGATGCAAATTATTTGCCGCTGCAAGATATTTGCAATAGGCTGTGTTCTGATGAGGCATTCAAGCTCTCACTTGTGTTCTGATGAGGCATGCGATATATAgtaaggcattgtggattaaggtatttttctttgtggattaagttttttttaaagtttggagttaacaaaaatgataagCATCTCATCTTGTTG
AAACCCTTTATAGATTTATTTAGCTCTGCTGTGGCTTCGAGAAAAAAAATTCGAAAAAAGAGAGCAGGTACCGAAAATGCAGGAAAGGAAAATGAAAAATGAATGGAGAGCAGGACTTGCCCACTGATTT